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51.
52.
Pushing, pulling and trapping--modes of motor protein supported protein translocation 总被引:1,自引:0,他引:1
Protein translocation across the cellular membranes is an ubiquitous and crucial activity of cells. This process is mediated by translocases that consist of a protein conducting channel and an associated motor protein. Motor proteins interact with protein substrates and utilize the free energy of ATP binding and hydrolysis for protein unfolding, translocation and unbinding. Since motor proteins are found either at the cis- or trans-side of the membrane, different mechanisms for translocation have been proposed. In the Power stroke model, cis-acting motors are thought to push, while trans-motors pull on the substrate protein during translocation. In the Brownian ratchet model, translocation occurs by diffusion of the unfolded polypeptide through the translocation pore while directionality is achieved by trapping and refolding. Recent insights in the structure and function of the molecular motors suggest that different mechanisms can be employed simultaneously. 相似文献
53.
森林生态系统土壤CO2释放随海拔梯度的变化及其影响因子 总被引:2,自引:0,他引:2
联合国气候框架公约的签署提升了人们对全球变暖、碳循环的关注。土壤CO2释放作为土壤-大气CO2交换的主要途径之一,成为了各国生态学家研究的重点内容。通过对1800~2155m海拔梯度上森林生态系统土壤CO2释放进行研究,揭示了较小空间尺度上土壤CO2释放的变化规律及其控制机制。在研究区域内,随着海拔梯度的增加,森林土壤CO2释放由(1.94±006) μmol m-2 s-1逐渐增加至(2.22±0.07) μ mol m-2 s-1。土壤温度、土壤水分、土壤有机碳(SOC)、全N、全P与土壤CO2释放呈显著正相关(n=14, P<0.05);土壤容重与土壤CO2释放速率呈显著负相关(n=14,P<0.05);土壤pH对土壤CO2释放影响不显著。作为一个复杂的生态学过程,环境因子及其交互作用对土壤CO2释放产生影响,为了减少因子共线性影响,逐步降低因子维数,采用主成分分析(PCA)揭示了土壤温度、土壤水分、SOC、全N、全P、容重6个因子的联合作用,其累积贡献率达到了57%以上;进一步运用逐步回归分析方法,探讨了影响土壤CO2释放沿海拔梯度分布的主导因子,结果表明土壤水分是研究区域森林生态系统土壤CO2释放沿海拔梯度变化的主导因子。 相似文献
54.
Spores of Nosema bombycis derived from diseased insects were highly purified by Urografin density gradient centrifugation. Antheraea eucalypti cells were inoculated with the purified spores primed with 0.1 n KOH solution to start a continuous propagation of N. bombycis in cell culture. The first increase in the number of infected A. eucalypti cells was observed at 48 hr postinoculation, and it was caused by the secondary infective forms of N. bombycis. The secondary infective forms were produced during the course of sporoblast differentiation. The parasites in cell cultures divided synchronously until 36 hr postinoculation. Mature spores were observed initially 6 days postinoculation at 27°C. The infected cultures were subcultured extensively for more than 1 year with the addition of healthy A. eucalypti cells. 相似文献
55.
