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71.
Amino acid release from roots of sterile and non-sterile, solution-grown, 7-, 21- and 60-days-old forage rape plants (Brassica napus L.), was measured over periods of up to 6 hours. With sterile plants, release of amino acids into a fixed volume of collection medium (6, 12, 70 mL) was concentration-limited, giving rise to similar convex accumulation profiles for individual acids. In contrast, amino acid accumulation in continuously circulating collection medium was not concentration limited, giving a linear accumulation pattern. The compositions of accumulating amino acids, which were similar to those measured in root extracts, did not change significantly. However, the proportions of ALA, GABA, GLU and ILE in both root extracts and root-derived amino acids increased as plants aged. Older plants released more amino acids per plant, while younger plants released more amino acids g-1 root DW. Using non-sterile plants, the patterns of change in amino acid concentration and composition in the collection medium were completely different from those determined with sterile plants. In general, with 7-days-old plants, and 60-days-old plants that had recently become non-sterile, an initial rise in the concentration of all acids was followed by a fall to low levels. The loss of amino acids was apparently due to microbial consumption. Individual amino acids attained maximum concentration at different times during the collection process. This is attributed mainly to concentration-dependent differential assimilation of amino acids, since those with the highest initial concentrations, the major components of the mixtures released from roots, declined the earliest. When calculated rates of amino acid release from roots (Rr) and microbial consumption of amino acids (Rc) were compared (for 7-days-old plants), the highest ratios of Rc/Rr were found for ASN, ARG, GLU, GLN, and LYS. This suggests a degree of selectivity for glutamate and nitrogen-rich acids on the part of the consuming micro-organisms. With 21-days old plants and 60-days old plants grown entirely under non-sterile conditions, fluctuations in amino acid concentration were similar for all acids. 相似文献
72.
Role of proteinaceous amino acids released in root exudates in nutrient acquisition from the rhizosphere 总被引:1,自引:1,他引:0
The role of proteinaceous amino acids in rhizosphere nutrient mobilization was assessed both experimentally and theoretically. The degree of adsorption onto the soil's solid phase was dependent on both the amino acid species and on soil properties. On addition of amino acids to both soil and freshly precipitated Fe(OH)3, no detectable mobilization of nutrients (K, Na, Ca, Mg, Cu, Mn, Zn, Fe, S, P, Si and Al) was observed, indicating a very low complexation ability of the acidic, neutral and basic amino acids. This was supported by results from a solution equilibria computer model which also predicted low levels of amino acid complexation with solutes present in the soil solution. On comparison with the Fe(OH)3 and equilibria data obtained for the organic acid, citrate, it was concluded that amino acids released into the rhizosphere have a limited role in the direct acquisition of nutrients by plants. The effectiveness of root exudates such as amino acids, phytosiderophores and organic acids in nutrient mobilization from the rhizosphere is discussed with reference to rhizosphere diffusion distances, microbial degradation, rate of complexation and the root's capacity to recapture exudate-metal complexes from the soil. 相似文献
73.
For the first time, an active fatty-acid metabolism is indicated for triacylglycerols (TAG) of developing sunflower (Helianthus annuus L.) seeds. When the developing seeds were transferred to low temperature, the total amount of oleate found in TAG decreased
as that of linoleate increased, while the contents of total lipids and TAG remained unchanged. These results suggest that
oleate from TAG was used for desaturation. This occurred first in microsomal TAG, but after a long cold period it was observed
mainly in the oil-body fraction. Thesn-2 position of TAG was preferentially enriched in linoleate. Apparently, more linoleate than necesary for the maintenance
of membrane fluidity was synthesized at the expense of TAG oleate. 相似文献
74.
