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21.
Muscle compression commonly occurs in daily life (for instance wearing backpacks or compression garments, and during sitting). However, the effects of the compression on contraction dynamics in humans are not well examined. The aim of the study was to quantify the alterations of contraction dynamics and muscle architecture in human muscle with external transverse loads.The posterior tibialis nerve of 29 subjects was stimulated to obtain the maximal double-twitch force of the gastrocnemius muscle with and without transverse compression that was generated using an indentor. The muscle architecture was determined by a sonographic probe that was embedded within the indentor. Five stimulations each were conducted at 5 conditions: (1) pretest (unloaded), (2) indentor loading with 2 kg, (3) 4.5 kg, (4) 10 kg, and (5) posttest (unloaded).Compared to the pretest maximal force decreased by 9%, 13% and 16% for 2 kg, 4.5 kg and 10 kg, respectively. The half-relaxation time increased with increased transverse load whereas the rate of force development decreased from pretest to 2 kg and from 4.5 kg to 10 kg. The lifting height of the indentor increased with transverse load from 2 kg to 4.5 kg but decreased from 4.5 kg to 10 kg. Increases in pennation during the twitches were reduced at the highest transverse load.The results demonstrate changes of the contraction dynamics due to transversal muscle loading. Those alterations are associated with the applied pressure, changes in muscle architecture and partitioning of muscle force in transversal and longitudinal direction. 相似文献
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23.
Philip D. Chilibeck Cheryl R. McCreary Greg D. Marsh Donald H. Paterson Earl G. Noble Albert W. Taylor R. Terry Thompson 《European journal of applied physiology and occupational physiology》1998,78(5):460-465
The purpose of this study was to compare muscle oxidative capacity between moderately active young and old humans by measuring
intracellular threshold (IT) during exercise with 31P-magnetic resonance spectroscopy (31P-MRS). Changes in phosphocreatine, inorganic phosphate, and intracellular pH were measured by 31P-MRS during a progressive unilateral ankle plantar flexion exercise protocol in groups of moderately active old (n=12, mean age 66.7 years) and young (n=13, mean age 26.2 years) individuals. From muscle biopsy samples of the lateral gastrocnemius, citrate synthase (CS) activity
was determined in six subjects from each group, and fibre type composition was determined in nine old and ten young subjects.
The old group had a lower IT for pH, as a percentage of peak work rate (P<0.05), despite a similar CS activity compared to the young. IT was significantly correlated with CS activity (R=0.59; P<0.05), but not with fibre type composition. It was concluded that metabolic responses to exercise are affected by ageing,
as indicated by a lower IT in old compared to young individuals.
Accepted: 7 May 1998 相似文献
24.
Renato Chaves Souto Branco Rafael Ludemann Camargo Thiago Martins Batista Jean Franciesco Vettorazzi Camila Lubaczeuski Lucas Henrique Montes Bomfim Leonardo Reis Silveira Antônio Carlos Boschero Cláudio Cesar Zoppi Everardo Magalhães Carneiro 《Journal of cellular physiology》2019,234(5):6313-6323
Nutrient malnutrition, during the early stages of development, may facilitate the onset of metabolic diseases later in life. However, the consequences of nutritional insults, such as a high-fat diet (HFD) after protein restriction, are still controversial. We assessed overall glucose homeostasis and molecular markers of mitochondrial function in the gastrocnemius muscle of protein-restricted mice fed an HFD until early adulthood. Male C57BL/6 mice were fed a control (14% protein-control diet) or a protein-restricted (6% protein-restricted diet) diet for 6 weeks. Afterward, mice received an HFD or not for 8 weeks (mice fed a control diet and HFD [CH] and mice fed a protein-restricted diet and HFD [RH]). RH mice showed lower weight gain and fat accumulation and did not show an increase in fasting plasma glucose and insulin levels compared with CH mice. RH mice showed higher energy expenditure, increased citrate synthase, peroxisome-proliferator-activated receptor gamma coactivator 1-alpha protein content, and higher levels of malate and α-ketoglutarate compared with CH mice. Moreover, RH mice showed increased AMPc-dependent kinase and acetyl coenzyme-A (CoA) carboxylase phosphorylation, lower intramuscular triacylglycerol content, and similar malonyl-CoA levels. In conclusion, protein undernourishment after weaning does not potentiate fat accumulation and insulin resistance in adult young mice fed an HFD. This outcome seems to be associated with increased skeletal muscle mitochondrial oxidative capacity and reduced lipids accumulation. 相似文献
25.
Induction of microRNA-138 by pro-inflammatory cytokines causes endothelial cell dysfunction 总被引:1,自引:0,他引:1
Exposure to pro-inflammatory cytokines, such as Angiotensin II, endothelin-1 or TNF leads to endothelial dysfunction, characterized by the reduced production of nitric oxide via endothelial nitric oxide synthase (eNOS). We recently identified the Ca2+ binding protein S100A1 as an essential factor required for eNOS activity. Here we report that pro-inflammatory cytokines down-regulate expression of S100A1 in primary human microvascular endothelial cells (HMVECs) via induction of microRNA-138 (miR-138), in a manner that depends on the stabilization of HIF1-α. We show that loss of S100A1 in ECs reduces stimulus-induced NO production, which can be prevented by inhibition of miR-138. Our study suggests that targeting miR-138 might be beneficial for the treatment of cardiovascular disease. 相似文献
26.
