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241.
Juliana Chacón Marta Camargo de Assis Alan W. Meerow Susanne S. Renner 《Journal of Biogeography》2012,39(10):1806-1818
Aim The Alstroemeriaceae is among 28 angiosperm families shared between South America, New Zealand and/or Australia; here, we examine the biogeography of Alstroemeriaceae to better understand the climatic and geological settings for its diversification in the Neotropics. We also compare Alstroemeriaceae with the four other Southern Hemisphere families that expanded from Patagonia to the equator, to infer what factors may have permitted such expansions across biomes. Location South America, Central America, Australia and New Zealand. Methods Three chloroplast genes, one mitochondrial gene and one nuclear DNA region were sequenced for 153 accessions representing 125 of the 200 species of Alstroemeriaceae from throughout the distribution range; 25 outgroup taxa were included to securely infer evolutionary directions and be able to use both ingroup and outgroup fossil constraints. A relaxed‐clock model relied on up to three fossil calibrations, and ancestral ranges were inferred using statistical dispersal–vicariance analysis (S‐DIVA). Southern Hemisphere disjunctions in the flowering plants were reviewed for key biological traits, divergence times, migration directions and habitats occupied. Results The obtained chronogram and ancestral area reconstruction imply that the most recent common ancestor of Colchicaceae and Alstroemeriaceae lived in the Late Cretaceous in southern South America/Australasia, the ancestral region of Alstroemeriaceae may have been South America/Antarctica, and a single New Zealand species is due to recent dispersal from South America. Chilean Alstroemeria diversified with the uplift of the Patagonian Andes c. 18 Ma, and a hummingbird‐pollinated clade (Bomarea) reached the northern Andes at 11–13 Ma. The South American Arid Diagonal (SAAD), a belt of arid vegetation caused by the onset of the Andean rain shadow 14–15 Ma, isolated a Brazilian clade of Alstroemeria from a basal Chilean/Argentinean grade. Main conclusions Only Alstroemeriaceae, Calceolariaceae, Cunoniaceae, Escalloniaceae and Proteaceae have expanded and diversified from Patagonia far into tropical latitudes. All migrated northwards along the Andes, but also reached south‐eastern Brazil, in most cases after the origin of the SAAD. Our results from Alstroemeria now suggest that the SAAD may have been a major ecological barrier in southern South America. 相似文献
242.
Jotun Hein 《Journal of molecular evolution》1993,36(4):396-405
Summary Sequences subject to recombination and gene conversion defy phylogenetic analysis by traditional methods since their evolutionary history cannot be adequately summarized by a tree. This study investigates ways to describe their evolutionary history and proposes a method giving a partial reconstruction of this history. Multigene families, viruses, and alleles from within populations experience recombinations/gene conversions, so the questions studied here are relevant for a large body of data and the suggested solutions should be very practical. The method employed was implemented in a program, RecPars, written in C and was used to analyze nine retroviruses. 相似文献
243.
B. G. McCutchan J. X. Ou G. Namkoong 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1985,71(3):536-544
Summary One hundred families of average size 10 are allocated in single-tree plots to 20 blocks in several planned unbalanced designs. Based on the variance of the estimate of heritability from the Minimum Variance Quadratic Unbiased Estimates and 100% survival, the three partially balanced designs are equally efficient. A design with variable family size is more efficient for heritabilities (h2) generally less than 0.25; an equal family size design is more efficient for h2 generally greater than 0.25. A design with a large number of small families is more efficient at high h2 than that with a small number of large families; a design with fewer families of larger size is more efficient at low h2. Two-tree plot designs are never more efficient than single-tree plot designs and are also shown to be sensitive to the magnitude of the variance components that generate a given h2, whereas the single-tree plot designs are not.Paper No. 9853 of the Journal series of the North Carolina Agricultural Research Service, Raleigh, NC, USA 相似文献
244.
