Summary The effect of a conventional antibiotic (penicillin/streptomycin) mixture on the widely used kidney epithelial cell line,
LLC-PK1, was investigated by measuring growth and intracellular free calcium. Free calcium concentration was the same in cells cultured
for 3 to 7 wk with (“plus”) and without (“minus”) antibiotics both at rest and when challenged with high (14 mM) external calcium. When exposed to vasopressin, minus cells exhibited significantly smaller calcium transients than plus
cells. A similar difference existed for transients elicited by a calcium ionophore, 4-br-A23187. After longer periods of culture
(>20 wk), minus cells grew slower than plus cells but on reaching confluence (minus cells took 1 day longer) the morphologies
and viabilities were indistinguishable. The finding that culture with penicillin/streptomycin reversibly modified some properties
of LLC-PK1 cells, at least partly through altered calcium homeostasis, is of importance for workers using this cell model to study drug
effects and raises the general possibility of similar effects on other cultured cells. 相似文献
Recent progress in plant transformation for insect resistance has increased the interest in the potential toxicity of proteins
towards insect pests. While studies have been targeted to a large array of insect species, phloem-feeding Homoptera have not
been investigated yet. The paper describes a routine test for screening toxicity and growth inhibition of purified proteins
in artificial diets onAcyrthosiphon pisum (Harris). Twenty-five commercially available proteins of different classes were tested and compared to some non-protein chemicals
(an insecticide, an antibiotic …).A. pisum proved to be very sensitive to all proteases tested and to some venoms with general cytolytic properties. A plant lectin,
concanavalin A, displayed significant toxicity and growth inhibition, while various proteins such as a soybean proteinase
inhibitor, a chitinase, and bovine serum albumin showed measurable impairments of growth only at higher dose (≥250 μg.ml−1). Some proteins were without short-term effect onA. pisum physiology. The influence of these results on aphid-plant interactions are discussed.
Résumé L'effet de protéines alimentaires sur les insectes phloémophages, dont les pucerons, n'a jamais été étudié. Nous proposons
ici un test biologique standardisé sur milieu artificiel permettant d'analyser les effets de différentes classes de protéines
sur la physiologie d'A. pisum. La validité de ce test est éprouvée (protocole, reproductibilité) et les différentes données récoltées (mortalité et inhibition
de croissance) permettent de définir des paramètres toxicologiques tels que concentration létale 50 ou concentration inhibitrice
50. Cette caractérisation toxicologique a été réalisée sur 25 protéines appartenant à des classes différentes, ainsi que plusieurs
substances non protéiques utilisées comme témoin de toxicité (insecticide, antibiotique, inhibiteur de synthèse protéique
et glucoside phénolique). Les regroupements de protéines par proximités de profils toxicologiques ont été corrélés aux activités
biochimiques des différentes protéines. Les implications de ces résultats sur les interactions plante-puceron sont discutées,
ainsi que le potentiel d'une stratégie de création de variétés transgéniques résistantes aux pucerons.
The effects on mitochondrial respiratory parameters of heavy metals, such as Cu, Ni, Pb, Cd, Zn, Ag, Hg, were recorded by using thein vitro response of submitochondrial particles (SMP) from beef heart mitochondria.The toxicity of these elements was estimated by determining their effects on the energy-coupled reverse electron transfer (RET), which is induced by ATP and succinate at first site level of the respiratory chain in SMP.The RET rate was easily monitored by recording spectrophotometrically at 340 nm the production of NADH, arising from the reduction of exogenous NAD+ by RET.The toxicity values were expressed as the toxicant molar concentration which decreases the rate of reduction of NAD+ to an extent of 50 percent (EC50). The toxicity increased in the following order: Ni2+2+2+< Cd2+2+2++.The SMP data were compared with the toxicity values obtained from a variety of biological systems currently used for toxicity testing. The results obtained demonstrate that the SMP test generally provides a good estimate of metal toxicity for several fish and invertebrate species. This is demonstrated by the statistical parameters obtained in the regression analysis. The broadened 95% confidence intervals and, in particular, the poor correlations obtained for some aquatic organisms can be ascribed to the more complex metabolic interactions and competing toxic pathways in aquatic organisms, when compared to SMP. 相似文献
Summary Iron tolerance of rice (Oryza sativa L.) was investigated using an oxygen depleted hydroculture system. Treatment with high concentrations of Fe2+ induced yellowing and bronzing symptoms as well as iron coatings at the root surface. Root and shoot growth were inhibited by increasing iron concentration in the medium. All symptoms were more pronounced in an iron sensitive cultivar (IR 64) compared to an iron tolerant one (IR 9764-45-2). Superoxide dismutase and peroxidase activity of root extracts of IR 97 were about twice that of IR 64 in untreated control plants. No significant increase of peroxidase activity was detected with increasing iron concentration in the medium. Catalase activity of IR 64 was slightly higher than that of IR 97, independent of iron concentration.Abbreviations SOD
