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961.
ALESSANDRA FIDELIS GERHARD E. OVERBECK VALÉRIO D. PILLAR JÖRG PFADENHAUER 《Austral ecology》2009,34(5):558-566
The role of facilitation in the structuring of plant communities has been often demonstrated in environments under high abiotic stress, especially in semi‐arid and arid ecosystems and high elevations. Few studies, however, analysed facilitation in systems that are highly productive and rich in species, which are thought to be theoretically unlikely to demonstrate strong effects of facilitation. Here, we investigate the importance of Eryngium horridum, a rosette species, on the maintenance of plant diversity in subtropical grasslands in southern Brazil. We evaluated facilitation in areas under two different types of management: abandonment and grazing. Plots were established in areas with and without individuals of E. horridum and all species were identified and had their cover estimated. The Relative Neighbour Effect index was calculated in order to verify the presence of competition or facilitation. Our results indicated facilitation in both abandoned and in grazed grasslands, but apparently through different mechanisms. In the first case, the plant's architecture opens the canopy and allows more light to reach small forbs in the grass matrix. In the second case, E. horridum appears to protect more palatable species from herbivores. Otherwise considered an obnoxious species, E. horridum plays an important ecological role in subtropical grasslands in southern Brazil by facilitating other species and consequently, increasing local richness. Areas with this rosette species are important sources of diaspores, which are able to colonize new open sites and thus, maintain biodiversity. 相似文献
962.
963.
Murilo S. Dias Ian F. de Faria Paula C. P. Guarido Fabrício B. Teresa Pedro de Podestà Uchôa de Aquino Juan P. Quimbayo 《Journal of fish biology》2020,96(4):877-885
Humans introduce non-native species by means such as the deliberate release of fish into fresh waters and through commercial trade. The guppy Poecilia reticulata Peters, 1859, is commonly kept in aquaria and controls disease vectors, and now it occurs in many areas outside its natural distribution. Its initial habitat in Brazil was identified, and a study was performed to determine whether the density of guppies can be explained by the density of human population, per-capita gross domestic product, level of human impact on the areas where guppies have been found and fish-sampling effort. A total of 1402 guppy records were found; the southeastern region had the oldest records; and the southeastern, northeastern and central-western regions had the maximum records. Low tolerance to the colder climate may be the reason for the lack of guppy records in the southernmost states. It was also observed that the occurrence of this fish is positively, yet weakly, related to the density of human population, indicating that improved regulations regarding its use in controlling disease vectors, the aquarium trade and education of aquarium hobbyists could help prevent the spread of this species and its potential impacts in Brazil. 相似文献
964.
An incubation method was used to investigate the nitrogen release characteristics from the residue of ten plant species which commonly grow in the northern part of the Loess Plateau. The effect of the residue on soil microbial biomass carbon (SMBC) and soil microbial biomass nitrogen (SMBN) was also determined. There were significant differences in the total N content and the C/N ratios among the different types of plant residue. The total N content of the residues ranged from 6.61 to 32.78 g kg?1. The C/N ratio of the residue ranged from 14 to 65. There was an immediate increase in soil N after alfalfa, erect milkvetch, and korshinsk peashrub residue was added to the soil. In contrast, soil N decreased after elm, sea buckthorn, and wild peach residue was added to the soil. The soil N content remained relatively low for 14–34 days and then increased. This indicated that N immobilization occurred during the early portion of the incubation period when elm, sea buckthorn and wild peach residue was added to the soil. Soil N levels were low during the entire incubation period when simon poplar, locust, Stipa bungeana, and old world bluestem residue were added to the soil. The addition of plant residue significantly increased SMBC and SMBN in all treatments. The SMBC and SMBN values were greatest in treatments containing plant residue with high total N content and low C/N ratios. The C/N ratios of korshinsk peashrub, sea buckthorn, and wild peach residues were similar, but the amount of N released from these residues and the effects of the residue on SMBC and SMBN in soil were significantly different. This indicates that not only the C/N ratio but also the chemical composition of the plant residue affected decomposition. It is important to consider C and N release characteristics from plant residue in order to adjust the C and N balance of soil when revegetating degraded ecosystems. 相似文献
965.
Understanding the spatial dynamics of landscape use by free-ranging herbivores is integral for successful ecosystem management. We used binary logistic regression analyses to determine the relative importance of biotic, abiotic and human factors in influencing the distribution (presence/absence) of wild grazers on two Maasai ranches in Kenya's Amboseli-Tsavo ecosystem. Both ranches had low grass biomass and suffered from regular droughts. We found that grazers consistently located themselves where grass biomass was highest, usually irrespective of grass quality, suggesting that forage quantity may be the limiting factor where grass biomass is generally low. The availability of surface water had no significant effect on the likelihood of grazers being present, even in the dry season. 相似文献
966.
