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991.
The identification of collateral artery growth (arteriogenesis) as the only mechanism to compensate for the loss of an occluded
artery forced us to define the mechanisms responsible for this type of vessel growth. To achieve this, a variety of coronary
as well as peripheral models of arteriogenesis have been developed. Based on these studies it is obvious that arteriogenesis
obeys different mechanisms than angiogenesis, the sprouting of capillaries. Upon occlusion of an artery, the blood flow is
redirected into preexisting arteriolar anastomoses that experience increased mechanical forces such as shear stress and circum
ferential wall stress. The endothelium of the arteriolar connections is then activated, resulting in an increased release
of monocyte-attracting proteins as well as an upregulation of adhesion molecules. Upon adherence and extravasation, monocytes
promote arteriogenesis by supplying growth factors and cytokines that bind to receptors that are expressed on vascular cells
within a limited time frame. Animal studies evidenced that factors, such as monocyte chemoattractant protein-1, granulocyte-monocyte
colony-stimulating factor, or transforming growth factor-β1, that either attract or prolong the lifetime of monocytes efficiently enhance collateral artery growth, an effect that was
seen only to a minor degree after application of a single growth factor. Bone marrow-derived stems cells and endothelial progenitor
cells do not incorporate in growing arteries but, rather, function as supporting cells. Complete elucidation of the mechanisms
of arteriogenesis may lead to efficacious therapies counteracting the devastating consequences of vascular occlusive diseases. 相似文献
992.
993.
994.
Simko F Pelouch V Torok J Luptak I Matuskova J Pechanova O Babal P 《Journal of biomedical science》2005,12(1):103-111
The aim of this study was to determine whether protein remodeling of the heart ventricles and remodeling of the aorta were present in hereditary hypertriglyceridemic (hHTG) rats and whether treatment with the angiotensin-converting enzyme inhibitor, captopril could prevent these alterations. Three groups of rats were investigated in a four week experiment control Wistar /C/rats, hHTg rats, hHTg rats given captopril (100 mg/kg/day) (hHTg + CAP). In the hHTg group, the increased systolic blood pressure (SBP) was associated with hypertrophy of the LV and RV. Protein profile analysis revealed an enhancement of metabolic protein concentration in both ventricles. The concentration of total collagenous proteins was not changed in either ventricles. However, alterations in composition of cardiac collagen were detected, characterized by higher concentration of hydroxyproline in pepsin-insoluble fraction and lower concentration of hydroxyproline in pepsin soluble faction in the LV. Hypertrophy of aorta, associated with the reduction of nitric oxide dependent relaxation, was also present in hHTG rats. Captopril normalized SBP, reduced left ventricular hypertrophy (LVH), diminished metabolic protein concentration in both ventricles, and improved NO-dependent relaxation of the aorta. Furthermore, captopril partially reversed alterations in hydroxyproline concentration in soluble and insoluble collagenous fractions of the LV. We conclude that hypertrophy of both ventricles and the aorta are present in hHTG rats, along with protein remodeling of both ventricles. Captopril partially prevented left ventricular hypertrophy development and protein remodeling of the myocardium. 相似文献
995.
Xylans are known to be major cellulose-linking polysaccharides in secondary cell walls in higher plants. We used two monoclonal antibodies (LM10 and LM11) for a comparative immunocytochemical analysis of tissue and cell distribution of xylans in a number of taxa representative of all major tracheophyte and bryophyte lineages. The results show that xylans containing the epitopes recognized by LM10 and LM11 are ubiquitous components of secondary cell walls in vascular and mechanical tissues in all present-living tracheophytes. In contrast, among the three bryophyte lineages, LM11 binding was detected in specific cell-wall layers in pseudoelaters and spores in the sporophyte of hornworts, while no binding was observed with either antibody in the gametophyte or sporophyte of liverworts and mosses. The ubiquitous occurrence of xylans containing LM10 and LM11 epitopes in tracheophytes suggests that the appearance of these polysaccharides has been a pivotal event for the evolution of highly efficient vascular and mechanical tissues. LM11 binding in the sporophyte of hornworts, indicating the presence of relatively highly substituted xylans (possibly arabinoxylans), separates these from the other bryophytes and is consistent with recent molecular data indicating a sister relationship of the hornworts with tracheophytes. 相似文献
996.
Tomoyuki Kakugawa Hiroshi Mukae Tomayoshi Hayashi Hiroshi Ishii Seiko Nakayama Noriho Sakamoto Sumako Yoshioka Kanako Sugiyama Mariko Mine Yohei Mizuta Shigeru Kohno 《Respiratory research》2005,6(1):57
Background
Heat shock protein (HSP) 47, a collagen-specific molecular chaperone, is involved in the processing and/or secretion of procollagens, and its expression is increased in various fibrotic diseases. The aim of this study was to determine whether quantitative immunohistochemical evaluation of the expression levels of HSP47, type I procollagen and α-smooth muscle actin (SMA) allows the differentiation of idiopathic usual interstitial pneumonia (UIP) from UIP associated with collagen vascular disease (CVD) and idiopathic nonspecific interstitial pneumonia (NSIP).Methods
We reviewed surgical lung biopsy specimens of 19 patients with idiopathic UIP, 7 with CVD-associated UIP and 16 with idiopathic NSIP and assigned a score for the expression of HSP47, type I procollagen and α-SMA in type II pneumocytes and/or lung fibroblasts (score 0 = no; 1 = weak; 2 = moderate; 3 = strong staining).Results
The expression level of HSP47 in type II pneumocytes of idiopathic UIP was significantly higher than in CVD-associated UIP and idiopathic NSIP. The expression of HSP47 in fibroblasts was significantly higher in idiopathic UIP and idiopathic NSIP than in CVD-associated UIP. The expression of type I procollagen in type II pneumocytes was significantly higher in idiopathic UIP than in idiopathic NSIP. The expression of type I procollagen in fibroblasts was not different in the three groups, while the expression of α-SMA in fibroblasts was significantly higher in idiopathic UIP than in idiopathic NSIP.Conclusion
Our results suggest the existence of different fibrotic pathways among these groups involved in the expression of HSP47 and type I procollagen. 相似文献997.
