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131.
Ngameni B Touaibia M Patnam R Belkaid A Sonna P Ngadjui BT Annabi B Roy R 《Phytochemistry》2006,67(23):2573-2579
A furanocoumarin glycoside new named turbinatocoumarin (1) was isolated from the twigs of Dorstenia turbinata. The structure of turbinatocoumarin (1) was assigned as 5-methoxy-3-[3-(beta-glucopyranosyloxy)-2-hydroxy-3-methylbutyl]psoralen by means of spectroscopic analysis. Known compounds have also been isolated from this genus and identified as (2'S, 3'R)-3'-hydroxymarmesin (2), 5-methoxy-3-(3-methyl-2,3-dihydroxybutyl)psoralen (3), psoralen (4), kanzonol C (5) which was isolated for the first time from this genus, 4-hydroxylonchocarpin (6), umbelliferone, 4-hydroxy-3-methoxybenzaldehyde and 4-methoxyphenol. As part of our continuing search for potential naturally-occurring antitumor drug candidates, the inhibition of matrix metalloproteinase (MMP)-2 secretion from brain tumor-derived glioblastoma cells by the isolated compounds 1, 3, 5, and 6 was evaluated by zymography and compared to the documented naturally-occurring MMP secretion inhibitors chlorogenic acid (CHL) and epigallocatechin-3-gallate (EGCg). Among the compounds tested, the inhibiting MMP secretion concentrations ranged from 0.025 to 250 microM with up to 80% inhibition. The inhibitory activities of compounds 5 and 6 were found comparable to the common reference compounds CHL and EGCg. This suggests that alternate sources can be explored and exploited for the availability of chemopreventive molecules. 相似文献
132.
Dong-Zhi Wei Ping Zou Mao-Bing Tu Hong Zheng 《Journal of Molecular Catalysis .B, Enzymatic》2002,18(4-6)
Ethyl glucoside lactate, a novel α-hydroxy acid derivative, was synthesized by transesterification in non-aqueous phase using immobilized lipase as biocatalyst. Parameters such as solvent type, substrate concentration, reaction temperature, and enzyme concentration were investigated to optimize the lipase-catalyzed transesterification. In solvent-free system with butyl lactate as both acyl donor and solvent, a 71% conversion was achieved. In order to investigate the effect of initial water content, the reactions were carried out in the mediums treated with molecular sieves. The results showed that conversion and initial rate decreased with the increase of water content. The conversion and initial rate reached to 95% and 67.4 mM/h, respectively, by carrying out the reaction under reduced pressure, which was employed to eliminate butanol and the initial water. 相似文献
133.
UDP-glucose:sterol glucosyltransferase: cloning and functional expression in Escherichia coli/ 总被引:4,自引:0,他引:4
Warnecke Dirk C. Baltrusch Martina Buck Friedrich Wolter Frank P. Heinz Ernst 《Plant molecular biology》1997,35(5):597-603
Steryl glucosides are characteristic lipids of plant membranes. The biosynthesis of these lipids is catalyzed by the membrane-bound UDP-glucose:sterol glucosyltransferase (EC 2.4.1.173). The purified enzyme (Warnecke and Heinz, Plant Physiol 105 (1994): 1067–1073) has been used for the cloning of a corresponding cDNA from oat (Avena sativa L.). Amino acid sequences derived from the amino terminus of the purified protein and from peptides of a trypsin digestion were used to construct oligonucleotide primers for polymerase chain reaction experiments. Screening of oat and Arabidopsis cDNA libraries with amplified labeled DNA fragments resulted in the isolation of sterol glucosyltransferase-specific cDNAs with insert lengths of ca. 2.3 kb for both plants. These cDNAs encode polypeptides of 608 (oat) and 637 (Arabidopsis) amino acid residues with molecular masses of 66 kDa and 69 kDa, respectively. The first amino acid of the purified oat protein corresponds to the amino acid 133 of the deduced polypeptide. The absence of these N-terminal amino acids reduces the molecular mass to 52 kDa, which is similar to the apparent molecular mass of 56 kDa determined for the purified protein. Different fragments of these cDNAs were expressed in Escherichia coli. Enzyme assays with homogenates of the transformed cells exhibited sterol glucosyltransferase activity. 相似文献
134.