Summary Homocysteine (HC) is a radiation protector but toxic to bone. Its derivative homocysteine thiolactone (HCTL) and the alpha-alkylated analogue (A-methyl-HCTL) was fed to mice for a period of six weeks in a daily dose of 50 mg/kg body weight. Parameters for bone matrix as collagen content, acid solubility of bone collagen, urinary bone collagen cross links (pyridinolines) and urinary acid glycosaminoglycans were determined. Urinary acid glycosaminoglycans were significantly reduced in the HCTL treated group but not in the alpha-methyl-homocysteine thiolactone (A-methyl-HCTL) group (controls: 45 ± 7 mg/mmol creatinine, homocysteine thiolactone 38 ± 5 mg/mmol creatinine, A-methyl HCTL 45 ± 6 mg/mmol creatinine).No differences were found for the parameters of bone collagen between the groups. The potent radiation protecting methylated derivative therefore did not change bone matrix and should be a candidate for further toxicological studies. 相似文献
75.
J. Garforth J. H. McKie R. Jaouhari T. J. Benson A. H. Fairlamb K. T. Douglas 《Amino acids》1994,6(3):295-299
Summary The rational design of ligands for the substrate-binding site of a homology-modelled trypanothione reductase (TR) was performed. Peptides were designed to be selective for TR over human glutathione reductase (GR). The design process capitalized on the proposed differences between the activesites of TR and human GR, subsequently confirmed by the TR crystal structure. Enzyme kinetics confirmed that forT. cruzi TR benzoyl-Leu-Arg-Arg-ß-naphthylamide was an inhibitor (Ki 13.8µM) linearly competitive with the native substrate, trypanothione disulphide, and did not inhibit glutathione reductase. 相似文献
76.
Summary The kinetics of putrescine and spermidine uptake and the influence of calcium on the kinetic parameters of the transport process were investigated in protoplasts isolated from carrot phloem parenchyma. Spermidine uptake dependence on external concentration was biphasic, both in the absence and in the presence of 1 mM CaCl2. In the first case, saturation was reached at 0.1 to 0.25 mM and the Km value was 43µM. When calcium was added, the Km and Vmax increased. A similar pattern was found with regard to putrescine uptake. Moreover, in order to clarify the mode of action of calcium on polyamine uptake, lanthanides (lanthanum and gadolinium) were utilised as Ca+2-channel antagonists. When protoplasts were preincubated with these lanthanides, the stimulatory effect exerted by Ca+2 on polyamine uptake was almost totally abolished. On the other hand, if lanthanum was supplied instead of calcium, it gave rise to a small enhancement of polyamine transport. These results induce us to suggest that calcium acts on polyamine uptake both by binding to external sites on the plasmalemma and by penetrating into the cell. 相似文献
77.
Summary Although the regulatory activity of steroid hormones on amino acid metabolism has been described, no information is published on the effect of ovariectomy. We studied the influence of ovariectomy in Wistar rats determining the amino acids phenylalanine and tyrosine in liver, kidney, plasma and urine. 32 animals were used in the study, 12 animals were sham operated, 9 animals were ovariectomized and 11 rats were ovariectomized and supplemented with estradiol. No quantitative changes were detected comparing liver and kidney phenylalanine and tyrosine between the groups (sham operated rats liver phenylalanine 2,53nM/mg ± 1,07; liver tyrosine 1.95nM/mg ± 0.92; kidney phenylalanine 2.16nM/mg ± 0.53; kidney tyrosine 1.80nM/mg ± 0.39. Ovariectomized rats showed liver phenylalanine 3.07nM/mg ± 1.14; liver tyrosine 2.63nM/mg ± 1.01; kidney phenylalanine 2.30 nM/mg ± 0.74; kidney tyrosine 1.93nM/mg ± 0.63. Ovariectomized and estradiol supplemented rats presented with liver phenylalanine 2.84nM/mg ± 1.40; liver tyrosine 2.35nM/mg ± 1.28; kidney phenylalanine 1.91nM/mg ± 0.26, kidney tyrosine 1.67nM/mg ± 0.23.). When, however, the phenylalanine/tyrosine ratio in the liver was evaluated, ovariectomized rats showed a significant decrease of the quotient (p = 0.001). The phenylalanine/tyrosine ratio was restored by estradiol replacement. Our findings show that phenylalanine and tyrosine metabolism is under estradiol control. The effect on the metabolic changes could be mediated by enzyme systems as phenylalanine hydroxylase, tyrosine hydroxylase and tyrosine aminotransferase. Our results would be compatible with previous reports on the stimulatory effect of estradiol on these enzymes. The kidney phenylalanine/tyrosine ratio was unaffected by ovariectomy and/or estradiol replacement which can be easily explained by different pools, enzyme activities, filtration/reabsorption effects, etc.The urinary P/T ratio was decreased by ovariectomy and restored by estradiol replacement indicating endocrine control of renal reabsorption and secretion mechanisms. 相似文献
78.
