全文获取类型
收费全文 | 3048篇 |
免费 | 224篇 |
国内免费 | 321篇 |
专业分类
3593篇 |
出版年
2023年 | 55篇 |
2022年 | 56篇 |
2021年 | 103篇 |
2020年 | 115篇 |
2019年 | 132篇 |
2018年 | 107篇 |
2017年 | 104篇 |
2016年 | 104篇 |
2015年 | 113篇 |
2014年 | 154篇 |
2013年 | 188篇 |
2012年 | 133篇 |
2011年 | 135篇 |
2010年 | 102篇 |
2009年 | 130篇 |
2008年 | 145篇 |
2007年 | 146篇 |
2006年 | 132篇 |
2005年 | 91篇 |
2004年 | 114篇 |
2003年 | 118篇 |
2002年 | 98篇 |
2001年 | 99篇 |
2000年 | 79篇 |
1999年 | 57篇 |
1998年 | 73篇 |
1997年 | 72篇 |
1996年 | 62篇 |
1995年 | 56篇 |
1994年 | 54篇 |
1993年 | 56篇 |
1992年 | 33篇 |
1991年 | 37篇 |
1990年 | 33篇 |
1989年 | 37篇 |
1988年 | 23篇 |
1987年 | 30篇 |
1986年 | 22篇 |
1985年 | 27篇 |
1984年 | 37篇 |
1983年 | 16篇 |
1982年 | 13篇 |
1981年 | 20篇 |
1980年 | 11篇 |
1979年 | 16篇 |
1978年 | 12篇 |
1977年 | 9篇 |
1976年 | 10篇 |
1974年 | 9篇 |
1973年 | 5篇 |
排序方式: 共有3593条查询结果,搜索用时 0 毫秒
91.
92.
Many symbioses have costs and benefits to their hosts that vary with the environmental context, which itself may vary in space. The same symbiont may be a mutualist in one location and a parasite in another. Such spatially conditional mutualisms pose a dilemma for hosts, who might evolve (higher or lower) horizontal or vertical transmission to increase their chances of being infected only where the symbiont is beneficial. To determine how transmission in hosts might evolve, we modeled transmission evolution where the symbiont had a spatially conditional effect on either host lifespan or fecundity. We found that over ecological time, symbionts that affected lifespan but not fecundity led to high frequencies of infected hosts in areas where the symbiont was beneficial and low frequencies elsewhere. In response, hosts evolved increased horizontal transmission only when the symbiont affected lifespan. We also modeled transmission evolution in symbionts, which evolved high horizontal and vertical transmission, indicating a possible host–symbiont conflict over transmission mode. Our results suggest an eco‐evolutionary feedback where the component of host fitness affected by a conditionally mutualistic symbiont in turn determines its distribution in the population, and, through this, the transmission mode that evolves. 相似文献
93.
Lithocarols A-F (1–6) possessing novel highly-oxygenated isobenzofuran core, together with a related known compound isoprenylisobenzofuran A (7) were isolated from the marine-derived fungus Phomopsis lithocarpus FS508. Among them, lithocarols A-E (1–5) represent the first examples of poly-ketal derivatives in tenellone family. The structures for all these compounds were fully elucidated by spectroscopic analysis, X-ray diffraction, and electronic circular dichroism calculations. Their cytotoxic assay disclosed that compounds 1–4 displayed moderate growth inhibitory effect against four human tumor cell lines with the IC50 values ranging from 10.5 to 38.7 μM. 相似文献
94.
95.
Bazyli Czeczuga Krystyna Brzozowska Lucyna Woronowicz 《International Review of Hydrobiology》1990,75(2):245-255
The authors investigated the mycoflora and the environmental factors in the River Czarna Hańcza (10 stations) and its tributary River Marycha (1 station) as well as Lakes Hańcza (2 stations) and Wigry (2 stations) on the occurrence of various aquatic fungi. At the stations investigated the presence of 45 aquatic fungi species was noted. The following fungi, up to now unknown in Poland, were found: Monoblepharis hypogyna, Rozellopsis inflata, Cladolegnia eccentrica, Apostemidium guernisaci, Anguillospora gigantea, Geniculospora grandis, Clavariopsis aquatica, Lemonniera aquatica and Lemonniera terrestris. 相似文献
96.
