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991.
Fabiana C. P. Tiago Flaviano S. Martins Carlos A. Rosa Regina M. D. Nardi Denise C. Cara Jacques R. Nicoli 《World journal of microbiology & biotechnology》2009,25(4):657-666
Probiotics are defined as live microorganisms, which when administered in adequate amount confer a health benefit to the host.
Most of studied or commercialized probiotics contain bacteria and very few of them present yeast in its composition. In this
last case, the microorganisms almost always belong to Saccharomyces genus. In the present study, it was of interest to screen among 103 non-Saccharomyces yeasts a candidate for probiotic by using in vitro and in vivo criteria. In vitro assays included growth at 37°C and production
of antagonistic compounds against enteropathogenic indicators, and the in vivo assays evaluated the colonization ability of
mouse gastrointestinal tract without pathologic consequences and the protective ability in mice experimentally challenged
with Clostridium difficile. In conclusion, Pichia kluyveri strain 898 showed to be a potential candidate for probiotic use, based on the criteria cited above, particularly as demonstrated
by its protective effect against experimental infection in mice. Interestingly, an in vivo inhibition against C. difficile observed in the animal models did not correlate with the results obtained with the in vitro assays. 相似文献
992.
993.
Claudine Charpentier Anne Colin Anne Alais Jean-Luc Legras 《Antonie van Leeuwenhoek》2009,95(3):263-273
Fifty-four Saccharomyces cerevisiae strains were isolated from Jura “Vin Jaune” velum and characterized by conventional physiological and molecular tests including
ITS RFLP and sequence analysis, karyotyping and inter delta typing. ITS RFLP and sequence revealed a specific group of related
strains different from the specific profile of Sherry flor yeast caused by a 24 bp deletion in the ITS1 region described by
Esteve-Zarzoso et al. (Antonie Van Leeuwenhoek 85:151–158, 2004). Interdelta typing, the most discriminative method, revealed a high diversity of Jura flor yeast strains and gathered strains
in clusters unequally shared between the northern and southern part of the Jura vineyard. The assessment of phenotypic diversity
among the isolated strains was investigated for three wine metabolites (ethanal, acetic acid, and sotolon) from micro scale
velum tests. Except at an early stage of ageing, the production of these metabolites was not correlated to the five genetic
groups obtained by interdelta typing, but correlated to the cellar where strains had been isolated. The different strains
isolated in a cellar produced mostly one type of velum (thin or thick, grey or white); but thin and grey velums, recognized
as responsible for high quality wines, were obtained more frequently for one of the five groups of delta genotypes. 相似文献
994.
995.
Discola KF de Oliveira MA Rosa Cussiol JR Monteiro G Bárcena JA Porras P Padilla CA Guimarães BG Netto LE 《Journal of molecular biology》2009,385(3):889-586
Glutaredoxins (Grxs) are small (9-12 kDa) heat-stable proteins that are ubiquitously distributed. In Saccharomyces cerevisiae, seven Grx enzymes have been identified. Two of them (yGrx1 and yGrx2) are dithiolic, possessing a conserved Cys-Pro-Tyr-Cys motif. Here, we show that yGrx2 has a specific activity 15 times higher than that of yGrx1, although these two oxidoreductases share 64% identity and 85% similarity with respect to their amino acid sequences. Further characterization of the enzymatic activities through two-substrate kinetics analysis revealed that yGrx2 possesses a lower KM for glutathione and a higher turnover than yGrx1. To better comprehend these biochemical differences, the pKa of the N-terminal active-site cysteines (Cys27) of these two proteins and of the yGrx2-C30S mutant were determined. Since the pKa values of the yGrx1 and yGrx2 Cys27 residues are very similar, these parameters cannot account for the difference observed between their specific activities. Therefore, crystal structures of yGrx2 in the oxidized form and with a glutathionyl mixed disulfide were determined at resolutions of 2.05 and 1.91 Å, respectively. Comparisons of yGrx2 structures with the recently determined structures of yGrx1 provided insights into their remarkable functional divergence. We hypothesize that the substitutions of Ser23 and Gln52 in yGrx1 by Ala23 and Glu52 in yGrx2 modify the capability of the active-site C-terminal cysteine to attack the mixed disulfide between the N-terminal active-site cysteine and the glutathione molecule. Mutagenesis studies supported this hypothesis. The observed structural and functional differences between yGrx1 and yGrx2 may reflect variations in substrate specificity. 相似文献
996.
