全文获取类型
收费全文 | 3415篇 |
免费 | 198篇 |
国内免费 | 433篇 |
专业分类
4046篇 |
出版年
2024年 | 30篇 |
2023年 | 72篇 |
2022年 | 84篇 |
2021年 | 72篇 |
2020年 | 99篇 |
2019年 | 131篇 |
2018年 | 120篇 |
2017年 | 82篇 |
2016年 | 110篇 |
2015年 | 81篇 |
2014年 | 206篇 |
2013年 | 256篇 |
2012年 | 163篇 |
2011年 | 232篇 |
2010年 | 166篇 |
2009年 | 199篇 |
2008年 | 245篇 |
2007年 | 255篇 |
2006年 | 166篇 |
2005年 | 184篇 |
2004年 | 136篇 |
2003年 | 130篇 |
2002年 | 125篇 |
2001年 | 90篇 |
2000年 | 76篇 |
1999年 | 62篇 |
1998年 | 38篇 |
1997年 | 40篇 |
1996年 | 32篇 |
1995年 | 26篇 |
1994年 | 45篇 |
1993年 | 21篇 |
1992年 | 25篇 |
1991年 | 25篇 |
1990年 | 20篇 |
1989年 | 23篇 |
1988年 | 19篇 |
1987年 | 15篇 |
1986年 | 19篇 |
1985年 | 14篇 |
1984年 | 18篇 |
1983年 | 12篇 |
1982年 | 19篇 |
1981年 | 13篇 |
1980年 | 10篇 |
1979年 | 12篇 |
1978年 | 7篇 |
1977年 | 6篇 |
1975年 | 5篇 |
1974年 | 5篇 |
排序方式: 共有4046条查询结果,搜索用时 0 毫秒
81.
A classic question in plant ecology is “why is the world green?” That is, if plants are food for animals why do not animals eat all the available food – changing a ‘green world’ into a ‘brown world’. We first reviewed this question in 2009 and now revisit our arguments in the light of new data and new thinking. Here we argue that (1) the top–down bottom–up dichotomy is probably too simple for understanding a complex system – such as vegetation – rich in feedback processes. (2) Nevertheless it appears that bottom–up processes are generally more important for maintaining the presence of some sort of vegetation while top–down control process are generally more important in determining the type of vegetation at a site. (3) Although this review mainly takes a qualitative and experimental approach to the question, we also argue that simple well-known mathematical models from population ecology can be very informative in thinking about the types of explanations for the green world phenomenon, and demonstrating that it is rarely a simple choice between one form of control or another. 相似文献
82.
Urbanization is one of the major environmental challenges facing the world today. One of its particularly pressing effects is alterations to local and regional climate through, for example, the Urban Heat Island. Such changes in conditions are likely to have an impact on the phenology of urban vegetation, which will have knock‐on implications for the role that urban green infrastructure can play in delivering multiple ecosystem services. Here, in a human‐dominated region, we undertake an explicit comparison of vegetation phenology between urban and rural zones. Using satellite‐derived MODIS‐EVI data from the first decade of the 20th century, we extract metrics of vegetation phenology (date of start of growing season, date of end of growing season, and length of season) for Britain's 15 largest cities and their rural surrounds. On average, urban areas experienced a growing season 8.8 days longer than surrounding rural zones. As would be expected, there was a significant decline in growing season length with latitude (by 3.4 and 2.4 days/degree latitude in rural and urban areas respectively). Although there is considerable variability in how phenology in urban and rural areas differs across our study cities, we found no evidence that built urban form influences the start, end, or length of the growing season. However, the difference in the length of the growing season between rural and urban areas was significantly negatively associated with the mean disposable household income for a city. Vegetation in urban areas deliver many ecosystem services such as temperature mitigation, pollution removal, carbon uptake and storage, the provision of amenity value for humans and habitat for biodiversity. Given the rapid pace of urbanization and ongoing climate change, understanding how vegetation phenology will alter in the future is important if we wish to be able to manage urban greenspaces effectively. 相似文献
83.
84.
Yasunori Sugiyama Sho Yamashita Yuuki Uezato Yukako Senga Syouichi Katayama Naoki Goshima Yasushi Shigeri Noriyuki Sueyoshi Isamu Kameshita 《Analytical biochemistry》2016
To analyze a variety of protein phosphatases, we developed phosphorylated TandeMBP (P-TandeMBP), in which two different mouse myelin basic protein isoforms were fused in tandem, as a protein phosphatase substrate. P-TandeMBP was prepared efficiently in four steps: (1) phosphorylation of TandeMBP by a protein kinase mixture (Ca2+/calmodulin-dependent protein kinase Iδ, casein kinase 1δ, and extracellular signal-regulated kinase 2); (2) precipitation of both P-TandeMBP and protein kinases to remove ATP, Pi, and ADP; (3) acid extraction of P-TandeMBP with HCl to remove protein kinases; and (4) neutralization of the solution that contains P-TandeMBP with Tris. In combination with the malachite green assay, P-TandeMBP can be used to detect protein phosphatase activity without using radioactive materials. Moreover, P-TandeMBP served as an efficient substrate for PPM family phosphatases (PPM1A, PPM1B, PPM1D, PPM1F, PPM1G, PPM1H, PPM1K, and PPM1M) and PPP family phosphatase PP5. Various phosphatase activities were also detected with high sensitivity in gel filtration fractions from mouse brain using P-TandeMBP. These results indicate that P-TandeMBP might be a powerful tool for the detection of protein phosphatase activities. 相似文献
85.
