全文获取类型
收费全文 | 940篇 |
免费 | 37篇 |
国内免费 | 56篇 |
出版年
2024年 | 4篇 |
2023年 | 10篇 |
2022年 | 19篇 |
2021年 | 14篇 |
2020年 | 15篇 |
2019年 | 19篇 |
2018年 | 20篇 |
2017年 | 13篇 |
2016年 | 29篇 |
2015年 | 29篇 |
2014年 | 28篇 |
2013年 | 76篇 |
2012年 | 28篇 |
2011年 | 23篇 |
2010年 | 28篇 |
2009年 | 43篇 |
2008年 | 31篇 |
2007年 | 49篇 |
2006年 | 37篇 |
2005年 | 47篇 |
2004年 | 38篇 |
2003年 | 31篇 |
2002年 | 27篇 |
2001年 | 23篇 |
2000年 | 31篇 |
1999年 | 25篇 |
1998年 | 21篇 |
1997年 | 9篇 |
1996年 | 23篇 |
1995年 | 16篇 |
1994年 | 12篇 |
1993年 | 11篇 |
1992年 | 14篇 |
1991年 | 13篇 |
1990年 | 10篇 |
1989年 | 7篇 |
1988年 | 4篇 |
1987年 | 8篇 |
1986年 | 12篇 |
1985年 | 18篇 |
1984年 | 14篇 |
1983年 | 12篇 |
1982年 | 14篇 |
1981年 | 17篇 |
1980年 | 9篇 |
1979年 | 12篇 |
1978年 | 8篇 |
1977年 | 5篇 |
1975年 | 7篇 |
1973年 | 10篇 |
排序方式: 共有1033条查询结果,搜索用时 15 毫秒
71.
C.C. Appleton R.C. Krecek A. Verster M.R. Bruorton M.J. Lawes 《Journal of medical primatology》1994,23(1):52-55
Eight gastro-intestinal tracts of Cercopithecus mitis labiatus from Karkloof, Natal, and 121 fecal samples from C. m. erythrarchus from Cape Vidal, Natal, were examined for helminth parasites and/or their eggs. Fecal samples from six of the C. m. labiatus were examined for protozoan cysts. Five protozoon and six helminth species were identified from C.m labiatus. Most adult worms occurred in the caecum and colon, gut regions which also contained the highest volatile fatty acid levels. The eggs of nine helminth species were recovered from C. m. erythrarchus fecal samples; protozoans were not looked for in these samples. 相似文献
72.
Aims: To determine the potential of the plant‐parasitic nematode Meloidogyne javanica to serve as a temporary reservoir for Escherichia coli. Methods and Results: The adhesion to and persistence of E. coli on the surface of M. javanica were evaluated at different times and temperatures. A pure culture of green fluorescent protein (GFP) tagged E. coli was mixed with ca. 1000 J2 M. javanica for 2 h at 25°C. The nematodes were then washed and the rate of the adhesion of the bacteria to the nematodes was determined by counting the viable nematode‐associated E. coli, and by fluorescence microscopy. A dose‐dependent adhesion rate was observed only at a bacterium to nematode ratio of 104–106 : 1. The adhesion of E. coli to the nematodes was also tested over a 24 h‐period at 4°C, 25°C and 37°C. At 4°C and 37°C, maximal adhesion was observed at 5 h; whereas at 25°C, maximal adherence was observed at 8 h. Survival experiments showed that the bacteria could be detected on the nematodes for up to 2 weeks when incubated at 4°C and 25°C, but not at 37°C. Conclusions: Under laboratory conditions, at 4°C and 25°C, M. javanica could serve as a temporary vector for E. coli for up to 2 weeks. Significance and Impact of the Study: These findings support the hypothesis that, in the presence of high concentrations of E. coli, M. javanica might serve as a potential vehicle for the transmission of food‐borne pathogens. 相似文献
73.
74.
75.
Previously, the combination of reduced rate of entomopathogenic nematodes (EPN) and fungus caused additive or synergistic mortality to third-instar black vine weevil (BVW), Otiorhynchus sulcatus. In this study, we examined this interaction in unheated glasshouses during winter and compared a combination of commercial formulation of a cold-tolerant EPN, S. kraussei (Nemasys L?) and fungus Metarhizium anisopliae strain V275 against overwintering third-instar BVW. The combination of M. anisopliae with S. kraussei at a rate of 1×1010 conidia+250,000 nematodes/growbag resulted in additive or synergistic effects, providing 100% control of overwintering larvae. 相似文献
76.
