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61.
62.
Flotation of lipid-protein particles containing triacylglycerol and phospholipid from the cytosol of carnation petals 总被引:2,自引:0,他引:2
Lipid-protein particles ranging from 20 to 250 nm in diameter have been isolated from the cytosol of carnation petals by flotation centrifugation and also by ultrafiltration. The cytosolic lipid-protein particles resemble oil bodies, lipid-protein particles found in oil-bearing seeds, in that they contain triacylglycerol, are circumscribed by phospholipid that is not organized in a bilayer, appear to be derived from membranes and can be isolated by flotation. However, the cytosolic particles are distinguishable from oil bodies in that triacylglycerol is not the dominant lipid. Indeed, they contain a spectrum of lipids in addition to phospholipids and triacylglycerol including free fatty acids, sterol and wax esters, phosphatidic acid and diacylglycerol. These same lipids are present in corresponding microsomal membranes as well, but in much smaller proportions relative to phospholipid. The lipid-protein particles from carnation petals contain a 17-kDa protein that is of similar size to oil body oleosin, but does not cross-react with anti-oleosin antibodies. The data indicate that these cytosolic particles are structurally and chemically similar to oil bodies and are consistent with the notion that their genesis may be a means of removing destabilizing lipids from membrane bilayers. 相似文献
63.
Torné J. M. Rodriguez P. Manich A. Claparols I. Santos M. A. 《Plant Cell, Tissue and Organ Culture》1997,51(2):95-102
The embryogenic capacity of Araujia sericifera petals and some of the factors involved in the induction of embryos was investigated.
The influence of 6-benzyladenine and α-naphthalene acetic acid, light intensity (90 or 5 μmol m-2 s-1) and silver thiosulphate (inhibitor of ethylene action) were studied. It was found that petals are an easy system in which
to induce somatic embryogenesis. Plants were recovered from somatic embryos. Although 6-benzyladenine is essential for inducing
an efficient response, a high dosage increased callogenesis and reduced embryogenesis. The highest rate of embryogenesis is
induced with high light intensity (90–100 μmol m-2 s-1), even though the presence of silver thiosulphate in the medium markedly reduced embryo induction.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
64.
65.
In the presence of ethylene, which enhances carnation flower senescence, carbohydrates contribute to ovary growth not only from the stem and calyx but also from the petals. With silver thiosulphate and ethanol treatments which delay flower senescence, the petals remain the active sink and ovary development is suppressed. Ethylene stimulated chloroplast development in the ovary wall. However, the calyx plus stem of all treatments showed the greater photosynthetic ability and transported a major portion of the synthesised products to the ovary. 相似文献