Purification and characteristics of cytosolic chitinase from Piromyces communis OTS1 总被引:2,自引:0,他引:2
Masaru Sakurada Diego P. Morgavi Kenji Komatani Yoshifumi Tomita ryoji Onodera 《FEMS microbiology letters》1996,137(1):75-78
Abstract A chitinase was purified from the cytosolic fraction of the anaerobic rumen fungus Piromyces communis OTS1 by affinity chromatography using regenerated chitin, gel filtration and chromatofocusing. The chitinase was most active at pH 6.2 and at 60 °C in a 20-min assay. The molecular mass of the purified protein was estimated by SDS-PAGE to be 42 kDa and its pI was 4.9. The enzyme activity, which was of the 'endo' type, was inhibited by A+ , Hg2+ and allosamidin. N -Acetyl- β -glucosaminidase and 'exo' type chitinase activity were absent from the purified preparation. 相似文献
97.
William S. Clayton 《International Review of Hydrobiology》1984,69(5):633-642
The effects of light regime, feeding regime and tentacle number on the zooplankton feeding capability of Hydra viridis were tested in the laboratory. Feeding was measured by exposing Hydra to a known volume of Artemia salina nauplii and recording the number captured and ingested. In all cases there was a correlation between the number of Artemia captured and the number ingested. H. viridis with 7 tentacles captured and ingested more Artemia than Hydra with 6 tentacles. However, changes in light and/or feeding regimes did not alter the number of tentacles/Hydra. Varying light and feeding regimes altered the number of Zoochlorellae/cell and Hydra growth rate. There was no effect on the number of Artemia captured or ingested and no effect on the percent ingestion of captured Artemia. These data suggest that, under these conditions, zooplankton feeding by H. viridis is independent of nutritional history. 相似文献
98.
Competition strategies for the decolorization of a textile-reactive dye with the white-rot fungi Trametes versicolor under non-sterile conditions 总被引:11,自引:0,他引:11
A variety of white-rot fungi can oxidize textile dyes under sterile conditions; however, an important consideration for their use in treating wastewater containing textile dyes is whether similar degrees of treatment can be achieved under non-sterile conditions. Four strategies were investigated for their potential in optimizing the use of the fungus Trametes versicolor in non-sterile culture for treating wastewater containing the diazo textile dye C.I. Reactive Black 5 (RB5). Three strategies with suspended culture were designed to increase the decolorization activity in suspended culture from a given amount of T. versicolor inoculum based on its tolerance of low pH (pH reduction in medium), production of extracellular enzymes (use of suspended enzymes alone), and its ability to produce enzymes independent of growth (nitrogen limitation in medium). The results showed that reduction of the medium pH to 3 did not suppress bacterial growth, while enzyme production by T. versicolor ceased. The use of the extracellular enzymes alone would allow the decoupling of the process of fungal growth from wastewater treatment; however, the enzyme activity of an enzyme suspension decreased rapidly under non-sterile conditions. The strategy of limiting nitrogen in the medium to suppress bacterial growth has potential together with the fourth strategy, the cultivation of fungi on organic solids to produce inocula for a decolorization process under non-sterile conditions. A high degree of decolorization of RB5 under non-sterile conditions was achieved with T. versicolor grown on grains as sole substrate. The rate of decolorization was dependent on the amount of fungal inoculum used. 相似文献
99.
100.
Extracellular proteases produced by Scytalidium thermophilum, grown on microcrystalline cellulose, were most active at pH 6.5–8 and 37–45 °C when incubated for 60 min. Highest protease activity was at day 3 where endoglucanase activity was low. Protease activity measurements with and without the protease inhibitors, p-chloromercuribenzoate, PMSF, antipain, E-64, EDTA and pepstatin A, suggest production of thiol-containing serine protease and serine proteases. Endoglucanase and Avicel-adsorbable endoglucanase activity in culture medium was not significantly affected by protease inhibitors. 相似文献