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101.
M. L. Champigny 《Photosynthesis research》1985,6(3):273-286
In green leaves and a number of algae, photosynthetically derived carbon is ultimately converted into two carbohydrate end-products, sucrose and starch. Drainage of carbon from the Calvin cycle proceeds via triose phosphate, fructose 6-phosphate and glycollate. Gluconeogenesis in photosynthetic cells is controlled by light, inorganic phosphate and phosphorylated sugars. Light stimulates the production of dihydroxyacetone phosphate, the initial substrate for sucrose and starch synthesis, and inhibits the degradative pathways in the chloroplast. Phosphate inactivates reactions of synthesis and activates reactions of degradation. Among the phosphorylated sugars a special role is allocated to fructose 2,6-bisphosphate, which is present in the cytoplasm at very low concentrations and inhibits sucrose synthesis directly by inactivating pyrophosphatedependent phosphofructokinase. The synthesis of sucrose plays a central role in the partitioning of photosynthetic carbon. The cytoplasmic enzymes, fructose bisphosphate phosphatase and sucrose phosphate synthase are likely key points of regulation. The regulation is carried out by several effector metabolites. Fructose 2,6-bisphosphate is likely to be the main coordinator of the rate of sucrose synthesis, hence of photosynthetic carbon partitioning between sucrose and starch.Paper presented at the FESP meeting (Strasbourg, 1984) 相似文献
102.
Spectral changes in fluorescence of phycobilisomes (PBS) of A. variabilis treated with proteinase K were studied at room and liquid nitrogen temperature. In control PBS, the relative yield of 77 K fluorescence of F686 was very high, and those of F655 and F666 were low. In PBS treated with proteinase K for less than 1 h, F686 decreased, and F655 and F666 increased. In PBS treated with proteinase K for 2 h, F655 was the main peak of fluorescence emission, F686 was the second peak, the fluorescence emission peak of F666 disappeared. In PBS treated with proteinase K for more than 8 h, F655 showed only one fluorescence emission peak.We suggested that phycobiliporteins in the PBS of A. variabilis constitute an energy transfer chain, shown as follows:{fx91-1}The linkages between APC and APC-B, C-PC and APC, and C-PC and APC-B had different sensitivity towards proteinase K. 相似文献
103.
Leaf photosynthesis rate of the C4 species Paspalum plicatulum Michx was virtually CO2-saturated at normal atmospheric CO2 concentration but transpiration decreased as CO2 was increased above normal concentrations thereby increasing transpiration efficiency. To test whether this leaf response led growth to be CO2-sensitive when water supply was restricted, plants were grown in sealed pots of soil as miniature swards. Water was supplied either daily to maintain a constant water table, or at three growth restricting levels on a 5-day drying cycle. Plants were either in a cabinet with normal air (340 mol (CO2) mol-1 (air)) or with 250 mol mol-1 enrichment. Harvesting was by several cycles of defoliation.With abundant water supply high CO2 concentration did not cause increased growth, but it did not cause an increase in growth over a wide range of growth-limiting water supplies either. Only when water supply was less than 30–50% of the amount used by the stand with a water-table was there evidence that dry weight growth was enhanced by high CO2. In addition, with successive regrowth, the enhancing effect under a regime of minimal water allocations, became attenuated. Examination of leaf gas exchange, growth and water use data showed that in the long term stomatal conductance responses were of little significance in matching plant water use to low water allocation; regulation of leaf area was the mechanism through which consumption matched supply. Since high CO2 effects operate principally via stomatal conductance in C4 species, we postulate that for this species higher CO2 concentrations expected globally in future will not have much effect on long term growth. 相似文献
104.
R. J. HELDER 《Plant, cell & environment》1985,8(6):399-408
Abstract The quantitative approach used here is based on a model comprising a well-stirred medium, an unstirred layer, and a CO2 absorbing leaf. The unstirred layer is divided up by planes into a number of sub-layers. Within each plane the concentration of each solute is everywhere the same as is the electric potential. These variables constitute the basic data. Thus the planes were characterized by their pH value. An equation is derived which enables the calculation of the basic data of a plane from the known data of another plane. In this way it is possible to calculate the basic data for all planes. From these data the rate of assimilation, the thickness of the unstirred layer and its sub-layers, the fluxes across the sub-layers and the conversions among the carbon components can be estimated. The CO2 flux decreases, and the HCO?3 flux increases towards the leaf. There are negative fluxes of OH& and CO2–3. H+ fluxes are of minor importance and can be ignored if the pH of the medium is higher than 8.0, provided no non-inorganic C buffers with appropriate pKa are present. The significance of the carbon diffusion facilitating effect of an inorganic carbon system is expressed in various ways. The values obtained represent maxima, as the assumption is made that the equilibrium reactions are very fast. It is argued that even better effects are possible if the back-diffusion of CO2–3 could be prevented by lowering the pH of the unstirred layer. 相似文献
105.
