Vertical movement and time allocation of a freshwater pulmonate snail

The paper presents an analysis of locomotion and location in a vertical water column of 10Biomphalaria glabrata, under constant conditions of light, temperature, and food availability. Individual snails varied in distance traveled, and in the percentage of time spent in different areas of the water column. Distance covered ranged from 53 cm to 100 cm h^–1 . Approximately 21% of locomotion was observed to be passive (floating up or sinking down). Snails spent approximately 58% of the time in the bottom section, 35% in the top section and only 7% in the middle region. The study provides baseline data that can be used to compare further studies ofBiomphalaria glabrata as various conditions are altered.     

A developmental switch between electrical and neuropeptide communication in the ventromedial hypothalamus

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Serotonergic neurons translate taste detection into internal nutrient regulation

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Progressive bias in species status is symptomatic of fine-grained mapping units subject to repeated sampling

Atlas maps of butterflies invariably fail to distinguish the status of records, that is, whether they are observations of breeding populations or vagrant individuals. Yet, for conservation purposes, it is clearly important to know whether records relate to breeding populations in suitable habitats or not. The high mobility of butterfly adults carries two expectations. First, vagrants will frequently be recorded within grid squares, particularly when mapping is fine grained. Second, the frequency of vagrants is likely to be higher in mapping units comprising biotopes with few butterfly habitats, than in those rich in butterfly habitats, if only because there is a greater regional pool of potential vagrants for biotopes depleted in butterfly habitats. It follows that with repeated sampling of squares or biotopes differences in species breeding status will be cumulative between those rich and poor in butterfly habitats. In this paper, these predictions have been tested using data from 30, 1 ha grid squares on Alderley Edge Site of Special Scientific Interest (SSSI) (UK), half of which are in woodland (few butterfly habitats) and half with open habitats (non-woodland; rich in butterfly habitats). Butterflies have been recorded during 38 visits over 4 years. Within squares, vagrants have been distinguished from breeding species on the basis of the presence of larval hostplants and other suitable resources for seasonal maintenance. Owing to the occurrence of vagrants, number of breeding species was found to be significantly fewer than total species in mapping units. Vagrants were found to be significantly more abundant in woodland squares, which contain few species larval hostplants, than in squares dominated by open biotopes with many species larval hostplants. Repeated sampling resulted in a cumulative increase in the frequency of vagrant species. Furthermore, the relative frequency of vagrants increased at a greater rate in woodland squares compared to non-woodland squares. Suggestions are made as to how recording for atlases may be improved.     

FFAR4 regulates cardiac oxylipin balance to promote inflammation resolution in HFpEF secondary to metabolic syndrome

Heart failure with preserved ejection fraction (HFpEF) is a complex clinical syndrome, but a predominant subset of HFpEF patients has metabolic syndrome (MetS). Mechanistically, systemic, nonresolving inflammation associated with MetS might drive HFpEF remodeling. Free fatty acid receptor 4 (Ffar4) is a GPCR for long-chain fatty acids that attenuates metabolic dysfunction and resolves inflammation. Therefore, we hypothesized that Ffar4 would attenuate remodeling in HFpEF secondary to MetS (HFpEF-MetS). To test this hypothesis, mice with systemic deletion of Ffar4 (Ffar4KO) were fed a high-fat/high-sucrose diet with L-NAME in their water to induce HFpEF-MetS. In male Ffar4KO mice, this HFpEF-MetS diet induced similar metabolic deficits but worsened diastolic function and microvascular rarefaction relative to WT mice. Conversely, in female Ffar4KO mice, the diet produced greater obesity but no worsened ventricular remodeling relative to WT mice. In Ffar4KO males, MetS altered the balance of inflammatory oxylipins systemically in HDL and in the heart, decreasing the eicosapentaenoic acid-derived, proresolving oxylipin 18-hydroxyeicosapentaenoic acid (18-HEPE), while increasing the arachidonic acid-derived, proinflammatory oxylipin 12-hydroxyeicosatetraenoic acid (12-HETE). This increased 12-HETE/18-HEPE ratio reflected a more proinflammatory state both systemically and in the heart in male Ffar4KO mice and was associated with increased macrophage numbers in the heart, which in turn correlated with worsened ventricular remodeling. In summary, our data suggest that Ffar4 controls the proinflammatory/proresolving oxylipin balance systemically and in the heart to resolve inflammation and attenuate HFpEF remodeling.