吐鲁番市胃食管反流病发生相关危险因素分析（附280例报道）

目的：探讨280例胃食管反流病（GERD）的分布特点及危险因素。方法：对临床诊断和胃镜确诊的280例GERD患者进行临床和风险因子相关性分析。结果：不论汉族还是维族,男性患者比例均明显高于女性;汉族患者高发年龄段早于维族患者（z=-2．939,P=0．003,）;汉族和维族患者占反流性食管炎和Barrett食管比例分别为42．4％、81_3％及56．5％、18．8％,其中汉族患者Barrett食管比例较高（X2=14．358,P=0．000）;肥胖、习惯性便秘、重体力活动者、饮食习惯不良在维族患者中的比例较高（P〈0．001）。结论：GERD与性别、年龄密切相关,男性多于女性,汉族患者发病年龄高峰旱于维族患者;汉族患者Barrett食管发生比例高于维族患者;肥胖、习惯性便秘、重体力活动、饮食习惯不良可能是GERD尤其是维族人群GERD的危险因素。     

变应性鼻炎与IRF5的单核苷酸多态性关系

目的:探讨变应性鼻炎与干扰素调节因子5(IRFS)的单核苷酸多态性(SNP)的相关性.方法:采用聚合酶链(PCR)和限制性片段长度多态性(RFL P)方法在新加坡华人中检测110例变应性鼻炎患者及101健康对照组IRF5位点上的3个单核苷酸多态性(rs4728142,rs11770589,rs2280714)计算基因型和等位基因频率.结果:IRF5基因的3个位点的基因型和等位基因频率在变应性鼻组和对照组间差异无统计学意义.结论:新加坡华人IRF5 rs4728142,rsl1770589,rs2280714多态性与变应性鼻炎无明显相关性.     

Lysine maintenance requirement and efficiency of its utilisation in young pigs as estimated by comparative slaughter technique

An experiment was carried out on weaner pigs (initial BW 10.8 kg) to estimate the maintenance requirement for lysine (Lys) and its marginal efficiency of utilisation using a comparative slaughter technique. Three groups of six pigs each were fed purified diets for 21 days supplying Lys at 19.5, 78 or 195 mg/kg W^0.75, which corresponded to 50, 200 or 500% of the assumed maintenance requirement. All other essential amino acids were given at 50% excess. At the end of the experiment, pigs were killed for whole-body nitrogen (N) and amino acid analysis. A representative group of six pigs was analysed at the beginning of the experiment. Based on regression equations, relating Lys or N retention to Lys intake, Lys requirement for zero Lys retention was estimated to be 121 mg/kg W^0.75, while Lys requirement corresponding to zero N retention was 41.7 mg/kg W^0.75. At N equilibrium, the pigs lost 65 mg of Lys per kg W^0.75 daily while at zero Lys retention, the daily N retention was 156 mg/kg W^0.75. The marginal efficiency of lysine utilisation was 0.91. It is concluded that zero lysine retention is a better criterion of lysine maintenance requirement than zero N retention.     

Clinical significance of serum transforming growth factor-β1 in lung cancer

Background: Transforming growth factor-β1 (TGF-β1) plays a critical role in human cancer development. Present study aimed to explore the clinical significance of serum TGF-β1 levels in patients with lung cancer and analyze the relationship between TGF-β1 and existing tumor markers for lung cancer. Methods: Serum was collected from 118 patients with lung cancer and 40 healthy volunteers. Serum TGF-β1 levels were measured by enzyme-linked immunosorbent assay (ELISA), and the association with various clinical characteristics was analyzed. The diagnostic value of TGF-β1 was assessed alone and in combination with existing tumor markers for lung cancer. Results: Serum TGF-β1 levels were significantly higher in patients with lung cancer compared to healthy volunteers [0.6 × 10⁵ (0.4 × 10⁵, 0.9 × 10⁵) pg/ml vs 0.5 × 10⁵ (0.3 × 10⁵, 0.7 × 10⁵) pg/ml, P = 0.040]. Although there was a positive correlation between serum TGF-β1 levels and advanced stages, the significant difference was not found between early stages and advanced stages (P = 0.116). The ability of serum TGF-β1 to discriminate lung cancer at a cutoff value of 79,168 pg/ml exhibited sensitivity of 30.6% and specificity of 97.5%. Serum TGF-β1 levels were correlated to cytokeratin fragment 21-1 (CYFRA21-1; R = 0.308, P = 0.020) and neuron-specific enolase (NSE; R = 0.558, P = 0.003). The diagnostic accuracy rates for the existing lung-tumor markers, as SCC, CYFRA21-1, and NSE, were increased from 20.0%, 34.6%, and 45.9% to 48.9%, 51.7%, and 54.5%, respectively by the inclusion of serum TGF-β1 levels. Conclusion: Quantification of serum TGF-β1 levels by ELISA may provide a novel complementary tool for the clinical diagnosis of lung cancer.     

