全文获取类型
收费全文 | 354篇 |
免费 | 49篇 |
国内免费 | 246篇 |
出版年
2024年 | 2篇 |
2023年 | 5篇 |
2022年 | 22篇 |
2021年 | 15篇 |
2020年 | 29篇 |
2019年 | 34篇 |
2018年 | 22篇 |
2017年 | 21篇 |
2016年 | 26篇 |
2015年 | 20篇 |
2014年 | 22篇 |
2013年 | 34篇 |
2012年 | 21篇 |
2011年 | 28篇 |
2010年 | 26篇 |
2009年 | 29篇 |
2008年 | 27篇 |
2007年 | 44篇 |
2006年 | 25篇 |
2005年 | 30篇 |
2004年 | 21篇 |
2003年 | 23篇 |
2002年 | 20篇 |
2001年 | 18篇 |
2000年 | 16篇 |
1999年 | 9篇 |
1998年 | 6篇 |
1997年 | 6篇 |
1996年 | 8篇 |
1995年 | 4篇 |
1994年 | 5篇 |
1993年 | 2篇 |
1992年 | 4篇 |
1991年 | 6篇 |
1990年 | 1篇 |
1989年 | 5篇 |
1988年 | 2篇 |
1987年 | 2篇 |
1986年 | 2篇 |
1985年 | 1篇 |
1984年 | 1篇 |
1982年 | 2篇 |
1980年 | 1篇 |
1977年 | 1篇 |
1974年 | 1篇 |
排序方式: 共有649条查询结果,搜索用时 15 毫秒
641.
Coastal wetland stability and structure can be significantly affected by littoral processes when they are close to the shoreline.
Thus, under certain conditions, the combination of shoreline dynamics and direct wave action during storms can influence the
stability of marshes and vegetation community composition. This interaction between littoral dynamics and coastal wetlands
is illustrated by analysing processes taking place in the Buda Island (Ebro delta, NW Spain), where a coastal lagoon very
close to a retreating shoreline exists. Two main time scales have been found to be relevant for interaction of coastal processes
with ecosystem dynamics, the decadal and episodic scales. The decadal scale determines the average trend in beach width and
directly controls the potential loss of wetland surface. The episodic scale is linked to the occurrence of wave and storm
surge events and it determines a pulsing stress in the ecosystem through flooding, being key parameters to determine their
direct influence the intensity and repetition of these events. 相似文献
642.
A J Teale J Wambugu P S Gwakisa G Stranzinger D Bradley S J Kemp 《Animal genetics》1995,26(4):243-248
A small number of west African Bos taurus cattle breeds, including the N'Dama, constitute a valuable genetic resource by virtue of their ability to remain productive under trypanosomiasis challenge. However, introgression of Bos indicus genes into the trypanotolerant breeds, particularly by introduction of zebu bulls, is a threat to this resource. This work describes the characterization and cloning of a bovine randomly amplified polymorphic DNA (RAPD) that is generated in polymerase chain reaction (PCR) with the 10 base primer ILO1065 from Bos indicus male templates, but not from B. taurus male templates or female templates of either type. Male-specific sequences with homology to the RAPD also occur in B. taurus breeds. This suggests that the polymorphism may be due to base substitution(s) in an ILO1065 priming site, or insertion/deletion events either affecting priming sites or occurring between sites on the cattle Y chromosome. We have shown that cattle, whether of B. indicus or B. taurus phenotype, which possess a typically B. indicus metaphase Y chromosome on the basis of QFQ banding, have a B. indicus ILO1065-generated genotype. The ILO1065-primed RAPD can be used in a simple dot blot assay as a probe of RAPD-PCR products, to provide a convenient, reliable and effective means of detecting introgression of zebu genes in B. taurus cattle populations. 相似文献
643.
