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991.
The purpose of this study was to use a four-fluid nozzle spray drier as a new one-step method for preparing rifampicin (RFP)-containing
mannitol microparticles. A RFP-acetone/methanol (2:1) solution and aqueous solutions of mannitol (MAN) were simultaneously
supplied through different liquid passages of a four-fluid nozzle spray drier and then dried to obtain MAN microparticles
containing RFP. Using a cascade impactor, the in vitro aerosol performance of RFP powder and RFP-MAN microparticles with 1:5, 1:10, and 1:20 ratios was compared. The in vivo retention of RFP in the lungs of rats after intratracheal administration of 1:20 RFP-MAN microparticles was also compared.
The RFP-MAN microparticles had better aerosol performance than RFP powder and delivery to the lung stages improved as the
fraction of MAN was increased. For the 1:20 RFP-MAN microparticles, deposition in stages 2–7 was approximately 43%, which
is sufficient for treatment. Approximately 8% of the RFP-MAN microparticles were deposited in stages 6–7, which corresponds
to alveoli containing alveolar macrophages. The initial retention of RFP in the lung following pulmonary delivery of 1:20
RFP-MAN microparticles was higher than following oral or intravenous administration of RFP, but the elimination was rapid,
resulting in the disappearance of RFP from the lung within 4 h. The plasma concentration–time profile of RFP after intratracheal
administration of 1:20 RFP-MAN microparticles was consistent with the profile for RFP retention in the lung. Addition of cholesterol
or phosphatidylcholine to RFP had little effect on its retention in the lung. The RFP-MAN microparticles were effective for
delivery of RFP to the lung, but the RFP rapidly removed from the lung into the blood circulation. This study demonstrated
that RFP-containing MAN microparticles prepared in one step using the four-fluid nozzle spray drier efficiently deliver RFP
to the lung, although methods must be developed to prolong its retention and improve targeting to alveolar macrophages. 相似文献
992.
对不同葡萄糖浓度下光滑球拟酵母分批发酵生产丙酮酸的动力学模型分析发现, 葡萄糖浓度是影响光滑球拟酵母发酵生产丙酮酸过程功能的关键因素。在发酵初始阶段, 低浓度葡萄糖可维持较高的菌体比生长速率; 对数生长中前期, 葡萄糖快速进料使菌体浓度接近最大值, 并实现碳流从菌体生长转向丙酮酸积累; 对数生长后期葡萄糖浓度控制在33.4 g/L以维持高丙酮酸对葡萄糖产率系数 (0.71 g/g)。采用奇异控制的葡萄糖流加方式, 在7 L发酵罐上控制不同发酵阶段葡萄糖浓度处于最佳水平以强化光滑球拟酵母过程功能, 丙酮酸产量 (83.1 g/L)、产率 (0.621 g/g)、生产强度[1.00 g/(L·h)]与分批发酵对比, 分别提高了21.3%、21.6%和29.9%。 相似文献
993.
棉花花铃期短期干旱下氮素对干物质及氮素累积分配的影响 总被引:4,自引:0,他引:4
通过盆栽试验进行水分(正常灌水和干旱后复水)和施氮处理(0、240、480kgN·hm-2),研究花铃期短期干旱再复水后氮素对棉花各器官干物质重、氮素累积与分配及产量与品质的影响.结果表明:花铃期土壤干旱显著降低了棉株各器官的干物质重与氮素累积量,而增大了棉株各器官的氮素含量,同时亦降低了棉株干物质与氮素在叶片中的分配指数,但提高了在根系的分配指数,从而增大了根冠比;增施氮肥可以提高干旱条件下棉株的干物质重与氮素累积量,但亦增大水分胁迫指数.复水对干旱处理棉株生长具有明显的补偿效应,尤其是根系的干物质重与氮素累积量显著高于相应正常灌水处理,且增施氮肥可以提高棉株的补偿效应.花铃期干旱结束时与复水后第10天,干旱处理棉株均以240kgN·hm-2水平下的生殖器官干物质重与分配指数最高,而根冠比最小,地上部与地下部生长最为协调,最终籽棉产量最高、纤维品质最优;而施氮不足(0kgN·hm-2)或过量(480kgN·hm-2)均不利于棉花产量的提高与纤维品质的改善. 相似文献
994.
