白粒济宁3号小麦品种的选育

用快中子照射红粒济宁3号小麦干种子,后代获得白粒植株,经选育而成白粒济宁3号。白粒济宁3号与红粒济宁3号比,除籽粒颜色差别外,籽粒蛋白含量显著高于红粒济宁3号,而落黄性、籽粒饱满度、千粒重、产量均略低于红粒济宁3号,该品种累计种植面积10余万亩。     

多聚半乳糖醛酸内切酶的固定化及其性质研究

棉花枯萎病菌多聚半乳糖醛酸内切酶在pH大于7时不稳定,故对它进行多种化学修饰而又不影响其活性,必须在pHd小于7的体系中进行。本文报道将PGAUase在还原剂存在下,与稀酸处理的Sepharose 4B交联,获得较高活力的固定化酶。固定化酶催化动力学表明,最适pH为4,4,最适温度为55℃,在pH1至8.0范围内稳定。和溶液酶比较,对热稳定性提高,但对碱稳定性下降。以多聚半乳糖醛酸为底物,Km为0.27mmol/L,Vmax为66.67nmol/L·min,均大于溶液酶(Km=0.07mmol/L,Vmax=28.00nmol/L·min)。在pH4.8,30℃,聚半乳糖醛酸在固相酶的柱中循环水解不同的时间降解产物经圆盘电泳和等电聚焦测定,得到不同大小的寡糖片段混合物,证明固相酶和溶液酶的作用方式相同,同时使以酶解法制备一定大小的有生物活性的寡糖分子成为可能。     

大鼠肾脏和其它组织中磷酸酪氨酸蛋白的研究

本文采用P-tyr-BSA为免疫原免疫家无得抗血清。将纯化的IgG与HRP偶联,建立了P-tyr-Pr的ELISA法,并测定了正常大鼠肾脏等组织中P-tyr-Pr含量,其分布规律如下:上清中P-tyr-Pr含量高者,其颗粒部分则低,反之亦然;其中肾脏上清中含量远比其它组织(脾、肺、肝等)高。在此基础上,又研究了膜性肾炎大鼠肾脏P-tyr-Pr含量,发现其上清中的含量远远高于正常大鼠肾脏中的含量。     

Hydrolysis of Butteroil by Immobilized Lipase Using a Hollow-Fiber Reactor: Part VI. Multiresponse Analyses of Temperature and pH Effects

A lipase from Aspergillus niger, immobilized by physical adsorption on hydrophobic hollow fibers made of microporous polypropylene, was used to effect the hydrolysis of the glycerides of melted butterfat at 40, 50, 55, and 60°C (pH 7.0), and at pH 3.0, 4.0, 5.0, 7.0, 8.0, and 9.0 (40°C). McIlvane buffer and melted butterfat were pumped cocurrently through the hollow fiber reactor. The concentrations of ten different free fatty acids in the effluent oil stream were measured by HPLC. Multiresponse nonlinear regression methods were employed to fit the data to multisubstrate rate expressions derived from a Ping Pong Bi Bi mechanism in which the rate controlling step is deacylation of the enzyme. Thermal deactivation of the immobilized lipase was also included in the mathematical model of reactor performance. A postulated normal distribution of v_max with respect to the number of carbon atoms of the fatty acid residue (with an additive correction for the number of double bonds) was found to provide the best statistical fit of the data. The models developed can be used to independently predict the effects of either the pH or the temperature, as well as the reactor space time and the time elapsed after immobilization, on the free fatty acid profile of the lipolyzed butteroil product.     

Multiple origins of self-fertilization in tristylous Eichhornia paniculata (Pontederiaceae): Inferences from style morph and isozyme variation

Populations of Eichhornia paniculata (Pontederiaceae) exhibit a wide range of mating systems, from predominant outcrossing to high levels of self-fertilization. The origin of self-fertilization in this tristylous species is associated with the loss of style-length morphs from populations and the spread of self-pollinating, floral variants. We examined geographic variation in style morph and allozyme frequencies to determine whether the loss of style morphs and transition to selfing could have multiple origins in E. paniculata. Surveys of floral variation in 167 populations from six states in northeastern Brazil revealed that at least one style morph was absent from 29.3%. Non-trimorphic populations occurred in all states and ranged in frequency from 9% in Ceará to 68% in Alagoas. Selfing variants occurred in 8.5% and 55% of trimorphic and non-trimorphic populations, respectively, and were distributed among five of six states with primary concentrations in Alagoas and Pernambuco. A comparison of electrophoretic variation at 24 isozyme loci in 28 trimorphic, 13 dimorphic and 3 monomorphic populations indicated that non-trimorphic populations contained 84% of the allelic variation present in trimorphic populations and were markedly differentiated from one another. Analyses of genetic distance and the distribution of rare alleles indicated that non-trimorphic populations were often more similar to neighbouring trimorphic populations than to one another. Populations with selfing variants occurred at low frequency in three genetically distinct parts of the range. These results, in combination with genetic and morphological evidence suggest that style morphs are lost repeatedly from populations of E. paniculata and that selfing variants may have originated on at least three separate occasions in northeastern Brazil.     

