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801.
Variation in UV sensitivity among common frog Rana temporaria populations along an altitudinal gradient 总被引:1,自引:0,他引:1
Solar ultraviolet-B (UV-B) radiation can be harmful for developing amphibians. As the UV-B dose increases with altitude, it has been suggested that high-altitude populations may have an increased tolerance to high levels of UV-B radiation as compared to lowland populations. We tested this hypothesis with the common frog (Rana temporaria) by comparing populations from nine altitudes (from 333 to 2450m above sea level). Eggs collected in the field were used for laboratory experiments, i.e., exposed to high levels of artificial UV-B radiation. Eggs were reared at 14+/-2 degrees C and exposed to UV treatments until hatching. Embryonic developmental rates increased strongly and linearly with increasing altitude, suggesting a genetic capacity for faster development in highland than lowland eggs. Body length at hatching varied significantly with UV-B treatments, being lower when eggs developed under direct UV-B exposure. Body length at hatching also increased as the altitude of populations increased, but UV-B exposure times were shorter as altitude of population increased. However, the body length difference between exposed and non-exposed individuals in each population decreased as altitude of populations increased, suggesting a costly effect of UV exposure on growth. Type of UV exposure did not influence the mean rates of embryonic mortality and deformity, but both mortality and deformity rates increased as the altitude of populations increased (while UV-B exposure duration decreased). The effect of UV-B on body length at hatching, mortality, and deformities suggests that the sensitivity to UV-B varied among populations along the altitudinal gradient. These results are discussed in evolutionary terms, specifically the potential of R. temporaria high-altitude populations to develop local genetic adaptation to high levels of UV-B. 相似文献
802.
Jaluria P Konstantopoulos K Betenbaugh M Shiloach J 《Biotechnology and bioengineering》2008,99(6):1443-1452
Animal-derived serum is an essential media supplement for mammalian cells in cell culture. For a number of reasons including cost, regulatory concerns, lot inconsistency, potential contamination with adventitious agents, and down-stream processing it is desirable to eliminate the use of serum. Existing protocols designed to adapt cells to serum-free media (SFM) are time-consuming and provide little insight into how the cells adapt. To better understand the physiological responses associated with serum withdrawal and to expedite the adaptation process, a Human Embryonic Kidney-293 (HEK-293) cell line was propagated in 10% fetal bovine serum (FBS) and was progressively adapted to SFM and analyzed at specific serum levels by oligonucleotide microarrays. Of the differentially expressed genes two, early growth response 1 (egr1) and growth arrest specific 6 (gas6), were selected for further analysis based on their level of differential expression, overall expression patterns, and proposed functionalities. HEK-293 cells, propagated in 10% FBS were transfected with egr1 or gas6 and then adapted to SFM. Results indicated that higher expression of either gene moderately enhanced the ability of both cell lines to adapt to SFM. Egr1 appeared to have a greater impact on adaptability than gas6. Results also indicated that specific protein production was unaltered when the expression of egr1 was increased. Flow cytometric analysis revealed increased expression of egr1 was associated with an increase in the percentage of cells in the G2/M phases. These results indicate that enhanced expression of egr1 or gas6 facilitate adaptation to SFM by improving growth and viability. 相似文献
803.
Although many tropical stream consumers have large effects on resource quantity and quality, little is known about the relative importance of consumption versus bioturbation. We quantified egestion rates of freshwater shrimps ( Atya spp. and Xiphocaris elongata ) and mayflies (Leptophlebiidae) in two forest streams within the Luquillo Experimental Forest, Puerto Rico. Consumer body size was a strong predictor of egestion rates, with mass-specific egestion rates declining with body size and per-individual egestion rates increasing with body size. We used these egestion rates, together with published rates of epilithon removal by consumers and epilithon accrual by particle deposition and periphytic growth ( i.e. , with and without consumer access), to estimate the importance of consumption and bioturbation after storm events and during base-flow conditions. Our estimates suggest that direct consumption of epilithon can only account for a minor proportion (< 5%) of material removed following storm events, with most removal likely resulting from bioturbation. During base-flow conditions, we found that consumers (largely shrimps) may be capable of consuming a significant proportion of the material that would otherwise accrue on benthic substrata, but this result was limited to one high-elevation site. Our study suggests that bioturbation is the dominant process that redistributes and entrains fine particles after storm events, and that a variable fraction of deposited and accrued benthic material may be consumed during base-flow conditions. Our results underscore the importance of tropical stream animals in altering the benthic environment through both consumption and bioturbation, and suggest that consumer-mediated material cycling is likely to be context dependent. 相似文献
804.
