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61.
A. Falconi D. Lorenzo S. Curti F. R. Morales M. Borde 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》1997,181(2):143-151
The present study was designed to examine the synaptic events in neurons of the pacemaker nucleus of Gymnotus carapo during the increase in rate of the electric organ discharge following activation of Mauthner cells. Pacemaker and relay cells
were investigated using intracellular recordings which were performed under two different conditions: (1) with the pacemaker
nucleus spontaneously discharging and (2) after its activity was abolished by anesthesia. Mauthner axon activation induced
an increase in the rate of pacemaker cell discharges. This response was accompanied by an increase in the slope of the pacemaker
potential (up to 110%) and a depolarization of these cells. The discharges of relay cells followed one to one those of pacemaker
cells. In contrast to that observed in pacemaker cells, only brief depolarizing antidromic effects could be evoked in relay
cells after Mauthner axon activation. In quiescent pacemaker cells, Mauthner cell activation induced a prolonged (up to 500 ms)
depolarizing potential with an average amplitude of 1.92 ± 0.82 mV; its latency was 4.43 ± 1.14 ms. Our data indicate that,
within the pacemaker nucleus, the population of pacemaker cells is the only target for Mauthner cell-evoked, short-latency
excitatory synaptic actions.
Accepted: 1 March 1997 相似文献
62.
WILLIAM H. CLEMENTS MARJORIE L. BROOKS† DONNA R. KASHIAN‡ ROBERT E. ZUELLIG§ 《Global Change Biology》2008,14(9):2201-2214
Changes in regional climate in the Rocky Mountains over the next 100 years are expected to have significant effects on biogeochemical cycles and hydrological processes. In particular, decreased discharge and lower stream depth during summer when ultraviolet radiation (UVR) is the highest combined with greater photo-oxidation of dissolved organic materials (DOM) will significantly increase exposure of benthic communities to UVR. Communities in many Rocky Mountain streams are simultaneously exposed to elevated metals from abandoned mines, the toxicity and bioavailability of which are also determined by DOM. We integrated field surveys of 19 streams (21 sites) along a gradient of metal contamination with microcosm and field experiments conducted in Colorado, USA, and New Zealand to investigate the influence of DOM on bioavailability of heavy metals and exposure of benthic communities to UVR. Spatial and seasonal variation in DOM were closely related to stream discharge and significantly influenced heavy metal uptake in benthic organisms. Qualitative and quantitative changes in DOM resulting from exposure to sunlight increased UV-B (290–320 nm) penetration and toxicity of heavy metals. Results of microcosm experiments showed that benthic communities from a metal-polluted stream were tolerant of metals, but were more sensitive to UV-B than communities from a reference stream. We speculate that the greater sensitivity of these communities to UV-B resulted from costs associated with metal tolerance. Exclusion of UVR from 12 separate Colorado streams and from outdoor stream microcosms in New Zealand increased the abundance of benthic organisms (mayflies, stoneflies, and caddisflies) by 18% and 54%, respectively. Our findings demonstrate the importance of considering changes in regional climate and UV-B exposure when assessing the effects of local anthropogenic stressors. 相似文献
63.
