全文获取类型
收费全文 | 43028篇 |
免费 | 3271篇 |
国内免费 | 5464篇 |
出版年
2024年 | 169篇 |
2023年 | 876篇 |
2022年 | 1110篇 |
2021年 | 1440篇 |
2020年 | 1470篇 |
2019年 | 2000篇 |
2018年 | 1579篇 |
2017年 | 1509篇 |
2016年 | 1432篇 |
2015年 | 1384篇 |
2014年 | 2108篇 |
2013年 | 3006篇 |
2012年 | 1715篇 |
2011年 | 1928篇 |
2010年 | 1550篇 |
2009年 | 1988篇 |
2008年 | 2008篇 |
2007年 | 2171篇 |
2006年 | 1925篇 |
2005年 | 1748篇 |
2004年 | 1487篇 |
2003年 | 1406篇 |
2002年 | 1336篇 |
2001年 | 1047篇 |
2000年 | 960篇 |
1999年 | 872篇 |
1998年 | 835篇 |
1997年 | 729篇 |
1996年 | 634篇 |
1995年 | 620篇 |
1994年 | 629篇 |
1993年 | 533篇 |
1992年 | 521篇 |
1991年 | 535篇 |
1990年 | 417篇 |
1989年 | 380篇 |
1988年 | 386篇 |
1987年 | 327篇 |
1986年 | 352篇 |
1985年 | 565篇 |
1984年 | 636篇 |
1983年 | 347篇 |
1982年 | 510篇 |
1981年 | 480篇 |
1980年 | 431篇 |
1979年 | 309篇 |
1978年 | 232篇 |
1977年 | 251篇 |
1976年 | 218篇 |
1973年 | 185篇 |
排序方式: 共有10000条查询结果,搜索用时 250 毫秒
951.
N-甲基甲酰胺碱度是提取高质量固氮酶铁钼辅基的关键因素之一。过量的亚甲蓝能氧化并分解铁铜铺基为含双相铁硫簇和铁硫簇固氮酶铁钼辅基和在紫外可见光谱区中均无特征吸收峰,而在320nm处却呈弱吸收峰,棕色固氮菌固氮酶和该菌的突变菌侏UW45固氮酶(缺铁钼辅基)中的非含钼的铁硫簇在紫外可见光谱区320nm和405nm处均含有特征吸收峰. 相似文献
952.
经过硫酸铵30%~50%分级沉淀、二步柱层析可获聚丙烯酰胺凝胶电泳均一的粘质赛氏菌胞外蛋白酶制品,收率可达53%,并制备了酶的结晶,该酶以SephadexG100柱层析及SDS-PAGE测得分子量约为81000,该酶的最适pH为7.0,最适温度为45℃,Zn2+、Mn2+、Fe2+、Cu2+、Co2+等重金属离子不同程度地抑制酶活性。 相似文献
953.
Michiro Fujihara 《植被学杂志》1996,7(5):729-738
Abstract. The development of secondary Pinus densiflora (Japanese red pine) forests after pine wilt disease was studied through phytosociological analysis, estimation of forest structure before disease and size-structure, tree ring and stem analyses. Following the end of the disease, the growth of previously suppressed small oak trees was accelerated. This is quite different from the development of forests following fire, which starts with the establishment of pine seedlings. Pine wilt disease shifted the dominance of secondary forests from Pinus densiflora to Quercus serrata oak forest. In pine forests, disturbance by fire is important for forest maintenance. In contrast, disturbance by pine wilt disease leads to an acceleration of succession from pine forest to oak forest. 相似文献
954.
Akio Mori 《Primates; journal of primatology》1995,36(1):11-26
The feeding process of Japanese monkeys on soy beans which were scattered over a sandy beach on Koshima islet was studied.
Younger monkeys were able to pick up more beans when 8 kg of beans were divided and given two times (“two times feeding”)
than when the whole amount (8 kg) of beans was given at one time (“one time feeding”). The effect of saturation of the food
intake capacity in younger monkeys at the first feeding in “two times feeding” did not appear at the second feeding one hour
later. The minutely intake of soy beans (feeding speed) for each age class was analyzed. The decline of feeding speed in adult
females after the peak in “one time feeding” was not related to the decline in density of beans on the ground, and this decline
was caused by saturation of their food intake capacity.
Adult females were divided into four classes according to their dominance rank order: high, lower-high, higher-low, and low
classes. The total amount of intake in “one time feeding” was far larger in the high class than in any of the other classes.