目的探讨pH值和氧气对白念珠菌菌丝形成的影响。方法通过调节Muller—Hinton液体培养基的pH值和去除培养基中的氧气来观察白念珠菌的生长曲线、倍增时间和菌丝形成率的变化。结果在无氧气的液体培养基中,白念珠菌生长缓慢,不能产生菌丝结构,只有酵母细胞形成。生长曲线的延缓期内各组没有明显差异,而在生长的对数期pH3和pH4的条件下念珠菌生长速度明显慢于pH5、pH6、pH7、pH8和pH9。菌丝形成率在pH3、pH4和pH5条件下〈20%,而在pH6、pH7、pH8和pH9条件下可高达70%。结论厌氧条件抑制白念珠菌的菌丝形成,只形成酵母细胞。白念珠菌在pH3—9的范围内均能生长,偏酸性环境有利于白念珠菌酵母形成,偏碱性的环境有助于菌丝的形成。 相似文献
997.
It is well known that the expression noise is lessened by natural selection for genes that are important for cell growth or are sensitive to dosage. In theory, expression noise can also be elevated by natural selection when noisy gene expression is advantageous. Here we analyze yeast genome‐wide gene expression noise data and show that plasma‐membrane transporters show significantly elevated expression noise after controlling all confounding factors. We propose a model that explains why and under what conditions elevated expression noise may be beneficial and subject to positive selection. Our model predicts and the simulation confirms that, under certain conditions, expression noise also increases the evolvability of gene expression by promoting the fixation of favorable expression level‐altering mutations. Indeed, yeast genes with higher noise show greater between‐strain and between‐species divergences in expression, even when all confounding factors are excluded. Together, our theoretical model and empirical results suggest that, for yeast genes such as plasma‐membrane transporters, elevated expression noise is advantageous, is subject to positive selection, and is a facilitator of adaptive gene expression evolution. 相似文献
998.
运用紫外诱变方法成功获得了自絮凝酵母的营养缺陷型突变体,并且优化了诱变方法,证明了通过紫外诱变也可获得自絮凝酵母的营养缺陷型突变体。实验证明,较低的致死率更容易获得突变体,利用制霉菌素的富集可明显减少非缺陷型背景。本实验获得了组氨酸和尿嘧啶营养缺陷型各一株,其中组氨酸缺陷型茵株失去絮凝特性,而尿嘧啶缺陷型保持了良好的絮凝特性。继代实验表明,二株突变体均可以稳定遗传。并利用交配型PCR方法证明了絮凝酵母及其两株突变体与其酿酒酵母亲本类似,均为交配型杂合体。 相似文献
999.
蛋白质相互作用既是蛋白质执行功能的主要方式,也是细胞功能调控网络的结构基础。蛋白质间异常的相互作用及其连锁网络的紊乱是引起许多病理改变的原因。作为功能基因组和蛋白质组研究的重要内容,规模化蛋白质相互作用研究已成为近年国际上研究的热点之一。文章综述了当前规模化蛋白质相互作用研究中的常用技术和常用蛋白质相互作用数据库,研究者可根据研究需要和技术特点利用这些资源。 相似文献
1000.
Since the estrogen receptor α (ERα) and β (ERβ) are thought to mediate different biological effects, there is intense interest
in designing or screening subtype-selective ER ligands. Here, we constructed a biosensor identified as bipartite recombinant
yeast system (BRYS) to screen and evaluate subtype-selective ER ligands. Uniform design and immunoblotting was used to determine
an optimal dose of copper which control the expression of ERs through a copper inducible metallothionine promoter (CUP1).
Some chemicals including natural estrogen (17β-estradiol), specific estrogen receptor agonist (PPT and DPN), and phytoestrogens
(genistein) were tested to validate this system. There was obvious and anticipative discrimination between the agonistic activities
when these chemicals were identified and characterized. Furthermore, antagonist (ICI 182,780), which could antagonize the
transactivation of estrogen, and chromatin immunoprecipitation (ChIP) were used to confirm that the agonistic effects were
mediated through ERs. Comparative studies showed that this system was reproducible and sensitive. 4.7 pM (1.3 ng/l) estrogen
was the lowest concentration that could be detected to ERα and 0.12 nM (33.5 ng/l) for ERβ. The results from our study showed
that in vitro screening for subtype-selective ER ligands could be conducted in a simple yeast system that is rapid, sensitive,
reliable, and quantitative.
An erratum to this article can be found at 相似文献