全文获取类型
收费全文 | 1205篇 |
免费 | 151篇 |
国内免费 | 28篇 |
专业分类
1384篇 |
出版年
2024年 | 1篇 |
2023年 | 28篇 |
2022年 | 29篇 |
2021年 | 47篇 |
2020年 | 60篇 |
2019年 | 76篇 |
2018年 | 56篇 |
2017年 | 72篇 |
2016年 | 74篇 |
2015年 | 71篇 |
2014年 | 75篇 |
2013年 | 120篇 |
2012年 | 66篇 |
2011年 | 46篇 |
2010年 | 40篇 |
2009年 | 50篇 |
2008年 | 46篇 |
2007年 | 49篇 |
2006年 | 41篇 |
2005年 | 32篇 |
2004年 | 32篇 |
2003年 | 29篇 |
2002年 | 28篇 |
2001年 | 21篇 |
2000年 | 15篇 |
1999年 | 13篇 |
1998年 | 22篇 |
1997年 | 24篇 |
1996年 | 11篇 |
1995年 | 10篇 |
1994年 | 16篇 |
1993年 | 13篇 |
1992年 | 10篇 |
1991年 | 8篇 |
1990年 | 8篇 |
1989年 | 3篇 |
1988年 | 6篇 |
1987年 | 4篇 |
1986年 | 9篇 |
1985年 | 6篇 |
1984年 | 3篇 |
1983年 | 1篇 |
1982年 | 1篇 |
1981年 | 1篇 |
1980年 | 6篇 |
1979年 | 2篇 |
1978年 | 2篇 |
1976年 | 1篇 |
排序方式: 共有1384条查询结果,搜索用时 15 毫秒
971.
《Saudi Journal of Biological Sciences》2019,26(7):1724-1730
Vitamin D deficiency is a pandemic problem. Non-animal source of vitamin D is obtained from edible mushrooms. Oyster mushroom (Pleurotus ostreatus) was sliced into the size of 1 cm3, 4 cm3 and 9 cm3, and treated with the sun as a natural resource of UVB under subtropical settings in Ethiopia. The content of vitamin D was measured by using high-performance liquid chromatography (HPLC). After sun treatment, there was a significant increment in the content of vitamin D2 from nil to 67.4 ± 28.0 µg/g dry weight (DW). Based on the results of the overall pairwise comparisons, 1 cm3 size of slice group had the highest content of vitamin D2. Duration of sun exposure, sizes of mushroom slices and moisture content were identified as determining factors for vitamin D2 synthesis. Exposing slices of oyster mushroom to the sunlight for <30 min provides the amount that satisfies the current recommended dietary allowance (RDA) of vitamin D without any visible change in color and texture. Thus, sun treatment of oyster mushroom is an effective and economically cheap strategy in the fight against vitamin D deficiency. 相似文献
972.
973.
974.
975.
Feeding habits of the speckled guitarfish Rhinobatos glaucostigma (Elasmobranchii: Rhinobatidae) 下载免费PDF全文
R. E. Lara‐Mendoza J. F. Márquez‐Farías J. C. Román‐Reyes 《Journal of fish biology》2015,87(2):311-322
The stomachs of 464 speckled guitarfish Rhinobatos glaucostigma were sampled from the south‐eastern Gulf of California (GC) to determine diet composition. Numerical indices and prey‐specific index of relative importance (%IPSIR) were used to determine the feeding strategy of the species. An analysis of similarity (ANOSIM) was used to determine differences in diet with respect to sex, season (dry or rainy) and maturity stages (immature or mature). The diversity and niche breadth (by sex, season and maturity) and a general trophic level were determined. The overall diet was dominated by shrimps (%IPSIR = 43·47), amphipods (%IPSIR = 18·89) and crabs (%IPSIR = 18·07). ANOSIM demonstrated differences in the diet by maturity and season, but not by sex. Rainy and dry season diets were dominated by shrimps and amphipods, respectively. Immature specimens fed mainly on amphipods, whereas mature fish preferred shrimps and crabs. Rhinobatos glaucostigma showed a narrow niche breadth with an intermediary trophic level (TL = 3·72) and can be considered as a secondary consumer in the soft‐bottom demersal community of the south‐east GC. Understanding the feeding habits and trophic level of R. glaucostigma is vital to help identify the segments of the population vulnerable to overfishing by artisanal and industrial fisheries, and to aid in conservation and management of this elasmobranch. 相似文献
976.