Svasti J Phongsak T Sarnthima R 《Biochemical and biophysical research communications》2003,305(3):470-475
We have compared the ability of beta-glucosidases from cassava, Thai rosewood, and almond to synthesize alkyl glucosides by transglucosylating alkyl alcohols of chain length C(1)-C(8). Cassava linamarase shows greater ability to transfer glucose from p-nitrophenyl-beta-glucoside to secondary alcohol acceptors than other beta-glucosidases, and is unique in being able to synthesize C(4), C(5), and C(6) tertiary alkyl beta-glucosides with high yields of 94%, 82%, and 56%, respectively. Yields of alkyl glucosides could be optimized by selecting appropriate enzyme concentrations and incubation times. Cassava linamarase required pNP-glycosides as donors and could not use mono- or di-saccharides as sugar donors in alkyl glucoside synthesis. 相似文献
135.
Emmanuelle S. Briolat Mika Zagrobelny Carl E. Olsen Jonathan D. Blount Martin Stevens 《Journal of evolutionary biology》2019,32(1):31-48
Many defended species use conspicuous visual warning signals to deter potential predators from attacking. Traditional theory holds that these signals should converge on similar forms, yet variation in visual traits and the levels of defensive chemicals is common, both within and between species. It is currently unclear how the strength of signals and potency of defences might be related: conflicting theories suggest that aposematic signals should be quantitatively honest, or, in contrast, that investment in one component should be prioritized over the other, while empirical tests have yielded contrasting results. Here, we advance this debate by examining the relationship between defensive chemicals and signal properties in a family of aposematic Lepidoptera, accounting for phylogenetic relationships and quantifying coloration from the perspective of relevant predators. We test for correlations between toxin levels and measures of wing colour across 14 species of day‐flying burnet and forester moths (Lepidoptera: Zygaenidae), protected by highly aversive cyanogenic glucosides, and find no clear evidence of quantitative signal honesty. Significant relationships between toxin levels and coloration vary between sexes and sampling years, and several trends run contrary to expectations for signal honesty. Although toxin concentration is positively correlated with increasing luminance contrast in forewing pattern in 1 year, higher toxin levels are also associated with paler and less chromatically salient markings, at least in females, in another year. Our study also serves to highlight important factors, including sex‐specific trends and seasonal variation, that should be accounted for in future work on signal honesty in aposematic species. 相似文献
136.
Sex differences but no evidence of quantitative honesty in the warning signals of six‐spot burnet moths (Zygaena filipendulae L.)* 下载免费PDF全文
Emmanuelle Sophie Briolat Mika Zagrobelny Carl Erik Olsen Jonathan D. Blount Martin Stevens 《Evolution; international journal of organic evolution》2018,72(7):1460-1474
The distinctive black and red wing pattern of six‐spot burnet moths (Zygaena filipendulae, L.) is a classic example of aposematism, advertising their potent cyanide‐based defences. While such warning signals provide a qualitatively honest signal of unprofitability, the evidence for quantitative honesty, whereby variation in visual traits could provide accurate estimates of individual toxicity, is more equivocal. Combining measures of cyanogenic glucoside content and wing color from the perspective of avian predators, we investigate the relationship between coloration and defences in Z. filipendulae, to test signal honesty both within and across populations. There were no significant relationships between mean cyanogenic glucoside concentration and metrics of wing coloration across populations in males, yet in females higher cyanogenic glucoside levels were associated with smaller and lighter red forewing markings. Trends within populations were similarly inconsistent with quantitative honesty, and persistent differences between the sexes were apparent: larger females, carrying a greater total cyanogenic glucoside load, displayed larger but less conspicuous markings than smaller males, according to several color metrics. The overall high aversiveness of cyanogenic glucosides and fluctuations in color and toxin levels during an individual's lifetime may contribute to these results, highlighting generally important reasons why signal honesty should not always be expected in aposematic species. 相似文献
137.
