心脉通胶囊联合非诺贝特对饮食控制疗法效果不佳的高脂血症患者血脂、血液流变学和血管内皮功能的影响

摘要 目的：观察心脉通胶囊联合非诺贝特对饮食控制疗法效果不佳的高脂血症患者血脂、血液流变学和血管内皮功能的影响。方法：所选病例为我院2019年5月~2021年9月期间接诊的68例饮食控制疗法效果不佳的高脂血症患者。采用随机数字表法进行分组,68例患者共分为对照组和联合组,例数各为34例。对照组患者接受非诺贝特治疗,联合组患者接受心脉通胶囊联合非诺贝特治疗,对比两组临床总有效率、血脂、血液流变学和血管内皮功能,记录两组治疗期间不良反应发生情况。结果：联合组的临床总有效率高于对照组（P<0.05）。治疗2个月后,联合组的血清全血还原黏度、低密度脂蛋白胆固醇（LDL-C）、血管内皮素-1（ET-1）、血浆比黏度、甘油三酯（TG）、全血比黏度、总胆固醇（TC）、纤维蛋白原水平、血细胞比容水平低于对照组,降钙素基因相关肽（CGRP）、高密度脂蛋白胆固醇（HDL-C）、一氧化氮（NO）水平高于对照组（P<0.05）。两组不良反应发生率对比无统计学差异（P>0.05）。结论：心脉通胶囊联合非诺贝特治疗饮食控制疗法效果不佳的高脂血症患者,可有效控制血脂,调节血液流变学和血管内皮功能,应用价值较好。     

腰痹通胶囊与布洛芬缓释胶囊联合用药治疗慢性腰痛疗效的随机对照研究

摘要 目的：观察腰痹通胶囊与布洛芬缓释胶囊联合用药治疗慢性腰痛的临床疗效。方法：将168例在我院门诊接受治疗的慢性腰痛患者随机分为A组、B组与C组（各56例）,A组给予口服腰痹通胶囊 (3粒/次),3次/d,饭后服药;B组给予布洛芬缓释胶囊治疗 (300 mg/次),2次/d,饭后口服;C组同时口服腰痹通胶囊和布洛芬缓释胶囊（用法同前）,3组均治疗1个疗程。观察治疗前后3组患者的临床疗效、视觉模拟评分（VAS）、Oswestry功能障碍指数（ODI）评分、日本骨科协会腰椎治疗评价量表（JOA）评分、血清炎性因子和药物副作用等各项指标,并进行对比分析。结果：所有患者均完成了研究,没有退出或脱落病例。A组的总有效率为76.79%,B组的总有效率为82.14%,C组的总有效率为96.43%,经统计学分析,AB两组之间没有显著的统计学差异（P>0.05）,而C组与A组或B组之间均有显著性差异（P<0.05）。3组的VAS、ODI、JOA评分在治疗前后具有显著的统计学差异（P<0.05）,其中C组治疗后的相关评分优于其他两组（P<0.05）。C组患者治疗后的炎性因子包括C反应蛋白(CRP)、白介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)水平均低于A组、B组(P<0.05)。A组的药物副作用最少,B组的药物副作用最多,A组和C组的不良反应发生率低于B组（P<0.05）。结论：腰痹通胶囊联合布洛芬缓释胶囊口服治疗慢性腰痛的疗效明显优于这两种药物单独使用,可缓解患者腰部疼痛,改善腰椎功能,降低血清炎性因子水平。     

