排序方式: 共有158条查询结果,搜索用时 8 毫秒
91.
Biosorption of reactive dyes by the mycelium pellets of a new isolate of Penicillium oxalicum 总被引:4,自引:0,他引:4
Three reactive dyes were rapidly adsorbed by the mycelium pellets of Penicillium oxalicum. Dye removal of Reactive Blue 19 was up to 60% in 10 min and 91% in 80 min. Dye adsorption isotherms fitted Langmuir model well and the maximum adsorption capacities at 20 °C were calculated to be 160 mg g–1 for Reactive Blue 19, 122 mg g–1 for Reactive Red 241 and 137 mg g–1 for Reactive Yellow 145, respectively. The pellets exhibited a high dye adsorption capacity (80–180 mg g–1) for all of the 3 dyes over a wide pH range (pH 2–10), and the maximum adsorption was obtained at pH 2. The adsorption capacity was mildly increased by increasing salinity. 相似文献
92.
本研究从未成熟的有机蓝莓表皮分离、纯化得到一株白腐真菌G11,通过对菌株G11的形态特征、ITS序列同源性比对以及系统发育分析,鉴定菌株G11为一株烟管孔菌Bjerkandera adusta。菌株G11可以产生木质素过氧化物酶、漆酶和锰过氧化物酶等木质素降解酶。菌株G11对8种不同染料的脱色效果显示其对活性染料的脱色效果最好,脱色率达到90%所需时间最短。以菌株G11为研究对象,研究其对不同浓度的活性黑和活性红的脱色能力,结果表明:菌株G11对活性红和活性黑具有显著的脱色能力。在脱色15d时,菌株G11对浓度为10、50、100、250、500mg/L活性红的脱色率分别为99%、98%、95%、94%和92%;对浓度为10、50、100、250、500mg/L活性黑的脱色率分别为98%、97%、95%、93%和90%。 相似文献
93.
94.
从土壤中分离得到1株对阳离子蓝染料SD-GSL具有高效脱色能力的菌株ZHT-3,通过16S r DNA测序分析进行鉴定。以脱色率为指标,研究菌株ZHT-3对阳离子蓝SD-GSL的脱色性能,并考察pH、温度、振荡转速等因素对脱色率的影响,以及菌株的连续脱色能力。结果表明,菌株ZHT-3属于蜡状芽胞杆菌(Bacillus cereus)。该菌在对数生长阶段即可完成对阳离子蓝染料的快速脱色,8 h脱色率为76. 3%。对初始浓度100~500 mg/L阳离子蓝SD-GSL的36 h内脱色率高达95%以上。在pH5~8,温度30~40℃范围内脱色性能良好。 相似文献
95.
[目的]分离获得产漆酶的细菌菌株,研究漆酶的酶学性质并应用于染料脱色.[方法]利用含铜的富集培养基筛选产漆酶细菌;通过形态特征、生理生化试验及16SrDNA序列分析等方法进行鉴定;以丁香醛连氮为底物测定漆酶的酶学性质;通过测定染料在最大吸收波长下吸光值的变化评价漆酶对染料的脱色效果.[结果]从森林土壤中筛选到一株漆酶高产菌株LS05,初步鉴定为解淀粉芽孢杆菌(Bacillus amyloliquefaciens);菌株LS05的芽孢漆酶以丁香醛连氮为底物的最适pH为6.6,最适温度为70℃;该酶具有较好的稳定性,经70℃处理10h或在pH 9.0条件下放置10d后可保留活性.对抑制剂SDS和EDTA具有一定的抗性,在碱性条件下可有效脱色不同的工业染料,RB亮蓝、活性黑和靛红1h内的脱色率达93%以上.[结论]Bacillus amyloliquefaciens LS05的芽孢漆酶在高温和碱性条件下稳定性强,相对于真菌漆酶具有更好的工业应用特性,可有效用于工业染料废水的处理. 相似文献
96.
Emanuel GrassiPablo Scodeller Nestor FilielRomina Carballo Laura Levin 《International biodeterioration & biodegradation》2011,65(4):635-643
Trametes trogii BAFC 463 culture fluids (containing 110 U ml−1 laccase; 0.94 U ml−1 manganese peroxidase), as well as its purified laccase were capable of decolorizing azoic, indigoid, triphenylmethane, anthraquinonic and heterocyclic dyes, in the absence of redox mediators. Six dyes: RBBR, Indigo Carmine, Xylidine, Malachite Green, Gentian Violet and Bromophenol Blue were almost completely degraded (more than 85% decolorization after 1 d) by either laccase or T. trogii itself in culture, proving the role of the enzyme in dye decolorization. The purified laccase also decolorized 65% of Fast Blue RR and 30% of Azure B and Methylene Blue after 24 h. The use of redox mediators significantly increased the decolorization rates (90% decolorization of Azure B after 1 h). 1-hydroxybenzotriazole resulted the best redox mediator, but the natural mediator p-hydroxybenzoic acid also demonstrated its efficiency for dye decolorization. Due to their ability to decolorize recalcitrant dyes without addition of redox mediators, high laccase activities, high thermostability and efficient decolorization at 70 °C and pH 7.0, even in the presence of high concentrations of heavy metals (100 mM Cu+2, Pb+2 or Cd+2) or in a synthetic dyebath, T. trogii culture fluids could be effectively used to decolorize synthetic dyes from effluents. 相似文献
97.
