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81.
A rapid procedure for the construction of PCR cDNA libraries from small amounts of plant tissue 总被引:6,自引:0,他引:6
Ian Jepson John Bray Gareth Jenkins Wolfgang Schuch Keith Edwards 《Plant Molecular Biology Reporter》1991,9(2):131-138
We describe a general method for the preparation of λZAP II cDNA libraries from very small amounts (<50 mg) of plant tissue.
We have achieved this by combining an efficient method for RNA extraction with a modified PCR protocol for the synthesis and
amplification of cDNA. Using this protocol we have found it possible to generate cDNA libraries containing more than 106 clones from as little as 1 μg of total RNA. 相似文献
82.
83.
By comparison with deciduous oaks, the lower yearly production of new leaves in sclerophyllous oaks is hypothesized to have several consequences on animal communities. In particular, the production of arthropod communities that feed upon the leaves should be lower in sclerophyllous than in deciduous oaks, this causing changes in breeding patterns and the demographic balance in insectivorous birds. Studies in both deciduous and sclerophyllous habitats in southern France have shown that: 1) the spring development of new leaves occurs later and more slowly in sclerophyllous than in deciduous oaks, 2) the biomass of caterpillars is much lower in sclerophyllous oak forests, and 3) there is a large variation in life history traits of the Blue Tit depending in which type of habitat they breed. Laying date occurs later and clutch size is lower in sclerophyllous habitats than in deciduous habitats. The evolution of life history traits is discussed according to whether poor sclerophyllous habitats are isolated (e.g. on Corsica) or are parts of landscapes including both habitat types. 相似文献
84.
Observations on morphological characters of milk and permanent teeth, based on 648 pairs of dental casts of 356 male and 292 female Jat children of Haryana (India) are reported. Deciduous teeth show high frequencies of bilateral winging of maxillary central incisor, Carabelli's cusp of maxillary second molar, and deflecting wrinkle of mandibular second molar. Reduction of maxillary molar cusps is more marked in males than in females. Y pattern is very common in deciduous molars. Permanent teeth have high frequencies of grooved cingulum of incisors, cingular nodule of lateral incisors and canines, and distal accessary ridge of canines. Low frequencies of Carabelli's cusp and winging are also common. The tendency towards faintly developed shovelling in milk incisors occurs more often than in the permanent teeth. 相似文献
85.
J. Weller M. Soller 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1981,59(2):73-77
Summary The production of a multi-marker strain given a series of strains each carrying a single marker will require a considerable investment in time and resources when based on a series of formal crosses. Such strains can be produced rapidly and with only minimal resources by recurrent cycles of random mating and selection, with selection based on the number of desired alleles carried. 相似文献
86.
Out of the five late Roman-early Byzantine cemeteries at Sayala, the three large burial complexes CI-III were found to represent the same population not only on the basis of 76·6% of the metric features already studied, but also of 72·9% of the 48 non-metric features examined in the present paper. The remaining features, which show different frequencies, were most probably related to some social and/or genetic isolation of the three subgroups (“lineages”) of the same basic population.The left-right asymmetry of the frequencies was practically irrelevant; sex differences were however present in one-third of the features observed.Some of the traits showed unusually high frequencies, revealing a high rate of inbreeding within a homogeneous population.To evaluate the relationships among the CI-III people and the neighbouring populations, 22 discrete traits were compared with the same traits studied in 11 Ancient Egyptian samples by Berry, Berry & Ucko (1967) and in 6 contemporary African Negro samples by Rightmire (1972). It was shown that the Sayala CI-III population does not resemble either of these groups. The fourth contemporary Sayala sample from A cemetery was also found to be not related with the CI-III sample on the basis of the same traits. The fifth contemporary Sayala sample from N cemetery was too small to be tested. Moreover, the comparison between the CI-III population and the C-group population of Sayala also revealed the lack of genetic continuity. The isolated position found in the CI-III population of Sayala agrees with the hypothesis that it would represent the Blemmyes, people originating from the Eastern Desert. 相似文献
87.
88.
