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981.
MarcOlivier MartinGuay Michaël Belluau Benoit Ct Ira Tanya Handa Mark D. Jewell Rim Khlifa Alison D. Munson Maxime Rivest Joann K. Whalen David Rivest 《Ecology and evolution》2022,12(1)
- Soil C is the largest C pool in forest ecosystems that contributes to C sequestration and mitigates climate change. Tree diversity enhances forest productivity, so diversifying the tree species composition, notably in managed forests, could increase the quantity of organic matter being transferred to soils and alter other soil properties relevant to the C cycle.
- A ten‐year‐old tree diversity experiment was used to study the effects of tree identity and diversity (functional and taxonomic) on soils. Surface (0–10 cm) mineral soil was repeatedly measured for soil C concentration, C:N ratio, pH, moisture, and temperature in twenty‐four tree species mixtures and twelve corresponding monocultures (replicated in four blocks).
- Soil pH, moisture, and temperature responded to tree diversity and identity. Greater productivity in above‐ and below‐ground tree components did not increase soil C concentration. Soil pH increased and soil moisture decreased with functional diversity, more specifically, when species had different growth strategies and shade tolerances. Functional identity affected soil moisture and temperature, such that tree communities with more slow‐growing and shade‐tolerant species had greater soil moisture and temperature. Higher temperature was measured in communities with broadleaf‐deciduous species compared to communities with coniferous‐evergreen species.
- We conclude that long‐term soil C cycling in forest plantations will likely respond to changes in soil pH, moisture, and temperature that is mediated by tree species composition, since tree species affect these soil properties through their litter quality, water uptake, and physical control of soil microclimates.
982.
夜蛾黑卵蜂是草地贪夜蛾卵期的一种重要寄生性天敌,适宜的寄主是实现其规模化繁育及应用的基础。本文应用实验种群生命表方法,分析、评价了草地贪夜蛾、斜纹夜蛾及甜菜夜蛾这3种夜蛾卵上夜蛾黑卵蜂的生长发育及种群增长潜能。结果表明,夜蛾黑卵蜂在3种夜蛾卵上均能顺利完成幼期发育且出蜂日期较为整齐,羽化当日即为产卵高峰日,羽化后3 d内的寄生卵量占总产卵量的80%以上。子代羽化率介于77.90%~85.56%之间,雌性比率介于0.53~0.59之间。不同寄主繁育的夜蛾黑卵蜂雄蜂后足胫节长度无明显差异,但以甜菜夜蛾卵繁育的雌蜂的后足胫节最长(152.00μm),斜纹夜蛾卵繁育的次之(146.33μm),草地贪夜蛾卵繁育的最短(142.67μm)。以甜菜夜蛾卵繁育的夜蛾黑卵蜂的内禀增长率(rm)、净生殖力(R0)及周限增长率(λ)均最高,分别为0.3486、57.94和1.4171,以斜纹夜蛾卵繁育的次之(rm,0.3091;R0,49.60;λ,1.3623),而以草地贪夜蛾卵繁育的最小(rm,... 相似文献
983.
Thomas Weisse 《International Review of Hydrobiology》1991,76(3):327-337
The relative contribution to the pelagic carbon flux of bacteria, autotrophic picoplankton (APP), and heterotrophic nano- and microplankton is highest in pristine oligotrophic waters. Increased nutrient loading stimulates picoplankton production but reduces the importance of the microbial food web. Not all microorganisms are affected in the same way by eutrophication and contaminant enrichment. In contrast to bacteria and heterotrophic nanoflagellates, the APP was found to be highly sensitive to heavy metal contamination. The changing significance of the microbial loop has far-reaching consequences for the entire ecosystem including alterations occurring in the benthos. The structure and functioning of the microbial food web is similar in marine, brackish, and freshwater ecosystems. 相似文献
984.
A cell culture platform for quantifying metabolic substrate oxidation in bicarbonate-buffered medium
James R. Krycer Mary Lor Rebecca L. Fitzsimmons James E. Hudson 《The Journal of biological chemistry》2022,298(2)
Complex diseases such as cancer and diabetes are underpinned by changes in metabolism, specifically by which and how nutrients are catabolized. Substrate utilization can be directly examined by measuring a metabolic endpoint rather than an intermediate (such as a metabolite in the tricarboxylic acid cycle). For instance, oxidation of specific substrates can be measured in vitro by incubation of live cultures with substrates containing radiolabeled carbon and measuring radiolabeled carbon dioxide. To increase throughput, we previously developed a miniaturized platform to measure substrate oxidation of both adherent and suspension cells using multiwell plates rather than flasks. This enabled multiple conditions to be examined simultaneously, ideal for drug screens and mechanistic studies. However, like many metabolic assays, this was not compatible with bicarbonate-buffered media, which is susceptible to alkalinization upon exposure to gas containing little carbon dioxide such as air. While other buffers such as HEPES can overcome this problem, bicarbonate has additional biological roles as a metabolic substrate and in modulating hormone signaling. Here, we create a bicarbonate-buffered well-plate platform to measure substrate oxidation. This was achieved by introducing a sealed environment within each well that was equilibrated with carbon dioxide, enabling bicarbonate buffering. As proof of principle, we assessed metabolic flux in cultured adipocytes, demonstrating that bicarbonate-buffered medium increased lipogenesis, glucose oxidation, and sensitivity to insulin in comparison to HEPES-buffered medium. This convenient and high-throughput method facilitates the study and screening of metabolic activity under more physiological conditions to aid biomedical research. 相似文献
985.
