全文获取类型
收费全文 | 9239篇 |
免费 | 498篇 |
国内免费 | 328篇 |
专业分类
10065篇 |
出版年
2024年 | 65篇 |
2023年 | 442篇 |
2022年 | 318篇 |
2021年 | 417篇 |
2020年 | 538篇 |
2019年 | 644篇 |
2018年 | 570篇 |
2017年 | 497篇 |
2016年 | 533篇 |
2015年 | 391篇 |
2014年 | 658篇 |
2013年 | 1231篇 |
2012年 | 206篇 |
2011年 | 232篇 |
2010年 | 204篇 |
2009年 | 208篇 |
2008年 | 239篇 |
2007年 | 250篇 |
2006年 | 222篇 |
2005年 | 248篇 |
2004年 | 189篇 |
2003年 | 169篇 |
2002年 | 196篇 |
2001年 | 150篇 |
2000年 | 141篇 |
1999年 | 106篇 |
1998年 | 98篇 |
1997年 | 86篇 |
1996年 | 64篇 |
1995年 | 66篇 |
1994年 | 87篇 |
1993年 | 47篇 |
1992年 | 63篇 |
1991年 | 54篇 |
1990年 | 39篇 |
1989年 | 35篇 |
1988年 | 21篇 |
1987年 | 27篇 |
1986年 | 21篇 |
1985年 | 48篇 |
1984年 | 43篇 |
1983年 | 36篇 |
1982年 | 33篇 |
1981年 | 24篇 |
1980年 | 26篇 |
1979年 | 17篇 |
1978年 | 16篇 |
1977年 | 24篇 |
1975年 | 8篇 |
1974年 | 6篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
61.
Hettinga PN Arnason AN Manseau M Cross D Whaley K Wilson PJ 《The Journal of wildlife management》2012,76(6):1153-1164
A critical step in recovery efforts for endangered and threatened species is the monitoring of population demographic parameters. As part of these efforts, we evaluated the use of fecal-DNA based capture–recapture methods to estimate population sizes and population rate of change for the North Interlake woodland caribou herd (Rangifer tarandus caribou), Manitoba, Canada. This herd is part of the boreal population of woodland caribou, listed as threatened under the federal Species at Risk Act (2003) and the provincial Manitoba Endangered Species Act (2006). Between 2004 and 2009 (9 surveys), we collected 1,080 fecal samples and identified 180 unique genotypes (102 females and 78 males). We used a robust design survey plan with 2 surveys in most years and analysed the data with Program MARK to estimate encounter rates (p), apparent survival rates (φ), rates of population change (λ), and population sizes (N). We estimated these demographic parameters for males and females and for 2 genetic clusters within the North Interlake. The population size estimates were larger for the Lower than the Upper North Interlake area and the proportion of males was lower in the Lower (33%) than the Upper North Interlake (49%). Population rate of change for the entire North Interlake area (2005–2009) using the robust design Pradel model was significantly <1.0 (λ = 0.90, 95% CI: 0.82–0.99) and varied between sex and area with the highest being for males in Lower North Interlake (λ = 0.98, 95% CI: 0.83–1.13) and the lowest being for females in Upper North Interlake (λ = 0.83, 95% CI: 0.69–0.97). The additivity of λ between sex and area is supported on the log scale and translates into males having a λ that is 0.09 greater than females and independent of sex, Lower North Interlake having a λ that is 0.06 greater than Upper North Interlake. Population estimates paralleled these declining trends, which correspond to trends observed in other fragmented populations of woodland caribou along the southern part of their range. The results of this study clearly demonstrate the applicability and success of non-invasive genetic sampling in monitoring populations of woodland caribou. © 2012 The Wildlife Society. 相似文献
62.
Primates tend to prefer specific plant foods, and primate home ranges may contain only a subset of food species present in an area. Thus, primate feeding strategies should be sensitive to the phenology of specific species encountered within the home range in addition to responding to larger scale phenomena such as seasonal changes in rainfall or temperature. We studied three groups of Javan gibbons (Hylobates moloch) in the Gunung Halimun‐Salak National Park, Indonesia from April 2008 to March 2009 and used general linear mixed models (GLMM) and a model selection procedure to investigate the effects of variation in fruit and flower availability on gibbon behavior. Preferred foods were defined as foods that are overselected relative to their abundance, while important food species were those that comprised >5% of feeding time. All important species were also preferred. Season and measurements of flower and fruit availability affected fruit‐feeding time, daily path lengths (DPL), and dietary breadth. Models that included the availability of preferred foods as independent variables generally showed better explanatory power than models that used overall fruit or flower availability. For one group, fruit and preferred fruit abundance had the strongest effects on diets and DPL in the models selected, while another group was more responsive to changes in flower availability. Temporal variation in plant part consumption was not correlated in neighboring groups. Our results suggest that fine‐scale local factors are important determinants of gibbon foraging strategies. Am. J. Primatol. 74:1154‐1167, 2012. © 2012 Wiley Periodicals, Inc. 相似文献
63.