In this study dendrochronological methods are used to investigate tree growth of several coniferous species growing on serpentine outcrops in the subalpine region. Though stands are thriving on the same parent material, almost barren sites covered with Scots pine trees (Pinus sylvestris/ L.) contrast sharply with adjacent dense stands composed of mixed coniferous species. To find a reasonable explanation for this vegetation pattern and to evaluate the influence of serpentine soil on radial tree growth annual variability in ring-widths was analyzed and compared between both types of stands. Tree-ring analyses show that sparse tree cover on steep slopes might be caused by environmental stress factors especially unfavourable physical soil properties such as instability and dryness. Several abrupt growth reductions followed by slow release in ring-widths indicate repeated mechanical injury to the root system due to soil erosion. On the other hand the aggravating impact of anthropogenic disturbances (tree felling) on this ecologically fragile serpentine area could be determined and dated. There was no evidence that radial tree growth of coniferous species at this site could be limited by toxic heavy metals in the soil. 相似文献
644.
In January 1989 a major breach in an embanked section of the Grand Canal occurred. As a result, a 2.5-km long section required complete reconstruction. This work was completed in approximatley 12 months, at a cost of IR1.5 million. The canal was rewatered in March 1990. The banksides, above normal water level, were dressed with a layer of moss peat and seeded with a mixture of grasses. The grass roots failed to bind the peat to the sub-layer of Puddle Clay and significant erosion resulted in the season of treatment. In order to halt the harmful erosion and to expedite the natural reed colonisation process, roots and rhizomes from established monocotyledonous plant colonies, external to the canal, were acquired. This paper presents the findings from transplantation trials using Schoenoplectus lacustris, Glyceria maxima and Phragmites australis from river and lake habitats and comments on the efficiency and cost-effectiveness of this operation. The value of reeds in amenity watercourses is discussed. 相似文献
645.
During meiosis, the recombination-initiating DNA double-strand breaks (DSBs) are repaired by crossovers or noncrossovers (gene conversions). While crossovers are easily detectable, noncrossover identification is hampered by the small size of their converted tracts and the necessity of sequence polymorphism. We report identification and characterization of a mouse chromosome-wide set of noncrossovers by next-generation sequencing of 10 mouse intersubspecific chromosome substitution strains. Based on 94 identified noncrossovers, we determined the mean length of a conversion tract to be 32 bp. The spatial chromosome-wide distribution of noncrossovers and crossovers significantly differed, although both sets overlapped the known hotspots of PRDM9-directed histone methylation and DNA DSBs, thus supporting their origin in the standard DSB repair pathway. A significant deficit of noncrossovers descending from asymmetric DSBs proved their proposed adverse effect on meiotic recombination and pointed to sister chromatids as an alternative template for their repair. The finding has implications for the molecular mechanism of hybrid sterility in mice from crosses between closely related Mus musculus musculus and Mus musculus domesticus subspecies. 相似文献
646.
Wind has been proposed as a driving factor in determining vegetation patterns, but there are few dynamic models that include both vegetation and wind. In this paper, we present a dynamic model to investigate how a vegetation pattern is generated and affected by wind. In the model, the effects of prevailing wind and non-prevailing wind on sand and vegetation are modeled respectively as advection terms and diffusion terms. With these considerations and proper parameter values that satisfy Turing bifurcation conditions, labyrinth and banded vegetation patterns are obtained in two situations of wind. By changing wind transportation capacity, we simulate the adaptation process from one vegetation pattern to another. With environmental changes of large amplitude, the width of vegetation bands varies while the wavelength can increase but does not decrease in our simulation. Then we describe the difference between simulated patterns and real patterns. And in the discussion, we explain the mechanism that forming patterns and the consistency of this research with other studies. 相似文献
647.
Soil erosion in reclaimed mines may affect plant colonization and performance, and may compromise restoration success; however, the magnitude of this effect has seldom been quantified. We monitored the dynamics of vegetation (seed bank density, seedling emergence, plant mortality, and seed production) during a growing season (2003–2004) in three constructed slopes with differing past erosion rates. The slopes are located in the Utrillas coalfield in Spain, which experiences a Mediterranean‐continental climate. In the most eroded slope, soil water availability was lower—especially in the interrill areas—and seedling emergence rate, plant survival, and seed production were also significantly lower than on the less eroded slopes. We found that vegetation recovery is dramatically constrained when rill erosion rate is 17 t ha?1 yr?1 and plant cover is 30%, but this effect disappears when plant cover is higher than 60%. Soil erosion in constructed slopes appears to inhibit natural plant colonization processes by increasing runoff water loss over the long‐term. Thus, when rill erosion networks develop, human intervention would be needed to minimize the loss of water and facilitate vegetation colonization. 相似文献
648.
