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971.
972.
Elisa Garzo Mercedes Fernández‐Pascual Cesar Morcillo Alberto Fereres M. Luisa Gómez‐Guillamón W. Fred Tjallingii 《Insect Science》2018,25(4):631-642
Resistance of the melon line TGR‐1551 to the aphid Aphis gossypii is based on preventing aphids from ingesting phloem sap. In electrical penetration graphs (EPGs), this resistance has been characterized with A. gossypii showing unusually long phloem salivation periods (waveform E1) mostly followed by pathway activities (waveform C) or if followed by phloem ingestion (waveform E2), ingestion was not sustained for more than 10 min. Stylectomy with aphids on susceptible and resistant plants was performed during EPG recording while the stylet tips were phloem inserted. This was followed by dissection of the penetrated leaf section, plant tissue fixation, resin embedding, and ultrathin sectioning for transmission electron microscopic observation in order to study the resistance mechanism in the TGR. The most obvious aspect appeared to be the coagulation of phloem proteins inside the stylet canals and the punctured sieve elements. Stylets of 5 aphids per genotype were amputated during sieve element (SE) salivation (E1) and SE ingestion (E2). Cross‐sections of stylet bundles in susceptible melon plants showed that the contents of the stylet canals were totally clear and also, no coagulated phloem proteins occurred in their punctured sieve elements. In contrast, electron‐dense coagulations were found in both locations in the resistant plants. Due to calcium binding, aphid saliva has been hypothesized to play an essential role in preventing/suppressing such coagulations that cause occlusion of sieves plate and in the food canal of the aphid's stylets. Doubts about this role of E1 salivation are discussed on the basis of our results. 相似文献
973.
Conifer‐feeding budworms emerge from overwintering sites as small larvae in early spring, several days before budburst, and mine old needles. These early‐emerging larvae suffer considerable mortality during this foraging period as they disperse in search of available, current‐year buds. Once buds flush, surviving budworms construct feeding shelters and must complete maturation before fresh host foliage senesces and lignifies later in the summer. Late‐developing larvae suffer greater mortality and survivors have lower fecundity when feeding on older foliage. Thus, there is a seasonal trade‐off in fitness associated with host synchrony: early‐emerging budworms have a greater risk of mortality during spring dispersal but gain better access to the most nutritious foliage, while, on the other hand, late‐emerging larvae incur a lower risk during the initial foraging period but must contend with rapidly diminishing resource quality at the end of the feeding period. We investigate the balance that results from these early‐season and late‐season synchrony fitness trade‐offs using the concept of the phenological window. Parameters associated with the variation in the phenological window are used to estimate generational fitness as a function of host‐plant synchrony. Because defoliation modifies these relationships, it is also included in the analysis. We show that fitness trade‐offs characterizing the phenological window result in a robust synchrony relationship between budworm and host plant over a wide geographic range in southern British Columbia, Canada. 相似文献
974.
Enhanced production of the pharmaceutically important polyphenolic compounds in Vitex agnus castus L. shoot cultures by precursor feeding strategy 下载免费PDF全文
Ewa Skrzypczak‐Pietraszek Kamil Piska Jacek Pietraszek 《Engineering in Life Science》2018,18(5):287-297
Agitated Vitex agnus castus L. shoot cultures were established to analyse the content of selected pharmaceutically important flavonoids and phenolic acids. Two variants (selected from nine ones) of MS medium were prepared: A (BAP 1 mg/L; NAA 0.5 mg/L; GA3 0.25 mg/L) and B (BAP 2 mg/L; NAA 0.5 mg/L). The biomass was harvested after 1, 2, 3,4, 5 and 6 weeks. Four‐week cultures (variant A) were selected to perform the precursor feeding experiment. The L‐phenylalanine dose of 1.6 g/L appears to be the most advantageous. Compared to the control cultures, the content of the individual compounds increased in a range from 1.4 to 17.3‐fold (e.g. p‐coumaric acid – 17.3 fold; casticin – 4.8‐fold). The biomass from in vitro cultures is richer in neochlorogenic acid (16‐fold), p‐coumaric acid (5.3‐fold), rutin (2.8‐fold), caffeic acid (1.5‐fold) and cinaroside (1.5‐fold) than the leaves of its parent greenhouse‐cultivated plants. Extracts contained 30 mg/100 g DW of casticin, but after the hydrolysis its amount increased up to 200 mg/100 g DW and twice exceeded the content in the greenhouse leaves. The results indicate that V. agnus castus agitated shoot cultures might be considered as a potential biotechnological source of some pharmaceutically important compounds, especially casticin, rutin, neochlorogenic and p‐coumaric acids. 相似文献
975.
《Animal : an international journal of animal bioscience》2018,12(8):1662-1671
The calving interval (CI) can potentially impact the economic results of dairy farms. This study highlighted the most profitable CI and innovated by describing this optimum as a function of the feeding system of the farm. On-farm data were used to represent real farm conditions. A total of 1832 accounts of farms recorded from 2007 to 2014 provided economic, technical and feeding information per herd and per year. A multiple correspondence analysis created four feeding groups: extensive, low intensive, intensive and very intensive herds. The gross margin and some of its components were corrected to account for the effect of factors external to the farm, such as the market, biological status, etc. Then the corrected gross margin (cGMc) and its components were modelled by CI parameters in each feeding system by use of GLM. The relationship between cGMc and the proportion of cows with CI<380 days in each feeding group showed that keeping most of the cows in the herd with CI near to 1 year was not profitable for most farms (for the very intensive farms there was no effect of the proportion). Moreover, a low proportion of cows (0% to 20%) with a near-to-1-year CI was not profitable for the extensive and low intensive farms. Extending the proportion of cows with CI beyond 459 days until 635 days (i.e. data limitation) caused no significant economic loss for the extensive and low intensive farms, but was not profitable for the intensive and very intensive farms. Variations of the milk and feeding components explained mainly these significant differences of gross margin. A link between the feeding system and persistency, perceptible in the milk production and CI shown by the herd, could explain the different relationships observed between the extent of CI and the economic results in the feeding groups. This herd-level study tended to show different economic optima of CI as a function of the feeding system. A cow-level study would specify these tendencies to give CI objectives to dairy breeders as a function of their farm characteristics. 相似文献
976.
