Protection of polyribosomes by attachment to membranes and by diethylpyrocarbonate during their extraction from Vicia faba

It was shown that membrane-bound polyribosomes of developing cotyledons wore protected during extraction by their membrane attachment and by the presen     

Isolation of hamster brain polyribosomes-cytoskeleton complexes

We have developed a method for the isolation of a brain subcellular fraction enriched in both highly aggregated polyribosomes and cytoskeletal proteins. This method is based on gentle homogenization of brain tissue and low speed centrifugation. The mechanism of association of polyribosomes to cytoskeletal structures has been studied by in vitro treatment of this fraction with polyribosome-disaggregating agents. RNase and EDTA, while succeeding in completely disrupting them into monosomes or subunits, did not release them from cytoskeleton. Puromycin showed no noticeable effect.Abbreviations CSK cytoskeleton - DOC sodium deoxycholate - PR polyribosome - PRCSC polyribosomes-cytoskeleton complex     

Cinetique d'action de l'acide 2-chloro ethylphosphonique sur les polyribosomes du latex d'Hevea brasiliensis

Effects of 2-chloroethylphosphonic acid on the latex of Hevea brasiliensis were studied during 7 days after its application on the tapping panel of the tree. Ribosome polymerisation shows a dramatic rise within 12 hr after treatment. This shift is overcome at first without rRNA synthesis. Increase of latex production and latex pH exhibit similar features and start 24 hr after application of the, stimulant. After 4 days, rRNA concentration and the stability of the lutoïds (microvacuoles with lysosomal characteristics) change significantly.