The total amount of feeding in the first feeding of “two times feeding” increased in accordance with rise in the dominance
rank class, and there was no relation to rank and total feeding amount in the second feeding of “two times feeding.” Differences
existed in the process of feeding between the rank classes. The feeding speed of the low class was as high as that of the
high class on the curve of minutely intake, while the low class stopped feeding much earlier than the high class. The lower-high
class displayed a low feeding speed, and stopped feeding the latest. The order of the duration to stay and to feed in the
feeding area was lower-high > high > higher-low > low, and this order did not change under the three different feeding conditions,
“one time feeding,” and the first and second feedings of “two times feeding.” Adolescent females tended to stay the longest
duration in the feeding area among all age classes. Both the lower-high class females and adolescent females had an unstable
social status in the Koshima group, and their social status affected their feeding behaviors. The feeding behaviors were similar
in attitude depending on social status, and are considered to be maintained for a fairly long time.
The feeding strategy of the lower-high class, in staying a longer duration in the artificial feeding area, and departing later,
may be effective under the artificial feeding conditions, but it may be a bad strategy in a natural habitat where the food
is not so clumped as in artificial feeding, and where choice of other food patches is possible. The above results agree well
with previous reports for the Koshima group, indicating that the rank of the lower-high class females was unstable (Mori et al., 1989), and that their reproductive success was low (Watanabe et al., 1992). 相似文献
955.
The three-dimensional pattern of phloem and xylem in 10-d-to two-month-old tumors induced by Agrobacterium tumefaciens (C58) and in adjacent Ricinus communis L. stem tissues was studied in thick sections by clearing with lactic acid and by staining with lacmoid. The crown galls contained two types of vascular strands: treelike branched bundles, which developed towards the tumor surface in fast-growing regions, and globular bundles in the slowly developing parts. Both types of vascular bundles contained xylem and phloem and were continuous with the vascular system of the host plant. The tumor bundles were interconnected by a dense net of phloem anastomoses, consisting of sieve tubes but no vessels. These vascular patterns reflect the apparent synthesis sites, concentration gradients and flow pathways of the plant hormones additionally produced in the tumors upon expression of the T-DNA-encoded genes. The A. tumefaciens-induced crown gall affected vascular differentiation in the host stem. In the basipetal direction, the tumor induced more xylem differentiation directly below it, where the crown-gall bundles joined the vascular system of the host. In the centripetal direction, the crown gall caused the development of pathologic xylem characterized by narrow vessels, giant rays and absence of fibers. On the other hand, most probably as a consequence of its gibberellic acid content, the host plant stimulated a local differentiation of regenerative phloem and xylem fibers with unique ramifications, only at the base of the tumor. However, fibers were absent from the main body of the crown gall. The study shows that A. tumefaciens-induced crown galls are characterized by a sophisticated network of vascular tissues in the tumor and are accompanied by a perturbated vessel system in the host. The hormonal mechanisms controlling vascular differentiation in the tumor and neighboring host tissues are discussed. In addition, the gall constriction hypothesis is proposed for explaining the mechanism which gives priority in water supply to the growing gall over the host shoot.We thank Dr. Zs. Koncz (Max-Planck-Institut für Züchtungsforschung, Köln, Germany) for Agrobacterium strains and the Deutsche Forschungsgemeinschaft (SFB 199) for financial support to C.I.U. 相似文献
956.
The gibberellin (GA)-biosynthesis mutations, lh
i
, ls and Ie
5839
have been used to investigate the role(s) of the GAs in seed development of the garden pea (Pisum sativum L.). Seeds homozygous for lh
i
possess reduced GA levels, are more likely to abort during development, and weigh less at harvest, compared with wild-type seeds due to expression of the lh
i
mutation in the embryo and/ or endosperm. Compared with wild-type seeds, the lh
i
mutation reduces endogenous GA1 and gibberellic acid (GA3) levels in the embryo/endosperm a few days after anthesis and fertilizing lh
i
plants with wild-type pollen dramatically increases GA1 and GA3 levels in the embryo/ endosperm and restores normal seed development. By contrast, the ls and le
5839
mutations do not appear to reduce GA levels in the embryo/endosperm of seeds a few days after anthesis, and do not affect embryo or endosperm development. However, both the ls and lh
i
mutations substantially reduce endogenous GA levels in embryos at contact point (the first day the liquid endosperm disappears). Levels of GAs in seeds from crosses involving the ls and lh
i
mutations suggest that GAs are synthesised in both the embryo/endosperm and testa and that the expression of ls depends on the tissue and developmental stage examined. These results suggest that GAs (possibly GA1 and/or GA3) play an important role early in pea seed development by regulating the development of the embryo and/or endosperm. By contrast, the high GA levels found in wild-type seeds at contact point (and beyond) do not appear to have a physiological role in seed development.Abbreviations GAn
gibberellin An
- DAA
days after anthesis
- WT
wild-type
We thank Noel Davies, Katherine McPherson and Peter Bobbi for technical assistance, Professor L. Mander (ANU, Canberra) for dideuterated GA standards, and the Australian Research Council and Frontier Research Program, The Institute of Physical and Chemical Research (RIKEN, Japan), for financial support. 相似文献
957.
