动物离心机中大鼠行为状态无线视频采集装置的研制

目的：研制一种在正加速度作用下能实时观测大鼠行为状态的实验视频采集装置,为研究正加速度导致脑损伤的病理生理机制和防治措施提供新的方法。方法：依据正加速度对大鼠体位的要求和离心机特殊的环境,通过特制的大鼠座舱和无线视频采集单元来实现对动物离心机中正加速度作用下大鼠行为状态的实时观测;固定大鼠的座舱主要由胸部固定圈、身长调节圈、头部运动限制框构成;无线视频采集单元由2.4G无线摄像头、无线信号接收器、信号放大器、计算机以及视频采集软件组成。结果：座舱特殊的大鼠固定方式,既满足了正加速度对体位的要求,又不影响对大鼠行为状态的观测,2．4G无线视频采集技术保证视频信号在离心机高速旋转环境下的实时传输和观测,视频画面清晰流畅,并且能保存视频供回放分析。结论：动物离心机无线视频采集装置可用于正加速度作用下大鼠行为状态的实时观测与研究。     

Collection and production of native seeds for ecological restoration

The global push to achieve ecosystem restoration targets has resulted in an increased demand for native seeds that current production systems are not able to fulfill. In many countries, seeds used in ecological restoration are often sourced from natural populations. Though providing seed that is reflective of the genetic diversity of a species, wild harvesting often cannot meet the demands for large‐scale restoration and may also result in depletion of native seed resources through over harvesting. To improve seed production and decrease seed costs, seed production systems have been established in several countries to generate native seeds based on agricultural or horticultural production methods or by managing natural populations. However, there is a need to expand these production systems which have a primary focus on herbaceous species to also include slower maturing shrub and tree seed. Here we propose that to reduce the threat of overharvest on the viability of natural populations, seed collection from natural populations should be replaced or supplemented by seed production systems. This overview of seed production systems demonstrates how to maximize production and minimize unintended selection bias so that native seed batches maintain genetic diversity and adaptability to underpin the success of ecological restoration programs.     

Hypertonic sodium chloride and mannitol induces COX-2 via different signaling pathways in mouse cortical collecting duct M-1 cells

The kidney cortical collecting duct is an important site for the maintenance of sodium balance. Previous studies have shown that, in renal medullary cells, hypertonic stress induces expression of cyclooxygenase-2 (COX-2) via NF-kappaB activation, but little is known about COX-2 expression in response to hypertonicity in the cortical collecting duct. Therefore, we examined the mechanism of hypertonic induction of COX-2 in M-1 cells derived from mouse cortical collecting duct. Induction of COX-2 protein was detected within 6 h of treatment with hypertonic sodium chloride. The treatment also increased COX-2 mRNA accumulation in a cycloheximide-independent manner, suggesting that ongoing protein synthesis is not required for COX-2 induction. Using reporter plasmids containing 0.2-, 0.3-, and 1.5-kb fragments of the COX-2 promoter, we found that hypertonic induction of COX-2 was due to an increase in promoter activity. The COX-2-inductive effect of hypertonicity was inhibited by SB203580, indicating that the effect is mediated by p38 MAPK. Since p38 MAPK can activate NF-kappaB, we made point mutations in the NF-kappaB binding site within the COX-2 promoter. The mutations did not block the induction of COX-2 promoter activity by hypertonic sodium chloride, and hypertonic sodium chloride failed to activate NF-kappaB binding site-driven reporter gene constructs. In contrast, hypertonic mannitol activated NF-kappaB, indicating that hypertonic mannitol and hypertonic sodium chloride activate COX-2 by different mechanisms. Thus, induction of COX-2 expression in M-1 cells by hypertonic sodium chloride does not involve activation of NF-kappaB. Furthermore, the signal transduction pathways that respond to hypertonic stress vary for different osmolytes in cortical collecting duct cells.     

梅花和蜡梅被西方世界认识的历史考证

文中收集、整理了自17世纪至20世纪共22位西方学者(包括西方化的日本学者)认识、命名、收集、培育梅花Prunus mume、蜡梅Chimonanthus praecox的文献资料及其历史贡献,并比较两种植物被西方世界认识的历史差异,分析成因。最后探讨梅花、蜡梅在世界范围内推广的困境及原因。     

Blühverhalten und Bestäubungsbiologie vonCarludovica palmata (Cyclanthaceae) — ein ökologisches Paradoxon

In natural habitats ofCarludovica palmata (Colombia) numerous stingless bees (Meliponinae) were observed as pollen collectors at the inflorescences and were believed to be the pollinators of the entomophilous flowers. Male flowers surround female ones on the spadix in a regular fashion. At first, the filiform, strongly scented staminodia of the female flowers unfold. After they have dropped, the anthers open, and finally the male flowers fall to ground. Only then, the stigmata of the female flowers are exposed. Previous literature references on proterogyny and the drying-up of the stigmata prior to the male phase inCarludovica are in conflict with these observations on the course of anthesis and pollination.

     

Pollination mechanisms in sixCampanula species (Campanulaceae)

Pollination mechanisms within the genusCampanula were studied. Tests were undertaken to examine whether in vitro culture of pollen grains can serve as a useful tool for understanding the self- and cross-pollination mechanisms among species. Characteristics of pollen germination were interpreted in relation to mating system and floral biology. Four annual species (Campanula kremeri, C. dichotoma, C. afra, C. lusitanica), and two perennial species (C. rotundifolia andC. persicifolia) were investigated. In the annual species pollen germinability is controlled by (1) the age of pollen and/or (2) in what position pollen is deposited around the style. Correlations were found between pollen germinability and mating system in three of the four annual species. No correlations were found either between germinability and age of pollen or position on the style in the perennial species. Pollen germinability reached its maximum in the middle of the male phase in all species, except forC. dichotoma, which had a decreasing germinability throughout anthesis. The germinability was lowest at the time of stigma receptivity for all species except forC. persicifolia, where the stigma did not develop as long as pollen remained on the style. The pollen collecting hairs and pollen removal have been found to play an important role controlling the stigma development, thus affecting self-pollination. This was especially pronounced inC. persicifolia. Further studies will be undertaken to elucidate factors influencing pollination within the genusCampanula.     

