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排序方式: 共有153条查询结果,搜索用时 15 毫秒
31.
Qiliqiangxin inhibits angiotensin II‐induced transdifferentiation of rat cardiac fibroblasts through suppressing interleukin‐6 下载免费PDF全文
32.
We have reported that vascular endothelial growth factor (VEGF)-A induces the proliferation of human pulmonary valve endothelial cells (HPVECs) through nuclear factor in activated T cells (NFAT)c1 activation [1]. Here we show that VEGF-A increases the migration of HPVECs through NFATc1 activation, suggesting that VEGF-A/NFATc1 regulates the migration of HPVECs. To learn how this pathway may be involved in post-natal valvular repair, HPVECs were treated with VEGF-A, with or without cyclosporine A to selectively block VEGF-NFATc1 signaling. Down Syndrome critical region 1 (DSCR1) and heparin-binding EGF-like growth factor (HB-EGF) are two genes identified by DNA microarray as being up-regulated by VEGF-A in a cyclosporine-A-sensitive manner. DSCR1 silencing increased the migration of ovine valve endothelial cells, whereas HB-EGF silencing inhibited migration. This differential effect suggests that VEGF-A/NFATc1 signaling might be a crucial coordinator of endothelial cell migration in post-natal valves. 相似文献
33.
p~(38)MAPK在IL-18诱导肾小管上皮细胞转分化中的作用 总被引:1,自引:0,他引:1
目的:白细胞介素18(IL-18)可诱导肾小管上皮细胞转分化,本研究探讨其是否是通过p38MAPK途径而起作用。方法:应用不同浓度的p38MAPK通路特异性阻断剂SB203580(0、5、10、20μmol/L)预孵育人近端肾小管上皮细胞(HK-2细胞)30min后,加入IL-18(100ng/ml)共培养24、48、72h。应用RT-PCR法检测α-平滑肌肌动蛋白(α-SMA)mRNA的表达水平;应用ELISA法测定细胞浆中α-SMA蛋白质含量。结果:SB203580呈剂量依赖性地抑制IL-18诱导的HK-2细胞α-SMA基因表达(P0.05)。结论:p38MAPK通路是调控IL-18诱导肾小管上皮细胞转分化的主要信号通路之一。 相似文献
34.
Cell plasticity is a central issue in stem cell biology. In many recent discussions, observation of cell fusion has been seen
as a confounding factor which calls into question published results concerning cell plasticity of, particularly, adult stem
cells. An examination of the voluminous literature of "somatic cell fusion" suggests the relatively frequent occurrence of
"spontaneous" cell fusion and shows that the complicated cellular phenotypes which it can give rise to have long been recognized.
Here, a brief overview of this field is presented, with emphasis on studies of special relevance to current work on cell plasticity.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
35.
Bonnet D 《Birth defects research. Part C, Embryo today : reviews》2003,69(3):219-229
Considerable effort has been made in recent years in defining the embryonic origin of the hematopoietic stem cell (HSC). Using transgenic mouse models, a number of genes that regulate the formation, self-renewal, or differentiation of HSCs have been identified. Of particular interest, it has recently been shown that key regulators of definitive blood formation played a crucial role in adult HSC development. Specifically, the use of some of these regulatory molecules has dramatically improved the potential of adult HSC expansion. Furthermore, the elucidation of the molecular phenotype of the HSC has just begun. Finally, unexpected degrees of HSC developmental or differentiation plasticity have emerged. In this review, we will summarize the recent advances made in the human HSC field, and we will examine the impacts these discoveries may have clinically and on our understanding of the organization of the human hematopoietic system. 相似文献
36.
Peggy Wallace John R. Kennedy Joseph Mendicino 《In vitro cellular & developmental biology. Animal》1994,30(3):168-180
Summary The morphologic and functional properties of explant out-growth cells and epithelial cells isolated from swine trachea epithelium
by proteolysis were examined. A mixed population of ciliated, serous, and basal cells, obtained from out-growths, from proteolysis
of trachea epithelium, and from unattached explants in organ culture, all yielded cell cultures that were composed almost
entirely of mucus-secreting cells. When the cells were grown in primary or secondary culture on a modified collagen matrix
in supplemented HAM:DMEM (1:1) medium they expressed a mucus-secreting phenotype with numerous mucus granules at various stages
of maturation and incorporated [3H]GlcN and35SO4 into secreted mucin glycoproteins. Results obtained in these studies suggest that extensive transdifferentiation of ciliated
and serous cells to mucus-secreting cells occurs after the release and during subsequent attachment and culture. Ciliated
cells containing mucus granules were seen in various stages of cilia resorption. Basal cells containing mucus granules were
also frequently observed. The number of mucus-secreting cells and the synthesis of mucin glycoproteins increased dramatically
with time of attachment and culture, whereas cell proliferation, population doubling time of 72 h, and incorporation of [3H]thymidine into DNA increased much more slowly. The number of mucus-secreting cells correlated closely with the level of
secretion of mucin glycoproteins. Taken collectively, these studies help to elucidate the transdifferentiation process, which
dramatically increases the number of mucus-secreting cells after disruption and release of epithelial cells from swine tracheobronchial
epithelium. A similar mechanism involving disruption of the extracellular matrix may be involved in the stimulation of hypersecretion
of mucus and mucin glycoproteins by chemical and infections irritants. 相似文献
37.
The molecular basis of transdifferentiation 总被引:1,自引:0,他引:1
Li WC Yu WY Quinlan JM Burke ZD Tosh D 《Journal of cellular and molecular medicine》2005,9(3):569-582
38.
Transgenic expression of FGF8 and FGF10 induces transdifferentiation of pancreatic islet cells into hepatocytes and exocrine cells 总被引:3,自引:0,他引:3
Yamaoka T Yoshino K Yamada T Yano M Matsui T Yamaguchi T Moritani M Hata J Noji S Itakura M 《Biochemical and biophysical research communications》2002,292(1):138-143
FGF signaling is essential for normal development of pancreatic islets. To examine the effects of overexpressed FGF8 and FGF10 on pancreatic development, we generated FGF8- and FGF10-transgenic mice (Tg mice) under the control of the glucagon promoter. In FGF8-Tg mice, hepatocyte-like cells were observed in the periphery of pancreatic islets, but areas of alpha and beta cells did not decrease, whereas in FGF10-Tg mice, pancreatic ductal and acinar cells were found in islets, concomitantly with disturbed beta-cell differentiation. These results suggest that FGF8 and FGF10 play important roles in development of hepatocytes and exocrine cells, respectively, and explain the absence of FGF8 expression in normal islets and pancreatic hypoplasia in FGF10-deficient mice. 相似文献
39.
40.