Williams AR Greeff JC Vercoe PE Dobson RJ Karlsson LJ 《Animal : an international journal of animal bioscience》2010,4(1):122-127
The peri-parturient period is crucial for controlling worms as the acquired immunity of ewes is disrupted, resulting in an increase in faecal worm egg counts. Two hypotheses were tested in this experiment - that ewes bred for worm resistance would have lower faecal worm egg counts than unselected control ewes, during late pregnancy and lactation, under similar but separate grazing areas; and also that numbers of infective nematode larvae would be lower on pastures grazed by resistant ewes than pastures grazed by unselected control ewes. Faecal samples were collected from resistant and unselected ewes in late pregnancy and early lactation, during the winter rainfall season, and analysed for numbers of Trichostrongylus colubriformis and Teladorsagia circumcincta. Pasture samples were taken 1 week before and 7 weeks after lambing started and analysed for infective larvae. In all sheep, worm egg counts rose 2 weeks prior to lambing and continued into lactation. Worm egg counts were significantly lower in the resistant ewes from 1 week before lambing to 2 weeks after lambing. There were no differences in egg counts between single- and twin-bearing ewes in the resistant line. However, twin-bearing control ewes had significantly higher egg counts than single-bearing control ewes. Following lactation, plots grazed by resistant ewes had substantially less contamination with T. colubriformis larvae, but there were no differences in numbers of T. circumcincta larvae. Our results demonstrate that sheep bred for worm resistance has lower worm burdens during the peri-parturient phase and that lambs born to resistant ewes face a lower larval challenge during their introduction to grazing. In our environment, selection for low worm egg counts has produced sheep highly resistant to T. colubriformis, but has had less impact on resistance towards T. circumcincta. 相似文献
77.
At high dosages, infective-stage juveniles of Neoaplectana carpocapsae and Heterorhabditis heliothidis were able to penetrate into and kill the garden millipede. Nematode development was inhibited by host defense reactions which consisted of encapsulation and enmeshment in tracheoles and connective tissue surrounding the millipede's midgut. Foreign bacteria quickly entered the millipede's hemocoel at death and out-competed the nematode's symbiotic bacteria, Xenorhabdus sp. The dauer stages of an unidentified rhabditid nematode were associated with the living millipede. These nematodes reproduced on the millipede's cadaver. 相似文献
78.
The accumulation of faeces in the wool of the breech area (tail, perineum and anus) of lambs, known as faecal soiling, has been shown to be one of the major factors predisposing sheep to blowfly strike. However, the causes of faecal soiling of lambs in the UK are not clearly understood. Hence, in this investigation, the relationships between faecal soiling, gastrointestinal parasitic nematode infection and resultant diarrhoea were examined in a longitudinal study of 200 lambs at two farms in south-west England. Faecal egg counts, pasture worm burdens, faecal soiling and growth rates were recorded for individually tagged lambs over the summer of 2003. Grass growth and nutritional composition (protein and fibre) and weather data were also recorded over this period. Analysis using linear mixed models showed that faecal soiling was associated with higher strongyle-type egg counts, longer periods since worming, lower live-weights, female gender, lower faecal consistency and pasture quality. The data indicate that dag scoring, especially in mid- to late summer, could be used as a rapid, non-invasive technique for selecting animals, particularly lambs, with high faecal egg counts for selective drenching to reduce the incidence of anthelmintic resistance. Selective drenching of lambs with high dag scores would also be expected to aid in the control of blowfly strike. 相似文献
79.
Habitat associations of two entomopathogenic nematodes: a quantitative study using real-time quantitative polymerase chain reactions 总被引:1,自引:0,他引:1
1. Despite nematodes being the most abundant animals on earth, very few animal ecologists study them, probably because of the difficulties of identifying them to species by morphological methods. 2. A group of nematodes that are important both ecologically and economically is the entomopathogenic nematodes, which play a key role in regulating soil food webs and are sold throughout the world as biological insecticides, yet for which very little is known of their population ecology. 3. A novel detection and quantification method was developed for soil nematodes using real-time polymerase chain reaction (PCR), and the technique was used to estimate numbers of two closely related species of entomopathogenic nematodes, Steinernema kraussei and S. affine in 50 soil samples from 10 sites in Scotland representing two distinct habitats (woodland and grassland). 4. There was a high degree of correlation between our molecular and traditional morphological estimates of population size and our data clearly showed that Steinernema affine occurred only in grassland areas, whereas S. kraussei was found in grassland and woodland samples to a similar degree. 5. Real-time PCR offers a rapid and accurate method of detecting individual nematode species from soil samples without the need for a specialist taxonomist, and has much potential for use in studies of nematode population ecology. 相似文献
80.
Anderson S. Dias Jackson V. Araújo Artur K. Campos Fabio R. Braga Thiago A. Fonseca 《World journal of microbiology & biotechnology》2007,23(9):1245-1252
The viability of a formulation of Duddingtonia flagrans was assessed in the control of parasite gastrointestinal nematodes of cattle. Two groups (A and B) of eight crossbred Holstein × Zebu
cattle, approximately one year old, were placed in Brachiaria decumbens pasture. Each animal in group B (treated) received orally 20 g sodium alginate pellets containing mycelial mass of the D. flagrans fungus, while the animals in the group A (control) received pellets without fungus for seven months, starting in March 2005.
The egg per gram of feces counting the gastrointestinal nematodes showed a difference (P < 0.05) in the treated group in June, July and August, with reductions of 58% (June), 47% (July) and 51% (August) compared
to the control group. The infective larvae recovered in the pastures collected up to 20 cm from distance of the fecal dung
in group B differed (P < 0.01) from the larvae recovered in group A. At the end of the experimental period, the animals in group B presented a greater
weight gain (P < 0.01) compared to the untreated group (A). The treatment of cattle with pellets containing the D. flagrans nematophagous fungus, at the dose and duration used was effective in controlling the infective larvae of gastrointestinal
nematodes of cattle. 相似文献