全文获取类型
收费全文 | 7476篇 |
免费 | 885篇 |
国内免费 | 739篇 |
出版年
2024年 | 19篇 |
2023年 | 155篇 |
2022年 | 160篇 |
2021年 | 229篇 |
2020年 | 297篇 |
2019年 | 342篇 |
2018年 | 373篇 |
2017年 | 325篇 |
2016年 | 304篇 |
2015年 | 291篇 |
2014年 | 381篇 |
2013年 | 692篇 |
2012年 | 251篇 |
2011年 | 360篇 |
2010年 | 282篇 |
2009年 | 420篇 |
2008年 | 398篇 |
2007年 | 378篇 |
2006年 | 378篇 |
2005年 | 351篇 |
2004年 | 315篇 |
2003年 | 296篇 |
2002年 | 270篇 |
2001年 | 208篇 |
2000年 | 142篇 |
1999年 | 189篇 |
1998年 | 167篇 |
1997年 | 143篇 |
1996年 | 120篇 |
1995年 | 119篇 |
1994年 | 104篇 |
1993年 | 86篇 |
1992年 | 86篇 |
1991年 | 72篇 |
1990年 | 52篇 |
1989年 | 26篇 |
1988年 | 40篇 |
1987年 | 34篇 |
1986年 | 31篇 |
1985年 | 30篇 |
1984年 | 46篇 |
1983年 | 26篇 |
1982年 | 45篇 |
1981年 | 20篇 |
1980年 | 13篇 |
1979年 | 15篇 |
1978年 | 6篇 |
1977年 | 6篇 |
1976年 | 2篇 |
1973年 | 4篇 |
排序方式: 共有9100条查询结果,搜索用时 15 毫秒
51.
We address the question of the long term coexistence of three interacting species whose dynamics are governed by the ordinary differential equations x
i
= X
i
f
i
(i = 1, 2, 3). In order for any theory in this area to be useful in practice, it must utilize as little information as possible concerning the forms of the f
i
, in view of the great difficulty of determining these experimentally. Here we obtain, under rather general conditions on the equations, a criterion for judging whether the species will coexist in a biologically realistic manner. This criterion depends only on the behaviour near the one or two species equilibria of the two dimensional subsystems, the behaviour there being relatively easy to examine experimentally. We show that with the exception of one class of cases, which is a generalization of a classical example of May and Leonard [21], invasibility at each such equilibrium suitably interpreted is both necessary and sufficient for a strong form of coexistence to hold. In the exceptional case, a single additional condition at the equilibria is enough to ensure coexistence. 相似文献
52.
The response of a harpacticoid copepod community to sediment disturbance in a semi-enclosed lagoon 总被引:1,自引:1,他引:0
The harpacticoid fauna of a sandy sediment in the Sluice Dock of Ostend, Belgium, was dominated by a single species, Canuella perplexa, that attained an average annual density of over 400 ind. 10 cm–2 in 1976 and 1977, whereas other species had a total mean density of less than 10 ind. 10 cm–2. In the summer of 1979 a dystrophic crisis with abundant growth of Ulva occurred and in March 1980 the lagoon was drained. After refilling of the lagoon, the sediment was immediately colonized by good swimming harpacticoids, that attained densities of 200 ind. 10 cm–2. Three months after refilling Canuella perplexa reappeared and the system returned to its original state within one month. 相似文献
53.
Phytoplankton community structure and distribution in the nearshore zone of Lake Ontario 总被引:1,自引:1,他引:0
Spatial and temporal distributions of phytoplankton were studied during 1979 in the nearshore zone of Lake Ontario. Genera such as Dinobryon, Oscillatoria, and Gymnodinium tended to be found at discrete depths whereas other genera such as Cryptomonas and Rhodomonas tended to be more uniformly distributed throughout the water column. The discrete depth distribution of some of these genera compared with the weak thermal stabilization of the area suggests depth selection and habitat preference within the algal community.Physical processes probably played a major role in regulating the timing and duration of vertical diatom pulses, and resuspension from the sediment played a major role as to the timing of the autumn growth. Vertical mixing in the nearshore zone was sufficient for some diatom genera such as Tabellaria to maintain populations throughout the summer. These mixing processes, however, affect the stability of community structures and associations that develop within the water column. The role of physical processes and their potential effects on algal production in the Great Lakes is discussed. 相似文献
54.
Comparative studies of diurnal variations of nitrate reductase activity in wheat, oat and barley 总被引:1,自引:0,他引:1
Diurnal variations of in vitro and in vivo (intact tissue assay) nitrate reductase (EC 1.6.6.1) activity and stability were examined in leaves of wheat ( Triticum aestivum L. cv. Runar), oat ( Avcna saliva L. cv. Mustang) and barley ( Hordeum vulgure L. cv. Agneta and cv. Gunillu). Nitrate reductase activity was generally higher for wheat than for oat and barley. However, the diurnal variations of nitrate reductase activity and stability were principally the same for all species, e.g. the high activity during the photoperiod was associated with low stability. All species showed a rapid (30-60 min) increase in the in vitro and in vivo activity when the light was switched on. When light was switched off the in vitro activity decreased rapidly whereas decrease in in vivo activity was slower. These experiments support the hypothesis that an activation/ deactivation mechanism is involved in the regulation of diurnal variations in nitrate reductase activity. Red light enhanced nitrate reductase activity in etiolated wheat and barley leaves. In green leaves, however, the daily increase in nitrate reductase activity was not induced by a brief red light treatment. Indications of different regulation mechanisms for the diurnal variations of nitrate reductase activity among the cereals were not found. 相似文献
55.