L. P. Lefkovitch 《Biometrical journal. Biometrische Zeitschrift》1989,31(1):75-91
If the variance, V = V(μ, ?) is some known function of the mean, μ = μ(β), where ? and β may include unknown parameters, then given empirical data, this paper describes how to estimate the unknown parameters by choosing them to satisfy the variance/mean relationship, and simultaneously to require that the sampling probability distribution has maximum entropy. Bounds for the estimated values of the unknown parameters can be obtained by a further application of the maximum entropy principle. The power variance function, V(μ)=λμ? is discussed, including some special cases of λ and ?. The procedure is briefly compared with quasi- likelihood, and illustrated by some numerical examples. 相似文献
245.
Nucleotide variation at several cold candidate genes including seven members of the dehydrin gene family was surveyed in haplotypes
of Scots pine (Pinus sylvestris) sampled in populations showing divergence for cold tolerance in Europe. Patterns of nucleotide diversity, linkage disequilibrium,
and frequency spectrum of alleles were compared between north and south populations to search for signs of directional selection
potentially underlying adaptation to cold. Significant differentiation between populations in allelic frequency or haplotype
structure was detected at dhn1, dhn3, and abaH loci. Allelic dimorphism with no evidence of haplotype clustering by geographical distribution was found at dhn9. An excess of fixed non-synonymous mutations as compared to the outgroup P. pinaster pine species was found at dhn1. Differences in nucleotide polymorphisms were found between the members of the Kn class of dehydrin upregulated during cold
acclimation (average πsil = 0.004) as compared to the SKn class (average πsil = 0.024). The multilocus nucleotide diversity at silent sites (θ
W = 0.009) was moderate compared to other conifer species, but higher than previous estimates for Scots pine. There was an
excess of rare and high frequency derived variants as revealed by significantly negative multilocus value of Tajima’s D (D = −0.72, P < 0.01) and negative mean value of Fay and Wu H statistics (H = −0.50). The level of linkage disequilibrium decayed rapidly with an average expected r
2 of 0.2 at about 200 bp. Overall, there was a positive correlation between polymorphism and divergence at ten loci when outgroup
sequence was available. The discovered polymorphism will be used for further evaluation of the adaptive role of genes through
association mapping studies.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
246.
Suites of root traits differ between annual and perennial species growing in the field 总被引:2,自引:0,他引:2
Here, we tested whether root traits associated with resource acquisition and conservation differed between life histories (annuals, perennials) and families (Fabaceae, Asteraceae and Poaceae). Root topology, morphology, chemistry and mycorrhizal colonization were measured on whole root systems of 18 field-grown herbaceous species grown and harvested in central Argentina. Annuals differed from perennials in several root traits important in resource uptake and conservation. They exhibited higher specific root length (SRL), root nitrogen concentration (RNC) and mycorrhizal colonization but had lower root tissue density (RTD) than perennials. They did not differ in topology or construction cost. These differences were consistent among families. Families differed only in a few root traits known to be strongly associated with certain lineages such as topology and nitrogen concentration. There was a strong parallel between root traits and analogous leaf traits described in the literature for annuals and perennials. Our results suggest the existence at the root level of an acquisitive vs conservative syndrome consistent among families similar to that previously reported for above-ground traits. 相似文献
247.