Superoxide dismutase (EC 1.15.1.1)
- POD
peroxidase (EC 1.11.1.7)
- EDTA
ethylenediamintetraacetic acid
- fwt
fresh weight
- Hepes
(N-[2-hydroxyethyl]piperazine-N-[2-ethanesulfonic acid])
- BSA
bovine serum albumin
- IR 97 IR 9764-45-2
an iron tolerant rice cultivar
- IR 64
iron sensitive rice cultivar
- PM
plasma membrane 相似文献
The 1H-NMR spectrum of cucumber basic blue protein (CBP) has been recorded. Examination of the spectrum of the reduced protein suggests that one or more sidechains exist in conformations which interconvert slowly at ambient temperatures. His 39, His 84 and Met 89 are identified as copper ligands by redox titration and by amino acid sequence homology with plastocyanin and azurin. The importance of a Phe sidechain close to the Met ligand in the potential blue copper site is confirmed. Broadening of His ligand resonances at elevated temperatures reveals an exchange process at the reduced copper centre. 相似文献
Thyroid hormones are involved in copper and zinc distribution in rat tissues. We examined the influence of thyroparathyroidectomy
(TPTY) and of a replacement therapy by T4 on Cu and Zn organ distribution. MT levels were also measured both in basal conditions
and after induction by cadmium. The results confirm that a lack of T4 modified Cu and Zn in serum and tissues. In serum, TPTY
increased Cu (+15%) and ceruloplasmin (+18%), and decreased Zn (−18%). In tissues, Cu was altered in liver (+13%), kidney
(−24%), heart (−16%) duodenum (−18%), and Zn in liver (+25%) and kidney (−10%). The soluble fractions (100,000 g supernatant)
were mainly affected in liver and kidney, and the subcellular fractions in heart and duodenum. MT levels were modified in
basal conditions only in liver (+57%) and kidney (−36%). T4 administration partially prevented the effect of TPTY on both
elements and MT concentrations. Therefore, no evidence is provided for a direct role of T4 in the metabolism of MT in a way
comparable to the effects of glucocorticoids. However, MT could mediate the consequences of TPTY on metal distribution in
certain organs, such as liver and kidney. 相似文献
The toxicity of an Alamine 336/oleyl-alcohol extraction system on Lactobacillus delbrueckii was investigated. It was shown that the solvent affected the cells through the water-soluble portion and the immiscible portion of the solvent. While immobilization significantly protected the cells from the immiscible solvent phase, the water-soluble part of the solvent still caused toxicity to the microorganisms due to diffusion of the solvent into the matrix. Adding soybean oil to the kappa-carrageenan matrix could trap the diffusing solvent molecules, and therefore reduce the toxic effect from the water soluble portion of the solvent. The protective ability of soybean oil was quantified through mathematical modeling and experimentation. 相似文献
MTBE is a colorless, relatively volatile liquid that has found widespread use as an octane‐enhancing gasoline additive. In 1987, the Environmental Protection Agency's (EPA) Interagency Testing Committee identified MTBE for priority testing consideration based on large production volume, potential widespread exposure, and limited data on chronic health effects. In response, the industry formed the MTBE Health Effects Testing Task Force, which in 1988 signed a Consent Agreement with the EPA requiring the task force member companies to perform toxicological testing on MTBE.
The testing program, which began in the second quarter of 1988, consists of a full complement of short‐ and long‐term tests. The testing completed to date includes genotoxicity (in vivo bone marrow cytogenetics and Drosophila sex‐linked recessive lethal assays), developmental toxicity, acute and subchronic neurotoxicity (motor activity, functional observation battery, and neuropathology), subchronic toxicity, reproductive/fertility effects, and pharmacokinetic studies. There is also an ongoing oncogenicity study in rats and mice. The final report for this chronic study is expected at the end of 1992. The total cost for the program is approximately $3.75 million, which is funded by the 11 Task Force member companies based on market share.
These studies were sponsored by the MTBE Health Effects Testing Task Force, Oxygenated Fuels Association, Washington, D.C. 相似文献
Leukocytes incubated with Cu(II) showed a decrease in both glutathione reductase activity and reduced glutathione content.
The glucose 6-phosphate dehydrogenase activity under the same conditions was not affected. Serum albumin added to mixtures
prevented the loss of enzyme activity, whiled-penicillamine andl-histidine had little effect. Prior oxidation of the cell-reduced glutathione did not diminish the enzyme inhibitory action
of Cu(II). The amount of regeneration of reduced glutathione in leukocytes previously treated with diamide to oxidize their
reduced glutathione was a function of Cu(II) concentration in the media. No evidence was obtained that elevated serum ceruloplasmin
levels in rabbits, nor incubation of leukocytes in vitro with ceruloplasmin, affect leukocyte glutathione reductase activity.
It was proposed that the major mechanism by which copper affects glutathione metabolism in leukocytes is by inhibition of
glutathione reductase. 相似文献
The red marine algae Microcladia borealis, M. californica and M. coulteri produce several unusual halogenated monoterpenes including violacene, plocamene-B, plocamene-C, and plocamane-D. The isolation of these terpenes along with a study of their variation in each Microcladia at different locations are described. 相似文献