胶红酵母JB401降解脱色三苯甲烷类染料 总被引:1,自引:0,他引:1
从烟梗中分离筛选得到1株能够对三苯甲烷类染料高效脱色的微生物,经ITS-5.8S rDNA分析鉴定为胶红酵母,命名为Rhodotorula mucilaginosa JB401。全波长扫描实验结果证实染料的脱色由胶红酵母降解结晶紫引起。为了提高R.mucilaginosa JB401脱色结晶紫的能力,通过单因素试验对R.mucilaginosa JB401的培养条件进行了优化,得出菌体生长24 h后以2%接种量接入初始pH为5的脱色培养基并在37℃摇床培养,可以取得最优脱色效果,此时脱色50、100和200 mg/L的结晶紫达到90%去除率分别需要3、6和14 h。此外,胶红酵母对温度和pH良好的适应性使其具有应用于工业废水处理的潜力。 相似文献
98.
99.
White-rot fungi (WRF) are capable of degrading complex organic compounds such as lignin, and the enzymes that enable these processes can be used for the detoxification of recalcitrant organopollutants. The aim of this study is to evaluate a system based on the use of an in vitro ligninolytic enzyme for the detoxification of recalcitrant dye pollutants. The dyes selected for investigation were the anionic and cationic commercial azo dyes, basic blue 41 (BB41), acid black 1 (AB1), and reactive black 5 (RB5). A supernatant, cell-free culture of WRF with manganese peroxidase activity was used to investigate its degradative capacity under various conditions, and concentrations of cofactors, H(2)O(2) and Mn(2+). The assays were carried out using a 2(2) experimental designs whose variables were concentration of Mn(2+) (33 and 1,000 μM) and semicontinuous dosage of the H(2)O(2) (0.02 and 0.10 μmol) added at a frequency of 0.2 min(-1). The response variables analyzed were the efficiency and the initial rate of the decolorization process. The dye concentrations considered ranged from 10 to 200 mg L(-1). AB1 and RB5 were decolorized over the entire interval of concentrations studied; reaching efficiencies between 15 and 95%. Decolorization of up to 100 mg L(-1), BB41 had less than 30% efficiency. The decay of the concentration of AB1 was interpreted by two-stage kinetics model, with the exception of the condition of 33 μM Mn(2+)-0.02 μmol of H(2)O(2) in which only one stage was observed. For all assays performed with 33 μM Mn(2+), the initial rate of the decolorization process was found to be dependent on the dosage of H(2)O(2). The results of this study can be applied to the development bioreactors for the degradation of recalcitrant pollutants from the textile industry and may be used as a model for expanding the use of extracellular enzyme supernatants in bioremediation. 相似文献
100.
Adriana ZillyJaqueline da Silva Coelho-Moreira Adelar BrachtCristina Giatti Marques de Souza Anne Elise CarvajalEloá Angélica Koehnlein Rosane Marina Peralta 《International biodeterioration & biodegradation》2011,65(2):340-344
In a solid state medium using yellow passion fruit waste as substrate, the basidiomycete Ganoderma lucidum produced a laccase as the main ligninolytic enzyme. This crude enzyme presented Michaelian behavior with both substrates tested, namely 3-ethylbenzthiazoline-6-sulphonic acid (ABTS) and the anthraquinone dye remazol brilliant blue R (RBBR). The KM’s for these substrates were, respectively, 0.232 × 10−3 and 0.602 × 10−3 M. The actions of NaCl and Na2SO4, two important salts usually found in textile wastewaters, were investigated. The enzyme was inhibited by NaCl, but not by Na2SO4. Inhibition by NaCl was of the mixed type with two different inhibition constants. The enzyme was able to completely decolorize RBBR in the presence of 1.0 M Na2SO4 and 50% decolorization was found in the presence of 0.1 M NaCl. Such properties certainly make the enzyme a good agent for textile dye effluent treatment considering the fact that wastewaters of this industry usually contain high concentrations of NaCl and Na2SO4. 相似文献