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131.
D. G. Milchunas † J. A. Morgan‡ A. R. Mosier§† D. R. LeCain§ 《Global Change Biology》2005,11(10):1837-1855
The dynamics and demography of roots were followed for 5 years that spanned wet and drought periods in native, semiarid shortgrass steppe grassland exposed to ambient and elevated atmospheric CO2 treatments. Elevated compared with ambient CO2 concentrations resulted in greater root‐length growth (+52%), root‐length losses (+37%), and total pool sizes (+41%). The greater standing pool of roots under elevated compared with ambient CO2 was because of the greater number of roots (+35%), not because individuals were longer. Loss rates increased relatively less than growth rates because life spans were longer (+41%). The diameter of roots was larger under elevated compared with ambient CO2 only in the upper soil profile. Elevated CO2 affected root architecture through increased branching. Growth‐to‐loss ratio regressions to time of equilibrium indicate very long turnover times of 5.8, 7.0, and 5.3 years for control, ambient, and elevated CO2, respectively. Production was greater under elevated compared with ambient CO2 both below‐ and aboveground, and the above‐ to belowground ratios did not differ between treatments. However, estimates of belowground production differed among methods of calculation using minirhizotron data, as well as between minirhizotron and root‐ingrowth methods. Users of minirhizotrons may need to consider equilibration in terms of both new growth and disappearance, rather than just growth. Large temporal pulses of root initiation and termination rates of entire individuals were observed (analogous to birth–death rates), and precipitation explained more of the variance in root initiation than termination. There was a dampening of the pulsing in root initiation and termination under elevated CO2 during both wet and dry periods, which may be because of conservation of soil water reducing the suddenness of wet pulses and duration and severity of dry pulses. However, a very low degree of synchrony was observed between growth and disappearance (production and decomposition). 相似文献
132.
Toc75 is postulated to form the protein translocation channel in the chloroplastic outer envelope membrane. Proteins homologous to Toc75 are present in a wide range of organisms, with the closest homologs occurring in cyanobacteria. Therefore, an endosymbiotic origin of Toc75 has been postulated. Recently, a gene encoding a paralog to Toc75 was identified in Arabidopsis and its product was named atToc75-V. In the present study, we characterized this new Toc75 paralog, and investigated extensively the relationships among Toc75 homologs from higher plants and bacteria in order to gain insights into the evolutionary origin of the chloroplastic protein translocation channel. First, we found that the native molecular weight of atToc75-V is 80 kDa and renamed it (AtOEP80) Arabidopsis thalianaouter envelope protein of 80 kDa. Second, we found that AtOEP80 and Toc75 utilize different mechanisms for their targeting to the chloroplastic envelope. Toc75 is directed with a cleavable bipartite transit peptide partly via the general import pathway, whereas AtOEP80 contains the targeting information within its mature sequence, and its targeting is independent of the general pathway. Third, we undertook phylogenetic analyses of Toc75 homologs from various organisms, and found that Toc75 and OEP80 represent two independent gene families that are most likely derived from cyanobacterial sequences. Our results suggest that Toc75 and OEP80 diverged early in the evolution of plastids from their common ancestor with modern cyanobacteria. 相似文献
133.
Mark Deakin Steve Curwell Patrizia Lombardi 《The International Journal of Life Cycle Assessment》2001,6(6):373-383
This paper has outlined the areas of the Environment and Climate Programme (Economic and Social Aspects of Human Settlement)
the BEQUEST project addresses. It has also examined the framework for analysis the project sets out for a common understanding
of SUD and the assessment methods currently made use of by planners, architects, engineers and surveyors to build environmental
capacity. The paper has done this by:
相似文献
• | fore-grounding the question of urban development and representing the process of urbanisation as a life cycle of inter-related activities; |
• | agreeing the sustainable development issues underlying the urban process; |
• | identifying the environmental, economic and social structure, spatial level and time scales of sustainable urban development. |
134.
Thomas Rausch 《Hydrobiologia》1981,78(3):237-251
The spectrophotometric evaluation of micro-algal protein needs a prior extraction from cells in order to liberate protein for measurement. The conditions of extraction (temperature, duration, normality of sodium hydroxide, pretreatment) which yield optimal protein content are tested with three algal cultures (Scenedesmus, Synechococcus, Asterionella). A standard method of extraction is presented. Comparison of this method with nine published methods reveals markedly lower protein yields for easy extractable (43–100%) and hard extractable (5–75%) algal species, relative to this method, depending on ease of cell wall breakage. The application of this standard method to field investigations is demonstrated and compared to other biochemical parameters. The advantages of this method over other protein extraction methods, with respect to field material, are discussed. 相似文献
135.
Caruthers J Zucker F Worthey E Myler PJ Buckner F Van Voorhuis W Mehlin C Boni E Feist T Luft J Gulde S Lauricella A Kaluzhniy O Anderson L Le Trong I Holmes MA Earnest T Soltis M Hodgson KO Hol WG Merritt EA 《Protein science : a publication of the Protein Society》2005,14(11):2887-2894
We have determined the crystal structures of three homologous proteins from the pathogenic protozoans Leishmania donovani, Leishmania major, and Trypanosoma cruzi. We propose that these proteins represent a new subfamily within the isochorismatase superfamily (CDD classification cd004310). Their overall fold and key active site residues are structurally homologous both to the biochemically well-characterized N-carbamoylsarcosine-amidohydrolase, a cysteine hydrolase, and to the phenazine biosynthesis protein PHZD (isochorismase), an aspartyl hydrolase. All three proteins are annotated as mitochondrial-associated ribonuclease Mar1, based on a previous characterization of the homologous protein from L. tarentolae. This would constitute a new enzymatic activity for this structural superfamily, but this is not strongly supported by the observed structures. In these protozoan proteins, the extended active site is formed by inter-subunit association within a tetramer, which implies a distinct evolutionary history and substrate specificity from the previously characterized members of the isochorismatase superfamily. The characterization of the active site is supported crystallographically by the presence of an unidentified ligand bound at the active site cysteine of the T. cruzi structure. 相似文献
136.
