The site distribution of adult strongyle parasites in the large intestines of horses in tropical Australia

In a study of gastro-intestinal parasites occurring in 57 horses in north Queensland, the locations where adult strongyle parasites were found in the large intestines were recorded in order to determine the preferred sites of these parasites. Differences were noted in the intensity of infection between the three sections of the large intestines with the ventral colon being the most heavily infected and having the highest number of species, followed by the dorsal colon and the caecum. The distribution of the various strongyle species between these sections of the intestines was not random. Many species showed preferences for particular sections. Changes were noted in site preferences of some species. These changes were related to season but not the age, sex and breed of the animal, nor the environment in which the animals were kept. These seasonal changes are discussed in relation to the epidemiology of equine strongyle infections in the area. There seems to be no differences in the basic site preference behaviour between strongyle species occurring in horses in the tropics and those from the temperate regions.     

Incidence of inherited enzyme activity variants in feral mouse populations

We have screened 173 wild caught mice (Mus musculus) from multiple sites in Europe for variation in the activity of 14 enzymes and found 8 different mutants with low enzyme activity; an incidence of 3.69/1000. This compares with the incidence of 3.26/1000 found for low enzyme activity variants in man (Mohrenweiser, 1981).This work was supported by an MRC project grant to G.B.     

Feral sheep on Socorro Island: facilitators of alien plant colonization and ecosystem decay

The paper examines the role of feral sheep ( Ovis aries ) in facilitating the naturalization of alien plants and degrading a formerly robust and stable ecosystem of Socorro, an isolated oceanic island in the Mexican Pacific Ocean. Approximately half of the island is still sheep-free. The other half has been widely overgrazed and transformed into savannah and prairie-like open habitats that exhibit sheet and gully erosion and are covered by a mix of native and alien invasive vegetation today. Vegetation transects in this moderately sheep-impacted sector show that a significant number of native and endemic herb and shrub species exhibit sympatric distribution patterns with introduced plants. Only one alien plant species has been recorded from any undisturbed and sheep-free island sector so far.
Socorro Island provides support for the hypothesis that disturbance of a pristine ecosystem is generally required for the colonization and naturalization of alien plants. Sheep are also indirectly responsible for the self-invasion of mainland bird species into novel island habitats and for the decline and range contraction of several endemic bird species.     

Genetic analysis of the Hispano-Breton heavy horse

Hispano‐Breton (HB) is a horse breed with a recent mixed ancestry. It was developed in the 1930s by crossing local mares with Breton draught horses imported from France. Nowadays it is considered to be in a vulnerable situation due to census decline. To genetically characterize the breed and to set up the basis for a conservation programme, we have employed two types of molecular markers: a 347‐bp D‐loop mitochondrial DNA (mtDNA) fragment and 13 microsatellite loci. A representative sample of 53 HB individuals was analysed together with a sample of 40 Pura Raza Española horses for comparison. Both types of markers revealed a high level of genetic diversity in the HB breed, emphasizing the importance of its conservation. The construction of a phylogenetic network with mtDNA sequences including various Iberian breeds and European heavy horses provided an overall picture of the ubiquitous appearance of HB matrilines with respect to other breeds and revealed the singularity of certain HB maternal lineages. Despite the high allelic richness found in HB horses, microsatellite analysis evidenced a certain degree of inbreeding as a consequence of the type of management generally used for local breeds.     

Reproductive Parameters of Female<Emphasis Type="Italic">Pan paniscus</Emphasis> and <Emphasis Type="Italic">P. troglodytes</Emphasis>: Quality versus Quantity

We investigated intra- and interspecific differences in life history and reproductive parameters in bonobos (Pan paniscus) and chimpanzees (Pan troglodytes). We compare the parameters of wild and captive females in order to shed light on the influence of habitat or specific differences or both on reproduction. We present new and additional information on reproductive parameters from captive bonobos and chimpanzees. Captive chimpanzees birth more live offspring and have a shorter interbirth interval, but experience higher infant mortality than captive bonobos. Although captive bonobo females tend to start reproduction at a younger age than chimpanzees, this is effectively only so for wild-born females of both species. Ultimately both species reach the same rate of production of offspring surviving to 5 yr. These results contrast with data from the wild. Wild bonobos tend to have higher reproductive success, a higher fertility rate and a shorter interbirth interval than wild chimpanzees. Reproduction is similar for wild and captive bonobos, which suggests that they are producing at their maximum under both conditions. Overall captive chimpanzees perform better than their wild conspecifics, probably because of lower feeding competition. Infant survival is the only specific difference not affected by captivity. Bonobo infants survive better, which suggests that chimpanzee infants are more at risk. We argue that the interspecific variation in reproductive parameters in captivity is related to the different influence of captivity on reproduction and different pressures of external sources of infant and juvenile mortality.     

