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351.
《Expert review of proteomics》2013,10(3):373-385
The latest advances in combinatorial peptide ligand libraries, with their unique performance in discovering low-abundance species in proteomes, are reviewed here. Explanations of mechanism, potential applications, capture of proteomes at different pH values to enhance the total catch and quantitative elutions, such as boiling in the presence of 5% sodium dodecyl sulfate and 3% dithiothreitol are included. The reproducibility of protein capture among different experiments with the same batch of beads or with different batches is also reported to be very high, with coefficient of variations in the order of 10–20%. Miniaturized operations, consisting of capture with as little as 20 or even 5 µl of peptide beads are reported, thus demonstrating that the described technology could be exploited for routine biomarker discovery in a biomedical environment. Finally, it is shown that the signal of captured proteins is linear over approximately three orders of magnitude, ranging from nM to µM, thus ensuring that differential quantitative proteomics for biomarker discovery can be fully implemented, providing species do not saturate their ligands. 相似文献
352.
We describe an approach to isolate molecular scaffolds and R-groups from known chemical compounds in order to generate scaffold and R-group databases from two large compound collections, OptiverseTM and MaybridgeTM. The distributions of molecular scaffolds and R-groups in the parent databases were analysed and compared. We find that a limited number of scaffolds and R-groups account for the majority of database compounds and that most of the scaffolds occur only once or twice in the compound databases. Diversity analysis suggests that the compound and scaffold databases have similar molecular diversity. Implications for library design are discussed.Electronic Supplementary Material available. 相似文献
353.
Broadbridge Robert J. Sharma Ram P. 《International journal of peptide research and therapeutics》1999,6(5-6):335-341
Summary Phosphorylation reactions are key meditors in a variety of biochemical signal processes. Research into the selective inhibition
of protein tyrosine kinases to generate anticancer agents has madeO-phosphotyrosyl analogues important pharmacological tools. The simple procedures reported here involving the formation of
interative peptide libraries together with the development of a selective and sensitive bead-binding assay have made it possible
to rapidly screen peptides incorporatingO-phosphotyrosyl surrogates (includingO-phospho-2,3,5,6-tetrafluorotyrosine, 4-(phosphono)hydroxymethyl-phenylalanine and 4-(phosphono)fluoromethyl-phenylalanine)
for their potential to inhibit the protein tyrosine kinase p56lck. These procedures can be easily adapted to combinatorical peptide libraries. 相似文献