D.G. Valencia M.P. SerranoR. Lázaro M.A. LatorreG.G. Mateos 《Animal Feed Science and Technology》2008
Two trials were conducted to test the effect of micronization (very fine grinding) of soya bean meal (SBM) and fullfat soya bean (FFSB) on productive performance and digestive traits of piglets. The experimental design was completely randomized with four treatments arranged factorially (SBM and FFSB, micronized and ground). The mean particle size (MPS) was 47 and 881 μm for the SBM and 41 and 778 μm for the FFSB, micronized and ground, respectively. In trial 1 growth traits from 23 to 45 days of age and the coefficient of total tract apparent digestibility (CTTAD) of dietary components at 33 and 45 days of age were assessed. In trial 2 the coefficient of ileal apparent digestibility (CIAD) of dietary components, the pH of the gastro intestinal tract (GIT) and the weight of digestive organs and spleen were measured at 45 days of age. From 23 to 33 days of age pigs fed SBM grew faster (253 g/d versus 213 g/day; P<0.05) and were more efficient (0.87 g/g versus 0.98 g/g; P<0.01) than pigs fed FFSB. For the entire experiment (23–45 days of age) pigs fed SBM tended to grow more (360 g/day versus 324 g/day) and to eat more feed (414 g/day versus 380 g/day) than pigs fed FFSB (P<0.10). The CTTAD of crude protein (0.798 g/kg versus 0.778 g/kg), organic matter (0.864 g/kg versus 0.839 g/kg) and gross energy (0.849 g/kg versus 0.830 g/kg) were higher for pigs fed SBM than for pigs fed FFSB (P<0.001). In addition, CIAD of organic matter (0.765 g/kg versus 0.705 g/kg) and gross energy (0.761 g/kg versus 0.711 g/kg) were higher for SBM than for FFSB diets (P<0.001). The pH of the different segments of the GIT was not affected by the protein source (P>0.10). Particle size did not affect any trait studied (P>0.10). The poor performance and digestibility of pigs fed FFSB as compared to pigs fed SBM might be related to the conditions applied during processing. It is concluded that pigs fed soya bean meal perform better than pigs fed FFSB and that micronization of the soya protein sources does not affect any trait studied. 相似文献
995.
Despite it being a component of the seston we know very little about fluvial (waterborne) pollen and spore (palynomorph) transport.
This paper presents the results of a monitoring programme conducted over two years and at a catchment scale in South West
England. A hierarchical monitoring network was established with flood peak samples taken at 9 sub-catchments, intra-hydrograph
samples taken in two sub-catchments and time-integrated sampling undertaken at one location. In addition sampling was undertaken
of probable palynomorph sources such as channel bed and bank sediments, and the airborne pollen flux was monitored using modified
Tauber traps. The results support previous research in illustrating how the vast majority of fluvial pollen and spores are
transported during floods (91%) and that the main control on waterborne palynomorph assemblages is the catchment vegetation
and its spatial distribution but with a long-distance (extra-catchment) component. However, strong seasonal effects are also
shown, and the importance of distinctive sources such as the riparian input, bed re-suspension and overland flow into drains
and tributaries is revealed. Fine sediment in river pools appears to act as a selective store of damaged cereal type pollen
grains derived from arable fields. Although pollen does form part of composite particles the data presented here suggest that
the majority of the pollen is transported as single grains. Fluvial palynomorph loading is strongly dependant upon discharge
and so concentrations in laminated or varved sediments could be regarded as a proxy for flood magnitude.
Handling editor: J. Saros 相似文献
996.
A total of 188 yearling steers of predominantly Angus and Hereford breeds, with mean body weight of 299 kg, were used in this study, which started on 8 April and finished on 3 October, to assess the effects of environmental factors on feed intake of steers in various housing systems. Housing consisted of outside lots with access to overhead shelter, outside lots with no overhead shelter and a cold confinement building. Ad libitum corn, 2.27 kg of 35% dry matter whole plant sorghum silage and 0.68 kg of a 61% protein-vitamin-mineral supplement was offered. Feed that was not consumed was measured to determine feed intake. The temperature data were recorded by hygro-thermographs. Hourly temperatures and humidity were used to develop weather variables. Regression analysis was used and weather variables were regressed on dry matter intake (DMI). When addition of a new variable did not improve R (2) more than one unit, then the number of variables in the model was truncated. Cattle in confinement had lower DMI than those in open lots and those in open lots with access to an overhead shelter (P < 0.05). Cattle in outside lots with access to overhead shelter had similar DMI compared to those in open lots (P = 0.065). Effect of heat was predominantly displayed in August in the three housing systems. In terms of explaining variation in DMI, in outside lots with access to overhead shelter, average and daytime temperatures were important factors, whereas in open lots, nocturnal, peak and average temperatures were important factors. In confinement buildings, the previous day's temperature and humidity index were the most important factors explaining variation in DMI. Results show the effect of housing and weather variables on DMI in summer and when considering these results, cattle producers wishing to improve cattle feedlot performance should consider housing conditions providing less stress or more comfort. 相似文献
997.