The nitrogen balance of Raphanus sativus X raphanistrum plants. I. Daily nitrogen use under high nitrate supply 总被引:1,自引:1,他引:0
E.-D. SCHULZE † G. KOCH F. PERCIVAL H. A. MOONEY C. CHU 《Plant, cell & environment》1985,8(9):713-720
Abstract Growth-chamber cultivated Raphanus plants accumulate nitrate during their vegetative growth. After 25 days of growth at a constant supply to the roots of 1 mol m?3 (NO?3) in a balanced nutrient solution, the oldest leaves (eight-leaf stage) accumulated 2.5% NO?3-nitrogen (NO3-N) in their lamina, and almost 5% NO3-N in their petioles on a dry weight basis. This is equivalent to approximately 190 and 400 mol?3 m?3 concentration of NO?3 in the lamina and the petiole, respectively, as calculated on a total tissue water content basis. Measurements were made of root NO?3 uptake, NO?3 fluxes in the xylem, nitrate uptake by the mesophyll cells, and nitrate reduction as measured by an in vivo test. NO?3 uptake by roots and mesophyll cells was greater in the light than in the dark. The NO?3 concentration in the xylem fluid was constant with leaf age, but showed a distinct daily variation as a result of the independent fluxes of root uptake, transpiration and mesophyll uptake. NO?3 was reduced in the leaf at a higher rate in the light than in the dark. The reduction was inhibited at the high concentrations calculated to exist in the mesophyll vacuoles, but reduction continued at a low rate, even when there was no supply from the incubation medium. Sixty-four per cent of the NO?3 influx was turned into organic nitrogen, with the remaining NO?3 accumulating in both the light and the dark. 相似文献
106.
Abstract Spore-forming sulfate-reducing bacteria (SRB) were enriched selectively from various kinds of aerobic soils with fatty acids as the sole carbon and energy source. A Gram-negative motile rod-shaped bacterium, which produced gas vacuoles during sporulation was isolated. It degraded alcohols, aromatic and n-fatty acids (up to C18 ) except for propionate, completely to CO2 . Sulfate, sulfite, thiosulfate or elemental sulfur served as electron acceptors. Because of its sensitivity to H2 S, the isolate never produced more than 8 mM dissolved sulfide at pH 7.0. G + C-content of the DNA was 48.0 mol %. The isolated strain Pato is described as a new species Desulfotomaculum sapomandens . 相似文献
107.
Cytochrome c oxidase preparations from bovine heart muscle contain 1 zinc per 2 irons. Metal contents of nine preparations determined by inductively coupled plasma atomic emission spectrometry (ICP-AES) show that Cu, Fe and Zn are the only metals present in significant amounts with average Cu/Fe, Fe/Zn, and Cu/Zn atom ratios of 1.3, 2.1 and 2.8, respectively. Removal of adventitious copper results in a Cu:Fe:Zn stoichiometry of 2:2:1. The zinc is tightly bound. Dialysis against a solution of 1,10-phenanthroline at pH 7.4 or an acidic buffer (pH 4.4) does not remove Zn. Dialysis against 0.8 M KCN at pH 10 causes partial loss of both Cu and Zn. This is the first evidence for the presence of Zn in a cytochrome c oxidase. 相似文献
108.
光呼吸代谢物乙醇酸、乙醛酸和草酸对烟草叶片硝酸还原的影响 总被引:6,自引:0,他引:6
乙醇酸、乙醛酸和草酸能明显促进烟草(Nicotiana rustica)叶片在黑暗中的硝酸还原,光呼吸抑制剂a-羟基吡啶甲烷磺酸能消除前二者的促进作用而不能完全消除草酸的作用。草酸+NAD~+能显著促进离体的硝酸还原。烟叶提取液加入草酸和NAD~+后生成NADH和CO_2认为活体内由乙醛酸氧化生成的草酸是经脱氢生成NADH供硝酸还原之用。未能证明在烟叶内存在乙醇酸脱氨酶,因此排除由乙醇酸直接脱氢以还原硝酸的可能。 相似文献
109.
Abstract Sporopachydermia cereana , an ascosporogenous yeast, grew on dimethylamine, trimethylamine or trimethylamine N -oxide as sole nitrogen sources and produced mono-oxygenases for dimethylamine and trimethylamine that were significantly more stable than the corresponding enzymes found in Candida utilis . No trimethylamine mono-oxygenase activity was found in S. cereana grown on dimethylamine. In cells grown on trimethylamine N -oxide (but not on the other nitrogen sources), evidence for an enzyme metabolizing the N -oxide, possibly an aldolase, but more probably a reductase was obtained. All these activities showed a similar requirement for the presence of FAD or FMN in the extract buffer during isolation to retain activity. Amine mono-oxygenase activities showed a similar sensitivity to inhibitors, including proadifen hydrochloride and carbon monoxide as the corresponding enzymes in C. utilis . The trimethylamine N -oxide-dependent oxidation of NADH was more sensitive to inhibition by EDTA, N -ethylmaleimide and β-phenylethylamine than the mono-oxygenases, and less sensitive to KCN, and activity was significantly higher with NADPH than was observed with the 2 mono-oxygenases. 相似文献
110.
Abstract Nitrate reduction to ammonia by marine Vibrio species was studied in batch and continuous culture. In pH-controlled batch cultures (pH 7.4; 50 mM glucose, 20 mM KNO3 ), the nitrate consumed accumulated to more than 90% as nitrite. Under these conditions, the nitrite reductase (NO− 2 → NH3 ) was severely repressed. In pH-controlled continuous cultures of V. alginolyticus with glucose or glycerol as substrates ( D = 0.045 h−1 ) and limiting N-source (nitrate or nitrite), nitrite reductase was significantly derepressed with cellular activities in the range of 0.7–1.2 μmol min−1 (mg protein)−1 . The enzyme was purified close to electrophoretic homogeneity with catalytic activity concentrations of about 1800 nkat/mg protein. It catalyzed the reduction of nitrite to ammonia with dithionite-reduced viologen dyes or flavins as electron donors, had an M r of about 50 000 (determined by gel filtration) and contained c-type heme groups (probably 4–6 per molecule). 相似文献