全文获取类型
收费全文 | 37100篇 |
免费 | 2596篇 |
国内免费 | 5175篇 |
专业分类
44871篇 |
出版年
2024年 | 205篇 |
2023年 | 800篇 |
2022年 | 1066篇 |
2021年 | 1266篇 |
2020年 | 1228篇 |
2019年 | 1726篇 |
2018年 | 1413篇 |
2017年 | 1232篇 |
2016年 | 1184篇 |
2015年 | 1131篇 |
2014年 | 1843篇 |
2013年 | 2582篇 |
2012年 | 1472篇 |
2011年 | 1699篇 |
2010年 | 1357篇 |
2009年 | 1743篇 |
2008年 | 1724篇 |
2007年 | 1892篇 |
2006年 | 1649篇 |
2005年 | 1521篇 |
2004年 | 1310篇 |
2003年 | 1227篇 |
2002年 | 1119篇 |
2001年 | 849篇 |
2000年 | 781篇 |
1999年 | 715篇 |
1998年 | 702篇 |
1997年 | 610篇 |
1996年 | 522篇 |
1995年 | 512篇 |
1994年 | 495篇 |
1993年 | 454篇 |
1992年 | 421篇 |
1991年 | 439篇 |
1990年 | 332篇 |
1989年 | 329篇 |
1988年 | 336篇 |
1987年 | 256篇 |
1986年 | 304篇 |
1985年 | 490篇 |
1984年 | 599篇 |
1983年 | 341篇 |
1982年 | 474篇 |
1981年 | 458篇 |
1980年 | 418篇 |
1979年 | 312篇 |
1978年 | 226篇 |
1977年 | 243篇 |
1976年 | 212篇 |
1973年 | 179篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
101.
Long bone growth is analyzed for 180 children from a Medieval population at Kulubnarti in Sudanese Nubia (550–1450 A.D.). A regional interpopulation comparison is made with growth data from Wadi Halfa in Lower Nubia, and an intrapopulation analysis is undertaken to assess diachronic changes in growth at Kulubnarti. Changes in growth patterns are interpreted in the context of mortality and morbidity profiles and relationships between the three variables are discussed. It is suggested that changes in the sociopolitical environment may have been responsible in part for altering levels of biological stress and impacting growth. 相似文献
102.
103.
Kyungsook C. Kim Anthony H. Caswell J. -P. Brunschwig Neil R. Brandt 《The Journal of membrane biology》1990,113(3):221-235
Summary It has been previously recognized that a number of protocols may cause breakage of the triad junction and separation of the constituent organelles of skeletal muscle. We now describe a fraction of triad junctions which is refractory to the known protocols for disruption. Triads were passed through a French press and the dissociated organelles were separated on a sucrose density gradient, which was assayed for PN200-110, ouabain and ryanodine binding. Ryanodine binding showed a single peak at the density of heavy terminal cisternae. On the other hand, the PN200-110 and ouabain, which are external membrane ligands, bound in two peaks: one at the free transverse tubule region and the other at the light terminal cisternae. Similarly, a two peak pattern of PN200-110 and ouabain binding was observed when triad junctions were broken by the Ca2+-dependent protease, calpain, which selectively hydrolyzes the junctional foot protein. The light terminal cisternae vesicles were subjected to three different procedures of junctional breakage: French press, hypertonic salt treatment, and protease digestion using calpain or trypsin. The treated membranes were then centrifuged on density gradients. Only extensive trypsin digestion caused a partial shift of ouabain activity into the free transverse tubule region. These observations suggest that the triads are a composite mixture of breakage susceptible, weak, and breakage resistant, strong, triads. Scatchard analysis of PN200-110 suggests that the transverse tubules of strong triads contain a relatively high number of dihydropyridine receptors compared to those of weak triads. Thin section electron microscopic images of the strong triads comparable to those of intact muscle are presented. 相似文献
104.
Chromosome numbers for 26 different species of the generaPiper, Peperomia andPothomorphe (Piperaceae) are reported. The basic chromosome numbers are 2n = 26, x = 13 (Piper, Pothomorphe) and 2n = 22, x = 11 (Peperomia), polyploid series are characteristic forPiper andPeperomia. Piper has the smallest chromosomes and prochromosomal interphase nuclei,Peperomia the largest ones and mostly reticulate to euchromatic nuclei.Pothomorphe is intermediate in both characters. The karyomorphological differences betweenPothomorphe andPiper underline their generic separation. Interspecific size variation of chromosomes occurs inPiper andPeperomia. Infraspecific polyploidy was observed inPiper betle. C-banding reveals different patterns of heterochromatin (hc) distribution between the genera investigated. The genome evolution is discussed. 相似文献
105.
106.
Multivariate statistical analysis has been applied to time series measurements of aerosol elemental composition from PIXE
analysis of filter samples, and principal components have been resolved that represent distinct particle types in an external
mixture in the atmosphere. In this study, it is argued that a combination of chemical and statistical analyses of the data
may be more powerful in determining chemical species in atmospheric aerosols than studies that employ mainly direct chemical
analysis of chemical species in unresolved mixtures of aerosol particle samples. Sulfur is generally associated with mineral
dust elements. It is reasoned that the association may represent sulfuric acid coatings on particles that can lead to mineral
dissolution and solubilization of significant amounts of aluminum, iron, and other metals.