977.
Candice J.A. Skelton 《Journal of applied animal welfare science : JAAWS》2018,21(3):295-303
As the number of Tasmanian devils (Sarcophilus harrisii) in captivity increases, an understanding of captive social dynamics and behavior is becoming increasingly important. In the wild, devils are solitary, although sometimes, they congregate to feed on a large carcass. However, it is common to house devils in groups as a form of social enrichment. This study investigated how behavior at feeding time of captive Tasmanian devils varied in groups of different sizes. Observations were made of individually housed devils and devils in groups of two, three, five, and six, when presented with a carcass on which to feed. Total feeding duration ranged from 6.5 to 47.4 minutes per observation period (70 minutes). There was no significant interaction between feeding duration and group size during the experiment. Feeding duration varied daily and depended on carcass size. Social housing of Tasmanian devils enabled them to display dyadic and agonistic behaviors during feeding. Observing behaviors and learning from the outcomes of these interactions can improve husbandry techniques. Creating a captive environment that encourages natural behaviors may enhance survival in the wild following translocation. 相似文献
978.
目的:探讨结节乳头体在小鼠运动和摄食中的作用及机制。方法:选择雄性ddy小鼠,180-200 g,通过单侧植入电极损毁TMN-E2区。采用荧光金逆行追踪方法检测小鼠Me5与TMN之间的神经纤维联系;采用免疫组化方法检测小鼠TMN中组氨酸脱羧酶(HDC)免疫反应阳性细胞数;采用旷场试验箱记录小鼠全天、夜间以及白天的自主活动和摄食摄水;采用PCR检测小鼠穹窿周和下丘脑外侧区的orexin m RNA的表达。结果:荧光金逆行追踪实验显示小鼠Me5可向TMN-E2发出神经纤维投射。单侧TMN损毁,两侧TMN中HDC反应阳性细胞显著减少(P0.05),且损毁侧比未损毁侧HDC免疫反应阳性细胞数减少(P0.05)。TMN损毁对小鼠24 h自主活动和摄食摄水无明显影响。单侧TMN损毁,小鼠从暗期到光期的自主活动和摄食摄水显著减少(P0.05)。单侧TMN损毁,小鼠正常昼夜活动摄食节律无显著改变。单侧TMN损毁,小鼠穹隆周和下丘脑外侧区白天的orexin m RNA表达显著减少(P0.05)。结论:Me5与TMN之间存在神经通路,该通路可能通过调节穹隆周区或下丘脑外侧区的orexin神经元的激活从而调控摄食及相关行为的昼夜节律。 相似文献
979.
Silicon amendment to rice plants contributes to reduced feeding in a phloem‐sucking insect through modulation of callose deposition 下载免费PDF全文
Silicon (Si) uptake by Poaceae plants has beneficial effects on herbivore defense. Increased plant physical barrier and altered herbivorous feeding behaviors are documented to reduce herbivorous arthropod feeding and contribute to enhanced plant defense. Here, we show that Si amendment to rice (Oryza sativa) plants contributes to reduced feeding in a phloem feeder, the brown planthopper (Nilaparvata lugens, BPH), through modulation of callose deposition. We associated the temporal dynamics of BPH feeding with callose deposition on sieve plates and further with callose synthase and hydrolase gene expression in plants amended with Si. Biological assays revealed that BPH feeding was lower in Si‐amended than in nonamended plants in the early stages post‐BPH infestation. Histological observation showed that BPH infestation triggered fast and strong callose deposition in Si‐amended plants compared with nonamended plants. Analysis using qRT‐PCR revealed that expression of the callose synthase gene OsGSL1 was up‐regulated more and that the callose hydrolase (β‐1,3‐glucanase) gene Gns5 was up‐regulated less in Si‐amended than in nonamended plants during the initial stages of BPH infestation. These dynamic expression levels of OsGSL1 and Gns5 in response to BPH infestation correspond to callose deposition patterns in Si‐amended versus nonamended plants. It is demonstrated here that BPH infestation triggers differential gene expression associated with callose synthesis and hydrolysis in Si‐amended and nonamended rice plants, which allows callose to be deposited more on sieve tubes and sieve tube occlusions to be maintained more thus contributing to reduced BPH feeding on Si‐amended plants. 相似文献
980.
Rosa van den Berg Sander Kooijman Raymond Noordam Ashna Ramkisoensing Gustavo Abreu-Vieira Lauren L. Tambyrajah Wieneke Dijk Philip Ruppert Isabel M. Mol Barbara Kramar Rosanna Caputo Laura Sardón Puig Evelien M. de Ruiter Jan Kroon Menno Hoekstra Ronald J. van der Sluis Onno C. Meijer Ko Willems van Dijk Patrick C.N. Rensen 《Cell reports》2018,22(13):3521-3533