Importance of the degree of antigen polymerization by detoxification in modulating the immunogenicity of acellular pertussis vaccine

For the acellular pertussis vaccine with a high immunogenicity, the concentration, composition and characteristics of acellular pertussis antigens are the crucial points to be considered. Nevertheless, it has not been proved yet whether or not the polymerization degree, one of the characteristics of formalin-detoxified acellular pertussis antigens, has an influence on vaccine potency. Thus, in the present study, the correlations among detoxification conditions of acellular pertussis bulks, their polymerization degrees and their immunogenicities were examined. In addition, the relative importance of pertussis toxoid in vaccine immunogenicity was also investigated. Results show that a lower lysine concentration during detoxification induces highly-polymerized antigens, the immunogenicity has a great dependency on the polymerization degree of antigens, and also pertussis toxoid has a relatively stronger influence on the immunogenicity than other antigens. Accordingly, in the aspect of the potency of detoxified acellular pertussis vaccine, it can be demonstrated that the polymerization of antigens and its degree are the major factors affecting the immunogenicity along with a relatively high content of pertussis toxoid.     

Influence of Parental Age on Endoreduplication of Giant Chromosomes and Some Quantitative Traits in Drosophila melanogaster Descendants

We studied the influence of parental age on the degree of polyteny of giant chromosomes and expressivity of mutation eyeless in Drosophila melanogaster descendants. The parental age equal to six days exerted an adverse effect on the function of endoreduplication of giant chromosomes in straineyeless. The highest degree of polyteny was observed in descendants of four- and ten-day imago. The maximum reduction of eye facets was observed in descendants of four-day imago, while in the progeny of older parents, the mutation expressivity was sharply reduced. Relations between the changes in chromosome polyteny, expressivity of mutation eyeless, and earlier studied components of adaptation were statistically analyzed in descendants of aging parents of this Drosophila strain.     

具有生育脉冲的Lotka-Volterra合作系统的正周期解的存在性

研究具有生育脉冲的两种群Lotka-Volterra合作系统,利用重合度理论,获得正周期解的存在的充分条件。     

在实验室条件下水稻螟蛾发育和存活的温度需求(英文)

对6种水稻害虫Chilo polychrysa (Meyrick)、C. suppressalis (Walker), C. partellus (Swinhoe), Scirpophaga incertulas (Walker), S. innotata (Walker)和Sesamia inferens的卵、幼虫和蛹在10到40°C的七个固定温度下(10,15,20,25 30,35和40°C)的发育和存活进行了研究。在一定的温度下6种害虫的卵、幼虫和蛹的发育周期是明显不同的(P<0.0001)。卵、幼虫和蛹的日发育的平均百分比随着温度的增加而增加。总发育周期与温度的增加成反比。临界温度下限是在10-15°C中间,而上限在35-40°C中间,此时害虫并不发育。对于上述6种害虫的卵、幼虫和蛹的平均发育起点温度分别为8.57±1.71,7.70±1.01,8.56±3.25,10.19±2.19,8.64±2.68和7.91±0.82°C。6种水稻害虫的卵、幼虫和蛹的总温度常数分别是705.56,725.32,703.30,556.59,655.34和837.95日·度。经计算,6种雌蛾的产卵所需的积温分别为99.06,90.85,99.29,75.16,92.25和80.41日·度。完成一世代所需的积温分别是804.62,816.17,802.59,631.75,648.84 and 918.36日·度。     

A new linear potentiometric titration method for the determination of deacetylation degree of chitosan

The degree of deacetylation (DD) is one of the most important properties of chitosan. Therefore, a simple, rapid and reliable method for the determination of DD of chitosan is essential. In this report, two new potentiometric titration functions are derived for the determination of DD of chitosan. The effects of the precipitation and the errors induced in pH measurement are discussed in detail. To make this method more simple and reliable, two universal pH regions for the accurate plotting of different DD chitosan samples are proposed for the new potentiometric titration functions. The DD values of three chitosan samples obtained with this new method show good agreement with those yielded from elemental analysis and ¹H-NMR.     

应用马尔可夫链法预测晚稻稻飞虱发生程度

根据化州市 1 979～ 1 999年 2 1年晚稻稻飞虱主害代发生程度的时间序列资料 ,应用马尔可夫链预测法对该市 2 0 0 0年和 2 0 0 1年稻飞虱发生程度进行了预测 ,结果与大田实际发生情况完全一致。对1 985～ 1 999年的历史资料进行回检 ,符合率为 96.7%,可对晚稻稻飞虱的发生程度进行超长期预测。     

通过自由能的差别划分蛋白质连续结构域

结构域是蛋白质三级结构下面的结构层次 ,大多数的蛋白质都可分为若干个结构域 ,结构域的不同组合使蛋白质具有不同的三级结构并具有不同的功能。蛋白质结构域的划分在理论与应用上都具有重要意义。但目前对结构域的划分还没有一个十分理想的方法。本文通过计算去折叠自由能来实现对蛋白质结构域的划分。用此方法对 5 0种不同蛋白质序列连续的双结构域进行了分析 ,大多数蛋白质的结构域划分结果与文献报道一致 ,另外一些虽然与文献报道结果不一致 ,但比文献报道的结果更合理     

Zum 70. Geburtstag Von Professor Dr. Habil. Theo Wetzel

Information on the structure of the population of Phomopsis species in Ukraine, that describes the structure of Phomopsis spp. cultivated on medium: the morfological characteristics, properties of pathogenicity and phytotoxicity. It is described the method of evaluation and selection of unsusceptible forms of sunflower to phomopsis. It includes the optimal infection level, the forms of an inoculum that is effective and optimal way for artificial infection.