全文获取类型
收费全文 | 1703篇 |
免费 | 267篇 |
国内免费 | 93篇 |
出版年
2024年 | 7篇 |
2023年 | 38篇 |
2022年 | 41篇 |
2021年 | 69篇 |
2020年 | 69篇 |
2019年 | 80篇 |
2018年 | 71篇 |
2017年 | 86篇 |
2016年 | 81篇 |
2015年 | 121篇 |
2014年 | 154篇 |
2013年 | 146篇 |
2012年 | 96篇 |
2011年 | 87篇 |
2010年 | 81篇 |
2009年 | 76篇 |
2008年 | 68篇 |
2007年 | 88篇 |
2006年 | 79篇 |
2005年 | 61篇 |
2004年 | 49篇 |
2003年 | 48篇 |
2002年 | 37篇 |
2001年 | 33篇 |
2000年 | 28篇 |
1999年 | 35篇 |
1998年 | 23篇 |
1997年 | 24篇 |
1996年 | 21篇 |
1995年 | 11篇 |
1994年 | 16篇 |
1993年 | 8篇 |
1992年 | 15篇 |
1991年 | 15篇 |
1990年 | 9篇 |
1989年 | 5篇 |
1987年 | 11篇 |
1986年 | 5篇 |
1985年 | 4篇 |
1984年 | 12篇 |
1983年 | 5篇 |
1982年 | 3篇 |
1981年 | 9篇 |
1980年 | 8篇 |
1979年 | 3篇 |
1978年 | 5篇 |
1977年 | 5篇 |
1975年 | 7篇 |
1974年 | 2篇 |
1971年 | 2篇 |
排序方式: 共有2063条查询结果,搜索用时 15 毫秒
61.
Kai T. Lohbeck Ulf Riebesell Sinéad Collins Thorsten B. H. Reusch 《Evolution; international journal of organic evolution》2013,67(7):1892-1900
Predicting the impacts of environmental change on marine organisms, food webs, and biogeochemical cycles presently relies almost exclusively on short‐term physiological studies, while the possibility of adaptive evolution is often ignored. Here, we assess adaptive evolution in the coccolithophore Emiliania huxleyi, a well‐established model species in biological oceanography, in response to ocean acidification. We previously demonstrated that this globally important marine phytoplankton species adapts within 500 generations to elevated CO2. After 750 and 1000 generations, no further fitness increase occurred, and we observed phenotypic convergence between replicate populations. We then exposed adapted populations to two novel environments to investigate whether or not the underlying basis for high CO2‐adaptation involves functional genetic divergence, assuming that different novel mutations become apparent via divergent pleiotropic effects. The novel environment “high light” did not reveal such genetic divergence whereas growth in a low‐salinity environment revealed strong pleiotropic effects in high CO2 adapted populations, indicating divergent genetic bases for adaptation to high CO2. This suggests that pleiotropy plays an important role in adaptation of natural E. huxleyi populations to ocean acidification. Our study highlights the potential mutual benefits for oceanography and evolutionary biology of using ecologically important marine phytoplankton for microbial evolution experiments. 相似文献
62.
Lucas Molleman Eva van den Broek Martijn Egas 《Proceedings. Biological sciences / The Royal Society》2013,280(1757)
There is ample evidence that human cooperative behaviour towards other individuals is often conditioned on information about previous interactions. This information derives both from personal experience (direct reciprocity) and from experience of others (i.e. reputation; indirect reciprocity). Direct and indirect reciprocity have been studied separately, but humans often have access to both types of information. Here, we experimentally investigate information use in a repeated helping game. When acting as donor, subjects can condition their decisions to help recipients with both types of information at a small cost to access such information. We find that information from direct interactions weighs more heavily in decisions to help, and participants tend to react less forgivingly to negative personal experience than to negative reputation. Moreover, effects of personal experience and reputation interact in decisions to help. If a recipient''s reputation is positive, the personal experience of the donor has a weak effect on the decision to help, and vice versa. Yet if the two types of information indicate conflicting signatures of helpfulness, most decisions to help follow personal experience. To understand the roles of direct and indirect reciprocity in human cooperation, they should be studied in concert, not in isolation. 相似文献
63.