Summary Total D-amino acids were measured in plasma for 20 non-dialysed patients (creatinine clearance < 12 ml/minute), 20 on CAPD, 20 on haemodialysis and 20 normals. Plasma D-tyrosine and D-phenylalanine were measured in 8 of each group by HPLC. Total D-amino acids, D-tyrosine and D-phenylalanine were significantly greater for patients than normals. D-amino acids and D-tyrosine correlated with creatinine and were decreased during HD. During dialysis, the mean losses for D-tyrosine and D-phenylalanine were similar, about 0.2 mg/CAPD exchange and 3 mg/4 hour haemodialysis (i.e. 2% of the total amino acid, as in plasma). Clearance was unaffected by stereochemical configuration. Urinary losses/24 hour in the non-dialysed patients were 0.35 mg D-tyrosine and 0.25 mg D-phenylalanine. Clearance for D-phenylalanine was greater than for the L-enantiomer. Increases in D-amino acids in renal failure are probably due to depletion of D-amino acid oxidase, but may be enhanced by a D-amino acid rich diet, peptide antibiotics and D-amino acid oxidase inhibiting drugs and metabolites. Possible toxic effects need further investigation. 相似文献
79.
Summary The structure-activity data of 6 years on 395 analogs of the luteinizing hormone releasing hormone (LHRH) have been studied to determine effective substituents for the ten positions for maximal antiovulatory activity and minimal histamine release. The numbers of substituents studied in the ten positions are as follows: (41)1-(12)2-(12)3-(5)4-(47)5-(52)6-(16)7-(18)8-(4)9-(8)10. In position 1, DNal and DQal were effective with the former being more frequently the better substituent. DpClPhe was uniquely effective in position 2. Positions 3 and 4 are very sensitive to change. D3Pal in position 3 and Ser in position 4 of LHRH were in the best antagonists. PicLys and cPzACAla were the most successful residues in position 5 with cPzACAla being the better substituent. Position 6 was the most flexible and many substituents were effective; particularly DPicLys. Leu7 was most often present in the best antagonists. In position 8, Arg was effective for both antiovulatory activity and histamine release; ILys was effective for potency and lesser histamine release. Pro9 of LHRH was retained. DAlaNH2
10 was in the best antagonists.Abbreviations AABLys
N
-(4-acetylaminobenzoyl)lysine
- AALys
N
-anisinoyl-lysine
- AAPhe
3-(4-acetylaminophenyl)lysine
- Abu
2-aminobutyric acid
- ACLys
N
-(6-aminocaproyl)lysine
- ACyh
1-aminocyclohexanecarboxylic acid
- ACyp
1-aminocyclopentanecarboxylic acid
- Aile
alloisoleucine
- AnGlu
4-(4-methoxy-phenylcarbamoyl)-2-aminobutyric acid
- 2ANic
2-aminonicotinic acid
- 6ANic
6-aminonicotinic acid
- APic
6-aminopicolinic acid
- APh
4-aminobenzoic acid
- APhe
4-aminophynylalanine
- APz
3-amino-2-pyrazinecarboxylic acid
- Aze
azetidine-2-carboxylic acid
- Bim
5-benzimidazolecarboxylic acid
- BzLys
N
-benzoyllysine
- Cit