Sixteen year-classes of longfin smelt, Spirinchus thaleichthys , show a consistent pattern of alternating year-class strength between even (strong) and odd (weak) years. A recent (1989) odd year-class grew as well as, if not better than, the late 1960s odd year-classes. However, fish in the even year-classes (1988 and 1990) were significantly smaller in size then the 1966 and 1968 cohorts. Associated with this reduction in growth of the even year-class fish is a decline in fecundity. Fecundity of the odd year-classes of smelt was also smaller than in the early 1960s. Mean egg diameter and gonadosomatic index were not statistically different between 1988 and 1989 year-classes but the relative fecundity was higher for the 1988 year-class than for 1989, suggesting a higher reproductive investment in the even year-class. Reduction in growth and associated reproductive traits is considered to result from a reduction in the abundance of mysids, a preferred prey species. 相似文献
89.
Victor Sebastian Scharnhorst Konrad Fiedler Thomas Frank Dietmar Moser Dominik Rabl Manuela Brandl Raja Imran Hussain Ronnie Walcher Bea Maas 《Ecology and evolution》2021,11(12):8319
- Ongoing intensification and fragmentation of European agricultural landscapes dramatically reduce biodiversity and associated functions. Enhancing perennial noncrop areas holds great potential to support ecosystem services such as ant‐mediated pest control.
- To study the potential of newly established grassland strips to enhance ant diversity and associated functions, we used hand collection data and predation experiments to investigate differences in (a) ant community composition and (b) biocontrol‐related functional traits, and (c) natural pest control across habitats in cereal fields, old grasslands, and new grassland transects of three years of age.
- Ant species diversity was similar between new and old grasslands, but significantly higher in new grasslands than in surrounding cereal fields. Contrary, ant community composition of new grasslands was more similar to cereal fields and distinct from the species pool of old grasslands. The functional trait space covered by the ant communities showed the same distribution between old and new grasslands. Pest control did not differ significantly between habitat types and therefore could not be linked to the prevalence of functional ant traits related to biocontrol services in new grasslands.
- Our findings not only show trends of convergence between old and new grasslands, but also indicate that enhancing ant diversity through new grasslands takes longer than three years to provide comparable biodiversity and functionality.
- Synthesis and applications: Newly established grasslands can increase ant species richness and abundance and provide a consistent amount of biocontrol services in agroecosystems. However, three years after their establishment, new grasslands were still dominated by common agrobiont ant species and lacked habitat specialists present in old grasslands, which require a constant supply of food resources and long colony establishment times. New grasslands represent a promising measure for enhancing agricultural landscapes but must be preserved in the longer term to promote biodiversity and resilience of associated ecosystem services.
90.
SPATIAL FLOWER PARAMETERS AND INSECT SPATIAL VISION 总被引:6,自引:0,他引:6
A. DAFNI M. LEHRER P. G. KEYAN 《Biological reviews of the Cambridge Philosophical Society》1997,72(2):239-282
The present article reviews recent and older literature on the spatial parameters that flowers display, as well as on the capacities of anthophilous insects to perceive and use these parameters for optimizing their foraging success. Although co-evolution of plants and pollinators has frequently been discussed with respect to floral colours and insect colour vision, it has rarely been assessed with respect to insect spatial vision and spatial floral cues, such as shape, pattern, size, contrast, symmetry, spatial frequency, contour density and orientation of contours. This review is an attempt to fill this gap. From experimental findings and observations on both flowers and insects, we arrive at the conclusion that all of the spatial and spatio-temporal parameters that flowers offer are relevant to the foraging task and are tuned to the insect's visual capacities and visually guided behaviour. We try, in addition, to indicate that temporal cues are closely related to spatial cues, and must therefore be included when flower–pollinator interactions are examined. We include results that show that colour vision and spatial vision have diverged over the course of evolution, particularly regarding the processing of spatio-temporal information, but that colour vision plays a role in the processing of spatial cues that are independent of temporal parameters. By presenting this review we hope to contribute to closer collaboration among scientists working in the vast fields of botany, ecology, evolution, ethology and sensory physiology. 相似文献