Felipe Dargent Marilyn E. Scott Andrew P. Hendry Gregor F. Fussmann 《Proceedings. Biological sciences / The Royal Society》2013,280(1773)
A reduction in the strength of selection is expected to cause the evolution of reduced trait expression. Elimination of a parasite should thus cause the evolution of reduced resistance to that parasite. To test this prediction in nature, we studied the fourth- and eighth-generation descendants of guppies (Poecilia reticulata) introduced into four natural streams following experimental elimination of a common and deleterious parasite (Gyrodactylus spp.). After two generations of laboratory rearing to control for plasticity and maternal effects, we infected individual fish to assess their resistance to the parasite. Contrary to theoretical expectations, the introduced guppy populations had rapidly and repeatably evolved increased resistance to the now-absent parasite. This evolution was not owing to a resistance-tolerance trade-off, nor to differences in productivity among the sites. Instead, a leading candidate hypothesis is that the rapid life-history evolution typical in such introductions pleiotropically increases parasite resistance. Our study adds a new dimension to the growing evidence for contemporary evolution in the wild, and also points to the need for a re-consideration of simple expectations from host–parasite theory. In particular, our results highlight the need for increased consideration of multiple sources of selection and pleiotropy when studying evolution in natural contexts. 相似文献
64.
医院绩效管理中存在倾向于刚性管理并热衷于惩罚方式的现象,其结果是无意中起了引导员工为逃避惩罚而隐瞒相关信息的作用,从而使医院员工不能共同分享与讨论各种经验教训,不利于医院进行持续质量改进。而有效预防与解决这一问题的主要策略是在绩效管理中进行人性化管理,并以柔性化操作处理那些不易进行刚性考核或易在刚性考核中产生消极后果的问题。 相似文献
65.
《Bioscience, biotechnology, and biochemistry》2013,77(12):3098-3101
Microfluid filters were fabricated, which possessed 2,100 cylindrical through-bores (φ40 μm) in 200 μm-thickness polymethylmethacrylate (PMMA) sheets (φ3 mm), by deep X-ray lithography using synchrotron radiation. To evaluate the microfluid filters as a device for an immunoassay, we bound the goat anti-mouse immunogloblin G (IgG) antibody to the surface of the filters, and set the filters between reaction vessels stacked vertically in a microreactor. An enzyme-linked immunosorbent assay (ELISA) of mouse IgG using the goat anti-mouse IgG/horseradish-peroxidase (HRP) conjugate indicated that mouse IgG could be quantitatively detected in the range of 0–100 ng/ml, demonstrating the applicability of vertical microfluidic operation to the immunoassay. 相似文献
66.
目的了解妇科恶性肿瘤术后患者阴道微生态的状况,探讨妇科恶性肿瘤术后阴道微生态评价的临床意义。方法选取100例妇科恶性肿瘤术后复查的患者,取阴道分泌物进行微生态评价,以同期30例绝经体检妇女作为对照。结果妇科恶性肿瘤术后阴道优势菌以乳酸杆菌为主的,占41%;菌群密集度多在+ + ~+ + +,占52%;菌群多样性多在+ + ~+ + +,44% ;PH^4.5,占35% ;VVC阳性率为4% ;阴道毛滴虫阳性率为7% ;BV阳性率为5% ;微生态失调的发生率占69%。结论(1)妇科恶性肿瘤术后,阴道微生态失调发生率明显增高;(2)妇科恶性肿瘤术后阴道微生态的失调与肿瘤的种类、恶性程度、手术范围、卵巢去留等无关,但术后时间越长,阴道微生态失调率越高;(3)评价妇科恶性肿瘤术后阴道微生态环境的状况,对预防与治疗术后生殖道感染具有重要指导意义。 相似文献
67.
Alexander V. Semenov Jan Dirk van Elsas Debora C. M. Glandorf Menno Schilthuizen Willem F. de Boer 《Ecology and evolution》2013,3(8):2739-2750
To fulfill existing guidelines, applicants that aim to place their genetically modified (GM) insect‐resistant crop plants on the market are required to provide data from field experiments that address the potential impacts of the GM plants on nontarget organisms (NTO's). Such data may be based on varied experimental designs. The recent EFSA guidance document for environmental risk assessment (2010) does not provide clear and structured suggestions that address the statistics of field trials on effects on NTO's. This review examines existing practices in GM plant field testing such as the way of randomization, replication, and pseudoreplication. Emphasis is placed on the importance of design features used for the field trials in which effects on NTO's are assessed. The importance of statistical power and the positive and negative aspects of various statistical models are discussed. Equivalence and difference testing are compared, and the importance of checking the distribution of experimental data is stressed to decide on the selection of the proper statistical model. While for continuous data (e.g., pH and temperature) classical statistical approaches – for example, analysis of variance (ANOVA) – are appropriate, for discontinuous data (counts) only generalized linear models (GLM) are shown to be efficient. There is no golden rule as to which statistical test is the most appropriate for any experimental situation. In particular, in experiments in which block designs are used and covariates play a role GLMs should be used. Generic advice is offered that will help in both the setting up of field testing and the interpretation and data analysis of the data obtained in this testing. The combination of decision trees and a checklist for field trials, which are provided, will help in the interpretation of the statistical analyses of field trials and to assess whether such analyses were correctly applied. 相似文献
68.
