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71.
Abram F Enright AM O'Reilly J Botting CH Collins G O'Flaherty V 《Journal of applied microbiology》2011,110(6):1550-1560
Aims: The objective of this work was to provide functional evidence of key metabolic pathways important for anaerobic digestion processes through the identification of highly expressed proteins in a mixed anaerobic microbial consortium. Methods and Results: The microbial communities from an anaerobic industrial‐like wastewater treatment bioreactor were characterized using phylogenetic analyses and metaproteomics. Clone libraries indicated that the bacterial community in the bioreactor was diverse while the archaeal population was mainly composed of Methanocorpusculum‐like (76%) micro‐organisms. Three hundred and eighty‐eight reproducible protein spots were obtained on 2‐D gels, of which 70 were excised and 33 were identified. The putative functions of the proteins detected in the anaerobic bioreactor were related to cellular processes, including methanogenesis from CO2 and acetate, glycolysis and the pentose phosphate pathway. Metaproteomics also indicated, by protein assignment, the presence of specific micro‐organisms in the bioreactor. However, only a limited overlap was observed between the phylogenetic and metaproteomic analyses. Conclusions: This study provides some direct evidence of the microbial activities taking place during anaerobic digestion. Significance and Impact of Study: This study demonstrates metaproteomics as a useful tool to uncover key biochemical pathways underpinning specific anaerobic bioprocesses. 相似文献
72.
Nuptial gift chemistry reveals convergent evolution correlated with antagonism in mating systems of harvestmen (Arachnida,Opiliones)
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Penelope C. Kahn Dennis D. Cao Mercedes Burns Sarah L. Boyer 《Ecology and evolution》2018,8(14):7103-7110
Nuptial gifts are material donations given from male to female before or during copulation and are subject to sexual selection in a wide variety of taxa. The harvestman genus Leiobunum has emerged as a model system for understanding the evolution of reproductive morphology and behavior, as transitions between solicitous and antagonistic modes of courtship have occurred multiple times within the lineage and are correlated with convergence in genital morphology. We analyzed the free amino acid content of nuptial gift secretions from five species of Leiobunum using gas chromatography–mass spectrometry. Multivariate analysis of the free amino acid profiles revealed that, rather than clustering based on phylogenetic relationships, nuptial gift chemical composition was better predicted by genital morphology and behavior, suggesting that convergent evolution has acted on the chemical composition of the nuptial gift. In addition, we found that, species with solicitous courtship produce gifts consisting of a 19% larger proportion of essential amino acids as compared to those with more antagonistic courtship interactions. This work represents the first comparative study of nuptial gift chemistry within a phylogenetic framework in any animal group and as such contributes to our understanding of the evolution of reproductive diversity and the participant role of nuptial gift chemistry in mating system transitions. 相似文献
73.
自噬在病原真菌生殖中的作用 总被引:1,自引:0,他引:1
自噬是真核生物中重要且高度保守的蛋白降解过程。在此过程中,细胞中的细胞器、长寿蛋白及其他大分子物质被双层膜的自噬体包裹并运送至降解细胞器中进行降解并重新利用。自噬在病原真菌诸如细胞分化、营养动态平衡以及致病性等各种细胞过程中起重要作用。在本综述中,我们简要介绍了自噬过程,并以人体病原真菌新生隐球菌为例介绍了病原真菌的有性生殖过程;同时我们也总结了目前模式病原真菌中自噬相关基因的研究情况以及自噬调控病原真菌无性和有性生殖的可能机理;最后我们总结全文并讨论了未来自噬调控真菌有性生殖机理研究的工作方向。 相似文献
74.
75.
Mukhopadhyay K Garrison NK Hinton DM Bacon CW Khush GS Peck HD Datta N 《Mycopathologia》1996,134(3):151-159
We isolated seven different bacteria from rice seedlings grown from surface sterilized seeds. Three were associated with the rice seed husk and the other four were growing endophytically within the seed. Microscopic studies revealed that the endophytes were concentrated in the root stele region. Some of the bacteria exhibited strong anti-fungal activity against Rhizoctonia solani, Pythium myriotylum, Guamannomyces graminis and Heterobasidium annosum. 相似文献
76.
