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71.
Carroll D. Arnett Joanna S. Fowler Robert R. MacGregor David J. Schlyer Alfred P. Wolf Bengt Långström Christer Halldin 《Journal of neurochemistry》1987,49(2):522-527
The distribution of carbon-11-labeled L-deprenyl, an irreversible inhibitor of monoamine oxidase type B (MAO-B), was determined in the baboon brain by positron emission tomography. The irreversible blood-to-brain transfer constant (influx constant, Ki) was measured using a complete metabolite-corrected arterial plasma concentration curve. This influx constant was used as a measure of functional enzyme activity for sequential determinations of MAO-B recovery following a single high dose of unlabeled l -deprenyl. The half-life for turnover of MAO-B was thus determined to be 30 days. Using appropriate irreversible inhibitors, this procedure should be generally useful for determining enzyme turnover rates in any organ in vivo and can be applied to some human studies as well. 相似文献
72.
The fate of15N labeled nitrogen applied to mature citrus trees 总被引:1,自引:0,他引:1
Summary The efficiency and balance of nitrogen from one year's application was studied in a long-term fertigation experiment. Enriched
nitrogen fertilizer, K15NO3, was applied to a 22-year-old Shamouti orange tree with a history of high N applications (N3) and to an N-starved tree (N1). The distribution of N in the different parts of the trees and in the soil was determined after the experimental trees were
excavated.
Similar total recovery of the labeled fertilizer N was found in the trees and soil in both treatments (N1−61.7% N3−56%). However, the distribution between tree and soil was different. The amount of recovered residual fertilizer in the soil
was much larger in the N3 treatment than in N1. The highest percentage of fertilizer N was found in the new organs,i.e. fruits, twigs and leaves. The roots and branches took up only 6–14% from the labeled fertilizer.
Only 20.9% of the leaf N and 23.4% of the fruit N in the N3 tree originated in the labeled fertilizer, indicating translocation of N from older parts of the tree to new growth.
Evidence was found of storage of N in the wooded branches, while the roots contained a surprisingly small part of labeled
fertilizer.
Contribution 1599E. 相似文献
73.
Five plant species were cultivated on a soil from the Neckar alluvial fan near Heidelberg (FRG) polluted by the emissions
of a cement plant. Thallium, cadmium and lead concentrations in seedlings and mature plants were determined by atomic absorption
analysis. AdditionallyBrassica napus L.napus was grown on soils containing 5 different concentrations of heavy metals, achieved by mixing two similar soils, from the
same area but with different metal concentrations.
Thallium and cadmium were shown to be taken up by roots whilst lead which was also absorbed, was deposited mainly on the plant
surface. However during cultivation in the winter months, a remarkable deposit of lead via the roots was found. Thallium in
the soil from a anthrorogen source was more available to plants than thallium of geological origin.
During the lifetime of a plant concentrations of thallium and cadmium were always highest in the seedling. The decrease in
metal concentration with maturity depended on the plant species and the element, but was not a function of the metal concentration
in the soil. 相似文献
74.
Initial (Fo), maximum (Fm) and steady-state (Fs) levels of modulated chlorophyll fluorescence were measured in intact avocado leaves (Persea americana Mill.) during state 1-state 2 transitions using a combination of modulated and non-modulated lights with synchronized detection. Under normal temperature conditions (20°C), transition from state 2 to state 1 was associated with a substantial increase (about 20%) in Fm and Fo whereas the Fm/Fo ratio remained constant, reflecting increased absorption cross-section of PS II. On the contrary, at moderately elevated temperature (35°C), these fluorescence changes were very limited, indicating marked inhibition of the state regulation. The fraction of light distributed to PS II () was calculated from the Fo, Fm and Fs levels for both types of leaves. In control leaves, varied from 48% (in state 2) to values as high as 58% (in state 1). In contrast, mild heat treatment resulted in values close to 50% in both states, indicating the inability of heated leaves to reach extreme state 1. The results suggested that avocado leaves under moderately elevated temperature conditions are blocked in a state close to state 2. This effect was shown to occur in a non-injurious temperature range (as shown by the preservation of the (photoacoustically monitored) oxygen evolution activity) and to be rapidly reversed upon lowering of the temperature. Thermally induced development of state 2 (independent on the light spectral quality) could possibly be a protective mechanism to avoid photodamage of the heat-labile PS II by high light intensities which usually accompany heat stress in the field. 相似文献
75.
