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241.
Plants often tolerate water deficits by lowering the osmotic potential of their cell sap. This may be achieved by accumulation of solutes which results in the maintenance of a positive turgor potential. In this study, the effect of water deficit on sugar uptake was investigated in leaf discs of Phaseolus coccinius L. (cv. Scarlet). Evidence is presented that cell turgor affects the kinetics of sugar transport at the membrane level. Uptake kinetics of sucrose, glucose and 3-O-methyl glucose by tissues equilibrated in solutions of relatively high (200–400 mOsm) osmotic concentration consisted of a sat-urable and a linear component. Low external osmotic concentration i.e., high cellular turgor inhibited the saturating component of sucrose uptake, resulting in a linear uptake profile. However, high cell turgor had no effect on glucose or 3-O-methyl glucose uptake kinetics. The effect of turgor versus osmotic component of water potential was differentiated by comparing responses to non-penetrating (manmtol) or polyethylene glycol, (3350) and penetrating (ethylene glycal) osmotica. Changes in sucrose uptake rates and kinetics were due to changes in cellular turgor and not osmotic potential. Furthermore, at low cellular turgor, a net increase in sucrose uptake occurred as a consequence of enhanced influx rates and not as a result of reduced efflux rates. The data are consistent with previous findings that sugar uptake rates are enhanced under low turgor. We present first evidence indicating that the mechanism by which higher rates of sucrose uptake are maintained underwater deficit conditions is by the activation of the saturable transport system. This mechanism supports previous suggestions that changes in cell turgor are sensed and manifested at the membrane level. 相似文献
242.
Following restoration measures (ring canalization, treatment plant with phosphorus precipitation), phosphorus loading declined step-wise from 26.21 t year–1 to 9.18 t year–1 during the period 1979–1984 while P-retention increased from 48% to 78%. Phosphorus loading was poorely correlated with precipitation. Inorganic nitrogen load, largely NO3– N, did not decline but was significantly correlated with precipitation (r = 0.95) throughout the investigation period (1978–1989).Total phosphorus loading reached acceptable levels in 1984 when compared to critical loading calculated according to Vollenweider (1976). Phosphorus input to the lake has remained at these levels in recent years.Average annual chlorophyll-a concentrations and biovolume of phytoplankton in the top 20 m layer of the lake decreased, in correspondence with the respective phosphorus concentrations, from eutrophic to mesotrophic levels. The decline was accompanied by a drastic reduction of blue-green algal populations, and especially of Oscillatoria rubescens D.C.. 相似文献
243.
Xin Wang Jin Wu Min Chen Xiangtao Xu Zhenhua Wang Bin Wang Chengzhang Wang Shilong Piao Weili Lin Guofang Miao Meifeng Deng Chunlian Qiao Jing Wang Shan Xu Lingli Liu 《Global Change Biology》2018,24(10):4983-4992
Theoretical and eddy covariance studies demonstrate that aerosol‐loading stimulates canopy photosynthesis, but field evidence for the aerosol effect on tree growth is limited. Here, we measured in situ daily stem growth rates of aspen trees under a wide range of aerosol‐loading in China. The results showed that daily stem growth rates were positively correlated with aerosol‐loading, even at exceptionally high aerosol levels. Using structural equation modeling analysis, we showed that variations in stem growth rates can be largely attributed to two environmental variables covarying with aerosol loading: diffuse fraction of radiation and vapor pressure deficit (VPD). Furthermore, we found that these two factors influence stem growth by influencing photosynthesis from different parts of canopy. Using field observations and a mechanistic photosynthesis model, we demonstrate that photosynthetic rates of both sun and shade leaves increased under high aerosol‐loading conditions but for different reasons. For sun leaves, the photosynthetic increase was primarily attributed to the concurrent lower VPD; for shade leaves, the positive aerosol effect was tightly connected with increased diffuse light. Overall, our study provides the first field evidence of increased tree growth under high aerosol loading. We highlight the importance of understanding biophysical mechanisms of aerosol‐meteorology interactions, and incorporating the different pathways of aerosol effects into earth system models to improve the prediction of large‐scale aerosol impacts, and the associated vegetation‐mediated climate feedbacks. 相似文献
244.
