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121.
Johan U. Grobbelaar 《Journal of applied phycology》1989,1(4):333-340
It has been suggested that turbulence with the resultant light/dark cycle and light gradient through which phytoplankton move,
enhances their productivity. The stationary bottle incubation technique for estimating rates of primary productivity has mainly
been criticized because of bottle effects, the elimination of natural turbulence and the presence of photo-inhibition. In
a series of experiments where productivity was measured over static profiles and compared to the productivity in a mixed system,
no definite conclusion could be reached regarding the effect of varying light/dark cycles of medium frequency (seconds to
minutes). It appeared as though the ratio of the euphotic depth to mixing depth (Z
eu/Z
m) influenced productivity more than the duration of the light/dark cycle. The static bottle incubation method gave higher
integral productivities than the mixed samples at low ratio's ofZ
eu/Z
m. It is suggested that mixing has two separate, but synergistic effects i.e. it not only moves the phytoplankton cells through
a light/dark cycle, but also decreases the boundary layer, which increases the rate of exchange through the cell wall of nutrients
and metabolites. In doing so more nutrients are available and light could be utilized more efficiently and therefore, productivity
is increased. 相似文献
122.
At the developmental stage at which the apical hook passed the 3rd and 4th nodes, dark-grown seedlings of pea ( Pisum sativum L. cv. Progress No.9) opened the hook upright and then formed a new hook above the node nearly in the opposite direction to the previous one. In cv. Alaska, in contrast, many (about 84%) seedlings closed the hook in the original direction after they partially (up to about 110°) opened it at the 3rd node, thus doing a wagging movement, while a small percentage (about 16%) of the seedlings reversed the hook direction. Exposure to red light of cv. Alaska seedlings for 10 min increased the percentage of the hook reversion up to 71% or more. The hook reversion was never observed except when the hook part passed the nodes, suggesting the involvement of the nodes in the phenomenon. 相似文献
123.
Gravitropic stimulation of maize (Zea mays L.) seedlings resulted in a continuous curvature of the coleoptiles in a direction opposing the vector of gravity when the seedlings were rotated on a horizontal clinostat. The orientation of this response, however, was reversed when the gravitropic stimulation was preceeded by symmetric preirradiation with blue light (12.7 mol photons·m–2). The fluence-response curve of this blue light exhibited a lower threshold at 0.5 mol·m–2, and could be separated into two parts: fluences exceeding 5 mol·m–2 reversed the direction of the gravitropic response, whereas for a range between the threshold and 4 mol·m–2 a split population was obtained. In all cases a very strong curvature resulted either in the direction of gravity or in the opposite orientation. A minor fraction of seedlings, however, curved towards the caryopsis. Furthermore, the capacity of blue light to reverse the direction of the gravitropic response disappeared with the duration of gravitropic stimulation and it depended on the delay time between both stimulations. Thistonic blue-light influence appears to be transient, which is in contrast to the stability observed fortropistic blue-light effects.This work was supported by the Deutsche Forschungsgemeinschaft. 相似文献
124.
Stomatal response to humidity in sugarcane and soybean: effect of vapour pressure difference on the kinetics of the blue light response 总被引:7,自引:2,他引:5
Abstract. The effect of atmospheric humidity on the kinetics of stomatal responses was quantified in gas exchange experiments using sugarcane ( Saccharum spp. hybrid) and soybean ( Glycine max ). Pulses of blue light were used to elicit pulses of stomatal conductance that were mediated by the specific blue light response of guard cells. Kinetic parameters of the conductance response were more closely related to leaf-air vapour pressure difference (VPD) than to relative humidity or transpiration. Increasing VPD significantly accelerated stomatal opening in both sugarcane and soybean, despite an approximately five-fold faster response in sugarcane. In contrast, the kinetics of stomatal recovery (closure) following the pulse were similar in the two species. Acceleration of opening by high VPD was observed even under conditions where soybean exhibited a feedforward response of decreasing transpiration (E) with increasing evaporative demand (VPD). This result suggests that epidermal, rather than bulk leaf, water status mediates the VPD effect on stomatal kinetics. The data are consistent with the hypothesis that increased cpidermal water loss at high VPD decreases the backpressure exerted by neighbouring cells on guard cells. allowing more rapid stomatal opening per unit of guard cell metabolic response to blue light. 相似文献
125.