S. K. Podder A. Chakraborti K. Vijayalakshmi P. Lalit Kumar Singh 《Journal of biosciences》1987,11(1-4):495-502
To examine how surface Potential controls the reactivity of glycoconjugates at cell surface, the interaction of galactose-sPecific
lectinse.g. peanut agglutinin,Ricinus cummunis agglutinin with liPosomes bearing asialo GM1 were studied in the Presence of varying amount of ganglioside mixture, GMn. The Presence of 5% GMn causes comPlete slowing down of PreciPitin reaction and thereby make carbohydrate moiety of asialo GM1 comPletely inaccessiblei.e. ‘cryPtic’. In contrast the Presence of 1–2% GMn enhances the aPParent rate and amPlitude of the PreciPitin reaction as surface Potential becomes more negative. The relevance
of the findings has been discussed in relation to the exPression and involvement of the cell-surface sialic acid residues
during develoPment and differentiation. 相似文献
76.
Ten species of Lepadella Bory de St. Vincent, 1826, including one new species and one new form, are documented from North-Eastern India. Two of these species are new records from this country and six are new reports from N.E. region. Comments are also made on the status and distribution of various Indian taxa. 相似文献
77.
Michael F. Hammer James W. Schilling Ellen M. Prager Allan C. Wilson 《Journal of molecular evolution》1987,24(3):272-279
Summary Two major types of lysozymec (M and P) occur in the mouse genus,Mus, and have been purified from an inbred laboratory strain (C58/J) ofM. domesticus. They differ in physical, catalytic, and antigenic properties as well as by amino acid replacements at 6 of 49 positions in the amino-terminal sequence. Comparisons with four other mammalian lysozymesc of known sequence suggest that M and P are related by a gene duplication that took place before the divergence of the rat and mouse lineages. M lysozyme is present in most tissues; achieves its highest concentration in the kidney, lung, and spleen; and corresponds to the lysozyme partially sequenced before from another strain ofM. domesticus. InM. domesticus and several related species, P lysozyme was detected chiefly in the small intestine, where it is probably produced mainly by Paneth cells. A survey of M and P levels in 22 species of muroid rodents (fromMus and six other genera) of known phylogenetic relationships suggests that a mutation that derepressed the P enzyme arose about 4 million years ago in the ancestor of the housemouse group of species. Additional regulatory shifts affecting M and P levels have taken place along lineages leading to other muroid species. Our survey of 187 individuals of wild house mice and their closest allies reveals a correlation between latitude of origin and level of intestinal lysozyme. 相似文献
78.
黑龙江省三江平原丹顶鹤的数量分布 总被引:2,自引:0,他引:2
在地面调查的基础上,我们使用Y-11轻型飞机对黑龙江省三江平原地区的丹顶鹤的数量分布近行了调查,调查时飞行高度80米,航速140公里/小时,续航里程共3748公里。调查结果表明,丹顶鹤在三江平原主要分布在8个地区,其中嘟噜河下游、洪河自然保护区、七星河流域和兴凯湖低地是主要繁殖地,总数量共309只。 相似文献
79.
The phytogeographical territories of Israel and Sinai are revised using a large mass of species distribution data. With the help of chorotype frequencies for 25 km2 individual squares in Israel, territories were defined on the basis of the first two most frequent chorotypes. The similarity of boundaries in the resulting phytogeographical map with a physiognomic map suggests that the latter may be used in preliminary stages of the preparation of plant geographical maps. 相似文献
80.
Types I and IV collagenolytic and plasminogen activator activities in preovulatory ovarian follicles 总被引:1,自引:0,他引:1
A Palotie T Salo K K Vihko L Peltonen H Rajaniemi 《Journal of cellular biochemistry》1987,34(2):101-112
During ovulation, enzymatic degradation of the extracellular matrix occurs within and around the graafian follicles. In this study, the activities of several different proteolytic enzymes were measured in the culture media of follicles taken from pregnant mare serum gonadotropin (PMSG)-primed immature rats. At 52 h after PMSG, the follicles were cultured for 2 to 15 h in media with or without human chorionic gonadotropin (hCG). Type I collagenase activity in hCG-stimulated follicles gradually increased within 6 h to 3.3-fold above that of the controls. Relatively pure populations of granulosa cells produced type I collagenase to a similar extent. Likewise, type IV collagenase increased 3.8-fold by 6 h after exposure of the follicles to hCG. In contrast, plasminogen activator activity increased by 3.9-fold at 2 h after hCG, but was negligible at 4, 6, and 15 h after incubation. These results suggest that plasminogen activator may activate both type I and type IV collagenase in hCG-stimulated ovulatory follicles. 相似文献