我国大麦和性花叶病毒（BaMMV）研究初报

本文根据F(ab′)2－ELISA和ISEM实验,结合病毒粒子长度测定,报道了江苏省如东县农科所大麦黄花病毒源（吕种盐辐矮早3）中除存在大麦花花叶病毒（BaYMV）外,还复合感染大麦和性花叶病毒（BaMMV）,这是我国BaMMV的第一次报道。     

迎接廿一世纪——记中国无锡第十一届国际植物园协会大会

第十一届国际植物园协会（IABG）大会于1993年9月7日在中国无锡开幕,经苏州、杭州,于9月12日在上海闭幕。9月6日部分与会代表参加了在南京举行的“中国南京国际植物园俱乐部”奠基仪式及江苏省重点实验室南京中山植物园“植物迁地保存实验室”扩建部分开放使用等一系列庆祝活动。参加大会的有来自23个国家和地区的199名代表。大会收到论文74篇,其中板报42篇,大会报告13篇。本次会议的主要议题为“植物园与国家发展”。经过大会报告和交流,明确了世界植物园在继续发挥其保护物种和保护环境的功能之外,必须进一步密切植物园与国家发展的关系。全面发展植物园的十项功能,不仅可以增加植物园对社会的贡献,也有利于提高植物园自身的活力和生存能力。大会选出了以主席岩（木规）邦男,副主席李蔼娃、贺善安、安德列也夫,秘书长艾斯特万为领导核心的新一届理事会。世界植物园两大组织国际植物园协会（IABG）和国际植物园保护组织（BGCI）在无锡签署的合作备忘录标志着国际植物园运动进入了一个新的历史时期。理事会的第一次会议于9月11日在由杭州开往上海的火车上举行。讨论了整顿组织、会员登记、出版IABG通讯及与BGCI合作开展活动等问题。会议确认1994年将在印尼召开IABG-AD第二次会议。第十二届IABG大会将于1     

赣榆县厉庄地埂利用型式试验

在赣榆县厉庄以大金鸡菊、紫穗槐和红柳三种经济植物进行了地埂利用型式试验,取得了比较明显的水土保持效果和改善农田小环境的生态效益。栽植后第三年植物覆盖率达90％或100％;地面蒸发量减少15.2～17.7％;截留降水量为0.5～0.7mm;大气相对湿度提高3.7～5.4％;土壤养分也有不同程度的增加。经济效益估测每亩地埂可收到300～600元,比种黄豆高5～9倍。效益最好的是红柳,大金鸡菊次之,紫穗槐较低。     

菊花离体快速繁殖的研究

用MS基本培养基附加植物生长调节剂,进行了菊花离体快速繁殖试验。以幼叶为外植体在附加不同生长素的培寿基上诱导愈伤组织后进行分化培养。结果表明,2.OppmNAA上的愈伤组织诱导率最高,0.5ppmBA上的不定芽分化率最高。用不同浓度NAA与BA的组合进行分化试验,其方差分析结果说明0.1ppmNAA+0.5ppmBA是分化培养的最佳激素组合。无根苗在MS无激素培寿基上诱导生根,28天后移栽成活率达到85％。     

慈竹无性系种群能值特点及其影响能值计测因素的研究

 本文引用Harper(1977)的构件结构理论,从构件结构单位、无性系分株和无性系三个层次,对四川南充市郊慈竹无性系种群的能值特点及其影响能值的计测因素进行了定量研究。研究结果表明：慈竹无性系种群中,各构件单位的去灰分热值(AFCV)分别为：根15349.42J／g、根茎16372.92J／g、秆17106.06J／g、枝18111.99J／g和叶19451.90J／g;慈竹无性系分株的AFCV(J／g)随龄级增大而递增;慈竹无性系水平上的AFCV为：Ⅰ龄占16.47%、Ⅱ龄占25.76%、Ⅲ龄占36.32%、Ⅳ龄为13.08%及Ⅴ龄为8.37%。用恒容燃烧法测定热值时,其能值变化与氧分压密切相关。用经验公式计算的能值较作图法高;用AFCV表示能值较总干重热值(GCV)准确。