Stephen Craven Nishikant Shirsat Jessica Whelan Brian Glennon 《Biotechnology progress》2013,29(1):186-196
A Monod kinetic model, logistic equation model, and statistical regression model were developed for a Chinese hamster ovary cell bioprocess operated under three different modes of operation (batch, bolus fed‐batch, and continuous fed‐batch) and grown on two different bioreactor scales (3 L bench‐top and 15 L pilot‐scale). The Monod kinetic model was developed for all modes of operation under study and predicted cell density, glucose glutamine, lactate, and ammonia concentrations well for the bioprocess. However, it was computationally demanding due to the large number of parameters necessary to produce a good model fit. The transferability of the Monod kinetic model structure and parameter set across bioreactor scales and modes of operation was investigated and a parameter sensitivity analysis performed. The experimentally determined parameters had the greatest influence on model performance. They changed with scale and mode of operation, but were easily calculated. The remaining parameters, which were fitted using a differential evolutionary algorithm, were not as crucial. Logistic equation and statistical regression models were investigated as alternatives to the Monod kinetic model. They were less computationally intensive to develop due to the absence of a large parameter set. However, modeling of the nutrient and metabolite concentrations proved to be troublesome due to the logistic equation model structure and the inability of both models to incorporate a feed. The complexity, computational load, and effort required for model development has to be balanced with the necessary level of model sophistication when choosing which model type to develop for a particular application. © 2012 American Institute of Chemical Engineers Biotechnol. Prog., 2013 相似文献
69.
《Archives of animal nutrition》2013,67(5):436-442
Abstract Histomoniasis (infectious enterohepatitis, blackhead) is caused by the protozoan parasite Histomonas meleagridis (H. meleagridis). After the ban of all prophylactic and therapeutic drugs in the European Union, histomoniasis is increasingly responsible for considerable economic problems to the poultry industry. The aim of this study was to investigate the effect of a herbal product with extracts from cinnamon, garlic, lemon, and rosemary on H. meleagridis in turkey poults in vivo. For this purpose, 60 two-week-old poults were divided into three groups. Group 1 received the herbal product in the feed six days before infection and in water three days before infection, then in feed and drinking water until the end of the experiment. Groups 2 and 3 were left untreated. At week 3 of age, Groups 1 and 2 were infected intracloacally with H. meleagridis. Three weeks after infection the surviving birds were euthanized and examined for pathological lesions. Mortality was 20% in Group 1 and 50% in Group 2. There were no deaths in Group 3. DNA of histomonads was detected in all examined caeca and livers of the dead birds, but was not detected in any examined organ of the surviving birds of all groups. There was no noticeable difference in the lesion scores of the dead birds between the groups. The surviving birds of all groups did not show lesions post mortem. Since all effective prophylactic and therapeutic drugs against histomoniasis were banned in the EU, under given conditions the investigated herbal product seems to be an effective alternative for the reduction of mortality in turkeys caused by histomoniasis. 相似文献
70.
Ryan T Jones Sara M Vetter John Montenieiri Jennifer Holmes Scott A Bernhardt Kenneth L Gage 《The ISME journal》2013,7(1):224-228
We collected Oropsylla montana from rock squirrels, Spermophilus varigatus, and infected a subset of collected fleas with Yersinia pestis, the etiological agent of plague. We used bar-tagged DNA pyrosequencing to characterize bacterial communities of wild, uninfected controls and infected fleas. Bacterial communities within Y. pestis-infected fleas were substantially more similar to one another than communities within wild or control fleas, suggesting that infection alters the bacterial community in a directed manner such that specific bacterial lineages are severely reduced in abundance or entirely eliminated from the community. Laboratory conditions also significantly altered flea-associated bacterial communities relative to wild communities, but much less so than Y. pestis infection. The abundance of Firmicutes decreased considerably in infected fleas, and Bacteroidetes were almost completely eliminated from both the control and infected fleas. Bartonella and Wolbachia were unaffected or responded positively to Y. pestis infection. 相似文献