In Situ Assembly of 2D Conductive Vanadium Disulfide with Graphene as a High‐Sulfur‐Loading Host for Lithium–Sulfur Batteries 下载免费PDF全文
Xingyu Zhu Wen Zhao Yingze Song Qiucheng Li Feng Ding Jingyu Sun Li Zhang Zhongfan Liu 《Liver Transplantation》2018,8(20)
Lithium–sulfur (Li–S) batteries are deemed to be one of the most promising energy storage technologies because of their high energy density, low cost, and environmental benignancy. However, existing drawbacks including the shuttling of intermediate polysulfides, the insulating nature of sulfur, and the considerable volume change of sulfur cathode would otherwise result in the capacity fading and unstable cycling. To overcome these challenges, herein an in situ assembly route is presented to fabricate VS2/reduced graphene oxide nanosheets (G–VS2) as a sulfur host. Benefiting from the 2D conductive and polar VS2 interlayered within a graphene framework, the obtained G–VS2 hybrids can effectively suppress the polysulfide shuttling, facilitate the charge transport, and cushion the volume expansion throughout the synergistic effect of structural confinement and chemical anchoring. With these advantageous features, the obtained sulfur cathode (G–VS2/S) can deliver an outstanding rate capability (≈950 and 800 mAh g?1 at 1 and 2 C, respectively) and an impressive cycling stability at high rates (retaining ≈532 mAh g?1 after 300 cycles at 5 C). More significantly, it enables superior cycling performance of high‐sulfur‐loading cathodes (achieving an areal capacity of 5.1 mAh cm?2 at 0.2 C with a sulfur loading of 5 mg cm?2) even at high current densities. 相似文献
245.
Physiological responses to swimming fatigue in juvenile largemouth bronze gudgeon Coreius guichenoti 下载免费PDF全文
X. Yuan Y. P. Huang L. Cai D. Johnson Z. Y. Tu Y. H. Zhou 《Journal of fish biology》2018,92(4):1192-1197
Stepped velocity tests were conducted on juvenile largemouth bronze gudgeon Coreius guichenoti in a swim tunnel respirometer, and oxygen consumption increased with swimming speed to fatigue and then decreased during recovery. Serum levels of total protein, glucose and triglycerides initially decreased, increased at fatigue and then decreased during recovery. Levels stabilized after 120 min, corresponding to the time necessary to recover from fatigue. 相似文献
246.
A three‐phase excess post‐exercise oxygen consumption in Atlantic salmon Salmo salar and its response to exercise training 下载免费PDF全文
Y. Zhang G. Claireaux H. Takle S. M. Jørgensen A. P. Farrell 《Journal of fish biology》2018,92(5):1385-1403
The recovery of oxygen uptake to the standard metabolic rate (SMR) following exhaustive chasing exercise in Atlantic salmon Salmo salar parr occurred in three phases (rapid, plateau and slow). The initial recovery phase lasted 0·7 h and contributed 16% to the total excess post‐exercise oxygen consumption (EPOC). It was followed by a longer plateau phase that contributed 53% to the total EPOC. The slow recovery phase that completed recovery of SMR, which has not been reported previously, made a 31% contribution to the total EPOC. The plasticity of EPOC was demonstrated in exercise‐trained fish. Exercise training increased EPOC by 39% when compared with control fish (mean ± S.E., 877·7 ± 73·1 v . 629·2 ± 53·4 mg O2 kg?1, d.f. = 9, P < 0·05), with the duration of the plateau phase increasing by 38% (4·7 ± 0·58 v . 3·4 ± 0·16 h, d.f. = 9, P < 0·05) and the contribution of the slow phase to the total EPOC increasing by 80% (173·9 ± 23·9 v . 312·5 ± 50·4 mg O2 kg?1, d.f. = 9, P < 0·05). As a result, the combination of the plateau and slow phases of exercise‐trained fish increased by 47% compared with control fish (756·6 ± 71·4 v . 513·6 ± 43·1 mg O2 kg?1; d.f. = 9, P = 0·01). To substantiate the hypothesis that the plateau and slow recovery phase of EPOC was related to general metabolic recovery following exhaustive exercise, the time‐course for recovery of SMR was compared with previously published metabolite recovery profiles. The final phase of metabolic recovery was temporally associated with the final phases of gluconeogenesis, lactate oxidation and muscle intracellular pH regulation. Therefore, the plasticity of the latter phase of EPOC agreed with the known effects of exercise training in fishes. 相似文献
247.
