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71.
72.
S. Mtapuri-Zinyowera N. Midzi C.E. Muchaneta-Kubara T. Simbini T. Mduluza 《Journal of applied microbiology》2009,106(3):847-852
Aims: To determine the impact of natural sunlight in disinfecting water contaminated with cysts of Giardia duodenalis and Entamoeba histolytica/dispar using plastic containers.
Methods and Results: Known quantities of Giardia duodenalis and Entamoeba histolytica/dispar cysts in sterile water were exposed to the sun. Containers were made of polyethylene terephthalate, eight painted black on one side, one not painted and another cut open at the top and the last was a high density polypropylene container. Viability testing was performed using vital and fluorescent dyes. The same assays were conducted under cloudy conditions. Thermal control tests were also performed using heat without ultra violet light from the sun. Results show that 99·9% of parasites was inactivated when water temperatures reached 56°C after sunlight exposure.
Conclusion: Both solar radiation and heat produced by the sun have a synergistic effect in killing cysts of Giardia duodenalis and Entamoeba histolytica/dispar when temperatures rise above 50°C, with complete death at 56°C, using painted 2-l PET containers.
Significance and Impact of the Study: Solar disinfection system using PET containers painted black on one side can be used to disinfect water against Giardia duodenalis and Entamoeba histolytica/dispar using natural sunlight. 相似文献
Methods and Results: Known quantities of Giardia duodenalis and Entamoeba histolytica/dispar cysts in sterile water were exposed to the sun. Containers were made of polyethylene terephthalate, eight painted black on one side, one not painted and another cut open at the top and the last was a high density polypropylene container. Viability testing was performed using vital and fluorescent dyes. The same assays were conducted under cloudy conditions. Thermal control tests were also performed using heat without ultra violet light from the sun. Results show that 99·9% of parasites was inactivated when water temperatures reached 56°C after sunlight exposure.
Conclusion: Both solar radiation and heat produced by the sun have a synergistic effect in killing cysts of Giardia duodenalis and Entamoeba histolytica/dispar when temperatures rise above 50°C, with complete death at 56°C, using painted 2-l PET containers.
Significance and Impact of the Study: Solar disinfection system using PET containers painted black on one side can be used to disinfect water against Giardia duodenalis and Entamoeba histolytica/dispar using natural sunlight. 相似文献
73.
Aim To identify the factors that determine the geographical range sizes of ectoparasites with different degrees of host specificity.
Location The study used data on the distributions of fleas of the genus Amphipsylla and their rodent hosts across the Holarctic.
Methods All known points of occurrence of 32 flea species and 51 species of their rodent hosts were mapped. The shape and size of the geographical range of each species were estimated using a combination of the minimal convex polygon technique and modelling with the garp algorithm. Factors determining the geographical range sizes of the fleas were identified using stepwise multiple regression analysis.
Results The geographical range size of fleas that are strongly host-specific across their entire ranges correlated positively with the geographical range size of the fleas' principal hosts, and negatively with the geographical range size of the fleas' potential competitors. The geographical range sizes of both (1) fleas that are locally host-specific but that shift their host preferences geographically, and (2) host-opportunistic fleas were positively correlated only with the area of the geographical ranges of their principal hosts. Strongly host-specific fleas occupied 0.2–80.0% of the geographical range of their principal hosts, whereas this figure was 0.9–83.7% in locally host-specific fleas and 16.6–63.7% in host-opportunistic fleas.
Main conclusions The main determinant of the geographical range size of a flea species is the size of the geographical range of its hosts. The role of potential competitors in determining the geographical range size is stronger in host-specific than in host-opportunistic fleas. Cases in which the geographical range of a parasite is smaller than the geographical range(s) of its host(s) owing to narrower parasite environmental tolerances are much more frequent in host-opportunistic than in host-specific fleas. 相似文献
Location The study used data on the distributions of fleas of the genus Amphipsylla and their rodent hosts across the Holarctic.
Methods All known points of occurrence of 32 flea species and 51 species of their rodent hosts were mapped. The shape and size of the geographical range of each species were estimated using a combination of the minimal convex polygon technique and modelling with the garp algorithm. Factors determining the geographical range sizes of the fleas were identified using stepwise multiple regression analysis.