1. The abundance, production and control of pelagic heterotrophic nanoflagellates (HNF) in the Lower River Rhine (Germany) were investigated. Field samples (live‐counting technique) were taken at least every 2 weeks at Cologne (km 685) over a period of 20 months. In addition, Lagrangian sampling was carried out 160 km downstream of Cologne (Kalkar–Grieth, km 845) over a period of 12 months. Potential HNF growth rates and loss rates caused by planktonic predators were estimated in the laboratory (size fractionation experiments) and compared with the changes in HNF‐density in a water parcel flowing downstream. 2. Mean abundance (±SD) ranged from 7 ± 6 to 4890 ± 560 individuals mL–1 and was positively correlated with discharge. Heterotrophic nanoflagellates abundance increased up to 30‐fold during flood events, whereas there were only minor changes in the taxonomic composition. 3. HNF growth rate ranged from 0.16 ± 0.12 to 1.98 ± 0.10 day–1. Between 0 and 95% (mean: 32%) of the gross production was lost to planktonic predators; a larger portion between 0 and 195% (mean: 64%) of the HNF gross production was lost by other means. 4. There may be an important role for benthic predators in the control of pelagic HNF. First, production of HNF was high and grazing losses to planktonic predators low at times when HNF abundance was low. Secondly, high in situ loss rates (not explicable by planktonic predators), which were positively related to temperature, indicate the importance of biotic interactions. Thirdly, the dependence of HNF abundance on discharge indicated a decrease grazing intensity with rising water levels (increase in water volume/colonised river bed ratio). 5. The impact of discharge on planktonic HNF mediated by the grazing impact of benthic predators was modelled, showing a good fit with the field data. 相似文献
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Gonadectomized male (n = 5) and female (n = 5) and intact intersex goats (n = 2) were injected i.m. with 50 mug 17(beta)-estradiol benzoate (EB). After treatment, there was a transient 6- to 9-hr decrease in circulating levels of LH followed by a preovulatory-like discharge of LH in all goats. Release peaked at 12 to 18 hr after EB treatment. The magnitude of discharge and the time from treatment until peak of release were not influenced by the goat's sex. These findings suggested that the positive feedback effects of estrogen on LH release were not sexually differentiated in the goat. Since tonic concentrations of LH prior to EB treatment were not different among the groups, the studies also suggested that the intersex goats lacked the inhibitory gonadal influences on gonadotropin release that characterize intact animals. 相似文献
66.
67.
1. Local assemblage structure, from a deterministic perspective, is presumably dictated by the regional species pool as well as regulated by local factors. We examined the relationships of the regional species pool and local hydrological characteristics to local species richness of North American freshwater fishes using data sets collected during the National Water Quality Assessment program conducted by the United States Geological Survey. 2. We predicted that local species richness is ultimately constrained by the composition of the regional species pool and further associated with local hydrological factors. Moreover, we predicted that variation in local species richness within major families can be explained by different combinations of hydrological characteristics that represent lineage‐specific responses to the environment. 3. Daily discharge and regional and local species richness data were assembled from 41 stream localities across the United States. Multiple stepwise regressions were conducted to predict local species richness, based on regional species richness, mean discharge and hydrological characteristics quantified by nine variables characterising flow variability. Species richness at each site was calculated for the entire assemblage as well as within the four most species‐rich families in the data set (Catostomidae, Centrarchidae, Cyprinidae and Percidae). 4. Local species richness was best predicted by a combination of regional species richness and discharge magnitude when all species were considered. Regional species richness was a significant explanatory variable of local species richness for three of four families (Catostomidae, Centrarchidae, Cyprinidae), but not for Percidae. Local richness in Centrarchidae and Cyprinidae was positively correlated with temporal flow variability as well as high and low flow duration, respectively, while richness in Catostomidae and Percidae tended to be associated with discharge volume. In addition, local species richness for three of the four major families was positively correlated with species richness of the other families in the assemblage, potentially suggesting the influence of local habitat quality and heterogeneity. 5. Results suggest the importance of the combined influences of the regional species pool and local hydrological characteristics on local richness in freshwater fishes, with variation in richness within each family predicted by different characteristics of flow regimes. 相似文献
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70.
Xiaoming Yue 《Molecular simulation》2017,43(12):935-944
Due to the unique and superior properties of using thermal energy to machine electrically conductive material regardless of its hardness, electrical discharge machining has been widely used and has become indispensable in the manufacturing industry. However, the incompleteness and imperfection of the fundamental theory behind it seriously hinders its further application and development. In this work, a single pulse discharge in deionised water was simulated to study the material removal motivity and mechanisms with molecular dynamics simulations. The results show that during the discharge process, there exists a decreasing pressure gradient along the depth direction towards the surface of the melting area. When the pressure gradient overcomes the atomic bonding forces, the melting material can be ablated from the electrode. Thus, it is not the case that the melting material cannot be removed during the discharge process; material removal occurs throughout the whole discharge process. In addition, it was found that the bulge is formed for two reasons: the main reason is the shearing flow of the melting material caused by the pressure gradient along the radial direction in the melting area, and the other is the accumulation of the spattering material from the opposite electrode. 相似文献