Effects of heat and drought stress on post‐illumination bursts of volatile organic compounds in isoprene‐emitting and non‐emitting poplar 下载免费PDF全文
Werner Jud Elisa Vanzo Ziru Li Andrea Ghirardo Ina Zimmer Thomas D. Sharkey Armin Hansel Jörg‐Peter Schnitzler 《Plant, cell & environment》2016,39(6):1204-1215
Over the last decades, post‐illumination bursts (PIBs) of isoprene, acetaldehyde and green leaf volatiles (GLVs) following rapid light‐to‐dark transitions have been reported for a variety of different plant species. However, the mechanisms triggering their release still remain unclear. Here we measured PIBs of isoprene‐emitting (IE) and isoprene non‐emitting (NE) grey poplar plants grown under different climate scenarios (ambient control and three scenarios with elevated CO2 concentrations: elevated control, periodic heat and temperature stress, chronic heat and temperature stress, followed by recovery periods). PIBs of isoprene were unaffected by elevated CO2 and heat and drought stress in IE, while they were absent in NE plants. On the other hand, PIBs of acetaldehyde and also GLVs were strongly reduced in stress‐affected plants of all genotypes. After recovery from stress, distinct differences in PIB emissions in both genotypes confirmed different precursor pools for acetaldehyde and GLV emissions. Changes in PIBs of GLVs, almost absent in stressed plants and enhanced after recovery, could be mainly attributed to changes in lipoxygenase activity. Our results indicate that acetaldehyde PIBs, which recovered only partly, derive from a new mechanism in which acetaldehyde is produced from methylerythritol phosphate pathway intermediates, driven by deoxyxylulose phosphate synthase activity. 相似文献
86.
GENE SILENCING BY PARENTAL RNA INTERFERENCE IN THE GREEN RICE LEAFHOPPER,Nephotettix cincticeps (HEMIPTERA: CICADELLIDAE) 下载免费PDF全文
RNA interference (RNAi) has been widely used for investigating gene function in many nonmodel insect species. Parental RNAi causes gene knockdown in the next generation through the administration of double‐strand RNA (dsRNA) to the mother generation. In this study, we demonstrate that parental RNAi mediated gene silencing is effective in determining the gene function of the cuticle and the salivary glands in green rice leafhopper (GRH), Nephotettix cincticeps (Uhler). Injection of dsRNA of NcLac2 (9 ng/female) to female parents caused a strong knockdown of laccase‐2 gene of first instar nymphs, which eventually led to high mortality rates and depigmentation of side lines on the body. The effects of parental RNAi on the mortality of the nymphs were maintained through 12–14 days after the injections. We also confirmed the effectiveness of parental RNAi induced silencing on the gene expressed in the salivary gland, the gene product of which is passed from instar to instar. The parental RNAi method can be used to examine gene function by phenotyping many offspring nymphs with injection of dsRNA into a small number of parent females, and may be applicable to high‐efficiency determination of gene functions in this species. 相似文献
87.
88.
城市热岛(UHI)不仅直接关系到城市人居环境质量和居民健康状况,同时还对城市能源消耗、生态系统过程演变、生物物候以及城市经济可持续发展有着深远的影响。以北京市朝阳区2002—2017年夏季4期Landsat系列遥感影像为数据,采用大气反演法,对15年间朝阳区城市地表温度(LST)时空变化进行分析,发现15年间朝阳区城市热岛比例指数上升迅速,热岛效应逐年加剧。进一步研究表明,城市地表温度与归一化植被指数(NDVI)及归一化建筑指数(NDBI)密切相关:地表温度与NDBI正相关,NDBI指数每升高0.1,地表升温0.79~2.37°C;与NDVI指数负相关,NDVI指数每提高0.1,地表降温0.4~0.77°C。本研究可为城市规划建设与城市绿地营建提供科学具体的参考指导依据,并促进生态可持续发展与人居环境改善。 相似文献
89.
植物作为自然界生态系统的重要组成部分,其多样性的保护与发展是人类可持续发展的物质基础之一,对维护生态环境的平衡与稳定具有重要意义,也是当今中国生态园林城市建设的重要内容。以上海滨江森林公园一期工程为例,通过总结建设过程中自然生境的恢复与重建、现状植物保护和开发的经验,探索郊野公园设计建造中植物多样性保护的有效措施,为今后城市郊野公园的建设和原有绿地的绿化改造等提供参考借鉴。 相似文献
90.