Global assessment of coral bleaching and required rates of adaptation under climate change 总被引:9,自引:0,他引:9
SIMON D. DONNER WILLIAM J. SKIRVING† CHRISTOPHER M. LITTLE‡ MICHAEL OPPENHEIMER ‡ OVE HOEGH-GULDBERG§ 《Global Change Biology》2005,11(12):2251-2265
Elevated ocean temperatures can cause coral bleaching, the loss of colour from reef‐building corals because of a breakdown of the symbiosis with the dinoflagellate Symbiodinium. Recent studies have warned that global climate change could increase the frequency of coral bleaching and threaten the long‐term viability of coral reefs. These assertions are based on projecting the coarse output from atmosphere–ocean general circulation models (GCMs) to the local conditions around representative coral reefs. Here, we conduct the first comprehensive global assessment of coral bleaching under climate change by adapting the NOAA Coral Reef Watch bleaching prediction method to the output of a low‐ and high‐climate sensitivity GCM. First, we develop and test algorithms for predicting mass coral bleaching with GCM‐resolution sea surface temperatures for thousands of coral reefs, using a global coral reef map and 1985–2002 bleaching prediction data. We then use the algorithms to determine the frequency of coral bleaching and required thermal adaptation by corals and their endosymbionts under two different emissions scenarios. The results indicate that bleaching could become an annual or biannual event for the vast majority of the world's coral reefs in the next 30–50 years without an increase in thermal tolerance of 0.2–1.0°C per decade. The geographic variability in required thermal adaptation found in each model and emissions scenario suggests that coral reefs in some regions, like Micronesia and western Polynesia, may be particularly vulnerable to climate change. Advances in modelling and monitoring will refine the forecast for individual reefs, but this assessment concludes that the global prognosis is unlikely to change without an accelerated effort to stabilize atmospheric greenhouse gas concentrations. 相似文献
805.
Climate change will ultimately affect the supply and quality of freshwater lakes and rivers throughout the world. This study examines the potential impacts of climate change on freshwater fish distributions in Canada. Climate normals data (means from 1961 to 1990) from Environment Canada were used to map current climate found throughout the tertiary watersheds of Canada. Logistic regressions based on these climate data were used to develop predictive presence‐absence equations for (a) common commercially and recreationally important species and (b) an Arctic freshwater species and a freshwater fish species of conservation significance listed by the Committee on the Status of Endangered Wildlife (COSEWIC). The Canadian Centre for Climate Modelling and Analysis Global Coupled Model 2(IS92a) provided forecasts of Canada's climate in 2020 and 2050. The data from this scenario and the logistic regressions provided a ready framework for predicting the potential distributions of the fishes. Physical and ecological barriers would have to be overcome for the distribution of these species to actually change in response to climate change. Generally, coldwater species may be extirpated from much of their present range while cool and warm‐water species may expand northward. Species that are limited to the most southern regions of the country may expand northwards. A conceptual framework for assessing potential climate change impacts on fishes and the variety of management strategies required to deal with these impacts are discussed. Our forecasts demonstrate the need for climate change assessments in species at risk as well as for common species. 相似文献
806.
遮荫处理下多脉青冈和金叶含笑的叶解剖结构变化研究 总被引:7,自引:1,他引:6
运用结构植物学方法,以多脉青冈(Cyclobalanopsis multinervis)和金叶含笑(Michelia foveolata)2年生苗为材料,进行全光照处理和遮荫处理下叶结构观察比较。结果显示,长期遮荫处理下,多脉青冈和金叶含笑新生叶的表皮细胞变薄,栅栏组织变得稀疏,层数有减少趋势,海绵组织更加疏松;多脉青冈的叶角质层退化。表明长期的弱光照下,2种树种的新生叶叶结构产生了适廊性的变化,其适应程度可以推测植物对光环境变化的适应能力强弱和进化潜力,研究结果对预测园林树种的光适应能力和结构进化有一定意义。 相似文献
807.