Potential vectors of West Nile virus following an equine disease outbreak in Italy

In the late summer of 1998, an outbreak of equine encephalomyelitis due to West Nile virus (WNV) occurred in the Tuscany region of central Italy. The disease was detected in 14 race horses from nine localities in four Provinces: Firenze, Lucca, Pisa and Pistoia. The outbreak area included Fucecchio wetlands (1800 ha), the largest inland marsh in Italy, and the adjacent hilly Cerbaie woodlands with farms breeding horses. To detect potential vectors of WNV, entomological surveys of Fucecchio and Cerbaie were undertaken during 1999-2002 by collecting mosquito larvae from breeding sites and adult mosquitoes by several methods of sampling. Among 6023 mosquitoes (Diptera: Culicidae) collected, 11 species were identified: Aedes albopictus (Skuse), Ae. vexans (Meigen), Anopheles atroparvus Van Thiel, An. maculipennis Meigen s.s., An. plumbeus Stephens, Culex impudicus Ficalbi, Cx. pipiens L., Culiseta longiareolata Macquart), Ochlerotatus caspius (Pallas), Oc. detritus (Haliday) and Oc. geniculatus (Olivier). In Fucecchio marshes, Cx. impudicus predominated with seasonal peak densities in spring and autumn: its greatest abundance during early spring coincides with arrival of migratory birds from Africa. In Cerbaie hills, Cx. pipiens predominated with peak population density in late summer. No viruses were isolated from 665 mosquitoes processed. These findings, plus other data on Italian mosquito bionomics, suggest a possible mode of WNV transmission involving the most abundant Culex in the Fucecchio-Cerbaie areas. Culex impudicus, being partly ornithophilic, might transmit WNV from migratory to non-migratory birds during springtime; Cx. pipiens, having a broader host range, would be more likely to transmit WNV from birds to horses and, perhaps, to humans by late summer.     

Vector competence of Culicoides species and the seroprevalence of homologous neutralizing antibody in horses for six serotypes of equine encephalosis virus (EEV) in South Africa

Abstract. Field‐collected Culicoides species (Diptera: Ceratopogonidae) were fed on horse blood–virus mixtures containing one of the six serotypes of equine encephalosis virus (EEV1 to EEV6). The virus mean titres in the bloodmeals varied between 6.1 and 7.0 log₁₀TCID₅₀/mL. Of 19 Culicoides species assayed after 10 days extrinsic incubation at 23.5°C, five yielded the challenge virus, namely Culicoides (Avaritia) imicola Kieffer (EEV1–6), C. (A.) bolitinos Meiswinkel (EEV1, 2, 4, 6), C. (Meijerehelea) leucostictus Kiefer (EEV1, 2), C. (Culicoides) magnus Colaço (EEV1) and C. (Hoffmania) zuluensis de Meillon (EEV2). Virus recovery rates ranged from 0.5 to 13%. The mean levels of viral replication differed between serotypes and Culicoides species and ranged from 1.0 to 2.3 log₁₀TCID₅₀/midge. Culicoides midges shown in this study to be susceptible to oral infection with EEV are widely distributed in South Africa but differ considerably in their abundance, host preference and breeding sites. Of 1456 horses tested, 1144 (77%) had antibody to EEV. Homologous virus‐neutralizing antibodies to all six serotypes were detected in individual horses from all eight geographical provinces of South Africa. The distribution, prevalence, and the rate of exposure to individual serotypes varied significantly between regions. The potential for vectoring of EEV in the field by several Culicoides species with unique ecologies and lack of cross‐protection to re‐infection with multiple serotypes highlights some of the mechanisms that are likely to play a role in the virus' natural maintenance cycle and the highly efficient level of countrywide transmission amongst South African horses.     

Dynamic spleen mass in wild and domestic American mink

The immune system is considered to be an energetically expensive component of an individual's life history. Investment in the immune system can depend on the environment that an individual finds itself in. The American mink, Neovison vison, exists in the natural environment and on fur farms. The natural environment and mink farm differ in many ways, such as wild mink being exposed to many parasites that are less prevalent and less abundant in the domestic environment because of veterinary care. We collected free‐ranging mink from commercial trappers and domestic mink from fur farmers in Ontario and Nova Scotia, Canada, and examined relative spleen mass. Wild male mink had larger spleens than domestic mink in Nova Scotia, with a similar trend in Ontario. Female mink that escaped from farms (feral) in Nova Scotia had significantly larger spleens than their domestic counterparts on the farms. Both of these results are consistent with the prediction that the natural environment contains parasites and pathogens that require enhanced investment in the immune system. In Nova Scotia, females had larger spleens than males, whether considering wild or domestic populations. Finally, wild mink showed greater condition dependence of spleen mass than domestic populations. Further investigations should include experimental approaches such as providing veterinary care to wild populations to assess the effects of parasites and pathogens on the immune system. © 2012 The Linnean Society of London, Biological Journal of the Linnean Society, 2012, 107 , 624–631.     

Do dingoes suppress the activity of feral cats in northern Australia?

Large predators can have profound impacts on community composition. Not only do they directly affect prey abundance, they also indirectly affect prey abundance through their direct effects on smaller predators. In Australia, dingoes fill the role of a large predator and, in southern Australia, have clear impacts on introduced foxes. Their effect on introduced cats, however, is less clear. Here we present data from multiple sites across northern Australia (where foxes are absent), which reveal a negative correlation between cat and dingo activity. This relationship could arise because cats avoid areas where dingoes are active, or because cats are less abundant in areas with high dingo densities, or a combination of both. At a subset of our study sites, we experimentally reduced dingo (but not cat) abundance by poison baiting. This resulted in a 55% drop in dingo activity within 4 weeks of baiting, but without a compensatory increase in cat activity. This suggests the negative correlation between cat and dingo activity is not a simple consequence of cats reactively avoiding areas with higher dingo traffic, but rather, that there are fewer cats in areas where dingoes are more active. This study is a rare demonstration of the potential for dingoes to affect the behaviour and potentially the population size of feral cats, and therefore reduce the impact of feral cats on vulnerable native prey species.