ELF exposure facility for human testing.

A laboratory facility specifically designed for controlled human exposure to 60-Hz electric (0 to 16 kV/m) and magnetic (0 to 32 A/m, B = 0 to 40 microT) fields has been constructed. The facility presents uniform fields under controlled temperature and humidity. Special control systems allow collection of physiological data during, as well as before and after, exposure to electric fields at strengths to 16 kV/m under verified double-blind control. Exposure to continuous or intermittent fields is possible in the facility. The capability of obtaining physiological data during actual exposure to constant or intermittent, 60-Hz fields, and of doing so without either the subject or the experimenter being aware of actual field conditions, is a critical factor in valid experimentation.     

Applying structure-from-motion habitat reconstruction and GIS terrain analysis to test hypotheses about nest-site selection by shorebirds

Habitat variables related to vegetation type and structure are routinely identified as important components of nest-site selection for birds. For ground-nesting birds, small-scale (< 0.5 m) microtopography may also play a role in nest-site selection through its effects on nest concealment and microclimate. Manual measurements of microtopography are challenging, time-consuming, and subject to user error. Ultrahigh-density point clouds generated using structure-from-motion (SfM) algorithms and photomosaics captured during Unmanned Aerial Vehicle (UAV) surveys can potentially provide detailed topographical measurements with less error. We used multiple indices of surface roughness to examine the effect of microtopography on nest site selection by White-rumped Sandpipers (Calidris fuscicollis) at East Bay Migratory Bird Sanctuary, Nunavut, Canada. We measured microtopography manually using the relative height index within a 1-m radius of used and unused sites. We generated three digital indices of surface roughness for 1-m and 5-m scales around nests and unused sites, including standard deviation of slope, slope variability, and 2D:3D area ratio. We compared the digital indices of used and unused sites at both spatial scales. Relative height of nearby hummocks was significantly lower at nests than at unused sites, indicating selection for flatter sites. Similarly, two of three digitally calculated terrain roughness indices were significantly lower at nests than unused sites at both the 1-m and 5-m scales. The 2D:3D ratio did not differ between nests and unused sites. Manual and UAV-derived measures were significantly correlated, but with substantial unexplained variation (Pearson’s r = 0.35–0.46). Our results suggest that White-rumped Sandpipers select nest sites in areas with low terrain roughness, potentially to increase their field of view to monitor approaching predators. We also demonstrate the applicability of SfM habitat reconstruction for testing hypotheses of small-scale habitat variables that may be impossible to study using traditional methods.     

Pudendal and median nerve sensory perception threshold: A comparison between normative studies

Background: For the evaluation of sensory innervation, normative data are necessary as a comparison.

Aims: To compare our current perception thresholds (CPTs) with normative data from other research.

Methods: Healthy volunteers were assessed for 2000, 250, and 5?Hz CPTs of the median and pudendal nerve and data were compared with other studies.

Results: Normative data in the studied group n?=?41 (male: 21; female: 20) for the median nerve, 2?kHz, 250?Hz, and 5?Hz were respectively: 241.85?±?67.72 (140–444); 106.27?±?39.12 (45–229); 82.05?±?43.40 (13–271). Pudendal nerve CPTs 250?Hz were: 126.44?±?69.46 (6–333). For men 2?kHz: 349.95?±?125.76 (100–588); 5?Hz: 132.67?±?51.81 (59–249) and women 2?kHz: 226.20?±?119.65 (64–528); 5?Hz: 92.45?±?44.66 (35–215). For the median nerve no statistical differences for gender were shown. For the pudendal nerve, only 250?Hz showed no difference for gender (t-test: 0.516). Comparison of our data with CPTs of other normative data showed no agreement for the pudendal nerve. For the median nerve only 2?kHz showed agreement in three studies and for 5?Hz with one study.

Conclusion: Comparing normative data of multiple studies shows a variety of results and poor agreement. Therefore, referring to normative data of other studies should be handled with caution.     

The interpretation of intracellular measurements of membrane potential,resistance, and coupling in cells of higher plants

Results of microelectrode impalements of parenchymal cells of coleoptiles made in several different laboratories differ widely. The highest membrane potentials correlate with lower input resistance and the presence of intercellular coupling, whereas high input resistance seems to be associated with an absence of measurable coupling and possibly lower membrane potentials. In this paper we demonstrate that these results are consistent with (1) a tonoplast resistance several times greater than the input resistance of the cytoplasmic compartment, and (2) the presence of variable amounts of shunting introduced by insertion of the microelectrode through the cell membranes. The general consequences of this hypothesis are developed quantitatively. If the ideas are applicable to other tissues of higher plants-and on this point the evidence is still insufficient to judgeboth the design of experiments and the interpretation of measurements made with microelectrodes will have to be reevaluated.     