citrulline
- Cl2Phe
3-(3,4-dichlorphenyl)alanine
- cPzACAla
cis-3-(4-pyrazinylcarbonylaminocyclohexyl)alnine
- cPmACAla
cis-3-[4-(4-pyrimidylcarbonyl)aminocyclohexyl]alanine
- Dbf
3-(2-dibenzofuranyl)alanine
- DMGLys
N
-(N,N-dimethylglycyl)lysine
- Dpo
N
-(4,6-dimethyl-2-pyrimidyl)-ornithine
- F2Ala
3,3-difluoroalanine
- hNal
4-(2-naphthyl)-2-aminobutyric acid
- HOBLys
N
-(4-hydroxybenzoyl)lysine
- hpClPhe
4-(4-chlorophenyl)-2-amino-butyric acid
- Hse
homoserine, 2-amino-4-hydroxybutanoic acid
- ICapLys
N
-(6-isopropylaminocaproyl)lysine
- ILys
N
-isopropyllysine
- Ind
indoline-2-carboxylic acid
- INicLys
N
-isonicotinoyllysine
- IOrn
N
-isopropylornithine
- Me3Arg
NG,NG,NG-trimethylarginine
- Me2Lys
N
,N
-dimethyllysine
- MNal
3-[(6-methyl)-2-naphtyl]alanine
- MNicLys
N
-(6-methylpicolinoyl)lysine
- MPicLys
N
-(6-methylpicolinoyl)lysine
- MOB
4-methoxybenzoyl
- MpClPhe
N-methyl-3-(4-chlorphenyl)lysine
- MPZGlu
glutamic acid,-4-methylpiperazine
- Nal
3-(2-naphthyl)alanine
- Nap
2-naphthoic acid
- NicLys
N
-nicotinoyllysine
- NO2B
4-nitrobenzoyl
- NO2Phe
3-(4-nitrophenyl)alanine
- oClPhe
3-(2-chlorphenyl)alanine
- Opt
O-phenyl-tyrosine
- Pal
3-(3-pyridyl)alanine
- 2Pal
3-(2-pyridyl)alanine
- 2PALys
N
-(3-pyridylacetyl)lysine
- pCapLys
N
-(6-picolinoylaminocaproyl)lysine
- pClPhe
3-(4-chlorophenyl)alanine
- pFPhe
3-(4-fluorophenyl)-alanine
- Pic
picolinic acid
- PicLys
N
-picolinoyllysine
- Pip
piperidine-2-car-boxylic acid
- PmcLys
N
-(4-pyrimidylcarbonyl)lysine
- Ptf
3-(4-trifluromethyl phenyl)alanine
- Pz
pyrazinecarboxylic acid
- PzAla
3-pyrazinylalanine
- PzAPhe
3-(4-pyrazinylcarbonylaminophenyl)alanine
- Qal
3-(3-quinolyl)alanine
- Qnd-Lys
N
-quinaldoyllysine
- Qui
3-quinolinecarboxylic acid
- Qux
2-quinoxalinecarboxylic acid
- Tic
1,2,3,4-tetrahydroisoquinoline-3-carboxylic acid
- TinGly
2-thienylglycine
- tNACAla
trans-3-(4-nicotinoylaminocyclohexyl)-alanine
- tPACAla
trans-3-(4-picolinoylaminocyclohexyl)alanine 相似文献
80.
Summary The chirospecific conversions of D-glucosamine hydrochloride and D-mannosamine hydrochloride to the configurationally stable L and D isomers of N-t-butyloxycarbonylserinal were carried out byt-butylcarbonylation followed by sodium borohydride reduction and sodium meta-periodate oxidation. Reaction of the L and D aldehydes with the Wittig reagent prepared from 4-chlorobenzyltriphenylphosphonium chloride and butyl lithium followed by catalytic hydrogenation, Jones oxidation and salt formation with dicyclohexylamine gave the DCHA salts of the D and L isomers ofp-chlorohomophenylalanine N-t-Boc in high enatiomeric excess. The optical purity of the title compounds was established by hydrolysis to the respective free amino acids, followed by chiral derivatization and HPLC analysis.This was presented at the Fifth International Kyoto Conference on new Aspects of Organic Chemistry, Kyoto, Japan, November 11–15, 1991. Abstract #GO-13. 相似文献