A. Abo-elwafa K. Murai T. Shimada 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1995,90(3-4):335-340
Randomly amplified polymorphic DNA (RAPD) markers were used to estimate intra- and interspecific variations in the genus Lens (lentil). Twenty cultivars of L. culinaris ssp. culinaris, including 11 microsperma (small-seeded) and nine macrosperma (large-seeded) types, and 16 wild relatives (four accessions each of L. culinaris ssp. orientalis, L. odemensis, L. nigricans and L. ervoides), were evaluated for genetic variability using a set of 40 random 10-mer primers. Fifty reproducibly scorable DNA bands were observed from ten of the primers, 90% of which were polymorphic. Genetic distances between each of the accessions were calculated from simple matching coefficients. A dendrogram showing genetic relationships between them was constructed by an unweighted pair-group method with arithmetical averages (UPGMA). This study revealed that (1) expect for L. ervoides, the level of intraspecific variation in cultivated lentil is lower than that in wild species, (2) L. culinaris ssp. orientalis is the most likely candidate for a progenitor of the cultivated species, and (3) microsperma and macrosperma cultivars were indistinguishable by the RAPD markers identified here. 相似文献
958.
A non-equilibrium thermodynamic theory of generalized Lotka-Volterra ecosystem has been presented. The main results consist of the derivation of a generalized expression of entropy-production for the evolutionary ecosystem and the study of its role in the analysis of ecological stability, succession and also in the formulation of some extremum principles characterising the evolution of the ecosystem. 相似文献
959.
F. R. Eirich 《Journal of biological physics》1995,20(1-4):349-358
Believing, for good reasons, that our Universe engenders the occurrence of Life, it is probable that Life appeared more than once on young planet Earth. The universality of our genetic code indicates though, that only one line produced a population that was cellular, autopoietic, responsive intelligently (i.e., to its advantage) to its environment, and capable of reproduction. Its evolution took time during which other intermediate or incipient forms of life disappeared. Trying to retrace ab initio the preceding chemical evolution on our young planet, we may not repeat the chemistry which led to our line. One can argue that it may be profitable to attempt to construct artificial life forms using the kind of building blocks that we learned to know through studying the chemistry of our life. Independently, computers are being built, or are planned, which possess memory that can be used appropriately (intelligently), and which to some extent can repair and reproduce themselves. Such Life does not depend on our biochemistry. However, these machines do perform as yet only partly along the lines of our human intelligence which is guided by self-awareness and a sense of individual integrity, and are thereby not equipped to search for Understanding and Self-expression. 相似文献
960.
Magdalena J. Rossowska Parvaneh Ghanel Tetsuo Nakamoto 《Biological trace element research》1995,50(3):229-236
The purpose of this study was to determine the relationship between concentrations of Zn and Cu and the activities of superoxide
dismutase and glutathione peroxidase in the heart and liver of young rat pups whose dams were fed a diet supplemented with
caffeine and/or Zn. Four groups of dams with their newborn pups were fed one of the following diets for 22 d: 20% protein
basal diet; the basal diet supplemented with caffeine (2 mg/100 body wt); the basal diet supplemented with Zn (300 mg/kg diet);
or the basal diet supplemented with caffeine plus Zn. The Cu levels in the livers of the pups were decreased by maternal intake
of the caffeine and Zn diet. The maternal intake of the caffeine diet increased Mn-superoxide dismutase (MnSOD) activity and
Cu, Zn-superoxide dismutase (CUZnSOD) in the heart of the pups. On the other hand, the activity of Cu,ZnSOD was significantly
reduced in the liver of pups whose dams consumed a caffeine, Zn, or caffeine plus Zn diet. Cu, ZnSOD activity in the liver
of the pups seems to be correlated with Cu levels in the tissue. Selenium-dependent glutathione peroxidase (GSH-Px) activities
in the heart and liver showed no difference among the groups. The effect of dietary caffeine and/or Zn on the activity of
antioxidant enzymes in the heart and liver were different in young rats. The activities of these enzymes in the heart were
lower than in the liver of 22-d-old rats. Our experiments indicate that the heart has limited defenses against the toxic effects
of peroxides when compared to the liver. 相似文献