Faunal composition of geometrid moths changes with altitude in an Andean montane rain forest 总被引:2,自引:0,他引:2
Aim The objective of this study was to describe and interpret the changes in faunal composition in the moth family Geometridae (Lepidoptera) along a small‐scale elevational gradient in a tropical montane rain forest. This gradient was compared with a large‐scale latitudinal gradient in Europe. Location Investigations were carried out in the province Zamora‐Chinchipe in southern Ecuador along a gradient ranging from 1040 to 2677 m above sea level at twenty‐two sites. Methods Moths were sampled with light‐traps in three field periods in 1999 and 2000 and subsequently sorted and determined to species or morphospecies. Results We analysed 13,938 specimens representing 1010 species of geometrid moths. The proportional contribution of subtaxa to the local geometrid fauna changes along the elevational gradient at all systematic levels considered. While proportions of species of the subfamilies Ennominae, Sterrhinae and Geometrinae significantly decrease, the proportion of Larentiinae increases with increasing altitude. Changes also occur within the subfamilies Ennominae and Larentiinae. The host–plant specialist ennomine tribes Cassymini, Macariini and Palyadini completely vanish, and the proportion of the tribe Boarmiini decreases at high altitudes. In contrast, the remaining tribes (mostly comprising polyphagous species) either do not show proportional changes (Azelinini, Nacophorini, Nephodiini, Ourapterygini) or even increase (Caberini, ‘Cratoptera group’). Within Larentiinae, the species proportion of the genus Eois decreases, whereas concomitantly the proportion of Eupithecia increases. There is a remarkable similarity between the altitudinal patterns in Ecuador and those found along the latitudinal gradient in Europe. Main conclusions Species of the subfamily Larentiinae seem to be particularly well‐adapted to harsh environmental conditions, towards both high altitudes and latitudes. They might disproportionately profit from lower predation at higher altitudes. Many changes in the faunal composition can be explained by expected host–plant requirements of the species involved. Our results show that diversity estimates based on taxon ratios which are assumed to be constant must be regarded with caution because such ratios can change rapidly along environmental gradients. 相似文献
56.
What determines the vertical distribution of phytoplankton in different aquatic environments remains an open question. To address this question, we develop a model to explore how phytoplankton respond through growth and movement to opposing resource gradients and different mixing conditions. We assume stratification creates a well-mixed surface layer on top of a poorly mixed deep layer and nutrients are supplied from multiple depth-dependent sources. Intraspecific competition leads to a unique strategic equilibrium for phytoplankton, which allows us to classify the distinct vertical distributions that can exist. Biomass can occur as a benthic layer (BL), a deep chlorophyll maximum (DCM), or in the mixed layer (ML), or as a combination of BL+ML or DCM+ML. The ML biomass can be limited by nutrients, light, or both. We predict how the vertical distribution, relative resource limitation, and biomass of phytoplankton will change across environmental gradients. We parameterized our model to represent potentially light and phosphorus limited freshwater lakes, but the model is applicable to a broad range of vertically stratified systems. Increasing nutrient input from the sediments or to the mixed layer increases light limitation, shifts phytoplankton towards the surface, and increases total biomass. Increasing background light attenuation increases light limitation, shifts the phytoplankton towards the surface, and generally decreases total biomass. Increasing mixed layer depth increases, decreases, or has no effect on light limitation and total biomass. Our model is able to replicate the diverse vertical distributions observed in nature and explain what underlying mechanisms drive these distributions. 相似文献
57.
Northeast China Transect (NECT), one of the fifteen International Biosphere-Geosphere Programme (IGBP) terrestrial transects, has been established for 10 years by Prof. Zhang Xin-Shi, through a core project of the IGBP - the Global Change and Terrestrial Ecosystems (GCTE). This transect is located in the mid-latitude semi-arid region, ranging 42-46°N latitude and 110-132癊 longitude. The primary driving force for global change is precipitation and the secondary one is land use intensity. Research progresses have been performed during the past decade in the following aspects: ecological database development, climate and its variability, ecophysiological response of plants to environments, vegetation and landscape changes, biodiversity patterns and their changes, plant functional types and traits with relation to climatic gradient, productivity and carbon dynamics, pollen-vegetation relationship, trace gas emissions, land use and land cover changes, as well as biogeographical and biogeochemical modelling. In order to achieve the higher level of integrated research, the NECT needs the consistent basic data sets within the same framework, further field experiments and observations, integrated simulations of vegetation structure, process and function from patch, landscape to biome scales, intercomparisons of results and simulations within the transect and to other IGBP transects, multidisciplinary research, national and international co-ordinates, and full scientific plan and implementation strategy. 相似文献
58.