Phylogenetic convergence and multiple shell shape optima for gliding scallops (Bivalvia: Pectinidae)
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J. M. Serb E. Sherratt A. Alejandrino D. C. Adams 《Journal of evolutionary biology》2017,30(9):1736-1747
An important question in evolutionary biology is how often, and to what extent, do similar ecologies elicit distantly related taxa to evolve towards the same phenotype? In some scenarios, the repeated evolution of particular phenotypes may be expected, for instance when species are exposed to common selective forces that result from strong functional demands. In bivalved scallops (Pectinidae), some species exhibit a distinct swimming behaviour (gliding), which requires specific biomechanical attributes to generate lift and reduce drag during locomotive events. Further, a phylogenetic analysis revealed that gliding behaviour has independently evolved at least four times, which raises the question as to whether these independent lineages have also converged on a similar phenotype. Here, we test the hypothesis that gliding scallops display shell shape convergence using a combination of geometric morphometrics and phylogenetic comparative methods that evaluate patterns of multivariate trait evolution. Our findings reveal that the gliding species display less morphological disparity and significant evolutionary convergence in morphospace, relative to expectations under a neutral model of Brownian motion for evolutionary phenotypic change. Intriguingly, the phylomorphospace patterns indicate that gliding lineages follow similar evolutionary trajectories to not one, but two regions of morphological space, and subsequent analyses identified significant differences in their biomechanical parameters, suggesting that these two groups of scallops accomplish gliding in different ways. Thus, whereas there is a clear gliding morphotype that has evolved convergently across the phylogeny, functionally distinct morphological subforms are apparent, suggesting that there may be two optima for the gliding phenotype in the Pectinidae. 相似文献
77.
报道1例由棕黑腐质霉属(Humicola fuscoatra)导致的真菌性腹膜炎。此菌分离自1名长期腹膜透析患者的腹水。腐质霉属在自然界广泛存在,棕黑腐质霉导致的人类感染罕见。现对棕黑腐质霉的真菌学特点进行研究,并进行分子测序。体外药物敏感性试验结果对伊曲康唑的MIC为0.008μg/mL,伏立康唑的MIC为0.016μg/mL,两性霉素B的MIC为1.5μg/mL。患者拔除腹透管,改行血液透析。口服伊曲康唑0.1 g/12 h,28 d后病情明显改善,出院。 相似文献
78.
松树蜂与其共生真菌的互利共生关系 总被引:3,自引:0,他引:3
松树蜂Sirex noctilio Fabricius是一种重要的国际林业检疫性害虫,主要危害针叶树,原产欧亚大陆和北非。近100多年来,先后入侵大洋洲(新西兰和澳大利亚)、南美洲(乌拉圭、阿根廷、巴西和智利)、北美洲(加拿大和美国),以及南非。2013年8月,在中国黑龙江省内首次发现松树蜂,目前发现其主要危害樟子松。松树蜂能与一种淀粉韧革菌属Amylostereum的真菌Amylostereum areolatum(Fr.)Boidin形成严格的互利共生关系,该虫除直接钻蛀树木外,还能通过产卵行为将自身毒素腺体分泌的毒素和体内共生真菌随同虫卵一起注入寄主树木体内,形成"虫-毒-菌"3个致害因子相互协作的特殊危害方式,加速树势的衰弱并造成寄主树木死亡。本文就国内外松树蜂与其共生菌互利共生关系的研究进行了综述,分别从结构与功能的层次上对其互利共生关系进行了梳理和总结,重点阐释了松树蜂与共生菌的营养共生关系,松树蜂携带传播共生菌的机制,共生菌的种群遗传学以及松树蜂毒素和共生菌在危害寄主树木时的协同关系等。以期为开展关于松树蜂的专项研究提供一些合理的建议,同时为积极有效地防控该害虫提供科学依据。 相似文献
79.
Taxonomy is a traditional subject, but it still receives attention and has become a topic of much discussion in recent years. Many of these discussions have raised concerns about the future of taxonomy, especially with regard to the workforce responsible for the discovery of new species in the context of declining biodiversity. Previous discussions were based on the taxonomic data of plants and animals, but the status of fungal taxonomy has not been mentioned. Fungi have one of the highest levels of biodiversity among all living organisms, second only to insects. The discussion of the future of taxonomy without the inclusion of fungal data is incomplete. Here, we present the results of analyses based on all new fungal taxa published since 1753. Fungal taxonomy is an ever‐growing area of study with increasing numbers of new taxa being described and growing numbers of fungal taxonomists. Compared with plants and most animal groups, there has been a much sharper increase in the rate at which new fungal taxa are being described. Furthermore, the number of taxonomists studying fungi has increased at a faster speed than those studying plants or animals. This indicates that fungal taxonomy is a prosperous subject and a dynamic area for scientific studies, and that it deserves much more attention and support. The study of fungal taxonomy will deepen our understanding of the biodiversity of our planet. 相似文献
80.
在黄花蒿(Artemisia annua L.)发根液体培养中,黄花蒿内生炭疽菌(Colletotrichum sp. B501)细胞壁寡糖提取物可促进发根青蒿素的合成.经寡糖诱导子(20 mg/L)处理4 d后,发根青蒿素含量达1.15 mg/g, 比对照高出64.29%.诱导作用与诱导子浓度、作用时间相关.诱导处理1 d后,X射线能谱分析表明黄花蒿发根细胞中Ca2+积累量显著增高,电镜观察发现液泡内出现高电子致密物,具活性氧清除作用的过氧化物酶表现出高活性(6.5 unit*min-1*g-1 FW).诱导处理第三天,细胞核DNA呈梯度条带降解,部分细胞出现程序化死亡.内生菌细胞壁寡糖提取物引起的生理反应有利于细胞中青蒿素的生物合成. 相似文献