Arachidonic acid inhibits epithelial Na channel via cytochrome P450 (CYP) epoxygenase-dependent metabolic pathways

We used the patch-clamp technique to study the effect of arachidonic acid (AA) on epithelial Na channels (ENaC) in the rat cortical collecting duct (CCD). Application of 10 microM AA decreased the ENaC activity defined by NPo from 1.0 to 0.1. The dose-response curve of the AA effect on ENaC shows that 2 microM AA inhibited the ENaC activity by 50%. The effect of AA on ENaC is specific because neither 5,8,11,14-eicosatetraynoic acid (ETYA), a nonmetabolized analogue of AA, nor 11,14,17-eicosatrienoic acid mimicked the inhibitory effect of AA on ENaC. Moreover, inhibition of either cyclooxygenase (COX) with indomethacin or cytochrome P450 (CYP) omega-hydroxylation with N-methylsulfonyl-12,12-dibromododec-11-enamide (DDMS) failed to abolish the effect of AA on ENaC. In contrast, the inhibitory effect of AA on ENaC was absent in the presence of N-methylsulfonyl-6-(propargyloxyphenyl)hexanamide (MS-PPOH), an agent that inhibits CYP-epoxygenase activity. The notion that the inhibitory effect of AA is mediated by CYP-epoxygenase-dependent metabolites is also supported by the observation that application of 200 nM 11,12-epoxyeicosatrienoic acid (EET) inhibited ENaC in the CCD. In contrast, addition of 5,6-, 8,9-, or 14,15-EET failed to decrease ENaC activity. Also, application of 11,12-EET can still reduce ENaC activity in the presence of MS-PPOH, suggesting that 11,12-EET is a mediator for the AA-induced inhibition of ENaC. Furthermore, gas chromatography mass spectrometry analysis detected the presence of 11,12-EET in the CCD and CYP2C23 is expressed in the principal cells of the CCD. We conclude that AA inhibits ENaC activity in the CCD and that the effect of AA is mediated by a CYP-epoxygenase-dependent metabolite, 11,12-EET.     

Ameboid cells in spermatogenic cysts of caecilian testis

Sertoli cells constitute a permanent feature of the testis lobules in caecilians irrespective of the functional state of the testis. The developing germ cells are intimately associated with the Sertoli cells, which are adherent to the basal lamina, until spermiation. There are irregularly shaped cells in the cores of the testis lobules that interact with germ cells at the face opposite to their attachment with Sertoli cells. These irregularly shaped (ameboid) cells first appear in the lumen of the cysts containing primary spermatocytes and are continually present until spermiation. We did not observe any cytoplasmic continuity between a Sertoli cell and an ameboid cell. Both light microscopic and TEM observations reveal a phagocytic role for the ameboid cells: they scavenge the residual bodies shed by spermatozoa. Organization of the ameboid cells is grossly different from that of the spermatogenic and Sertoli cells. They appear to develop from the epithelium at the juncture of the collecting ductule with the testis lobule.     

Nitric Oxide Links the Apical Na+ Transport to the Basolateral K+ Conductance in the Rat Cortical Collecting Duct

We have used the patch clamp technique to study the effects of inhibiting the apical Na⁺ transport on the basolateral small-conductance K⁺ channel (SK) in cell-attached patches in cortical collecting duct (CCD) of the rat kidney. Application of 50 μM amiloride decreased the activity of SK, defined as nP _o (a product of channel open probability and channel number), to 61% of the control value. Application of 1 μM benzamil, a specific Na⁺ channel blocker, mimicked the effects of amiloride and decreased the activity of the SK to 62% of the control value. In addition, benzamil reduced intracellular Na⁺ concentration from 15 to 11 mM. The effect of amiloride was not the result of a decrease in intracellular pH, since addition 50 μM 5-(n-ethyl-n-isopropyl) amiloride (EIPA), an agent that specifically blocks the Na/H exchanger, did not alter the channel activity. The inhibitory effect of amiloride depends on extracellular Ca²⁺ because removal of Ca²⁺ from the bath abolished the effect. Using Fura-2 AM to measure the intracellular Ca²⁺, we observed that amiloride and benzamil significantly decreased intracellular Ca²⁺ in the Ca²⁺-containing solution but had no effect in a Ca²⁺-free bath. Furthermore, raising intracellular Ca²⁺ from 10 to 50 and 100 nM with ionomycin increased the activity of the SK in cell-attached patches but not in excised patches, suggesting that changes in intracellular Ca²⁺ are responsible for the effects on SK activity of inhibition of the Na⁺ transport. Since the neuronal form of nitric oxide synthase (nNOS) is expressed in the CCD and the function of the nNOS is Ca²⁺ dependent, we examined whether the effects of amiloride or benzamil were mediated by the NO-cGMP–dependent pathways. Addition of 10 μM S-nitroso-n-acetyl-penicillamine (SNAP) or 100 μM 8-bromoguanosine 3′:5′-cyclic monophosphate (8Br-cGMP) completely restored channel activity when it had been decreased by either amiloride or benzamil. Finally, addition of SNAP caused a significant increase in channel activity in the Ca²⁺-free bath solution. We conclude that Ca²⁺-dependent NO generation mediates the effect of inhibiting the apical Na⁺ transport on the basolateral SK in the rat CCD.