Results The geographical range size of fleas that are strongly host-specific across their entire ranges correlated positively with the geographical range size of the fleas' principal hosts, and negatively with the geographical range size of the fleas' potential competitors. The geographical range sizes of both (1) fleas that are locally host-specific but that shift their host preferences geographically, and (2) host-opportunistic fleas were positively correlated only with the area of the geographical ranges of their principal hosts. Strongly host-specific fleas occupied 0.2–80.0% of the geographical range of their principal hosts, whereas this figure was 0.9–83.7% in locally host-specific fleas and 16.6–63.7% in host-opportunistic fleas.
Main conclusions The main determinant of the geographical range size of a flea species is the size of the geographical range of its hosts. The role of potential competitors in determining the geographical range size is stronger in host-specific than in host-opportunistic fleas. Cases in which the geographical range of a parasite is smaller than the geographical range(s) of its host(s) owing to narrower parasite environmental tolerances are much more frequent in host-opportunistic than in host-specific fleas. 相似文献
74.
75.
Like any obligate intracellular pathogen, the parasite Toxoplasma gondii has lost its capacity for living independently of another organism. Toxoplasma lacks many genes that encode for entire metabolic pathways and has, in return, expanded genes that promote nutrient scavenging to meet its basic metabolic requirements. Although sequestrated in a parasitophorous vacuole and thus insulated from the nutrient-rich host cytosol and organelles by a membrane, T. gondii has evolved efficient strategies to acquire essential metabolites from mammalian cells. This review explores the natural auxotrophies and nutrient scavenging activities of the parasite, emphasising unique transport systems and salvage pathways. We describe the mechanisms deployed by Toxoplasma to modify its parasitophorous vacuole to gain access to host cytosolic molecules and to hijack host organelles to retrieve their nutrient content. From a therapeutic perspective, we survey the different possibilities to starve T. gondii by nutrient depletion or disruption of salvage pathways. 相似文献
76.
77.
The past 15 years have witnessed a number of molecular studies that aimed to resolve issues of species delineation and phylogeny
of mites in the family Tetranychidae. The central part of the mitochondrial COI region has frequently been used for investigating
intra- and interspecific variation. All these studies combined yield an extensive database of sequence information of the
family Tetranychidae. We assembled this information in a single alignment and performed an overall phylogenetic analysis.
The resulting phylogeny shows that important patterns have been overlooked in previous studies, whereas others disappear.
It also reveals that mistakes were made in submitting the data to GenBank, which further disturbed interpretation of the data.
Our total analysis clearly shows three clades that most likely correspond to the species T. urticae, T. kanzawai and T. truncatus. Intraspecific variation is very high, possibly due to selective sweeps caused by reproductive parasites. We found no evidence
for host plant associations and phylogeographic patterns in T. urticae are absent. Finally we evaluate the application of DNA barcoding. 相似文献
78.
79.
The root tuber peel of Flemingia vestita has been in use in local traditional medicine against intestinal worm infections in Meghalaya (North-East India). In order to evaluate and authenticate the anthelminitc efficacy of the isoflavones of F. vestita, the root peel extract of this putative plant was tested against several helminth parasites, extensively on Rallietina echinobothrida, with respect to different parameters of these parasites. In this paper, we describe various methods to evaluate the anthelmintic efficacy of this medicinal plant with respect to carbohydrate metabolism in R. echinobothrida at paralytic time caused by the isoflavones of F. vestita. To meet the high energy demand by the parasite due to the anthelmintic stress, glucose breakdown follows the PEPCK-malate pathway in the parasite. 相似文献
80.
Phylogenetic relationships of seven Haptoglossa isolates were analyzed by using mitochondrial COII amino acid sequences with a data set of 34 peronosporomycetes. Haptoglossa isolates formed a single clade and appeared to be basal to the clade consisting of all other peronosporomycetes. The Haptoglossa clade was divided into two subclades: one clade consisted of five aplanosporic isolates and the other included one aplanosporic
and one zoosporic isolate. These results indicate that the genus Haptoglossa is monophyletic, and patterns of infection cell formation reflect more the phylogenetic relationship between the species
than patterns of sporogenesis. 相似文献