高原鼠兔肺动脉血管功能及形态变化 总被引:9,自引:1,他引:8
Chen QH 《中国应用生理学杂志》2001,17(2):178-181
目的研究肺循环对慢性缺氧的适应机理.方法在4300m的高度捕捉到高原适应动物鼠兔,带到2260m的高度并和10只Wistar大鼠在模拟4300m和5000m高度的低压仓内进行了肺动脉压的测定,观察肺组织学和组织免疫化学的改变.结果在2260m,鼠兔的Ppa明显低于Wistar大鼠,二者分别为(1.5±0@07)kPa和(2.9±1.1)kPa(P<0.01).随着海拔高度的增加,鼠兔的Ppa上升不明显,而Wistar大鼠增加显著.左右心室比重鼠兔为0.22,而Wistar大鼠为0.45.鼠兔的Hb,Hct和2.3-DPG均低于大鼠.大鼠肺小血管周围可见肥大细胞(7.1±0.33)mm2,免疫组化染色mastcelltyptase颗粒呈阳性,鼠兔未发现肥大细胞及此种免疫反应.肺小动脉中层较鼠兔厚,分别为27.21%和9.22%,壁的厚度和Ppa有很好的(r=0.763).结论鼠兔无低氧性肺血管收缩,是一种遗传性适应.大鼠肥大细胞通过激活某些生长因子,在肺血管的再建过程中可能起一定作用. 相似文献
998.
Solution structure of a phage-derived peptide antagonist in complex with vascular endothelial growth factor 总被引:3,自引:0,他引:3
Pan B Li B Russell SJ Tom JY Cochran AG Fairbrother WJ 《Journal of molecular biology》2002,316(3):769-787
Vascular endothelial growth factor (VEGF) is a potent endothelial cell-specific mediator of angiogenesis and vasculogenesis. VEGF is involved pathologically in cancer, proliferative retinopathy and rheumatoid arthritis, and as such represents an important therapeutic target. Three classes of disulfide-constrained peptides that antagonize binding of the VEGF dimer to its receptors, KDR and Flt-1, were identified previously using phage display methods. NMR studies of a representative peptide from the most potent class of these peptide antagonists, v107 (GGNECDAIRMWEWECFERL), were undertaken to characterize its interactions with VEGF. v107 has no defined structure free in solution, but binding to VEGF induces folding of the peptide. The solution structure of the VEGF receptor-binding domain-v107 complex was determined using 3940 (1970 per VEGF monomer) internuclear distance and 476 (238 per VEGF monomer) dihedral angle restraints derived from NMR data obtained using samples containing either (13)C/(15)N-labeled protein plus excess unlabeled peptide or (13)C/(15)N-labeled peptide plus excess unlabeled protein. Residual dipolar coupling restraints supplemented the structure determination of the complex and were found to increase significantly both the global precision of VEGF in the complex and the agreement with available crystal structures of VEGF. The calculated ensemble of structures is of high precision and is in excellent agreement with the experimental restraints. v107 has a turn-helix conformation with hydrophobic residues partitioned to one face of the peptide and polar or charged residues at the other face. Contacts between two v107 peptides and the VEGF dimer are mediated by primarily hydrophobic side-chain interactions. The v107-binding site on VEGF overlaps partially with the binding site of KDR and is similar to that for domain 2 of Flt-1. The structure of the VEGF-v107 complex provides new insight into how binding to VEGF can be achieved that may be useful for the design of small molecule antagonists. 相似文献
999.
Extensive evidence suggests that reactive oxygen species are critically involved in the pathogenesis of cardiovascular diseases, such as atherosclerosis and myocardial ischemia-reperfusion injury. Consistent with this concept, administration of exogenous antioxidants has been shown to be protective against oxidative cardiovascular injury. However, whether induction of endogenous antioxidants by chemical inducers in vasculature also affords protection against oxidative vascular cell injury has not been extensively investigated. In this study, using rat aortic smooth muscle A10 cells as an in vitro system, we have studied the induction of cellular antioxidants by the unique chemoprotector, 3H-1,2-dithiole-3-thione [corrected] (D3T) and the protective effects of the D3T-induced cellular antioxidants against oxidative cell injury. Incubation of A10 cells with micromolar concentrations of D3T for 24 h resulted in a significant induction of a battery of cellular antioxidants in a concentration-dependent manner. These included reduced glutathione (GSH), GSH peroxidase, GSSG reductase, GSH S-transferase, superoxide dismutase, and catalase. To further examine the protective effects of the induced endogenous antioxidants against oxidative cell injury, A10 cells were pretreated with D3T and then exposed to either xanthine oxidase (XO)/xanthine, 4-hydroxynonenal, or cadmium. We observed that D3T pretreatment of A10 cells led to significant protection against the cytotoxicity induced by XO/xanthine, 4-hydroxynonenal or cadmium, as determined by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium reduction assay. Taken together, this study demonstrates for the first time that a number of endogenous antioxidants in vascular smooth muscle cells can be induced by exposure to D3T, and that this chemical induction of cellular antioxidants is accompanied by markedly increased resistance to oxidative vascular cell injury. 相似文献
1000.