Acclimation of barley to changes in light intensity: photosynthetic electron transport activity and components 总被引:9,自引:0,他引:9
Barley seedlings (Hordeum vulgare L. Boone) were grown at 20°C with 16 h/8 h light/dark cycle of either high (H) intensity (500 mole m-2 s-1) or low (L) intensity (55 mole m-2 s-1) white light. Plants were transferred from high to low (H L) and low to high (L H) light intensity at various times from 4 to 8 d after leaf emergence from the soil. Primary leaves were harvested at the beginning of the photoperiod. Thylakoid membranes were isolated from 3 cm apical segments and assayed for photosynthetic electron transport, Photosystem II (PS II) atrazine-binding sites (QB), cytochrome f(Cytf) and the P-700 reaction center of Photosystem I (PS I). Whole chain, PS I and PS II electron transport activities were higher in H than in L controls. QB and Cytf were elevated in H plants compared with L plants. The acclimation of H L plants to low light occurred slowly over a period of 7 days and resulted in decreased whole chain and PS II electron transport with variable effects on PS I activity. The decrease in electron transport of H L plants was associated with a decrease in both QB and Cytf. In L H plants, acclimation to high light occurred slowly over a period of 7 days with increased whole chain, PS I and PS II activities. The increase in L H electron transport was associated with increased levels of QB and Cytf. In contrast to the light intensity effects on QB levels, the P-700 content was similar in both control and transferred plants. Therefore, PS II/PS I ratios were dependent on light environment.Abbreviations Asc
ascorbate
- BQ
2,5-dimethyl-p-benzoquinone
- DBMIB
2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone
- DCIP
2,6-dichlorophenolindophenol
- H
control plants grown under high light intensity
- H L
plants transferred from high to low light intensity
- L
low control plants grown under low light intensity
- L H
plants transferred from low to high light intensity
- MV
methyl viologen
- P-700
photoreaction center of Photosystem I
- QB
atrazine binding site
- TMPD
N,N,N,N-tetramethyl-p-phenylenediamine
Cooperative investigations of the United States Department of Agriculture, Agricultural Research Service, and the North Carolina Agricultural Research Service, Raleigh, NC. Paper No. 11990 of the Journal Series of the North Carolina Agricultural Research Service, Raleigh, NC 27695-7643, USA. 相似文献
126.
When excited by ultraviolet radiation, leaves of a great number of species of higher plants exhibit emission of blue fluorescence, comparable in intensity to the red emission of chlorophyll. The fluorescence decay of the blue emission of spinach leaves recorded by single photon counting techniques is decomposed into exponential components and it is shown that at least three different components are present. The lifetime of the three components does not show significant variations with the excitation or emission wavelengths. The excitation and emission spectra of each component were determined. The nature of the chemical compounds which cause this emission is discussed in relation to these spectra. 相似文献
127.
