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41.
Hu L Wu X Han J Chen L Vass SO Browne P Hall BS Bot C Gobalakrishnapillai V Searle PF Knox RJ Wilkinson SR 《Bioorganic & medicinal chemistry letters》2011,21(13):3986-3991
A series of nitrobenzyl phosphoramide mustards and their analogs was designed and synthesized to explore their structure-activity relationships as substrates of nitroreductases from Escherichia coli and trypanosomes and as potential antiproliferative and antiparasitic agents. The position of the nitro group on the phenyl ring was important with the 4-nitrobenzyl phosphoramide mustard (1) offering the best combination of enzyme activity and antiproliferative effect against both mammalian and trypanosomatid cells. A preference was observed for halogen substitutions ortho to benzyl phosphoramide mustard but distinct differences were found in their SAR of substituted 4-nitrobenzyl phosphoramide mustards in E. coli nitroreductase-expressing cells and in trypanosomatids expressing endogenous nitroreductases. 相似文献
42.
大蒜根系分泌物的化感作用 总被引:6,自引:0,他引:6
以苍山白蒜和蔡家坡紫蒜为材料,采用水培方法收集根系分泌物,研究了2个大蒜品种的根系分泌物对莴苣种子发芽和幼苗生长及对黄瓜枯萎病菌、西瓜枯萎病菌的化感效应.结果表明:2个大蒜品种的根系分泌物对莴苣种子发芽和幼苗生长均表现为低浓度(0.1、0.2 g·mL-1)促进、高浓度(0.4、0.6 g·mL-1)抑制,高浓度时蔡家坡紫蒜的抑制作用大于苍山白蒜;对黄瓜枯萎病菌和西瓜枯萎病菌的菌丝生长及孢子萌发均表现为抑制作用,随着根系分泌物浓度的提高,抑制作用增强,其中黄瓜枯萎病菌较敏感,且蔡家坡紫蒜的抑制作用大于苍山白蒜. 相似文献
43.
芥子气暴露的确证:家兔皮肤染毒后血红蛋白N端缬氨酸加合物的同位素稀释-NCI-GC/MS溯源性检测 总被引:1,自引:0,他引:1
素有"毒剂之王"之称的芥子气(HD)是目前危害最大的化学战剂之一,染毒后在体内可产生不同类型的特征性生物标志物,其对中毒诊断、溯源性分析以及毒理机制等研究有着重要的意义.本文首先采用同位素稀释-NCI-GC/MS分析方法监测和鉴定了HD体外全血染毒后产生的血红蛋白N端缬氨酸加合物(HETE-Val);其次,研究了不同剂量HD(0.02~0.15LD50)经皮染毒家兔体内HETE-Val的时效、量效关系.结果表明,HETE-Val与家兔中毒剂量间有良好的量效关系,而时效关系表明染毒后15min内即可产生并检测到HETE-Val,一周内达到最大,随后逐渐下降,但至染毒后第103天仍可监测到该加合物.与血红蛋白N端缬氨酸加合反应的HD仅占HD染毒总量的~0.15‰,与家兔血液反应的HD约占HD染毒总量的~1%.表明HETE-Val是一种重要的生物标志物,并可应用于HD暴露的确证和化学武器核查的溯源性检测. 相似文献
44.
Sun H Li N Wang X Chen T Shi L Zhang L Wang J Wan T Cao X 《Molecular and cellular biochemistry》2005,275(1-2):85-94
Oxygen-free radicals and other oxygen/nitrogen species are constantly generated in the human body. Most are intercepted by antioxidant defences and perform useful metabolic roles, whereas others escape to damage biomolecules like DNA, lipids and proteins. Garlic has been shown to contain antioxidant phytochemicals that prevent oxidative damage. These include unique water-soluble organosulphur compounds, lipid-soluble organosulphur compounds and flavonoids. Therefore, in the present study, we have tried to explore the antioxidant effect of garlic supplementation on oxidative stress-induced DNA damage, nitric oxide (NO) and superoxide generation and on the total antioxidant status (TAS) in patients of essential hypertension (EH). Twenty patients of EH as diagnosed by JNC VI criteria (Group I) and 20 age and sex-matched normotensive controls (Group II) were enrolled in the study. Both groups were given garlic pearls (GP) in a dose of 250 mg per day for 2 months. Baseline samples were taken at the start of the study, i.e. 0 day, and thereafter 2 months follow-up. 8-Hydroxy-2′-deoxyguanosine (8-OHdG), lipids, lipid peroxidation (MDA), NO and antioxidant vitamins A, E and C were determined. A moderate decline in blood pressure (BP) and a significant reduction in 8-OHdG, NO levels and lipid peroxidation were observed in Group I subjects with GP supplementation. Further, a significant increase in vitamin levels and TAS was also observed in this group as compared to the control subjects. These findings point out the beneficial effects of garlic supplementation in reducing blood pressure and counteracting oxidative stress, and thereby, offering cardioprotection in essential hypertensives. 相似文献
45.