Integrated Life Cycle Assessment and Life Cycle Cost Model for Comparing Plug‐in versus Wireless Charging for an Electric Bus System

An integrated life cycle assessment and life cycle cost (LCC) model was developed to compare the life cycle performance of plug‐in charging versus wireless charging for an electric bus system. The model was based on a bus system simulation using existing transit bus routes in the Ann Arbor–Ypsilanti metro area in Michigan. The objective is to evaluate the LCCs for an all‐electric bus system utilizing either plug‐in or wireless charging and also compare these costs to both conventional pure diesel and hybrid bus systems. Despite a higher initial infrastructure investment for off‐board wireless chargers deployed across the service region, the wireless charging bus system has the lowest LCC of US$0.99 per bus‐kilometer among the four systems and has the potential to reduce use‐phase carbon emissions attributable to the lightweighting benefits of on‐board battery downsizing compared to plug‐in charging. Further uncertainty analysis and sensitivity analysis indicate that the unit price of battery pack and day or night electricity price are key parameters in differentiating the LCCs between plug‐in and wireless charging. Additionally, scenario analyses on battery recycling, carbon emission pricing, and discount rates were conducted to further analyze and compare their respective life cycle performance.     

A Reassessment of Prebiotic Organic Synthesis in Neutral Planetary Atmospheres

The action of an electric discharge on reduced gas mixtures such as H₂O, CH₄ and NH₃ (or N₂) results in the production of several biologically important organic compounds including amino acids. However, it is now generally held that the early Earth’s atmosphere was likely not reducing, but was dominated by N₂ and CO₂. The synthesis of organic compounds by the action of electric discharges on neutral gas mixtures has been shown to be much less efficient. We show here that contrary to previous reports, significant amounts of amino acids are produced from neutral gas mixtures. The low yields previously reported appear to be the outcome of oxidation of the organic compounds during hydrolytic workup by nitrite and nitrate produced in the reactions. The yield of amino acids is greatly increased when oxidation inhibitors, such as ferrous iron, are added prior to hydrolysis. Organic synthesis from neutral atmospheres may have depended on the oceanic availability of oxidation inhibitors as well as on the nature of the primitive atmosphere itself. The results reported here suggest that endogenous synthesis from neutral atmospheres may be more important than previously thought. Stanley L. Miller died May 20, 2007.     

Structure and assembly of type VI secretion system cargo delivery vehicle

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Influence of functional unit on the life cycle assessment of traction batteries