The contributions of specific residues to the delicate balance between function, stability and folding rates could be determined, in part by [corrected] comparing the sequences of structures having identical folds, but insignificant sequence homology. Recently, we have devised an experimental strategy to thoroughly explore residue substitutions consistent with a specific class of structure. Using this approach, the amino acids tolerated at virtually all residues of the c-Raf/Raf1 ras binding domain (Raf RBD), an exemplar of the common beta-grasp ubiquitin-like topology, were obtained and used to define the sequence determinants of this fold. Herein, we present analyses suggesting that more subtle sequence selection pressure, including propensity for secondary structure, the hydrophobic core organization and charge distribution are imposed on the Raf RBD sequence. Secondly, using the Gibbs free energies (DeltaG(F-U)) obtained for 51 mutants of Raf RBD, we demonstrate a strong correlation between amino acid conservation and the destabilization induced by truncating mutants. In addition, four mutants are shown to significantly stabilize Raf RBD native structure. Two of these mutations, including the well-studied R89L, are known to severely compromise binding affinity for ras. Another stabilized mutant consisted of a deletion of amino acid residues E104-K106. This deletion naturally occurs in the homologues a-Raf and b-Raf and could indicate functional divergence. Finally, the combination of mutations affecting five of 78 residues of Raf RBD results in stabilization of the structure by approximately 12 kJ mol(-1) (DeltaG(F-U) is -22 and -34 kJ mol(-1) for wt and mutant, respectively). The sequence perturbation approach combined with sequence/structure analysis of the ubiquitin-like fold provide a basis for the identification of sequence-specific requirements for function, stability and folding rate of the Raf RBD and structural analogues, highlighting the utility of conservation profiles as predictive tools of structural organization. 相似文献
248.
Abstract Ubiquitin ligases play an important regulatory role in the control of protein degradation processes via the ubiquitin/26S
proteasome pathway in eukaryotes. These enzymes participate in substrate specification and mediate the transfer of ubiquitin
to target proteins. A large number of ubiquitin ligases are predicted in the eukaryotes whose genomes have been sequenced;
in Arabidopsis thaliana more than 1300 genes are thought to encode ubiquitin ligases. At least three classes of ubiquitin ligases are present in
Arabidopsis, one of which comprises about 470 RING zinc-finger domain proteins. Within this class we have characterized the ATL family
that encodes a RING-H2 finger. We identified 80 members of this family in A. thaliana and 121 in Oryza sativa. About 60% of the rice ATLs are clustered with A. thaliana ATLs, and in many cases the gene products showed sequence similarities beyond the ATL’s conserved features, suggesting that
they could be orthologous genes. Ninety percent of the ATLs are intronless genes, suggesting that the structure of the basic ATL protein may have evolved as a functional module. We
carried out a survey of T-DNA insertions in 30% of the Arabidopsis ATL genes and screened for possible phenotypes. Four of these genes are likely to be essential for viability, since homozygous
plants for the T-DNA insertion were not recovered. One of them, ATL8, is mainly expressed in young siliques, suggesting a role during embryogenesis. We also recovered a line carrying a T-DNA
insertion in ATL43 that showed an ABA-insensitive phenotype, suggesting a role of this gene in the ABA response. The organization of ATLs in
Arabidopsis and rice in this study will be a valuable comprehensive guide for this multigene family.
Electronic Supplementary Material Electronic Supplementary material is available for this article at
and accessible for authorised users.
[Reviewing Editor: Dr. Martin Kreitman] 相似文献
249.
250.
There is an extensive research base on obesity treatment and on the health benefits of weight loss, but relatively little research has focused on obesity prevention. This article summarizes results of a workshop conducted by investigators funded under a National Institutes of Health initiative designed to stimulate novel research for obesity prevention. The 20 pilot studies funded under this initiative involved study populations that were diverse with respect to life stage and ethnicity, were conducted in a variety of natural and research settings, and involved a mix of interventions, including face‐to‐face group and individual counseling, as well as mail, telephone, and internet‐based approaches. The workshop, which occurred approximately halfway through the 3‐year funding period, emphasized concepts and experiences related to initiating and conducting obesity prevention studies. Investigators discussed theoretical perspectives as well as various challenges encountered, for example, in study implementation in different clinical and community settings, in working with children and families, and in studying pregnant and postpartum women. Other topics discussed included the difficulty of motivating individuals for prevention of weight gain, relevant cultural and racial/ethnic considerations, and the particular need for valid and practical measures of energy balance, body composition, and physical fitness in obesity prevention research. A key conclusion was that using obesity treatment as the primary paradigm may be a limiting perspective for considering obesity prevention issues. Further insights derived from the workshop deliberations are reflected in a detailed list of recommendations for future obesity prevention research. 相似文献