Jocelyne Favet Ales Lapanje Adriana Giongo Suzanne Kennedy Yin-Yin Aung Arlette Cattaneo Austin G Davis-Richardson Christopher T Brown Renate Kort Hans-Jürgen Brumsack Bernhard Schnetger Adrian Chappell Jaap Kroijenga Andreas Beck Karin Schwibbert Ahmed H Mohamed Timothy Kirchner Patricia Dorr de Quadros Eric W Triplett William J Broughton Anna A Gorbushina 《The ISME journal》2013,7(4):850-867
Ancient mariners knew that dust whipped up from deserts by strong winds travelled long distances, including over oceans. Satellite remote sensing revealed major dust sources across the Sahara. Indeed, the Bodélé Depression in the Republic of Chad has been called the dustiest place on earth. We analysed desert sand from various locations in Chad and dust that had blown to the Cape Verde Islands. High throughput sequencing techniques combined with classical microbiological methods showed that the samples contained a large variety of microbes well adapted to the harsh desert conditions. The most abundant bacterial groupings in four different phyla included: (a) Firmicutes—Bacillaceae, (b) Actinobacteria—Geodermatophilaceae, Nocardiodaceae and Solirubrobacteraceae, (c) Proteobacteria—Oxalobacteraceae, Rhizobiales and Sphingomonadaceae, and (d) Bacteroidetes—Cytophagaceae. Ascomycota was the overwhelmingly dominant fungal group followed by Basidiomycota and traces of Chytridiomycota, Microsporidia and Glomeromycota. Two freshwater algae (Trebouxiophyceae) were isolated. Most predominant taxa are widely distributed land inhabitants that are common in soil and on the surfaces of plants. Examples include Bradyrhizobium spp. that nodulate and fix nitrogen in Acacia species, the predominant trees of the Sahara as well as Herbaspirillum (Oxalobacteraceae), a group of chemoorganotrophic free-living soil inhabitants that fix nitrogen in association with Gramineae roots. Few pathogenic strains were found, suggesting that African dust is not a large threat to public health. 相似文献
649.
Gliricidia sepium provides a variety of products important for rural communities in tropical countries. Native populations in Meso-America currently form an important source of seed for distribution to farmers, but concerns centre on mechanisms which may lead to their genetic erosion, including anthropogenic dispersal and subsequent introgression from the related species, G. maculata. Populations of Gliricidia were examined genetically using approaches based on the polymerase chain reaction to test for interspecific hybridization and introgression between G. sepium and G. maculata. Analysis involved 13 RAPD and two RFLP-PCR markers which were identified to have species-diagnostic distributions. Data from both approaches corresponded and indicated three locations where multilocus genotypes were consistent with an hybrid origin. Data at one of these sites was consistent with introgression following hybridization. The hybrid origin of populations was supported by the intermediate geographical location of these sites to ‘pure’ populations of each species. Analysis of maternally inherited organellar DNA, which involved the detection of SSCPs in mitochondrial DNA amplification products, allowed further delineation of genetic structure among Gliricidia populations. Mitochondrial data indicated a high degree of organelle differentiation between sampled locations and identified G. sepium- and G. maculata-diagnostic haplotypes. This data supported the interpretation of genetic structure based on RAPDs and RFLP-PCR. In addition, cytonuclear analysis allowed the directionality of gene transfer during the formation of hybrid populations to be described. Despite evidence for the occurrence of interspecific hybridization and introgression in Gliricidia, important resource populations of G. sepium on the Pacific coast appear to have retained their genetic integrity. Implications in terms of the conservation and utilization of genetic resources within the genus are discussed. 相似文献