Upon wet or dry deposition to the surface, the fluxes of these metals in biologically-available form may be sufficient to
affect primary productivity in the world ocean and cause ecological damage in lakes. As a consequence, the fluxes of biogenic
trace gases to the atmosphere may be changed, possibly leading to changes in the tropospheric concentration of ozone. The
inputs to lakes of soluble aluminum, which is toxic to fish, may be partly by deposition directly from the atmosphere, thus
not limited to leaching of soils by acid deposition. Human inhalation of soluble aluminum and other solulilized mineral metals
may account, in part, for the observed geographic pattern of deaths attributed to chronic obstructive pulmonary disease (COPD)
that show high rates in cities of the Western US and the southeast region, but low in most of the midwest and northeast. 相似文献
107.
108.
Stanislav D. Zakharov Xia Li Taya P. Red'ko Richard A. Dilley 《Journal of bioenergetics and biomembranes》1996,28(6):483-494
The 8-kDa subunit c of theE. coli F0 ATP-synthase proton channel was tested for Ca++ binding activity using a45Ca++ ligand blot assay after transferring the protein from SDS-PAGE gels onto polyvinyl difluoride membranes. The purified subunit c binds45Ca++ strongly with Ca++ binding properties very similar to those of the 8-kDa CF0 subunit III of choloroplast thylakoid membranes. The N-terminal f-Met carbonyl group seems necessary for Ca++ binding capacity, shown by loss of Ca++ binding following removal of the formyl group by mild acid treatment. The dicyclohexylcarbodiimide-reactive Asp-61 is not involved in the Ca++ binding, shown by Ca++ binding being retained in twoE. coli mutants, Asp61Asn and Asp61Gly. The Ca++ binding is pH dependent in both theE. coli and thylakoid 8-kDa proteins, being absent at pH 5.0 and rising to a maximum near pH 9.0. A treatment predicted to increase the Ca++ binding affinity to its F0 binding site (chlorpromazine photoaffinity attachment) caused an inhibition of ATP formation driven by a base-to-acid pH jump in whole cells. Inhibition was not observed when the Ca++ chelator EGTA was present with the cells during the chlorpromazine photoaffinity treatment. An apparent Ca++ binding constant on the site responsible for the UV plus chlorpromazine effect of near 80–100 nM was obtained using an EGTA-Ca++ buffer system to control free Ca++ concentration during the UV plus chlorpromazine treatment. The data are consistent with the notion that Ca++ bound to the periplasimic side of theE. coli F0 proton channel can block H+ entry into the channel. A similar effect occurs in thylakoid membranes, but the Ca++ binding site is on the lumen side of the thylakoid, where Ca++ binding can modulate acid-base jump ATP formation. The Ca++ binding to the F0 and CF0 complexes is consistent with a pH-dependent gating mechanism for control of H+ ion flux across the opening of the H+ channel.This work was supported in part by grants from the Department of Energy and the U.S. Department of Agriculture.On leave from the Institute of Soil Science and Photosynthesis, Russian Academy of Science, Pushchino, Russia. 相似文献
109.
J. D. Paterson 《International journal of primatology》1996,17(4):585-611
I analyzed somatometric measurements from subsets of the Texas and Oregon transplanted troops of Japanese macaques(Macaca fuscata) to reveal secular changes in body size and shape. Body weights of the Texas population (N = 59) are lower than those of the
Oregon population(N = 49) and the founding population from Arashiyama. The adult weights of the Oregon population are significantly higher than the
founding population from Mihara. There are significant differences in adult circumferential measures and in skinfolds, which
are correlated with the increased weight of the Oregon macaques. The adult Texas macaques have longer limb segments in comparison
with the adult Oregon troop members, while the latter have significantly longer heads and trunks. Examination of the developing
morphological trends through regression analyses on the complete sample suggests distinctive growth patterns for each population.
Members of the Texas population start with smaller initial measurements but hold a steeper growth pattern for limb segments,
while the Oregon macaques start larger in most measures and show lower growth rates. I argue that these differences in both
somatometry and growth patterns are related to the differing climatic conditions under which the translocated macaques have
lived. This set of analyses supports the basic arguments for Bergmann’s rule and Allen’s rule. 相似文献
110.
Structural characterization of genes corresponding to cotton fiber mRNA, E6: reduced E6 protein in transgenic plants by antisense gene 总被引:13,自引:0,他引:13
Maliyakal E. John 《Plant molecular biology》1996,30(2):297-306
Two genes, each corresponding to fiber mRNA E6, were isolated from cotton cultivars Coker 312 (Gossypium hirsutum L.) and Sea Island (G. barbadense L.). E6 is one of the predominant fiber-specific mRNAs present during early fiber development. The distinguishing feature of the nucleotide-derived E6 protein is the presence of a motif where a dimer, Ser-Gly, is repeated several times. Two of the Sea Island genes contained a pentameric motif, Ser-Gly, while one of the Coker genes had one and the other had four motifs each. cDNA clones containing one or five Ser-Gly motifs were also identified. Thus, it appears that the strict conservation of this motif may not be critical to E6 protein function. Sequence characterizations of the genes and cDNAs showed that multiple members of the E6 family are transcribed in fiber and may result in proteins 238 to 246 amino acids long. The 3 ends of the genes and cDNAs showed considerable heterology among them. Transgenic plants containing antisense genes were generated to decipher E6 function. Transgenic cotton with reduced E6 protein levels in the range of 60 to 98% were identified. However, no discernible phenotypic changes in fiber development or properties were apparent. This result leads to the conclusion that E6 is not critical to the normal development or structural integrity of cotton fibers. 相似文献