Transcription of the human microsomal epoxide hydrolase gene (EPHX1) is regulated by an HNF-4α/CAR/RXR/PSF complex

Microsomal epoxide hydrolase (mEH) is a bifunctional protein that plays a central role in the metabolism of numerous xenobiotics as well as mediating the sodium-dependent transport of bile acids into hepatocytes where they are involved in cholesterol excretion and metabolism, lipid digestion and regulating numerous signaling pathways. Previous studies have demonstrated the critical role of GATA-4 and a C/EBPα–NF/Y complex in the regulation of the mEH gene (EPHX1). In this study we show that HNF-4α and CAR/RXR also bind to the proximal promoter region and regulate EPHX1 expression. Bile acids, which inhibit the expression of HNF-4α also decrease the expression of EPHX1. Studies also established that the binding of HNF-4α was essential for the activation of EPHX1 activity by CAR suggesting the formation of a complex between these adjacent factors. The nature of this regulatory complex was further explored using a biotinylated oligonucleotide of this region in conjunction with BioMag beads and mass spectrometric analysis which demonstrated the presence of an additional inhibitory factor (PSF), confirmed by co-immunoprecipitation and ChIP analyses, which interacted with DNA-bound CAR/RXR/HNF-4α forming a 4-component regulatory complex.     

Neuron-restrictive silencer factor functions to suppress Sp1-mediated transactivation of human secretin receptor gene

In the present study, a functional neuron restrictive silencer element (NRSE) was initially identified in the 5′ flanking region (− 83 to − 67, relative to ATG) of human secretin receptor (hSCTR) gene by promoter assays coupled with scanning mutation analyses. The interaction of neuron restrictive silencer factor (NRSF) with this motif was later indicated via gel mobility shift and ChIP assays. The silencing activity of NRSF was confirmed by over-expression and also by shRNA knock-down of endogenous NRSF. These studies showed an inverse relationship between the expression levels of NRSF and hSCTR in the cells. As hSCTR gene was previously shown to be controlled by two GC-boxes which are regulated by the ratio of Sp1 to Sp3, in the present study, the functional interactions of NRSF and Sp proteins to regulate hSCTR gene was investigated. By co-immunoprecipitation assays, we found that NRSF could be co-precipitated with Sp1 as well as Sp3 in PANC-1 cells. Interestingly, co-expressions of these factors showed that NRSF could suppress Sp1-mediated, but not Sp3-mediated, transactivation of hSCTR. Taken together, we propose here that the down-regulatory effects of NRSF on hSCTR gene expression are mediated via its suppression on Sp1-mediated transactivation.     

创伤弧菌毒力相关因子的全基因组关联分析研究

【目的】创伤弧菌是致死率最高的弧菌物种,但目前尚无在全基因组层面挖掘毒力相关因子的研究。本研究以创伤弧菌分离来源(临床和环境)作为不同表型,通过与260株基因组序列进行关联分析,挖掘毒力相关因子,从而进一步了解创伤弧菌致病因素。【方法】对139株创伤弧菌分离株进行高通量测序,获取其全基因组序列;与公共数据库已公开发表的121株基因组整合,使用pyseer软件进行全基因组关联分析,对与不同分离来源显著相关的基因进行注释和解读。【结果】共发现11个基因与临床分离株显著相关,其中9个是本研究新发现的创伤弧菌潜在毒力相关因子。【结论】本研究使用群体基因组学和统计遗传学方法,在全基因组范围扫描挖掘了创伤弧菌毒力相关因子,为深入揭示该物种致病机制、设计新的疫苗和治疗靶点提供了重要依据。     

Impact of feed restriction on the performance of highly prolific lactating sows and its effect on the subsequent lactation

A total of 50 mixed parity sows of a high-prolificacy genetic line were used to evaluate the impact of feed restriction during lactation on their production and reproductive performance and their performance in the subsequent lactation. From day 7 of lactation, sows were distributed according to a completely randomized experimental design into two treatments. In treatment 1, sows were fed 8.0 kg feed/day (control) and in treatment 2, sows were fed 4.0 kg/day. The same suckling pressure was maintained until weaning on day 28 of lactation. Average minimum and maximum temperatures measured during the experimental period were 32.1°C and 16.5°C, respectively. Control sows presented significantly higher feed intake (P<0.001) compared with the restricted sows (6.43 v. 4.14 kg/day, respectively). Treatments influenced BW and backfat thickness losses (P<0.001). Control sows lost less BW than the restricted-fed sows (7.8 v. 28.2 kg). Restricted-fed sows lost more backfat thickness than those in the control group (3.97 v. 2.07 mm; P<0.01). Restricted-fed sows tended (P<0.10) to be lighter at weaning compared with the control sows (211 v. 227 kg). The composition of BW loss was influenced by the treatments (P<0.001), as the restricted-fed sows lost more body protein, lipids and energy compared with the control sows (3.90 v. 0.98 kg, 11.78 v. 4.83 kg and 584 v. 224 MJ, respectively). Litter weight gain was greater (P<0.05) in control sows than in restricted-fed sows (2.70 v. 2.43 kg/day). Daily milk production was 19% higher (P<0.01) in the control sows compared with the restricted-fed sows (8.33 v. 6.99 kg/day). However, restricted-fed sows presented a higher (P<0.05) lactation efficiency than the sows of the control group (82.30% v. 72.93%). No differences were detected (P>0.10) in weaning-to-estrus interval and averaged 4.3 days. No effect of the treatment (P>0.10) was observed on any of the studied performance traits in the subsequent lactation, except for litter size at birth that tended (15.2 v. 14.1; P<0.10) to be lower for the restricted sows. In conclusion, the present study demonstrated that feed restriction during lactation leads to intense catabolism of the body tissues of sows, negatively affecting their milk production, and the litter weight gain and possibly number of piglets born in the next litter. On the other hand, restricted-fed sows are more efficient, producing more milk per amount of feed intake.