全文获取类型
收费全文 | 256篇 |
免费 | 0篇 |
国内免费 | 32篇 |
专业分类
288篇 |
出版年
2023年 | 11篇 |
2022年 | 4篇 |
2021年 | 6篇 |
2020年 | 7篇 |
2019年 | 12篇 |
2018年 | 3篇 |
2017年 | 6篇 |
2016年 | 3篇 |
2015年 | 3篇 |
2014年 | 4篇 |
2013年 | 9篇 |
2012年 | 6篇 |
2011年 | 8篇 |
2010年 | 5篇 |
2009年 | 14篇 |
2008年 | 11篇 |
2007年 | 7篇 |
2006年 | 9篇 |
2005年 | 21篇 |
2004年 | 18篇 |
2003年 | 12篇 |
2002年 | 18篇 |
2001年 | 14篇 |
2000年 | 12篇 |
1999年 | 8篇 |
1998年 | 3篇 |
1997年 | 7篇 |
1996年 | 8篇 |
1995年 | 8篇 |
1994年 | 10篇 |
1993年 | 7篇 |
1992年 | 10篇 |
1991年 | 3篇 |
1984年 | 1篇 |
排序方式: 共有288条查询结果,搜索用时 8 毫秒
91.
A new fermentation strategy using cell recycle membrane system was developed for the efficient production of poly(3-hydroxybutyrate) (PHB) from whey by recombinant Escherichia coli strain CGSC 4401 harboring the Alcaligenes latus polyhydroxyalkanoate (PHA) biosynthesis genes. By cell recycle, fed-batch cultivation employing an external membrane module, the working volume of fermentation could be constantly maintained at 2.3 l. The final cell concentration, PHB concentration and PHB content of 194 g l–1, 168 g l–1 and 87%, respectively, were obtained in 36.5 h by the pH-stat cell recycle fed-batch culture using whey solution concentrated to contain 280 g lactose l–1 as a feeding solution, resulting in a high productivity of 4.6 g PHB l–1 h–1. 相似文献
92.
Roukas T 《Biotechnology and bioengineering》1994,43(3):189-194
The production of ethanol from carob pod extract by free and immobilized Saccharomyces cerevisiae cells in batch and fed-batch culture was investigated. Fed-batch culture proved to be a better fermentation system for the production of ethanol than batch culture. In fed-batch culture, both free and immobilized S. cerevisiae cells gave the same maximum concentration (62 g/L) of final ethanol at an initial sugar concentration of 300 g/L and F = 167 mL/h. The maximum ethanol productivity (4.4 g/L h) was obtained with both free and immobilized cells at a substrate concentration of 300 g/L and F = 334 mL/h. In repeated fed-batch culture, immobilized S. cerevisiae cells gave a higher overall ethanol concentration compared with the free cells. The immobilized S. cerevisiae cells in Ca-alginate beads retained their ability to produce ethanol for 10 days. (c) 1994 John Wiley & Sons, Inc. 相似文献
93.
Ki Moon Park Min Woo Song Jae Heung Lee 《Biotechnology and Bioprocess Engineering》2008,13(2):197-203
Batch culture kinetics of the red yeast, Xanthophyllomyces dendrorhous SKKU 0107, revealed reduction in biomass with glucose and lower intracellular carotenoid content with fructose. Figures were
different when compared to sucrose, which is a disaccharide of glucose and fructose. In contrast, specific growth rate constant
stayed between 0.094~0.098 h−1, irrespective of the carbon sources employed. Although the uptake rate of glucose was found to be 2.9-fold faster than that
of fructose, sucrose was found to be a more suitable carbon source for the production of carotenoids by the studied strain.
When sugar cane molasses was used, both the specific growth rate constant and the intracellular carotenoid content decreased
by 27 and 17%, respectively. Compared with the batch culture using 28 g/L sugar cane molasses, fed-batch culture with the
same strain resulted in a 1.45-fold higher cell yield together with a similar level of carotenoid content in X. dendrorhous SKKU 0107. 相似文献
94.
产PCA基因工程菌M18G反复补料分批培养研究 总被引:2,自引:0,他引:2
吩嗪-1-羧酸(PCA)是一种广谱、高效的微生物源农药,采用反复补料分批培养工艺可提高PCA合成速率,为实现PCA的商业化生产打下了良好的基础。本试验针对高产PCA的基因工程菌M18G,首先在摇瓶培养条件下,用正交试验法研究了培养基中各主要营养因子葡萄糖、黄豆粉、甘油、95%乙醇等对PCA产量的影响,并确定了适合PCA分泌的最佳培养基(1L)为:葡萄糖6g,黄豆粉40g,甘油6mL,95%乙醇5.9mL;然后确定了反复补料分批培养时更换新鲜培养基的最佳时间为每次培养进行48h后、体积比例为90%。在此条件下,培养进行五个周期后,PCA的合成速率可达到2.27mg/h,是优化后分批培养的1.34倍,同时延长了培养周期,有利于提高设备使用率,降低生产成本。 相似文献
95.
Kim JH Han KC Koh YH Ryu YW Seo JH 《Journal of industrial microbiology & biotechnology》2002,29(1):16-19
Xylitol, a functional sweetener, was produced from xylose by biological conversion using Candida tropicalis ATCC 13803. Based on a two-substrate fermentation using glucose for cell growth and xylose for xylitol production, fed-batch
fermentations were undertaken to increase the final xylitol concentration. The effects of xylose and xylitol on xylitol production
rate were studied to determine the optimum concentrations for fed-batch fermentation. Xylose concentration in the medium (100
g l−1) and less than 200 g l−1 total xylose plus xylitol concentration were determined as optimum for maximum xylitol production rate and xylitol yield.