998.
Vanadium-dependent peroxidase activity in extracts of Ascophyllum nodosum growing in the intertidal region close to Roscoff/France, and algal vanadium levels, followed approximately similar seasonal variation, as deduced from a study lasting from April 2005 to March 2006. High peroxidase (PO) activity was found in extracts obtained from algae collected in between midwinter to spring [∼100-190 U per g dry mass (dm), triiodide assay] with a maximum in April. Periods of reduced PO activity lasted from summer to early winter (∼50-90 U per g dm). High vanadium levels (1.5-2.2 mg kg−1 dm) were found in algae collected from midwinter to spring, whereas reduced levels (0.6-1.4 mg kg−1 dm) were found in summer to early winter. 相似文献
999.
Nitrogen fertiliser rate and post-anthesis waterlogging effects on carbohydrate and nitrogen dynamics in wheat 总被引:1,自引:0,他引:1
Waterlogging is predicted to increase in both magnitude and frequency along with global warming, and will become one of the
most severe adversities for crop production in many regions. Nitrogen is considered to be an effective up-regulatory nutrient
for crops grown under stress and non-stress conditions. In this study, we try to evaluate N fertiliser effects on contents
of carbohydrate and N dynamics, dry matter accumulation in shoot, yield under post-anthesis waterlogging. Waterlogging after
anthesis significantly reduced grain yield due to decrease in thousand-kernel-weight and in grain number per spike. High N
fertiliser application aggravated grain yield loss due to post-anthesis waterlogging. These yield losses were related to the
decreases in dry matter accumulation, redistribution of stored photosynthate to the grain, and the conversion capacity from
carbohydrate to starch in grain. The decrease in dry matter accumulation could be attributed to the reduced activities of
Pn (photosynthesis) and SPS (sucrose phosphate synthase) in the flag leaf, while the low capacity in starch synthesis could
be explained by the reduced activities of sucrose synthase (SS) and soluble starch synthase (SSS) in grain. Total N uptake
in shoot was also reduced, which could contribute to the losses in biomass and yield by waterlogging. The decrease in Pn was
inconsistent with the increase in N content in the flag leaf at high N fertiliser application under post-anthesis waterlogging. 相似文献
1000.
Changes in nitrogen resorption traits of six temperate grassland species along a multi-level N addition gradient 总被引:1,自引:0,他引:1
Ju-Ying Huang Xiao-Guang Zhu Zhi-You Yuan Shi-Huan Song Xin Li Ling-Hao Li 《Plant and Soil》2008,306(1-2):149-158
Nitrogen (N) resorption from senescing leaves is an important mechanism of N conservation for terrestrial plant species, but
changes in N-resorption traits over wide-range and multi-level N addition gradients have not been well characterized. Here,
a 3-year N addition experiment was conducted to determine the effects of N addition on N resorption of six temperate grassland
species belonging to three different life-forms: Stipa krylovii Roshev. (grass), Cleistogenes squarrosa (T.) Keng (grass), Artemisia frigida Willd. (semishrub), Melissitus ruthenica C.W.Wang (semishrub and N-fixer), Potentilla acaulis L. (forb) and Allium bidentatum Fisch.ex Prokh. (forb). Generally, N concentrations in green leaves increased asymptotically for all species. N concentrations
in senescent leaves for most species (5/6) also increased asymptotically, except that the N concentration in senescent leaves
of A. bidentatum was independent of N addition. N-resorption efficiency decreased with increasing N addition level only for S. krylovii and A. frigida, while no clear responses were found for other species. These results suggest that long-term N fertilization increased N
uptake and decreased N-resorption proficiency, but the effects on N-resorption efficiency were species-specific for different
temperate grassland species in northern China. These inter-specific differences in N resorption may influence the positive
feedback between species dominance and N availability and thus soil N cycling in the grassland ecosystem in this region. 相似文献