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151.
Most spider threads are on the micrometre and sub-micrometre scale. Yet, there are some spiders that spin true nano-scale fibres such as the cribellate orb spider, Uloborus plumipes. Here, we analyse the highly specialized capture silk-spinning system of this spider and compare it with the silk extrusion systems of the more standard spider dragline threads. The cribellar silk extrusion system consists of tiny, morphologically basic glands each terminating through exceptionally long and narrow ducts in uniquely shaped silk outlets. Depending on spider size, hundreds to thousands of these outlet spigots cover the cribellum, a phylogenetically ancient spinning plate. We present details on the unique functional design of the cribellate gland–duct–spigot system and discuss design requirements for its specialist fibrils. The spinning of fibres on the nano-scale seems to have been facilitated by the evolution of a highly specialist way of direct spinning, which differs from the aqua-melt silk extrusion set-up more typical for other spiders. 相似文献
152.
E. Fernando Cagua Jesse E. M. Cochran Christoph A. Rohner Clare E. M. Prebble Tane H. Sinclair-Taylor Simon J. Pierce Michael L. Berumen 《Biology letters》2015,11(4)
Although whale sharks (Rhincodon typus) have been documented to move thousands of kilometres, they are most frequently observed at a few predictable seasonal aggregation sites. The absence of sharks at the surface during visual surveys has led to the assumption that sharks disperse to places unknown during the long ‘off-seasons’ at most of these locations. Here we compare 2 years of R. typus visual sighting records from Mafia Island in Tanzania to concurrent acoustic telemetry of tagged individuals. Sightings revealed a clear seasonal pattern with a peak between October and February and no sharks observed at other times. By contrast, acoustic telemetry demonstrated year-round residency of R. typus. The sharks use a different habitat in the off-season, swimming deeper and further away from shore, presumably in response to prey distributions. This behavioural change reduces the sharks'' visibility, giving the false impression that they have left the area. We demonstrate, for the first time to our knowledge, year-round residency of unprovisioned, individual R. typus at an aggregation site, and highlight the importance of using multiple techniques to study the movement ecology of marine megafauna. 相似文献
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156.
D.R. Dufield O.V. Nemirovskiy M.D. Tortorella W.R. Mathews 《Analytical biochemistry》2010,406(2):113-123
The degradation of articular cartilage by aggrecanases (ADAMTS-4 and ADAMTS-5) plays a significant role in the pathology of osteoarthritis (OA). To monitor aggrecanase activity in OA, we have developed a sensitive, accurate, and versatile assay for detection of two specific cleavage sites on aggrecan. The assay uses an immunoaffinity-based liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to detect cleavage at the 374ARGS site and the 1820AGEG site. The dynamic range of the assay is more than three orders of magnitude, with interassay precision less than 15%. It has been successfully applied to various biological fluids and species, including rat, bovine, dog, and human. The assay has been analytically qualified for use in human urine and synovial fluid (SF). The limits of detection (LODs) for ARGS in urine and SF are 2.5 and 10 pg/ml, respectively, whereas the LOD for AGEG is 20 pg/ml in SF. Analysis of these biomarkers from OA subjects and normal healthy volunteers revealed a significant elevation of both markers in OA. Similarly, in a rat model of cartilage degradation, both ARGS and AGEG were elevated, demonstrating the utility of these biomarkers for translational research. These data suggest that the ARGS and AGEG biomarkers developed have potential as measures of aggrecanase activity in OA and may contribute to our understanding of OA pathology. 相似文献
157.
Summary Estimation of abundance is important in both open and closed population capture–recapture analysis, but unmodeled heterogeneity of capture probability leads to negative bias in abundance estimates. This article defines and develops a suite of open population capture–recapture models using finite mixtures to model heterogeneity of capture and survival probabilities. Model comparisons and parameter estimation use likelihood‐based methods. A real example is analyzed, and simulations are used to check the main features of the heterogeneous models, especially the quality of estimation of abundance, survival, recruitment, and turnover. The two major advances in this article are the provision of realistic abundance estimates that take account of heterogenetiy of capture, and an appraisal of the amount of overestimation of survival arising from conditioning on the first capture when heterogeneity of survival is present. 相似文献
158.
Abstract: State wildlife agencies often use input obtained through public meetings to develop management policies. Because public meetings can be dominated by single stakeholder groups, these policies may not reflect the attitudes of new wildlife stakeholders. In 2000 the Utah Wildlife Board, after a series of public meetings, adopted a statewide policy for winter-feeding mule deer (Odocoileus hemionus). The policy was implemented by the Utah Division of Wildlife Resources from 2001 to 2007 in Cache County of northern Utah, USA. In 2007, we surveyed Utah households representing metropolitan, nonmetropolitan, and Cache County residents (n = 1,800) to evaluate whether the winter-feeding policy reflected the attitudes of all wildlife stakeholders. Survey respondents, regardless of residence strata, believed winterfeeding programs were essential for managing mule deer in Utah (χ26 = 7.02, P = 0.32). However, most respondents were reluctant to support feeding programs at the expense of habitat restoration projects (χ26 = 11.64, P = 0.07). Our results suggest that the winter-feeding policy represented the attitudes of the Utah residents surveyed, though few had participated in its development. Respondents' strong utilitarian attitudes toward wildlife (e.g., strong support for hunting and feeding) influenced those respondents' perceptions of the policy. Given the effects of increased urbanization on utilitarian attitudes toward wildlife in many parts of the United States, coupled with decreasing numbers of traditional wildlife stakeholders, state wildlife agencies should continually reevaluate their public involvement processes to ensure new wildlife stakeholders' attitudes and concerns are represented. 相似文献
159.
Abstract: We designed a reliable and inexpensive universal trap timer that records the time from the moment a single-live-capture trap is triggered by an animal to when the observer checks the trap. Combined with trapping information, the diel activity pattern of a given species or demographic group can then be described or compared between imposed treatments. The universal trap timer is adaptable to operate reliably with most single-capture trap designs, requires no permanent modification of traps, and is easy to construct. 相似文献
160.