Giovanna Collyer Daniel M. Perkins Danielle K. Petsch Tadeu Siqueira Victor Saito 《Global Change Biology》2023,29(14):4094-4106
Land-use and land-cover transitions can affect biodiversity and ecosystem functioning in a myriad of ways, including how energy is transferred within food-webs. Size spectra (i.e. relationships between body size and biomass or abundance) provide a means to assess how food-webs respond to environmental stressors by depicting how energy is transferred from small to larger organisms. Here, we investigated changes in the size spectrum of aquatic macroinvertebrates along a broad land-use intensification gradient (from Atlantic Forest to mechanized agriculture) in 30 Brazilian streams. We expected to find a steeper size spectrum slope and lower total biomass in more disturbed streams due to higher energetic expenditure in physiologically stressful conditions, which has a disproportionate impact on large individuals. As expected, we found that more disturbed streams had fewer small organisms than pristine forest streams, but, surprisingly, they had shallower size spectrum slopes, which indicates that energy might be transferred more efficiently in disturbed streams. Disturbed streams were also less taxonomically diverse, suggesting that the potentially higher energy transfer in these webs might be channelled via a few efficient trophic links. However, because total biomass was higher in pristine streams, these sites still supported a greater number of larger organisms and longer food chains (i.e. larger size range). Our results indicate that land-use intensification decreases ecosystem stability and enhances vulnerability to population extinctions by reducing the possible energetic pathways while enhancing efficiency between the remaining food-web linkages. Our study represents a step forward in understanding how land-use intensification affects trophic interactions and ecosystem functioning in aquatic systems. 相似文献
64.
Although the clade Crocodylomorpha is represented by few extant species (Crocodylia), it has a rich fossil record. Hundreds of species, adapted to terrestrial, semi-aquatic and marine environments, have existed over more than 200 million years. Numerous studies have attempted to characterize the factors driving the diversification and extinction events of Crocodylomorpha, resulting in ambiguous and even contradictory conclusions, which points to the need for phylogenetically and temporally smaller-scaled studies. Here, we focus on differential survival at the Cretaceous–Palaeogene (K–Pg) crisis of Notosuchia, a diverse clade of mostly terrestrial Crocodylomorpha that achieved great diversity during the Cretaceous. More precisely, we tested the effect of body size and palaeotemperatures on notosuchian survival probability during the K–Pg crisis as well as the effect of diet on the evolution of their body size. We find that Notosuchia showed an evolutionary trend towards larger body sizes through time, associated with a shift from an omnivorous to a carnivorous diet. This may explain why sebecids were the only notosuchians to survive the K–Pg crisis. We also corroborate the conclusions of previous studies that detected a Lagerstätten effect occurring in the Adamantina Formation (Upper Cretaceous, Brazil, Bauru Group). This work confirms the value of more finely-scaled macroevolutionary studies for understanding the history of a rich and complex group such as Crocodylomorpha. 相似文献
65.
Midelet-Bourdin G Leleu G Copin S Roche SM Velge P Malle P 《Journal of applied microbiology》2006,101(2):300-308
AIM: To assess the effect of different foods, which have been implicated or not in cases of listeriosis, on the in vitro virulence-associated phenotype level of different Listeria monocytogenes strains. METHODS AND RESULTS: The virulence-associated phenotype level of L. monocytogenes was studied with the in vitro cell test based on a plaque-forming assay with a human adenocarcinoma cell line (HT-29) monolayer. Three strains of L. monocytogenes were grown in preparations (homogenate, 1-mum filtrate or 0.2-mum filtrate) of different food extracts ['rillettes' (potted minced pork), milk, raw salmon and cold-smoked salmon] or in a control medium, brain heart infusion (BHI). The bacterial suspensions grown in food extracts or in BHI at 37 degrees C were diluted with their growth medium (food extract or BHI) or with minimum essential medium before seeding on confluent HT-29 cell monolayers. Filtration of food extracts had no significant effect on the plaque numbers formed by the bacteria. A significant decrease in the plaque numbers was noted for the three strains when they grew in the rillettes extracts, compared with the other food extracts and BHI. The levels of in vitro virulence-associated phenotype of the strains after growth in the rillettes extract were similar to or lower than that of the hypovirulent internal reference strain L. monocytogenes 442. After growth in milk and cold-smoked salmon, the impact on virulence-associated phenotype depended on the strain. In contrast, plaque-forming assay indicated increased virulence-associated phenotype when the strains were switched from a nutrient-rich medium (food extract or BHI) to a minimum essential medium. CONCLUSIONS: In vitro virulence-associated phenotype level of the studied strains grown in BHI or cold-smoked salmon was the same as the control virulent strain EGD. In contrast, the nutrients present in rillettes may therefore substantially reduce the number of plaques but not the growth of L. monocytogenes. The utilization of minimum essential medium as diluent attenuates changes the effect of the food extract on virulence-associated phenotype in vitro. SIGNIFICANCE AND IMPACT OF THE STUDY: In the experimental design of this study, we showed that the nature of the food could affect the in vitro virulence-associated phenotype level of L. monocytogenes. 相似文献
66.