Young-Min Kim Byung-Hoon Kim Joon-Seob Ahn Go-Eun Kim Sheng-De Jin Thanh-Hanh Nguyen Doman Kim 《Biotechnology letters》2009,31(9):1433-1438
Alkyl glucosides were synthesized by the reaction of Leuconostoc mesenteroides dextransucrase with sucrose and various alcohols. Alkyl α-d-glucosides were obtained with a yield of 30% (mol/mol) with primary alcohols, but secondary alcohols or tertiary alcohols
gave yields below 5%. The optimal yield was 50% using 1-butyl α-d-glucoside with 0.9 M 1-butanol. The acceptor products of methanol or ethanol were confirmed as methyl α-d-glucopyranoside and ethyl α-d-glucopyranoside via MALDI-TOF MS and NMR analysis. Thus, methyl or ethyl α-d-glucoside constituted half the emulsification activities of Triton X-100 as commercially available surfactants.
Young-Min Kim and Byung-Hoon Kim contributed equally to this work. 相似文献
138.
Endogenous pools of presumptive gibberellin (GA) glucosyl conjugates of Phaseolus coccineus were metabolically labelled by feeding of [3H]GA1 to immature fruits. The [3H]GA1 glucoside fraction was isolated and the main constituent tentatively identified by enzymic hydrolysis, ion exchange chromatography and elution volume on HPLC-RC as GA1-3-0--D-glucopyranoside. 相似文献
139.
Xuan-Xuan Zhou Qian Yang Yan-Hua Xie Ji-Yuan Sun Peng-Cheng Qiu Wei Cao Si-Wang Wang 《Phytochemistry letters》2013,6(3):372-378
Tetrahydroxystilbene glucoside (TSG) is a strong antioxidant and free radical scavenger derived from Polygonum multiflorum Thunb. The present study aims to evaluate the protective effect of TSG against d-galactose induced aging process in mice and its possible mechanisms of action. Our study revealed that administration of TSG improved the memory ability and regulated the body weight of mice. TSG also reduced the levels of ROS, NO and IGF-1 and increased the levels of SOD, Ca2+ and Klotho protein in the serum. Furthermore, TSG up-regulated the expression of Klotho protein in cerebrum, heart, kidney, testis and epididymis tissues of d-galactose induced aging mice. These results suggested that TSG had a promising anti-aging effect by regulating Klotho gene. 相似文献
140.
A new furan-2-carbonyl C-(6′-O-galloyl)-β-glucopyranoside (scleropentaside F, 1) and a new alkyl glucoside [butane-2,3-diol 2-(6′-O-galloyl)-O-β-glucopyranoside, 2] were isolated from the entire hemi-parasitic plant, Dendrophthoe pentandra growing on Tectona grandis together with ten known compounds including, benzyl-O-β-d-glucopyranoside (3), benzyl-O-α-l-rhamnopyranosyl-(1 → 6)-β-d-glucopyranoside (4), benzyl-O-β-d-apiofuranosyl-(1 → 6)-β-d-glucopyranoside (5), methyl gallate 3-O-β-d-glucopyranoside (6), methyl gallate 3-O-(6′-O-galloyl)-β-d-glucopyranoside (7), (+)-catechin (8), procyanidin B-1 (9) and procyanidin B-3 (10), bridelionoside A (11), and kiwiionoside (12). In addition, compounds 1, 3–9 were isolated from this species growing on the different host, Mangifera indica. The structure elucidations were based on physical data and spectroscopic evidence including 1D and 2D experiments. 相似文献