Increasing the concentrations of xylose and xylitol decreased the rate and yield of xylitol production and the specific cell
growth rate, probably because of an increase in osmotic stress that would interfere with xylose transport, xylitol flux to
secretion to cell metabolism. The feeding rate of xylose solution during the fed-batch mode of operation was determined by
using the mass balance equations and kinetic parameters involved in the equations in order to increase final xylitol concentration
without affecting xylitol and productivity. The optimized fed-batch fermentation resulted in 187 g l−1 xylitol concentration, 0.75 g xylitol g xylose−1 xylitol yield and 3.9 g xylitol l−1 h−1 volumetric productivity. Journal of Industrial Microbiology & Biotechnology (2002) 29, 16–19 doi:10.1038/sj.jim.7000257
Received 15 October 2001/ Accepted in revised form 30 March 2002 相似文献
96.
97.
High demand in manufactured biologics drives the continued need for increased productivity. In this study elevated lactate metabolization resulted in improved metabolic efficiency and cellular productivity for a readily intensified high titer fed-batch process. Scheduled base or lactate feeds during the stationary growth phase led to increased titers (+9% and +8% respectively) without impacting the overall growth performance. The higher lactate consumption induced by either feed strategy substituted for glutamate catabolism and consequently reduced ammonia build-up. Direct correlation between increased titers and reduced ammonia levels was shown. Product quality attributes were impacted by both feeding strategies but could be matched with the control process by shortening the cell culture duration while maintaining titer constant. 相似文献
98.
Controlled nitrate feeding strategies for fed-batch cultures of microalgae were applied for the enhancement of lipid production and microalgal growth rates. In particular, in this study, the effect of nitrate feeding rates on lipid and biomass productivities in fed-batch cultures of Nannochloropsis gaditana were investigated using three feeding modes (i.e., pulse, continuous, and staged) and under two light variations on both lipid productivity and fatty acid compositions. Higher nitrate levels negatively affected lipid production in the study. Increasing the light intensity increased the lipid contents of the microalgae in all three fed-batch feeding modes. A maximum of 58.3% lipid- to dry weight ratio was achieved when using pulse-fed cultures at an illumination of 200 μmol photons m−2 s−1 and 10 mg/day of nitrate feeding. This condition also resulted in the maximum lipid productivity of 44.6 mg L−1 day−1. The fatty acid compositions of the lipids consisted predominantly of long-chain fatty acids (C:16 and C:18) and accounted for 70% of the overall fatty acid methyl esters. Pulse feeding mode was found to significantly enhance the biomass and lipid production. The other two feeding modes (continuous and staged) were not ideal for lipid and biomass production. This study demonstrates the applicability of pulse feeding strategies in fed-batch cultures as an appropriate cultivation strategy that can increase both lipid accumulation and biomass production. 相似文献
99.
Kahina Mellahi Florian Cambay Denis Brochu Michel Gilbert Michel Perrier Sven Ansorge Yves Durocher Olivier Henry 《Biotechnology progress》2019,35(1):e2742
Inducible mammalian expression systems are becoming increasingly available and are not only useful for the production of cytotoxic/cytostatic products, but also confer the unique ability to uncouple the growth and production phases. In this work, we have specifically investigated how the cell culture state at the time of induction influences the cumate-inducible expression of recombinant rituximab by a GS-CHO cell line. To this end, cells grown in batch and fed-batch cultures were induced at increasing cell densities (1 to 10 × 10 6 cells/mL). In batch, the cell specific productivity and the product yield were found to reduce with increasing cell density at induction. A dynamic feeding strategy using a concentrated nutrient solution applied prior and postinduction allowed to significantly increase the integral of viable cells and led to a 3-fold increase in the volumetric productivity (1.2 g/L). The highest product yields were achieved for intermediate cell densities at induction, as cultures induced during the late exponential phase (10 × 10 6 cells/mL) were associated with a shortened production phase. The final glycosylation patterns remained however similar, irrespective of the cell density at induction. The kinetics of growth and production in a 2 L bioreactor were largely comparable to shake flasks for a similar cell density at induction. The degree of galactosylation was found to decrease over time, but the final glycan distribution at harvest was consistent to that of the shake flasks cultures. Taken together, our results provide useful insights for the rational development of fed-batch cell culture processes involving inducible CHO cells. © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 35: e2742, 2019 相似文献
100.
Growth of Bacillus subtilis TN106[pAT5] and synthesis of plasmid-encoded protein (alpha-amylase) are investigated in batch, continuous, and fed-batch cultures using a defined medium containing glucose and/or starch as the carbohydrate source. The batch culture studies reveal that reduced availability of arginine hampers growth of recombinant cells (which lack an arginine synthesis gene) but promotes production of alpha-amylase and substitution of glucose by starch as the carbohydrate source leads to slower growth of recombinant cells and increased production of alpha-amylase per unit cell mass. Retention of recombinant cells over prolonged periods in continuous cultures is not possible without continuous application of antibiotic selection pressure owing to segregational plasmid instability. Fed-batch experiments with constant volumetric feed rate demonstrate that alpha-amylase production is enhanced at lower feed concentration of starch (sole carbohydrate source) and lower volumetric feed rate. Such slow addition of starch is however not conducive for growth of recombinant cells. The expression of the thermostable alpha-amylase gene carried on the recombinant plasmid pAT5 (derived from a plasmid isolated from a thermophilic bacterium) is promoted at higher temperatures, while growth of recombinant cells is depressed. In all batch and fed-batch experiments, production of alpha-amylase is observed to be inversely related to growth of recombinant cells. The efficacy of two-stage bioreactor operations, with growth of recombinant cells being promoted in the first stage and alpha-amylase production in the second stage, in attaining increased bulk alpha-amylase activity is demonstrated. (c) 1993 John Wiley & Sons, Inc. 相似文献