Nidhi?P.?ChananaEmail author Vibha?Dhawan Sant?S.?Bhojwani 《Plant Cell, Tissue and Organ Culture》2005,83(2):169-177
Androgenesis is a phenomenon in which microspores are made to bypass the sexual pathway and follow the sporophytic mode of development to generate new plants without the intervention of fertilization under specialized in vitro conditions. Microspore culture provides an ideal system, with a large, relatively uniform population of haploid cells, for use in mutant selection, genetic transformation and in studies on the molecular mechanism of induction of androgenesis and embryogenesis. This paper involves a study on establishing a reproducible and efficient protocol for microspore embryogenesis in various varieties of Brassica juncea. The genotype had a pronounced effect on androgenic response in microspore cultures. The cultivar Rajat exhibited the most response, producing around 3500 embryos/100 buds. The microspores of B. juncea cv. PR-45 from ed plants maintained at a day/night temperature of 10 °C/5 °C form embryos with suspensors with varied morphology. The microspore embryos germinated to produce plants with frequencies. These plants exhibited 52% survival and 74% fertility. 相似文献
46.
47.
The isolation and structure determination of phomapyrones D-G, three 2-pyrones and a coumarin, from a group of isolates of the fungal pathogen Leptosphaeria maculans (Desm.) Ces. et de Not., asexual stage Phoma lingam (Tode ex Fr.) Desm, is reported. As well, phomenin B, infectopyrone, and polanrazines B and C were also obtained for the first time from these isolates. In addition, based on results of incorporations of 13C-labeled acetate and malonate, and deuterated methionine, a polyketide pathway is proposed for the biosyntheses of phomapyrones. 相似文献
48.
Qabar A Nelson M Guzman J Corun C Hwang BJ Steinberg M 《Journal of biochemical and molecular toxicology》2005,19(4):213-225
We compared the effects of overexpressing a tightly regulated anti-inflammatory cytokine, interleukin 10 (IL-10), and the pro-inflammatory cytokine tumor necrosis factor-alpha (TNF-alpha) on sulfur mustard induced cytotoxicity in human epidermal keratinocytes. Both cytokines were overexpressed when compared with the cells transfected with the empty vector as determined by quantitative ELISA. Cells overexpressing interleukin 10 suppressed the pro-inflammatory cytokines interleukin 8 and interleukin 6 following exposure to 50-300 microM sulfur mustard. These cells exhibited delayed onset of sulfur mustard induced cell death. On the other hand, cells overexpressing tumor necrosis factor alpha induced a sustained elevation in both interleukin 6 and 8 expression following exposure to 50-300 microM sulfur mustard. These cells were sensitized to the effects of sulfur mustard that resulted in an increased sulfur mustard induced cell death. Normal human epidermal keratinocytes treated with sulfur mustard exhibited elevated levels of tumor necrosis factor alpha expression and increased activity of nuclear factor kappa B. Gene array data indicated that cells overexpressing interleukin 10 induced several genes that are involved in growth promotion and cell-fate determination. We, therefore, identify IL-10 and TNF-alpha signal transduction pathways and their components as possible candidates for early therapeutic intervention against sulfur mustard induced cell injury. 相似文献
49.
Sourdeval M Lemaire C Deniaud A Taysse L Daulon S Breton P Brenner C Boisvieux-Ulrich E Marano F 《Apoptosis : an international journal on programmed cell death》2006,11(9):1545-1559
In the present study, the toxicity of yperite, SM, and its structural analogue mechlorethamine, HN2, was investigated in a
human bronchial epithelial cell line 16HBE. Cell detachment was initiated by caspase-2 activation, down-regulation of Bcl-2
and loss of mitochondrial membrane potential. Only in detached cells, mustards induced apoptosis associated with increase
in p53 expression, Bax activation, decrease in Bcl-2 expression, opening of the mitochondrial permeability transition pore,
release of cytochrome c, caspase-2, -3, -8, -9 and -13 activation and DNA fragmentation. Apoptosis, occurring only in detached cells, could be recognized
as anoikis and the mitochondrion, involved both in cell detachment and subsequent cell death, appears to be a crucial checkpoint.
Based on our understanding of the apoptotic pathway triggered by mustards, we demonstrated that inhibition of the mitochondrial
pathway by ebselen, melatonin and cyclosporine A markedly prevented mustard-induced anoikis, pointing to these drugs as interesting
candidates for the treatment of mustard-induced airway epithelial lesions.
This work was support by the Délégation Générale pour l’Armement (D.G.A./D.S.P. No. 95-151). A. Deniaud received a fellowship
from Ligue contre le Cancer. C. Brenner is supported by the Association pour la Recherche sur le Cancer (ARC). The authors
are grateful to D.C. Gruenert for providing us with the human bronchial epithelial cell line. 相似文献
50.
四川冬菜中细菌群落组成及多样性 总被引:1,自引:0,他引:1
【目的】了解腌制4年的四川南充冬菜中细菌群落组成及多样性。【方法】通过16S rDNA多样性分析样品细菌落组成;采用16S rDNA-RFLP方法分析从样品中分离出的纯培养细菌。【结果】16S rDNA多样性分析结果表明,样品中细菌主要属于变形杆菌门(Proteobacteria)和厚壁菌门(Firmicutes),分别占克隆文库的87.9%、7.1%,其中包括Virgibacillus kekensis,Marinococcus albus,Salinicoccus sp.,Lactobacillus halophilus和Halomonas等中度嗜盐菌,仅有5%属于放线菌门(Actinobacteria)。通过纯培养方法从冬菜中分离到35株菌,16S rDNA-RFLP分析结果表明,34株属于厚壁菌门(Firmicutes),包括Virgibacillus,Bacillus megaterium和Gracilibacillus saliphilus等中度嗜盐菌,1株属于放线菌门(Actinobacteria)。【结论】冬菜中细菌群落多样性较低,以中度嗜盐菌为主。 相似文献