Litterfall Production Along Successional and Altitudinal Gradients of Subtropical Monsoon Evergreen Broadleaved Forests in Guangdong, China 总被引:3,自引:0,他引:3
Guoyi Zhou Lili Guan Xiaohua Wei Deqiang Zhang Qianmei Zhang Junhua Yan Dazhi Wen Juxiu Liu Shuguang Liu Zhongliang Huang Guohui Kong Jiangming Mo Qingfa Yu 《Plant Ecology》2007,188(1):77-89
Evaluation of litterfall production is important for understanding nutrient cycling, forest growth, successional pathways, and interactions with environmental variables in forest ecosystems. Litterfall was intensively studied during the period of 1982–2001 in two subtropical monsoon vegetation gradients in the Dinghushan Biosphere Reserve, Guangdong Province, China. The two gradients include: (1) a successional gradient composed of pine forest (PF), mixed pine and broadleaved forest (MF) and monsoon evergreen broadleaved forest (BF), and (2) an altitudinal gradient composed of Baiyunci ravine rain forest (BRF), Qingyunci ravine rain forest (QRF), BF and mountainous evergreen broadleaved forest (MMF). Mean annual litterfall production was 356, 861 and 849 g m−2 for PF, MF and BF of the successional gradient, and 1016, 1061, 849 and 489 g m−2 for BRF, QRF, BF and MMF of the altitudinal gradient, respectively. As expected, mean annual litterfall of the pioneer forest PF was the lowest, but rapidly increased over the observation period while those in other forests were relatively stable, confirming that forest litterfall production is closely related to successional stages and growth patterns. Leaf proportions of total litterfall in PF, MF, BF, BRF, QRF and MMF were 76.4%, 68.4%, 56.8%, 55.7%, 57.6% and 69.2%, respectively, which were consistent with the results from studies in other evergreen broadleaved forests. Our analysis on litterfall monthly distributions indicated that litterfall production was much higher during the period of April to September compared to other months for all studied forest types. Although there were significant impacts of some climate variables (maximum and effective temperatures) on litterfall production in some of the studied forests, the mechanisms of how climate factors (temperature and rainfall) interactively affect litterfall await further study. 相似文献
59.
Tatsuya Amano Robert P. Freckleton Simon A. Queenborough Simon W. Doxford Richard J. Smithers Tim H. Sparks William J. Sutherland 《Proceedings. Biological sciences / The Royal Society》2014,281(1779)
To generate realistic projections of species’ responses to climate change, we need to understand the factors that limit their ability to respond. Although climatic niche conservatism, the maintenance of a species’s climatic niche over time, is a critical assumption in niche-based species distribution models, little is known about how universal it is and how it operates. In particular, few studies have tested the role of climatic niche conservatism via phenological changes in explaining the reported wide variance in the extent of range shifts among species. Using historical records of the phenology and spatial distribution of British plants under a warming climate, we revealed that: (i) perennial species, as well as those with weaker or lagged phenological responses to temperature, experienced a greater increase in temperature during flowering (i.e. failed to maintain climatic niche via phenological changes); (ii) species that failed to maintain climatic niche via phenological changes showed greater northward range shifts; and (iii) there was a complementary relationship between the levels of climatic niche conservatism via phenological changes and range shifts. These results indicate that even species with high climatic niche conservatism might not show range shifts as instead they track warming temperatures during flowering by advancing their phenology. 相似文献
60.
生态脆弱带内部空间分异结构与脆弱度划分 总被引:6,自引:0,他引:6
内蒙乌盟后山及河北坝上地区是研究土地利用的人为驱动,气候驱动同全球变化关系的理想剖面。区内气候风蚀力代表了自然地理条件的主分异方向,其值在70-3之间,高低差别很大,区内42a内单位面积上牲畜头数累积减少量以旱作界线附近为最高,向南北两侧减低,且南部高于北部。20世纪80年代沙漠化的发展并没改变本世纪70年代沙漠化程度分布的基本格局。两介时代具有相同的空间分布规律;沿旱作农业北界附近的旗县为沙漠化最严重的地带,向南部坝上农业地带和北部牧业地带递减。虽然社会经济因素仍以西北东南为分异的主方向,但滥垦加剧了农牧交错带梯度分异,旱作界线附近构成了社会经济因子分异的跃变地带。对区内20个旗县13个因子的旋转主成分分析表明,前4个主成分约分别为33%,21%,15%和14%,各自代表社会经济条件,灾害,气候风蚀力和沙尘天气四类要素。根据其分异规律,自东南向西北可将本区划为南部农牧中度脆弱,中部牧农重度脆弱和北部牧业轻度脆弱3个弧形地带。 相似文献