Fifteen common native British plants were each sampled at a range of sites in Great Britain and green tissues analysed for several inorganic nutrients. Sampling criteria are discussed. The inter-site variation of each element within a species is assessed as a frequency distribution of raw data. Sample values of parameters including arithmetic mean, variance (coefficient of variation), skewness and kurtosis are presented. Their stability is assessed from nitrogen in sub-samples of Pteridium. This suggested sample sizes were adequate but some distributions had sufficient kurtosis to affect the variance. These parameters showed distinctions between macro- and micro-elements and between species. Some mean values sharply distinguished between species and may help to assess current theories of strategy and adaptation but a wider range of species is needed to clarify trends. Coefficients of variation are discussed and were relatively low for a nation-wide survey after allowing for sampling constraints. Coefficients of skewness and kurtosis showed two-thirds of the sample distributions were non-normal. Ecological aspects of the distributions are discussed and are relevant to studies along environmental gradients. Published hypotheses linking positive skewness to stress in the field are considered and two other postulates discussed. Distribution bounds such as those confining 95% of the values are discussed in relation to possible critical levels of nutrients.Nomenclature follows Clapham et al. (1981), Excursion flora of the British Isles. 3rd ed. University Press, Cambridge, except Chamaenerion. 相似文献
56.
57.
S.M. Loh A. Raya D.S. Cramb L.E. O'Gorman R.A. Skurray 《FEMS microbiology letters》1986,37(2):179-182
Abstract The leading region of the F plasmid has been found to extend the maintenance of the normally unstable plasmid vector pACYC184. This ability is due to effective partitioning of plasmid molecules at cell division. Cloning, deletion analysis and transposon mutagenesis have located the partitioning region (ParL) to be encoded within 63.65–64.11F. Complementation studies indicated that parL is a cis -acting locus. 相似文献
58.
Abstract Three proteins from Halobacterium marismortui , malate dehydrogenase (hMDH), glutamate dehydrogenase (hGDH) and ferredoxin (hFD) were purified and characterized with respect to their molecular masses, amino acid composition and, for hFD only, primary structure. Striking features of halophilic proteins are: the high excess of acidic over basic residues; acidic clusters in the sequence. Low-salt concentration causes inactivation and changes in structural parameters of hMDH and hGDH. Reactivation of hMDH involves long-lived stable intermediates. The salt concentration optimum of enzymic activity is independent of salt nature. The high capacity of halophilic proteins to retain water and salt is due to unique molecular properties, studied by physico-chemical techniques. 相似文献
59.
Mutant forms of staphylococcal nuclease with altered patterns of guanidine hydrochloride and urea denaturation 总被引:19,自引:0,他引:19
Eleven mutant forms of staphylococcal nuclease with one or more defined amino acid substitutions have been analyzed by solvent denaturation by using intrinsic fluorescence to follow the denaturation reaction. On the basis of patterns observed in the value of m--the rate of change of log Kapp (the apparent equilibrium constant between the native and denatured states) with denaturant concentration--these proteins can be grouped into two classes. For class I mutants, the value of m with guanidine hydrochloride is less than the wild-type value and is either constant or increases slightly with increasing denaturant; the value of m with urea is also less than wild type but shows a marked increase with increasing denaturant concentration, often approaching but never exceeding the wild-type value. For class II mutants, m is constant and is greater than wild type in both denaturants, with the increase being consistently larger in guanidine hydrochloride than in urea. When double or triple mutants are constructed from members of the same mutant class, the change in m is usually the sum of the changes produced by each mutation in isolation. One plausible explanation for these altered patterns of denaturation is that chain-chain or chain-solvent interactions in the denatured state have been modified--interactions which appear to involve hydrophobic groups. 相似文献
60.
Spectrally pure reaction center preparations from Chloroflexus aurantiacus have been obtained in a stable form; however, the product contained several contaminating polypeptides. The reaction center pigment molecules (probably three bacteriochlorophyll a and three bacteriopheophytin a molecules) are associated with two polypeptides (Mr = 30000 and 28000) in a reaction center complex of Mr = 52000. No carotenoid is present in the complex. These data together with previous spectral data suggest that the Chloroflexus reaction center represents a more primitive evolutionary form of the purple bacterial reaction center, and that it has little if any relationship to the green bacterial component. A reaction center preparation from Rhodopseudomonas sphaeroides R26 was fully denatured at 50°C while the Chloroflexus reaction center required higher temperatures (70–75°C) for complete denaturation. Thus, an intrinsic membrane protein of a photosynthetic thermophile has been demonstrated to have greater thermal stability than the equivalent component of a mesophile. 相似文献