Goal, Scope and Background This paper describes the influence of the choice of the functional unit on the results of an environmental assessment of different battery technologies for electric and hybrid vehicles. Battery, hybrid and fuel cell electric vehicles are considered as being environmentally friendly. However, the batteries they use are sometimes said to be environmentally unfriendly. At the current state of technology different battery types can be envisaged: lead-acid, nickel-cadmium, nickel-metal hydride, lithium-ion and sodium-nickel chloride. The environmental impacts described in this paper are based on a life cycle assessment (LCA) approach. One of the first critical stages of LCA is the definition of an appropriate and specific functional unit for electric and hybrid vehicle application. Most of the known LCA studies concerning batteries were performed while choosing different functional units, although this choice can influence the final results. An adequate functional unit, allowing to compare battery technologies in their real life vehicle application should be chosen. The results of the LCA are important as they will be used as a decision support for the end-of-life vehicles directive 2000/53/EC (Official Journal of the European Communities L269/24 2000). As a consequence, a thorough analysis is required to define an appropriate functional unit for the assessment of batteries for electric vehicles. This paper discusses this issue and will mainly focus on traction batteries for electric vehicles. Main Features An overview of the different parameters to be considered in the definition of a functional unit to compare battery technologies for battery electric vehicle application is described and discussed. An LCA study is performed for the most relevant potential functional units. SimaPro 6 is used as a software tool and Eco-indicator 99 as an impact assessment method. The influence of the different selected functional units on the results (Eco-indicator Points) is discussed. The environmental impact of the different electric vehicle battery technologies is described. A sensitivity analysis illustrates the robustness of the obtained results. Results and Discussion Five main parameters are considered in each investigated functional unit: an equal depth of discharge is assumed, a relative number of batteries required during the life of the vehicle is calculated, the energy losses in the battery and the additional vehicle consumption due to the battery mass is included and the same lifetime distance target is taken into account. On the basis of the energy content, battery mass, number of cycles and vehicle autonomy three suitable functional units are defined: ‘battery packs with an identical mass’, ‘battery packs with an identical energy content’ and ‘battery packs with an identical one-charge range’. The results show that the differences in the results between these three functional units are small and imply less variation on the results than the other uncertainties inherent to LCA studies. On the other hand, the results obtained using other, less adequate, functional units can be quite different. Conclusions When performing an LCA study, it’s important to choose an appropriate functional unit. Most of the time, this choice is unambiguous. However, sometimes this choice is more complicated when different correlated parameters have to be considered, as it is the case for traction batteries. When using a realistic functional unit, the result is not influenced significantly by the choice of one out of the three suitable functional units. Additionally, the life cycle assessment allowed concluding that three electric vehicle battery technologies have a comparable environmental impact: lead-acid, nickel-cadmium and nickel-metal hydride. Lithium-ion and sodium-nickel chloride have lower environmental impacts than the three previously cited technologies when used in a typical battery electric vehicle application. Recommendations and Perspectives The article describes the need to consider all relevant parameters for the choice of a functional unit for an electric vehicle battery, as this choice can influence the conclusions. A more standardised method to define the functional unit could avoid these differences and could make it possible to compare the results of different traction battery LCA studies more easily.     

延髓腹外侧Btzinger复合体呼吸时相转换效应的研究

实验在４０只氨基甲酸乙酯麻醉的成年家兔上进行，观察电刺激Ｂ¨ｏｔｚｉｎｇｅｒ复合体（Ｂ¨ｏｔＣ）对呼吸节律的影响。结果表明：（１）吸气相早期短串电刺激Ｂ¨ｏｔＣ导致膈神经放电被短暂抑制，被抑制的程度与刺激强度呈正相关。当刺激落位在吸气相中、晚期时，可导致吸气提前终止（“吸气切断”），之后跟随一缩短的呼气相。导致“吸气切断”的阈强度与刺激落位呈负相关。（２）呼气相短串电刺激Ｂ¨ｏｔＣ，可诱导膈神经短暂放电，呼气相晚期诱导出现放电之后往往跟随一正常的吸气放电，结果导致该呼气相缩短。该效应亦具有刺激强度、刺激落位依赖性。结果提示：Ｂ¨ｏｔＣ参与吸气向呼气以及呼气向吸气的时相转换。     

Development of a sexual dimorphism in a central pattern generator driving a rhythmic behavior: The role of glia‐mediated potassium buffering in the pacemaker nucleus of the weakly electric fish Apteronotus leptorhynchus

Central pattern generators play a critical role in the neural control of rhythmic behaviors. One of their characteristic features is the ability to modulate the oscillatory output. An important yet little‐studied type of modulation involves the generation of oscillations that are sexually dimorphic in frequency. In the weakly electric fish Apteronotus leptorhynchus, the pacemaker nucleus serves as a central pattern generator that drives the electric organ discharge of the fish in a one‐to‐one fashion. Males discharge at higher frequencies than females—a sexual dimorphism that develops under the influence of steroid hormones. The two principal neurons that constitute the oscillatory network of the pacemaker nucleus are the pacemaker and relay cells. Whereas the number and size of the pacemaker and relay cells are sexually monomorphic, pronounced sex‐dependent differences exist in the morphology, and subcellular properties of astrocytes, which form a syncytium closely associated with these neurons. In females, compared to males, the astrocytic syncytium covers a larger area surrounding the pacemaker and relay cells and exhibits higher levels of expression of connexin‐43 expression. The latter indicates a strong gap‐junction coupling of the individual cells within the syncytium. It is hypothesized that these sex‐specific differences result in an increased capacity for buffering of extracellular potassium ions, thereby lowering the potassium equilibrium potential, which, in turn, leads to a decrease in the oscillation frequency. This hypothesis has received strong support from simulations based on computational models of individual neurons and the whole neural network of the pacemaker nucleus.