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71.
九种二变种山茶属植物的核型报道 总被引:5,自引:1,他引:4
本文报道了9种2变山茶属植物的核型.结果如下:Cameliahenryana:2n=2x=30=21m+8sm+1st;C.furfuracea:2n=2x=30=20m+10sm;C.wardi:2n=2x=30=18m+11m+1st;C.anlungensis:2n=2x=30=19m+9sm+2st;C.anlungensisvar.acutiperulata:2n=2x=30=19m+9sm+2st;C.pyxidiacea:2n=2x=30=20m+8sm+2st;C.pyxidiaceavar.rubituberculata:2n=2x=30=21m+8sm+1st;C.brevistyla:2n=2x=30=18m+10sm+2st;C.leptophyla:2n=2x=30=24m(1sat)+4sm(1sat)+2st;C.yunnanensis:2n=2x=30=18m+10sm+2st;C.pitardi:2n=2x=30=18m+12sm.其中,前7种2变种的核型为首次报道,比较前人的有关研究可以看出上述核型在种间较相似,以组为单位进行比较比种间比较具有更大的意义。 相似文献
72.
In this work, spray-dried lipid systems based on soy phosphatidylcholine, cholesterol and lauroyl polyoxylglycerides for entrapping Green tea polyphenols were produced. The aim was to study the effects of the encapsulating composition and spray drying conditions on the system performance and physicochemical product properties. The spray dryer powder production yield falls around 50.7?±?2.8%, which is typical for lab scale spray dryers. Wrinkled and rounded particles, with low surface porosities were generated, independent of the drying carriers (trehalose or lactose) used. The product showed high encapsulation efficiency of Green tea polyphenols, which was promptly redispersible in water. It presented low density, and good compressive and flow properties. The results herein reported confirm the feasibility of the entrapment of Green tea polyphenols in lipid-based compositions by spray drying in presence of the drying carriers evaluated. The spray-dried microparticles show high potential to be used as additive in food, nutraceutical and pharmaceutical products. 相似文献
73.
该研究采用氨基酸自动分析仪、分光光度法等对金花茶花蕾、开放花和初谢花的营养成分进行了比较分析。结果表明:金花茶茶花中主要营养成分是碳水化合物,含有丰富的水溶性糖和粗纤维。茶花中的脂肪、粗纤维和水溶性糖含量随花蕾至开放的形成过程呈增加趋势,谢花后其含量呈下降趋势。开放花中总黄酮、皂甙、儿茶素、VE含量比花蕾和谢花中含量高。金花茶花蕾、开放花和初谢花三个阶段中氨基酸总量分别是7.44、5.14、5.00 g·100 g~(-1)。金花茶茶花含有丰富的氨基酸,花蕾内氨基酸含量尤为丰富。综合表明,金花茶茶花具较高的开发利用价值。该研究结果为了解金花茶花朵不同采收期的营养组成以及金花茶花朵的开发及采收利用提供了科学依据。 相似文献
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油茶脱水素样蛋白的基因克隆与序列分析及其生理功能预测 总被引:5,自引:0,他引:5
以油茶EST文库为基础,采用5-′RACE技术,分离克隆了一个脱水素样基因的全长cDNA序列(GenBank接受号EU856537),同源分析表明其编码的208 aa的小分子蛋白(id号ACF72673)属于SK2型脱水素,命名为CoDHN2.CoDHN2的肽链内2个类K-片段间富含苏氨酸,有别于脱水素一般性结构特点,并且同油茶种子中另一类脱水素一样也具有一个十分保守的基序(EDDGQAGRRKK),这可能有利于脱水素的磷酸化和亚细胞定位;采用多种方法预测其二级结构,表明CoDHN2为内在性无规则蛋白,但其中远离C端的一个类K-片段可形成两亲性α-螺旋.CoDHN2含有较多的组氨酸残基以及良好的可溶性,推测它可结合金属离子从而减少活性氧的来源并清除活性氧,并在生理脱水时充当缓冲液.结合其它物种脱水素的研究进展,认为CoDHN2极有可能在油茶油脂合成高峰期同正在发育的脂体结合而保护脂体免受活性氧危害,这为油茶种子细胞的脂体发育研究提出了一个新的方向. 相似文献
76.
Caffeine and related purine alkaloids: biosynthesis, catabolism, function and genetic engineering 总被引:4,自引:0,他引:4
Details of the recently elucidated biosynthetic pathways of caffeine and related purine alkaloids are reviewed. The main caffeine biosynthetic pathway is a sequence consisting of xanthosine-->7-methylxanthosine-->7-methylxanthine-->theobromine-->caffeine. Genes encoding N-methyltransferases involved in three of these four reactions have been isolated and the molecular structure of N-methyltransferases investigated. Pathways for the catabolism of caffeine have also been studied, although there are currently no reports of enzymatic and genetic studies having been successfully carried out. Metabolism of purine alkaloids in species including Camellia, Coffea, Theobroma and Ilex plants is summarised, and evidence for the involvement of caffeine in chemical defense and allelopathy is discussed. Finally, information is presented on metabolic engineering that has produced coffee seedlings with reduced caffeine content, and transgenic caffeine-producing tobacco plants with enhanced disease resistance. 相似文献
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T. N. Nikolaeva N. V. Zagoskina M. N. Zaprometov 《Russian Journal of Plant Physiology》2009,56(1):45-49
The effect of hormone-like compounds at different concentrations: 2,4-D (2 × 10?6; 2 × 10?5; and 2 × 10?4M) and 1-NAA (2 × 10?7; 2 × 10?6; 2 × 10?5; 4 × 10?5, and 6 × 10?5 M) on the growth and production of phenolic compounds, including flavans and lignin, was investigated in callus culture of tea plant (Camellia sinensis L., a highly productive strain IFR ChS-2). The growth of the culture was vigorous, and production of phenolic compounds therein was efficient in the medium containing 2 × 10?5 M 2,4-D. Substitution of 1-NAA for 2,4-D in all the cases decelerated the growth of the culture. These changes were more pronounced when 2 × 10?7 and 2 × 10?6 M 1-NAA was used; in this case, biomass accumulation decreased by 1.5–2.0 times as compared with control material growing on the medium with 2 × 10?5 M 2,4-D. In the presence of 1-NAA, the content of total soluble phenolic compounds and flavans in the calli rose by 30% on the average as compared with control material. Accumulation of lignin remained essentially the same. Therefore, the replacement of 2,4-D with 1-NAA in the nutrient medium used for the growing of highly productive strain of tea plant callus did not induce considerable changes in its ability to produce phenolic compounds. 相似文献