哺乳期需氧菌性阴道炎患者定期补充乳杆菌的疗效

目的分析在有效抗炎基础上定期补充乳杆菌活菌胶囊对哺乳期需氧菌性阴道炎患者的疗效及对远期复发率的影响。方法选择2019年4月至2020年3月于大连市妇女儿童医疗中心(集团)行产后42 d检查时发现的需氧菌性阴道炎患者共120例,按照随机自愿原则将所选患者分为观察组及对照组,各60例。全部患者均使用红核妇洁洗液及克林霉素磷酸酯阴道凝胶抗炎治疗7 d,观察组患者于第8天开始给予阴道用乳杆菌活菌胶囊每日睡前上药1粒,治疗10 d,停药后1周对两组患者进行临床疗效评估并了解不良反应发生情况。于首次治疗结束后第3个月及第6个月进行定期随诊,了解两组患者阴道炎的复发情况。且观察组患者于治疗后第3个月随诊结束后再次给予阴道用乳杆菌活菌胶囊补充上药1疗程。记录并比较两组患者治疗前、治疗后1周、治疗后第3个月及第6个月阴道pH值及H₂O₂情况。结果两组患者治疗期间均未发生明显不良反应。治疗后1周观察组患者治愈率(66.7%)显著高于对照组(36.7%),差异有统计学意义(P<0.05);观察组总有效率(95.0%)与对照组(86.7%)比较差异无统计学意义...     

Distribution of actin bundles in Bowman's capsule of rat kidney

In this study we define the distribution of actin bundle arrangement in Bowman's capsule of rat renal corpuscles. Parietal cells of Bowman's capsule were examined by conventional light microscopy, electron microscopy and confocal microscopy. Within each parietal cell individual actin bundles are arranged in a parallel fashion running the length of the cell. Computer reconstructions obtained using confocal microscopy clearly show the lengths of actin bundles to be arranged, on a capsule level, end-to-end, at angles and perpendicular to bundles in adjacent cells. The bundles stain positively for non-muscle myosin and vinculin. The presence and arrangement of actin bundles in parietal cells is consistent with a role in reinforcing capsule structure.     

Hyaluronan release from Streptococcus pyogenes: export by an ABC transporter

Capsular hyaluronan of Streptococcus pyogenes is synthesized at the protoplast membrane. It is widely assumed that hyaluronan is exported by the synthase itself and that no additional protein is required for transfer through plasma membranes. However, we produced an insertional mutation that reduced the mucoid phenotype, hyaluronan production, and capsule formation. Nucleotide sequence analysis of the insertion site identified a gene coding for a protein with an ATP-binding cassette (ABC) that belonged to an ABC transporter system and was located next to the hyaluronan synthesis genes. The mucoid phenotype was reconstituted by complementation with DNA encoding the ABC transporter system. These results indicated that an ABC transporter was required for efficient capsule production.     

Splash seed dispersal by raindrops

Splash seed dispersal by raindrops was investigated for plants in southern Japan. Nine families, 10 genera and 19 species were confirmed as raindrop-dispersed plants. The 10 genera were Gentiana, Gratiola, Chrysosplenium, Mazus, Mitella, Ophiorrhiza, Sagina, Sedum, Trigonotis and Veronica. The method of splash rain dispersal in these species was clarified. Raindrop-dispersed species were all small herbaceous plants with a vertical pedicel and an apically opening fresh capsule when the seeds mature. Open capsules were cup-shaped or boat-shaped and can accommodate raindrops easily. The raindrops splashed the seeds from the capsule. In general, the seeds weighed very little, but they were heavier than powder or dust seeds dispersed by wind. A strong negative correlation was found between seed weight and the number of seeds per capsule. In the case of Trigonotis brevipes (Maxim.) Maxim., raindrops were received into the cup-shaped calyx-tube and dispersed the fruitlets. Some species, such as Gentiana thunbergii (G. Don) Griseb., Gentiana zollingeri Fawcett and Ophiorrhiza japonica Blume, had hydroscopic movement capsules that opened widely only when wet. Raindrop-dispersed plants were found in various habitats. For example, some plants grew together on rocks along the mountain torrents where splash water could easily be caught. The results of the laboratory and field experiments indicated that the dispersal distance of seeds by raindrops was 1m or less. For small herbaceous plants, splash dispersal by rain might be an effective and advantageous method of seed dispersal because dispersal is not affected by plant height.     