Aim To describe a protocol for incorporating a temporal dimension into historical biogeographical analysis, while maintaining the essential independence of all datasets, involving the generation of general area cladograms. Location Global. Methods General area cladograms (GACs) are a reconstruction of the evolutionary history of a set of areas and unrelated clades within those areas. Nodes on a GAC correspond to speciation events in a group of taxa; general nodes are those at which multiple unrelated clades speciate. We undertake temporal calibration of GACs using molecular clock estimates of splitting events between extant taxa as well as first appearance data from the fossil record. We present two examples based on re‐analysis of previously published data: first, a temporally calibrated GAC generated from secondary Brooks parsimony analysis (BPA) of six extant bird clades from the south‐west of North America using molecular clock estimates of divergence times; and second, an analysis of African Neogene mammals based on a phylogenetic analysis for comparing trees (PACT) analysis. Results A hypothetical example demonstrates how temporal calibration reveals potentially critical information about the timing of both unique and general events, while also illustrating instances of incongruence between dates generated from molecular clock estimates and fossils. For the African Neogene mammal dataset, our analysis reveals that most mammal clades underwent geodispersal associated with the Neogene climatic optimum (c. 16 Ma) and vicariant speciation in central Africa correlated with increased aridity and cooler temperatures around 2.5 Ma. Main conclusions Temporally calibrated GACs are valuable tools for assessing whether coordinated patterns of speciation are associated with large‐scale climatic or tectonic phenomena. 相似文献
137.
Meike T. Wortel Deepa Agashe Susan F. Bailey Claudia Bank Karen Bisschop Thomas Blankers Johannes Cairns Enrico Sandro Colizzi Davide Cusseddu Michael M. Desai Bram van Dijk Martijn Egas Jacintha Ellers Astrid T. Groot David G. Heckel Marcelle L. Johnson Ken Kraaijeveld Joachim Krug Liedewij Laan Michael Lässig Peter A. Lind Jeroen Meijer Luke M. Noble Samir Okasha Paul B. Rainey Daniel E. Rozen Shraddha Shitut Sander J. Tans Olivier Tenaillon Henrique Teotónio J. Arjan G. M. de Visser Marcel E. Visser Renske M. A. Vroomans Gijsbert D. A. Werner Bregje Wertheim Pleuni S. Pennings 《Evolutionary Applications》2023,16(1):3-21
138.
Adrian Favell 《Ethnic and racial studies》2016,39(13):2352-2360
ABSTRACTA critical review of Alba and Foner's Strangers No More (2015) which focuses on questioning their comparative assimilation of European cases of immigrant integration to the North American, specifically U.S., experience. While this may work in terms of how national immigrant integration has mitigated over time racial discrimination for older, post-colonial migrants, it misrepresents the complex differentiations involved in the super-diversity of recent ‘new’ migrations within and to Europe. In particular, the variety of types and origins of recent migration is lost in their understanding of the U.K. case, with problems linked to their interpretation of data about minorities and foreigners in the country. 相似文献
139.
David T. Parkin 《Bird Study》2013,60(3):223-242
Capsule Based on the 1999 Witherby Memorial Lecture – reviews how developments in molecular and population genetics have led to a reappraisal of species limits in birds. The taxonomy of birds of the West Palearctic has moved from the comparative stability of the ‘Voous List’ into a period of serious activity, with new data emerging in almost every issue of every evolutionary and avian journal! This activity comes from two directions. Firstly, developments in population genetics, molecular biology, acoustics, behaviour and distributional studies have opened new avenues to measuring differentiation among groups of birds. This, in turn, has led to the recognition that earlier views of what constitutes a ‘species’ are in need of modification (‘improvement’), and the emergence of the ‘lineage concept’ of species. I review some of the species concepts most relevant to avian studies, and attempt to show how and why this change has happened, and its consequences for taxonomy and species limits. Examples are given in the form of ‘case studies’, and include Carrion/Hooded Crows Corvus corone/cornix, Green-winged/Eurasian Teals Anas carolinensis/crecca and Phylloscopus warblers. 相似文献
140.
Question: How does one best choose native vegetation types and site them in reclamation of disturbed sites ranging from cropland and strip mines? Application: World‐wide, demonstrated in SE Montana. Methods: We assumed that pre‐disturbance native communities are the best targets for revegetation, and that the environmental facet each occupies naturally provides its optimal habitat. Given this assumption, we used pre‐strip‐mine data (800 points from a 88 km2 site) to demonstrate statistical methods for identifying native communities, describing them, and determining their environments. Results and conclusions: Classification and pruning analysis provided an objective method for choosing the number of target community types to be used in reclamation. The composition of eight target types, identified with these analyses, was described with a relevé table to provide a species list, target cover levels and support the choice of species to be seeded. As a basis for siting communities, we modeled community presence as a function of topography, slope/aspect, and substrate. Logistic GLMs identified the optimal environment for each community. Classification and Regression Tree (CART) analysis identified the most probable community in each environmental facet. Topography and slope were generally the best predictors in these models. Because our analyses relate native vegetation to undisturbed environments, our results may apply best to sites with minimal substrate disturbance (i.e. better to abandoned cropland than to strip‐mined sites). 相似文献