全文获取类型
收费全文 | 5769篇 |
免费 | 290篇 |
国内免费 | 233篇 |
出版年
2023年 | 56篇 |
2022年 | 73篇 |
2021年 | 144篇 |
2020年 | 145篇 |
2019年 | 157篇 |
2018年 | 149篇 |
2017年 | 137篇 |
2016年 | 126篇 |
2015年 | 141篇 |
2014年 | 188篇 |
2013年 | 356篇 |
2012年 | 154篇 |
2011年 | 207篇 |
2010年 | 166篇 |
2009年 | 263篇 |
2008年 | 293篇 |
2007年 | 263篇 |
2006年 | 258篇 |
2005年 | 229篇 |
2004年 | 226篇 |
2003年 | 210篇 |
2002年 | 173篇 |
2001年 | 127篇 |
2000年 | 141篇 |
1999年 | 123篇 |
1998年 | 135篇 |
1997年 | 104篇 |
1996年 | 123篇 |
1995年 | 116篇 |
1994年 | 114篇 |
1993年 | 113篇 |
1992年 | 96篇 |
1991年 | 82篇 |
1990年 | 96篇 |
1989年 | 62篇 |
1988年 | 81篇 |
1987年 | 61篇 |
1986年 | 60篇 |
1985年 | 79篇 |
1984年 | 86篇 |
1983年 | 39篇 |
1982年 | 58篇 |
1981年 | 65篇 |
1980年 | 49篇 |
1979年 | 45篇 |
1978年 | 32篇 |
1977年 | 18篇 |
1976年 | 19篇 |
1975年 | 10篇 |
1973年 | 11篇 |
排序方式: 共有6292条查询结果,搜索用时 250 毫秒
981.
Óscar J. Prado Sudeep C. Popat Gexin Chen Sharon L. Walker Javier Lafuente David Gabriel Marc A. Deshusses 《Biotechnology and bioengineering》2009,103(6):1060-1067
Many bioprocesses depend on the effective formation of a biofilm on a solid support. In the present study, three different surface treatments (sandblasting, pure‐O2 plasma, and He–O2 plasma treatments) were conducted on polypropylene (PP) Pall rings used as a support in biotrickling filters for air pollution control. The intent was to modify the ring surface and/or electrochemical properties in order to possibly improve cell adhesion, wetting properties, and possibly reduce the start‐up time and increase the performance of the biotrickling filters. The surface treatments were found to generally increase the hydrophilicity and the zeta potential of the surfaces. However, the startup and performance of lab‐scale biotrickling filters packed with treated Pall rings were not significantly different than the control with untreated rings. Cell and colloid deposition experiments conducted in flow cells showed that the treated surfaces and the hydrodynamic conditions were not favorable for cell deposition indicating that there could be significant opportunities for improving packings used in environmental bioprocess applications. Biotechnol. Bioeng. 2009;103: 1060–1067. © 2009 Wiley Periodicals, Inc. 相似文献
982.
983.
Temporal and spatial windows delimit activation of the outer ring of wingless in the Drosophila wing
Extracellular signalling molecules play many roles in the development of higher organisms. They are used reiteratively in different tissues and stages, but the response of the receiving cells is controlled in a context dependent manner. The pattern of expression of the signalling molecule Wingless/WNT in Drosophila is extraordinarily complex. We have studied the mechanism that controls its expression and function in the outer ring of the Drosophila wing hinge. Our findings indicate that wingless expression is controlled by a dual mechanism: its initial activation requires the product of zinc finger homeodomain 2 and is subsequently repressed by the product of the gene complex elbow/no ocelli. This tight regulation restricts the activation of wingless temporally and spatially. Later in development, wingless expression is maintained by an autoregulatory loop that involves the product of homothorax. We have analyzed the phenotype of a wingless allelic combination that specifically removes the outer ring, and our results show that Wingless is required to promote local proliferation of the wing base cells. Thus, cell proliferation in the proximal-distal axis is controlled by the sequential activation of wingless in the inner ring and the outer ring at different stages of development. 相似文献
984.