Leterme D Casasnovas B 《European journal of applied physiology and occupational physiology》1999,79(4):312-317
Contractile and fatigue-resistance properties of 71 lateral gastrocnemius muscle (LG) motor units (MU) following 14 days of hindlimb unloading (HU) were compared to those of 60 LG MU from control rats. The MU properties were assessed from isolated and stimulated individual motor axons. The MU were classified using standard criteria (shape of unfused tetani and fatigue resistance). The HU did not affect LG MU composition, but diminished the maximal tetanic tension (Po) of all MU types: P0 was significantly reduced by about 40% for the slow and fast-resistant MU, and by 18% for the fast-fatigable ones. The speed-related properties of fast-resistant MU became more similar to those of slower MU. The fatigue properties of MU were evaluated during a 5-min exercise test, using two fatigue indexes, FI2 and FI5, which expressed the relative capacity of MU to generate tension after 2 and 5 min, respectively. Results showed that 14 days of HU did not change the fatigue sensitivity of the LG MU. However, when F15 was compared to FI2, a greater decrease was observed after HU than in control conditions for the fast-resistant and fast-intermediate MU. It was concluded that a prolonged fatigue test may show changes in metabolic properties of muscle fibres during 14 days of HU. Specific adaptations of LG MU as well as comparisons with those of the soleus muscle under the same conditions are discussed. 相似文献
27.
S. Hejazi J. Rasmussen 《Computer methods in biomechanics and biomedical engineering》2017,20(2):130-141
This paper presents a finite element model of the ankle, taking into account the effects of muscle forces, determined by a musculoskeletal analysis, to investigate the contact stress distribution in the tibio-talar joint in patients with triple arthrodesis and in normal subjects. Forces of major ankle muscles were simulated and corresponded well with the trend of their EMG signals. These forces were applied to the finite element model to obtain stress distributions for patients with triple arthrodesis and normal subjects in three stages of the gait cycle, i.e. heel strike, midstance, and heel rise. The results demonstrated that the stress distribution patterns of the tibio-talar joint in patients with triple arthrodesis differ from those of normal subjects in investigated gait cycle stages. The mean and standard deviations for maximum stresses in the tibo-talar joint in the stance phase for patients and normal subjects were 9.398e7 ± 1.75e7 and 7.372e7 ± 4.43e6 Pa, respectively. The maximum von Mises stresses of the tibio-talar joint for all subjects in the stance phase found to be on the lateral side of the inferior surface of the joint. The results also indicate that, in patients with triple arthrodesis, increasing gastrocnemius–soleus muscle force reduces the stress on the medial malleolus compared with normal subjects. Most of stresses in this area are between 45 and 109 kPa, and will decrease to almost 32 kPa in patients after increasing of 40% in gastrocnemius–soleus muscle force. 相似文献
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Determinants of diaphragmatic injury 总被引:1,自引:0,他引:1
Severe muscle wasting is a characteristic feature of sepsis. We have previously established that the rate of protein synthesis in muscles composed of fast-twitch fibers is severely diminished in response to sepsis. The present studies investigate the biochemical reactions responsible for the decreased rate of protein synthesis using gastrocnemius from control and septic rats perfused in situ. Analysis of free ribosomal subunits indicated peptide-chain initiation was impaired by infection. To characterize biochemical reactions in the pathway of peptide-chain initiation affected, the effect of sepsis on the incorporation of initiator [35S]methionyl-tRNA (met-tRNAi
mec) into the 40S initiation complex was examined. Sepsis caused a 65% decrease in the binding of radiolabelled met-tRNAi
mec to the 40S initiation complex compared with controls. The binding of met-tRNAmec to the 40S ribosome is regulated by eukaryotic initiation factor eIF-2B, whose activity can be modulated in part by the redox state of pyridine dinucleotides. The mean cytoplasmic NADH/NAD+ ratio was increased 2 fold in sepsis, while the NADPH/NADP+ ratio was unchanged. These findings identify the formation of the 40S initiation complex as a defect in the protein synthesis machinery during sepsis. The decreased formation of the 40S initiation complex in muscle could not be explained by changes in the cytoplasmic redox state. 相似文献
30.
O. S. Tarasova V. U. Kalenchuk A. A. Borzykh A. A. Andreev-Andrievsky S. V. Buravkov A. P. Sharova O. L. Vinogradova 《Biophysics》2008,53(6):621-625
Characteristics of the small arteries (with a diameter of 200-250 μm) feeding the medial gastrocnemius muscle and diaphragm were studied. Recording of the mechanical activity of ring segments under isometric conditions demonstrated that, similar to other arteries feeding the muscles with a high content of slow fibers, the diaphragm arteries are highly sensitive to adrenoceptor agonists and acetylcholine. The differences in the endothelium-dependent relaxation in response to acetylcholine were retained in the presence of L-NAME and diclofenac. The diaphragm and gastrocnemius arteries similarly responded to serotonin. On the other hand, a high innervation density was characteristic of the diaphragm arteries unlike the arteries of other slow muscles. The density of adrenergic nerve plexus in the diaphragm arteries was considerably higher than in the gastrocnemius arteries. The results suggest that the characteristics of small diaphragm arteries are determined not only by the oxidative capacity of diaphragm muscle fibers, but also by the fact that this is a respiratory muscle. 相似文献