The possibility that zeaxanthin mediates the dissipation of an excess of excitation energy in the antenna chlorophyll of the photochemical apparatus has been tested through the use of an inhibitor of violaxanthin de-epoxidation, dithiothreitol (DTT), as well as through the comparison of two closely related organisms (green and blue-green algal lichens), one of which (blue-green algal lichen) naturally lacks the xanthophyll cycle. In spinach leaves, DTT inhibited a major component of the rapidly relaxing high-energy-state quenching' of chlorophyll fluorescence, which was associated with a quenching of the level of initial fluorescence (F0) and exhibited a close correlation with the zeaxanthin content of leaves when fluorescence quenching was expressed as the rate constant for radiationless energy dissipation in the antenna chlorophyll. Green algal lichens, which possess the xanthophyll cycle, exhibited the same type of fluorescence quenching as that observed in leaves. Two groups of blue-green algal lichens were used for a comparison with these green algal lichens. A group of zeaxanthin-free blue-green algal lichens did not exhibit the type of chlorophyll fluorescence quenching indicative of energy dissipation in the pigment bed. In contrast, a group of blue-green algal lichens which had formed zeaxanthin slowly through reactions other than the xanthophyll cycle, did show a very similar response to that of leaves and green algal lichens. Fluorescence quenching indicative of radiationless energy dissipation in the antenna chlorophyll was the predominant component of high-energy-state quenching in spinach leaves under conditions allowing for high rates of steady-state photosynthesis. A second, but distinctly different type of high-energy-state quenching of chlorophyll fluorescence, which was not inhibited by DTT (i.e., it was zeaxanthin independent) and which is possibly associated with the photosystem II reaction center, occurred in addition to that associated with zeaxanthin in leaves under a range of conditions which were less favorable for linear photosynthetic electron flow. In intact chloroplasts isolated from (zeaxanthin-free) spinach leaves a combination of these two types of rapidly reversible fluorescence quenching occurred under all conditions examined.Abbreviations DTT
dithiothreitol
- F0 (or F0)
yield of instantaneous fluorescence at open PS II reaction centers in the dark (or during actinic illumination)
- FM (or FM)
yield of maximum fluorescence induced by a saturation pulse of light in the dark (or during actinic illumination)
- FV (or FV)
yield of variable fluorescence induced by a saturating pulse of light in the dark (or during actinic illumination)
-
k
D
rate constant for radiationless energy dissipation in the antenna chlorophyll
- SV
Stern-Volmer equation
- PFD
photon flux density
- PS I
photosystem I
- PS II
photosystem II
- QA
acceptor of photosystem II
- qN
coefficient of nonphotochemical chlorophyll fluorescence quenching
- qP
coefficient of photochemical chlorophyll fluorescence quenching 相似文献
128.
叶片叶肉结构对环境光强的适应及对光合作用的影响 总被引:16,自引:0,他引:16
本文利用Kubelka-Munk理论描述了平行光在叶片内的吸收和散射,同时利用叶片分层光合作用非直角双曲线光反应模型,给出了整张叶片光合作用计算式。最后利用优化理论阐明了叶片叶肉分化成光合特性具有明显差异的栅栏组织和海绵组织可能是对叶片内光梯度的一种适应;同时证明了叶片叶肉在一定环境光强下存在一个最佳的栅栏组织和海绵组织比例,并且这个比例随环境光强增大而增大,这最佳比例也受叶肉组织光合特性差异的影响。 相似文献
129.
Richard W. Joyiv Trevor A. Thorpe 《In vitro cellular & developmental biology. Plant》1990,26(6):643-646
Summary The effects of excision, light and cytokinin (N6-benzyladenine) on14C-acetate metabolism in cotyledons ofPinus radiata (D. Don) were determined.14CO2 was released and the distribution of radioactivity into lipids, sugars, organic acids and amino acids was determined. While
light and cytokinin generally caused some increase in metabolism, the effect of excision, i.e., wounding, was most pronounced.
Specific metabolites examined (citrate, malate, succinate, alanine, aspartate, glutamate and glutamine) were at least 50%
greater in14C-labeling in excised cotyledons as compared to intact seedlings. This enhancement of wound metabolism would mask possible
morphogenically-related changes occurring at that time.
This research was supported by the Natural Sciences and Engineering Research Council of Canada Grant A-6467 to T.A. Thorpe. 相似文献
130.
外源GA_3和红光都能有效地诱导台湾红番茄种子萌发;外源ABA对光诱导萌发有明显的抑制作用。白光和红光诱导萌发过程中内源ABA水平急剧降低,而在黑暗或远红光下则一直保持在与风干种子中原有的高水平。1 mol/L HCl酸化1h后加100 ppm GA_3诱导萌发时内源ABA含量也随培养时间的延续而明显下降。 相似文献