Aki Takimoto 《Developmental biology》2009,332(2):196-325
During endochondral bone formation, vascular invasion initiates the replacement of avascular cartilage by bone. We demonstrate herein that the cartilage-specific overexpression of VEGF-A164 in mice results in the hypervascularization of soft connective tissues away from cartilage. Unexpectedly, perichondrial tissue remained avascular in addition to cartilage. Hypervascularization of tissues similarly occurred when various VEGF-A isoforms were overexpressed in the chick forelimb, but also in this case perichondrial tissue and cartilage were completely devoid of vasculature. However, following bony collar formation, anti-angiogenic properties in perichondrial tissue were lost and perichondrial angiogenesis was accelerated by VEGF-A146, VEGF-A166, or VEGF-A190. Once the perichondrium was vascularized, osteoclast precursors were recruited from the circulation and the induction of MMP9 and MMP13 can be observed in parallel with the activation of TGF-β signaling. Neither perichondrial angiogenesis nor the subsequent cartilage vascularization was found to be accelerated by the non-heparin-binding VEGF-A122 or by the VEGF-A166ΔE162-R166 mutant lacking a neuropilin-binding motif. Hence, perichondrial angiogenesis is a prerequisite for subsequent cartilage vascularization and is differentially regulated by VEGF-A isoforms. 相似文献
985.
Neural recognition molecule NB-2/contactin 5 is expressed transiently during the first postnatal week in glutamatergic neurons of the central auditory system. Here, we investigated the effect of NB-2 deficiency on the auditory brainstem in mouse. While almost all principal neurons are wrapped with the calyces of Held in the medial nucleus of the trapezoid body (MNTB) in wild type, 8% of principal neurons in NB-2 knockout (KO) mice lack the calyces of Held at postnatal day (P) 6. At P10 and P15, apoptotic principal neurons were detected in NB-2 KO mice, but not in wild type. Apoptotic cells were also increased in the ventral cochlear nucleus (VCN) of NB-2 KO mice, which contains bushy neurons projecting to the MNTB and the lateral superior olive (LSO). At the age of 1 month, the number of principal neurons in the MNTB and of glutamatergic synapses in the LSO was reduced in NB-2 KO mice. Finally, interpeak latencies for auditory brainstem response waves II-III and III-IV were significantly increased in NB-2 KO mice. Together, these findings suggest that NB-2 deficiency causes a deficit in synapse formation and then induces apoptosis in MNTB and VCN neurons, affecting auditory brainstem function. 相似文献
986.
987.
Woosung Cho 《Experimental cell research》2009,315(7):1260-2266
Alexander disease is a fatal leukoencephalopathy caused by dominantly-acting coding mutations in GFAP. Previous work has also implicated elevations in absolute levels of GFAP as central to the pathogenesis of the disease. However, identification of the critical astrocyte functions that are compromised by mis-expression of GFAP has not yet been possible. To provide new tools for investigating the nature of astrocyte dysfunction in Alexander disease, we have established primary astrocyte cultures from two mouse models of Alexander disease, a transgenic that over-expresses wild type human GFAP, and a knock-in at the endogenous mouse locus that mimics a common Alexander disease mutation. We find that mutant GFAP, as well as excess wild type GFAP, promotes formation of cytoplasmic inclusions, disrupts the cytoskeleton, decreases cell proliferation, increases cell death, reduces proteasomal function, and compromises astrocyte resistance to stress. 相似文献
988.
Degenerative muscle fiber accelerates adipogenesis of intramuscular cells via RhoA signaling pathway
Tohru Hosoyama Naomi IshiguroKeitaro Yamanouchi Masugi Nishihara 《Differentiation; research in biological diversity》2009
In some pathological conditions such as Duchenne muscular dystrophy, it has been known that a fatty infiltration in skeletal muscle is often observed and that is also one of primary factors to induce marked decline of muscular strength. However, the mechanism of fatty infiltration, cellular origin of accumulated adipocytes and its significance are not fully understood. The fact that persistent degenerative muscle fibers are present on dystrophic muscle leads us to hypothesize that muscle fiber condition affects fatty infiltration in skeletal muscle. We employed a single fiber culture system to determine whether fiber condition affects an appearance of adipocytes on the fibers. Artificially hyper-contracted muscle fibers (HCF), generated from isolated intact fibers (IF) of rat extensor digitrum longus muscle, were maintained as non-adherent cultures for 5–7 days. Interestingly, there appeared to be considerable numbers of mature adipocytes on HCF, whereas no adipocytes were seen on IF, indicating that cells on HCF spontaneously differentiated into mature adipocytes. Activation of RhoA signaling by the addition of thrombin decreased the number of adipocytes on HCF in a dose-dependent manner, whereas the number of MyoD-positive myoblasts increased. In contrast, Y-27632, a specific inhibitor of Rho kinases (ROCK), induced adipogenic differentiation of cells derived from IF. In addition, administration of Y-27632 into mouse regenerating muscle resulted in fat accumulation in the muscle. Taken together, the present studies clearly demonstrated that muscle fiber condition affects fat accumulation in skeletal muscle and that is possibly mediated by the RhoA signaling pathway. 相似文献
989.
990.