B. B. Vartapetian 《Russian Journal of Plant Physiology》2005,52(6):826-844
This review attempted to follow the establishment of a novel branch of biology arisen at the interfaces between plant physiology, biochemistry, and molecular biology—plant anaerobic stress. Most attention was given to the early period of these investigations, the activity of the members of International Society for Plant Anaerobiosis in particular, and the contribution of Russian scientists, who played a significant role at that time in the establishment and international recognition of this new trend. In this connection, the following points are considered: (1) Crawford's metabolic theory, which could not withstand experimental verification but induced an active discussion, thus stimulating further investigations in this field; (2) a concept of two main strategies of plant adaptation to anaerobic stress (true and apparent adaptation), which was put forward based on the following experimental data: (a) a discovery of a paradoxical phenomenon of hyper-sensitivity, but not hyper-resistance to anoxia, of the flood-tolerant plant roots (“apparent” tolerance); (b) the elucidation of the physiological role of oxygen transported from aerated organs of flood-tolerant plants to the roots inhabiting anaerobic environment; (c) demonstration of the key role of both energy metabolism, and (d) substrate providing for glycolysis and ethanolic fermentation in plants manifesting “true” tolerance to oxygen deprivation; (3) the discovery of plant stress proteins; and finally (4) pH-stat theory put forward by Davies. 相似文献
808.
Nadia Aarab Olivier Champeau Pascal Mora Mich le Daubeze Philippe Garrigues Jean-Fran ois Narbonne 《Biomarkers》2004,9(3):258-270
The aim was to apply a multimarker scoring approach as complementary to freshwater monitoring programmes carried out by the Water Agency Adour-Garonne. Fish (chub, barbel and trout) were collected in 11 sites in rivers in south-west France. Five biomarkers of response were measured either in muscle or brain for acetylcholinesterase (AChE) and in liver for glutathione S-transferase, catalase and 7-ethoxyresorufine O-deethylase. As a result of multivariate analysis, sites were clearly discriminated mainly by 7-ethoxyresorufine O-deethylase and acetylcholinesterase activities. According to the scoring approach, a multimarker pollution index was calculated for each sampling site as the sum of the response index of the five measured biomarkers (pollution index). Sorting was established by ranging the sites from lightly to highly contaminated locations. 相似文献
809.
Ionut Cardei Srivatsan Varadarajan Allalaghatta Pavan Lee Graba Mihaela Cardei Manki Min 《Cluster computing》2004,7(1):91-103
Boosted by technology advancements, government and commercial interest, ad-hoc wireless networks are emerging as a serious platform for distributed mission-critical applications. Guaranteeing QoS in this environment is a hard problem because several applications may share the same resources in the network, and mobile ad-hoc wireless networks (MANETs) typically exhibit high variability in network topology and communication quality. In this paper we introduce DYNAMIQUE, a resource management infrastructure for MANETs. We present a resource model for multi-application admission control that optimizes the application admission utility, defined as a combination of the QoS satisfaction ratio. A method based on external adaptation (shrinking QoS for existing applications and later QoS expansion) is introduced as a way to reduce computation complexity by reducing the search space. We designed an application admission protocol that uses a greedy heuristic to improve application utility. For this, the admission control considers network topology information from the routing layer. Specifically, the admission protocol takes benefit from a cluster network organization, as defined by ad-hoc routing protocols such as CBRP and LANMAR. Information on cluster membership and cluster head elections allows the admission protocol to minimize control signaling and to improve application quality by localizing task mapping. 相似文献
810.
Promising directions in plant phenotypic plasticity 总被引:9,自引:0,他引:9
A research agenda for the next phase of plasticity studies calls for contributions from a diverse group of biologists, working both independently and collaboratively, to pursue four promising directions: examining dynamic, anatomical/architectural, and cross-generational plasticity along with simpler growth traits; carefully assessing the adaptive significance of those plasticity patterns; investigating the intricate transduction pathways that lead from environmental signal to phenotypic response; and considering the rich environmental context of natural systems. Progress in these areas will allow us to address broad and timely questions regarding the ecological and evolutionary significance of plasticity and the nature of phenotypic determination. 相似文献