Xiuhong Cai Kamran Haider Jianxun Lu Slaven Radic Chang Yun Son Qiang Cui M.R. Gunner 《BBA》2018,1859(10):997-1005
Cytochrome c Oxidase (CcO) reduces O2, the terminal electron acceptor, to water in the aerobic, respiratory electron transport chain. The energy released by O2 reductions is stored by removing eight protons from the high pH, N-side, of the membrane with four used for chemistry in the active site and four pumped to the low pH, P-side. The proton transfers must occur along controllable proton pathways that prevent energy dissipating movement towards the N-side. The CcO N-side has well established D- and K-channels to deliver protons to the protein interior. The P-side has a buried core of hydrogen-bonded protonatable residues designated the Proton Loading Site cluster (PLS cluster) and many protonatable residues on the P-side surface, providing no obvious unique exit. Hydrogen bond pathways were identified in Molecular Dynamics (MD) trajectories of Rb. sphaeroides CcO prepared in the PR state with the heme a3 propionate and Glu286 in different protonation states. Grand Canonical Monte Carlo sampling of water locations, polar proton positions and residue protonation states in trajectory snapshots identify a limited number of water mediated, proton paths from PLS cluster to the surface via a (P-exit) cluster of residues. Key P-exit residues include His93, Ser168, Thr100 and Asn96. The hydrogen bonds between PLS cluster and P-exit clusters are mediated by a water wire in a cavity centered near Thr100, whose hydration can be interrupted by a hydrophobic pair, Leu255B (near CuA) and Ile99. Connections between the D channel and PLS via Glu286 are controlled by a second, variably hydrated cavity.
Significance statement
Cytochrome C oxidase plays a crucial role in cellular respiration and energy generation. It reduces O2 to water and uses the released free energy to move protons across mitochondrial and bacterial cell membranes adding to the essential electrochemical gradient. Energy storage requires that protons are taken up from the high pH, N-side and released to the low pH, P-side of the membrane. We identify a potential proton exit from a buried cluster of polar residues (the proton loading site) to the P-side of CcO via paths made up of waters and conserved residues. Two water cavities connect the proton exit pathway to the surface only when hydrated. Changing the degree of hydration may control otherwise energetically favorable proton backflow from the P-side. 相似文献248.
249.
250.
Yuichi Tsuda Kaori Iwasawa Makoto Yamaguchi 《Bioscience, biotechnology, and biochemistry》2018,82(5):856-861
We investigated the respective effects of the acute supplementation of valine, leucine, and isoleucine on metabolism-related markers by administering a swimming exercise test to rats. As a behavioral analysis, we evaluated the effect of valine and that of leucine on spontaneous activity after exercise. Acute supplementation of valine before exercise significantly suppressed the depression of the liver glycogen and the blood glucose after exercise, whereas leucine decreased the blood glucose and isoleucine had no effect. Valine or leucine supplementation significantly decreased the plasma corticosterone level after exercise, while isoleucine had no effect. In the behavioral analysis, valine significantly increased the spontaneous activity after exercise, whereas leucine had no effect. These results indicate that in rats, the acute supplementation of valine, not leucine or isoleucine, is effective for maintaining liver glycogen and blood glucose and increasing spontaneous activity after exercise, which could contribute to the reduction of fatigue during exercise. 相似文献