The North Sea, one of the most productive of the earth's seas and oceans, is also surrounded by some of earth's most densely
populated and heavily industrialized regions. A growing number of signals are being received which indicate that this valuable
ecosystem is increasingly under stress. This has generated a corresponding increase in concern over the steps to be taken
to protect the North Sea. While there are divergent views on what constitutes an ‘ideal’ North Sea, there is a general recognition
that any decisions that are made should be based on a good understanding of this ecosystem. The intention of this paper is
to give an overview of what is presently known, and to identify areas where more studies are needed. A brief summary of the
hydrography and the biota of the North Sea is given. Biotic and abiotic structure justify partitioning the North Sea into
three ecologically different regions: southern, central, and northern. For the most part, neither the top predators,e.g. marine birds and mammals, nor the macroalgae and sea grasses are included in this overview. 相似文献
67.
68.
秦岭大熊猫主食竹的分类学研究(I) 总被引:9,自引:0,他引:9
研究为秦岭大熊猫主食竹系列报道之一,主要报道产于陕西长青国家级自然保护区大熊猫主食竹。该保护区内共有野生禾本科竹亚科植物4属、5种,栽培逸为野生的1属、1种,其中巴山木竹(Bashania fargesii)和秦岭箭竹(Fargesia qinlingensis)为该区域大熊猫最主要的采食野生竹种,龙头竹(Fargesia dracocephala)次之,由栽培逸为野生的金竹(Phyllostachys sulpurea)为大熊猫喜爱的竹种。 相似文献
69.
Vasudeva Rao Avupati Rajendra Prasad Yejella Venkateswara Rao Parala Kishore Naidu Killari Venkata Madhava Reddy Papasani Prasad Cheepurupalli Venkateswara Rao Gavalapu Bhavani Boddeda 《Bioorganic & medicinal chemistry letters》2013,23(21):5968-5970
A series of some novel 1,3,5-triazine–Schiff base conjugates (1–32) have been synthesized and evaluated for their in vitro antimycobacterial activity against Mycobacterium tuberculosis H37Rv using Alamar Blue assay and the activity expressed as the minimum inhibitory concentration (MIC) in μg/mL. Compounds 4 (4-Methoxy-6-methyl-N-(3,4,5-trimethoxybenzylidene)-1,3,5-triazin-2-amine), 11 (4-Methoxy-6-methyl-N-(2-hydroxy-3-bromo-5-chloro-benzylidene)-1,3,5-triazin-2-amine) and 24 (4-Methoxy-6-methyl-N-(1-(2,5-dihydroxyphenyl)ethylidene)-1,3,5-triazin-2-amine) exhibited a significant activity at 3.125, 6.25 and 6.25 μg/mL, respectively, when compared with the antitubercular drugs such as ethambutol (3.125 μg/mL), pyrazinamide (6.25 μg/mL) and streptomycin (6.25 μg/mL) and it could be a potential starting point to develop new lead compounds in the fight against Mycobacterium tuberculosis H37Rv. 相似文献
70.
《Free radical research》2013,47(4):285-286
The purpose of this review is to bring together the different approaches for studying the oxidation of low density lipoproteins and try to identify some critical factors which will permit greater comparability between laboratories. These issues are discussed both in terms of the variety of exogenous mediators of oxidation applied (transition metal ions, haem proteins, azo initiators, peroxynitrite, cells etc.) and their raisons d'etre, as well as the methodologies (formation of conjugated dienes, hydroperoxides, decomposition products of lipid peroxidation, altered surface charge, macrophage uptake) applicable to the different stages of the oxidation and the factors underlying their accurate execution and interpretation. 相似文献