Purification and characterization of KpsT,the ATP-binding component of the ABC-capsule exporter of Escherichia coli K1

The K1 capsule, an alpha(2,8)-linked polymer of sialic acid, is an important virulence determinant of invasive Escherichia coli. The 17-kb kps gene cluster of E. coli K1 encodes the information necessary for capsule expression at the cell surface. Two proteins, KpsM and KpsT, play a role in the transport of capsular polysaccharide across the cytoplasmic membrane, utilizing the energy from ATP hydrolysis. They belong to the ATP-binding cassette superfamily of transport proteins. In this study, we purified KpsT in its native form and show that the purified protein is able to bind ATP, undergo an ATP-dependent conformational change and hydrolyze ATP. Protease accessibility studies demonstrate the in vivo interaction between KpsM and KpsT.     

Influences of milk components on biofilm formation of Cronobacter spp. (Enterobacter sakazakii)

Aim:  To determine the critical component(s) of skim milk for biofilm formation of Cronobacter species.
Methods and Results:  Biofilm forming ability of 72 Cronobacter strains in skim milk preparation was assayed by crystal violet staining. The results revealed that whey protein and casein are more important determinants of skim milk for biofilm formation than lactose, although there was a wide variation in biofilm forming ability. Biofilm structure and capsular material of six strains exhibiting different biofilm forming ability was investigated via electron microscopes. Scanning electron microscopy showed visually that while the strong biofilm formers (E27B, FSM 30 and 2·82) resulted in almost complete coagulation of skim milk, the weak biofilm formers (55, FSM 290 and 2·84) caused less coagulation. No capsule was clearly delineated in transmission electron micrographs of either strong or weak biofilm formers.
Conclusion:  These results indicate that, for biofilm formation of Cronobacter species in skim milk, nitrogen source is probably a more important determinant than carbohydrate, and that strong biofilm formers are responsible for substantial coagulation of skim milk.
Significance and Impact of the Study:  This study provides information for better understanding of the underlying mechanisms by which Cronobacter species form biofilm in infant formula milk.     

Rodlet cells in epidermal explant cultures of Lepomis Macrochirus

Sunfish rodlet cells were examined in vitro using a novel tissue explant system. Outgrowth of epidermal cell layers from explanted fish scales enabled both live cell videomicroscopy and immunocytochemical analysis of rodlet cells within the cell layer. Cells stained with fluorescent phallotoxin and antibody to tubulin showed that F‐actin is a component of the fibrous capsule that envelopes the cell and a microtubule network extends from the basal to apical ends of the cell interior. The fibrous capsule is also enriched for phosphotyrosine suggesting a potential signal‐transducing capability is present in this structure. Videomicroscopy analysis of live explant cultures demonstrated that rodlet cells are immobile and that interior structures are highly dynamic. Rodlet sacs can undergo extension and retraction, while intracellular particles can move rapidly within these cells. Fish scale tissue explants provide a useful system for analyzing the molecular composition and dynamic behavior of rodlet cells.     

Highly selective screening of the bioactive compounds in Huoxue capsule using immobilized β2-adrenoceptor affinity chromatography

A highly selective assay was developed for screening compounds that bind to the porcine recombinant β₂-adrenoceptor (β₂-AR) with affinity chromatography coupled to quadrupole time-of-flight mass spectrometry (Q-TOF–MS). The methodology involved selective screening with immobilized β₂-AR, a highly accurate identification via Q-TOF–MS, and a functional evaluation of the screened compounds with a sensitive myograph system. Ferulic acid, hydroxysafflor yellow A (HSYA), and naringin were confirmed to be the bioactive compounds in Huoxue capsule that specifically bound to the β₂-AR. These compounds produced a concentration-dependent relaxation of arteries that were contracted by treatment with phenylephrine, and the relaxation caused by these compounds was attenuated in the presence of ICI 118551, a type of β₂-AR antagonist. Our data indicate that the use of an immobilized receptor is potentially an alternative method for the rapid screening of bioactive compounds in a complex matrix because of its high specificity. β₂-AR affinity chromatography was valuable in focusing attention on the further investigation of ferulic acid, HSYA, and naringin as β₂-AR agonists.