人类活动造成大气二氧化碳(CO2)浓度不断升高,使当今世界面临着气候变化的重大危机。微生物CO2固定为实现地球“碳中和”提供了一条有前景的绿色发展路线。与自养微生物相比,异养微生物具有更快的生长速度和更先进的遗传工具,但是其固定CO2的能力还很有限。近年来,基于合成生物学技术强化异养微生物CO2固定受到诸多关注,主要包括优化能量供给、改造羧化途径以及基于异养微生物间接固定CO2。本综述将围绕上述3个方面重点讨论异养微生物CO2固定的研究进展,为将来更好地利用微生物CO2固定技术实现“碳达峰、碳中和”提供参考。 相似文献
986.
皖南休宁县蓝田地区震旦系皮园村组和下寒武统荷塘组主要由硅质岩和黑色页岩组成。皮园村组硅质岩的分析结果表明:δ13Corg分析值落在-36.39‰—-31.45‰范围之内,而且从该剖面的底部到顶部呈现出逐步下降的趋势,可能归之于大气和海洋中O2/CO2比值上升,生物氧化作用增强所致;有机碳(TOC)含量<1%,有机氮(TON)含量<1‰,两者均处于蓝田地区埃迪卡拉系—下寒武统地层的最低值,证明晚震旦世时期该地区海洋生物产量比前期(蓝田期)海洋产量显著下降,可能归之于海水加深,不利于海洋生物的繁殖。荷塘组黑色页岩的δ13Corg分析值落在-33‰—-31‰范围之内,TOC和TON含量与皮园村组比较有大幅度提高,尤其黑色页岩下段TOC含量达到13.85%,证明早寒武世海洋生物得到了大发展。由于新元古代冰期后海洋藻类的大量涌现和早寒武世海洋生物得到大发展,生物光合作用产生大量的O2,导致大气和海水中O2/CO2比值进一步升高,可能是引发“寒武纪生物大爆发”的重要因素。 相似文献
987.
Yi-Chin Candace Tsai Lynette Liew Zhijun Guo Di Liu Oliver Mueller-Cajar 《The Journal of biological chemistry》2022,298(1)
The CO2-fixing enzyme rubisco is responsible for almost all carbon fixation. This process frequently requires rubisco activase (Rca) machinery, which couples ATP hydrolysis to the removal of inhibitory sugar phosphates, including the rubisco substrate ribulose 1,5-bisphosphate (RuBP). Rubisco is sometimes compartmentalized in carboxysomes, bacterial microcompartments that enable a carbon dioxide concentrating mechanism (CCM). Characterized carboxysomal rubiscos, however, are not prone to inhibition, and often no activase machinery is associated with these enzymes. Here, we characterize two carboxysomal rubiscos of the form IAC clade that are associated with CbbQO-type Rcas. These enzymes release RuBP at a much lower rate than the canonical carboxysomal rubisco from Synechococcus PCC6301. We found that CbbQO-type Rcas encoded in carboxysome gene clusters can remove RuBP and the tight-binding transition state analog carboxy-arabinitol 1,5-bisphosphate from cognate rubiscos. The Acidithiobacillus ferrooxidans genome encodes two form IA rubiscos associated with two sets of cbbQ and cbbO genes. We show that the two CbbQO activase systems display specificity for the rubisco enzyme encoded in the same gene cluster, and this property can be switched by substituting the C-terminal three residues of the large subunit. Our findings indicate that the kinetic and inhibitory properties of proteobacterial form IA rubiscos are diverse and predict that Rcas may be necessary for some α-carboxysomal CCMs. These findings will have implications for efforts aiming to introduce biophysical CCMs into plants and other hosts for improvement of carbon fixation of crops. 相似文献
988.
Danielle K. Walkup Anna Michelle Lawing Toby J. Hibbitts Wade A. Ryberg 《Ecology and evolution》2022,12(2)
The western massasauga (Sistrurus tergeminus) is a small pit viper with an extensive geographic range, yet observations of this species are relatively rare. They persist in patchy and isolated populations, threatened by habitat destruction and fragmentation, mortality from vehicle collisions, and deliberate extermination. Changing climates may pose an additional stressor on the survival of isolated populations. Here, we evaluate historic, modern, and future geographic projections of suitable climate for S. tergeminus to outline shifts in their potential geographic distribution and inform current and future management. We used maximum entropy modeling to build multiple models of the potential geographic distribution of S. tergeminus. We evaluated the influence of five key decisions made during the modeling process on the resulting geographic projections of the potential distribution, allowing us to identify areas of model robustness and uncertainty. We evaluated models with the area under the receiver operating curve and true skill statistic. We retained 16 models to project both in the past and future multiple general circulation models. At the last glacial maximum, the potential geographic distribution associated with S. tergeminus occurrences had a stronghold in the southern part of its current range and extended further south into Mexico, but by the mid‐Holocene, its modeled potential distribution was similar to its present‐day potential distribution. Under future model projections, the potential distribution of S. tergeminus moves north, with the strongest northward trends predicted under a climate scenario increase of 8.5 W/m2. Some southern populations of S. tergeminus have likely already been extirpated and will continue to be threatened by shifting availability of suitable climate, as they are already under threat from desertification of grasslands. Land use and habitat loss at the northern edge of the species range are likely to make it challenging for this species to track suitable climates northward over time. 相似文献
989.
990.