Teresa Padro Gemma Vilahur Joan Sánchez-Hernández Marta Hernández Rosa M. Antonijoan Antonio Perez Lina Badimon 《Journal of lipid research》2015,56(5):1043-1056
The benefits of dietary phytosterols (PhySs) and long-chain n-3 PUFA (ω3) have been linked to their effects as cholesterol- and triglyceride (TGL)-lowering agents. However, it remains unknown whether these compounds have further metabolic effects on LDL lipid composition. Here, we studied the effects of PhyS- or ω3-supplemented low-fat milk (milk) on the LDL-lipidome. Overweight and moderately hypercholesterolemic subjects (n = 32) were enrolled in a two-arm longitudinal crossover study. Milk (250 ml/day), enriched with either 1.57 g PhyS or 375 mg ω3 (EPA + DHA), was given to the participants during two sequential 28 day intervention periods. Compared with baseline, PhyS-milk induced a higher reduction in the LDL cholesterol (LDLc) level than ω3-milk. LDL resistance to oxidation was significantly increased after intervention with PhyS-milk. Changes in TGL and VLDL cholesterol were only evident after ω3-milk intake. Lipidomic analysis revealed a differential effect of the PhyS- and ω3-milk interventions on the LDL lipid metabolite pattern. Content in LDL-glycerophospholipids was reduced after PhyS-milk intake, with major changes in phosphatidylcholine (PC) and phosphatidylserine subclasses, whereas ω3-milk induced significant changes in the long-chain polyunsaturated cholesteryl esters and in the ratio PC36:5/lysoPC16:0, associated to a reduced inflammatory activity. In conclusion, daily intake of milk products containing PhyS or ω3 supplements induce changes in the LDL-lipidome that indicate reduced inflammatory and atherogenic effects, beyond their LDLc- and TGL-lowering effects. 相似文献
977.
978.
Dietary restriction (DR) extends lifespan in an impressively wide array of species spanning three eukaryotic kingdoms. In sharp contrast, relatively little is known about the effects of DR on functional senescence, with most of the work having been done on mice and rats. Here we used Drosophila melanogaster to test the assumption that lifespan extension through DR slows down age-related functional deterioration. Adult virgin females were kept on one of three diets, with sucrose and yeast concentrations ranging from 7% to 11% to 16% (w/v). Besides age-specific survival and fecundity, we measured starvation resistance, oxidative stress resistance, immunity, and cold-stress resilience at ages 1, 3, 5, and 7 weeks. We confirmed that DR extends lifespan: median lifespans ranged from 38 days (16% diet) to 46 days (11% diet) to 54 days (7% diet). We also confirmed that DR reduces fecundity, although the shortest-lived flies only had the highest fecundity when males were infrequently available. The most striking result was that DR initially increased starvation resistance, but strongly decreased starvation resistance later in life. Generally, the effects of DR varied across traits and were age dependent. We conclude that DR does not universally slow down functional deterioration in Drosophila. The effects of DR on physiological function might not be as evolutionarily conserved as its effect on lifespan. Given the age-specific effects of DR on functional state, imposing DR late in life might not provide the same functional benefits as when applied at early ages. 相似文献
979.
Lara del Campo Amanda Snchez‐Lpez Mercedes Salaices Ryan A. von Kleeck Elba Expsito Cristina Gonzlez‐Gmez Lorena Cuss Gabriela Guzmn‐Martínez Jesús Ruiz‐Cabello Manuel Desco Richard K. Assoian Ana M. Briones Vicente Andrs 《Aging cell》2019,18(3)
Vascular stiffness is a major cause of cardiovascular disease during normal aging and in Hutchinson–Gilford progeria syndrome (HGPS), a rare genetic disorder caused by ubiquitous progerin expression. This mutant form of lamin A causes premature aging associated with cardiovascular alterations that lead to death at an average age of 14.6 years. We investigated the mechanisms underlying vessel stiffness in LmnaG609G/G609G mice with ubiquitous progerin expression, and tested the effect of treatment with nitrites. We also bred LmnaLCS/LCSTie2Cre+/tgand LmnaLCS/LCSSM22αCre+/tg mice, which express progerin specifically in endothelial cells (ECs) and in vascular smooth muscle cells (VSMCs), respectively, to determine the specific contribution of each cell type to vascular pathology. We found vessel stiffness and inward remodeling in arteries of LmnaG609G/G609G and LmnaLCS/LCSSM22αCre+/tg, but not in those from LmnaLCS/LCSTie2Cre+/tgmice. Structural alterations in aortas of progeroid mice were associated with decreased smooth muscle tissue content, increased collagen deposition, and decreased transverse waving of elastin layers in the media. Functional studies identified collagen (unlike elastin and the cytoskeleton) as an underlying cause of aortic stiffness in progeroid mice. Consistent with this, we found increased deposition of collagens III, IV, V, and XII in the media of progeroid aortas. Vessel stiffness and inward remodeling in progeroid mice were prevented by adding sodium nitrite in drinking water. In conclusion, LmnaG609G/G609G arteries exhibit stiffness and inward remodeling, mainly due to progerin‐induced damage to VSMCs, which causes increased deposition of medial collagen and a secondary alteration in elastin structure. Treatment with nitrites prevents vascular stiffness in progeria. 相似文献
980.