W. D. Kissling R. Field H. Korntheuer U. Heyder K. B?hning-Gaese 《Philosophical transactions of the Royal Society of London. Series B, Biological sciences》2010,365(1549):2035-2045
Current methods of assessing climate-induced shifts of species distributions rarely account for species interactions and usually ignore potential differences in response times of interacting taxa to climate change. Here, we used species-richness data from 1005 breeding bird and 1417 woody plant species in Kenya and employed model-averaged coefficients from regression models and median climatic forecasts assembled across 15 climate-change scenarios to predict bird species richness under climate change. Forecasts assuming an instantaneous response of woody plants and birds to climate change suggested increases in future bird species richness across most of Kenya whereas forecasts assuming strongly lagged woody plant responses to climate change indicated a reversed trend, i.e. reduced bird species richness. Uncertainties in predictions of future bird species richness were geographically structured, mainly owing to uncertainties in projected precipitation changes. We conclude that assessments of future species responses to climate change are very sensitive to current uncertainties in regional climate-change projections, and to the inclusion or not of time-lagged interacting taxa. We expect even stronger effects for more specialized plant–animal associations. Given the slow response time of woody plant distributions to climate change, current estimates of future biodiversity of many animal taxa may be both biased and too optimistic. 相似文献
967.
Mark A. Harwell John H. Gentile Charles B. Johnson David L. Garshelis Keith R. Parker 《人类与生态风险评估》2010,16(4):727-761
A comprehensive, quantitative risk assessment is presented of the toxicological risks from buried Exxon Valdez subsurface oil residues (SSOR) to a subpopulation of sea otters (Enhydra lutris) at Northern Knight Island (NKI) in Prince William Sound, Alaska, as it has been asserted that this subpopulation of sea otters may be experiencing adverse effects from the SSOR. The central questions in this study are: could the risk to NKI sea otters from exposure to polycyclic aromatic hydrocarbons (PAHs) in SSOR, as characterized in 2001–2003, result in individual health effects, and, if so, could that exposure cause subpopulation-level effects? We follow the U.S. Environmental Protection Agency (USEPA) risk paradigm by: (a) identifying potential routes of exposure to PAHs from SSOR; (b) developing a quantitative simulation model of exposures using the best available scientific information; (c) developing scenarios based on calculated probabilities of sea otter exposures to SSOR; (d) simulating exposures for 500,000 modeled sea otters and extracting the 99.9% quantile most highly exposed individuals; and (e) comparing projected exposures to chronic toxicity reference values. Results indicate that, even under conservative assumptions in the model, maximum-exposed sea otters would not receive a dose of PAHs sufficient to cause any health effects; consequently, no plausible toxicological risk exists from SSOR to the sea otter subpopulation at NKI. 相似文献
968.
The active sites of the membrane-bound nucleoside triphosphate diphosphohydrolases (NTPDases) regulate and are regulated by
coordinated and spatially distant movements of their transmembrane helices, modulating enzyme activity, and substrate specificity.
Using site-directed mutagenesis, the roles of the conserved proline residues (N-terminal: P52 and P53; C-terminal: P472, P476,
P481, P484, and P485) of human NTPDase3, located in the “linker regions” that connect the N- and C-terminal transmembrane
helices with the extracellular active site, were examined. Single cysteine substitutions were strategically placed in the
transmembrane domain (N-terminal helix: V42C; C-terminal helix: G489C) to serve as cross-linking “sensors” of helical interactions.
These “sensor” background mutant proteins (V42C and G489C NTPDase3) are enzymatically active and are cross-linked by copper
phenanthroline less efficiently in the presence of adenosine triphosphate (ATP). Proline to alanine substitutions at P53,
P481, P484, and P485 in the V42C background, as well as P53, P481, and P484 in the G489C background, exhibited decreased nucleotidase
activities. More importantly, alanine substitutions at P53 and P481 in the V42C background and P481 in the G489C background
no longer exhibited the ATP-induced decrease in transmembrane cross-linking efficiency. Interestingly, the P485A mutation
abolished oxidative cross-linking at G489C both in the presence and absence of ATP. Taken together, these results suggest
a role for proline residues 53 and 481 in the linker regions of human NTPDase3 for coupling nucleotide binding at the enzyme
active site to movements and/or rearrangements of the transmembrane helices necessary for optimal nucleotide hydrolysis. 相似文献
969.
F1-ATPase, composed of α, β, γ, δ, and ? subunits, is a unique enzyme in terms of its rotational catalytic activity. The smallest unit showing this function is the α3β3γ complex. We have investigated the α3β3γ?ΔC (?ΔC, truncated ?) complex from thermophilic Bacillus PS3 (TF1′, 360 kDa) in the solution state by using the combination of extensive deuteration, segmental-labeling, and CRINEPT (cross-correlated relaxation-enhanced polarization transfer) NMR. Well-resolved CRINEPT-HMQC (heteronuclear multiple-quantum correlation) spectra of partially 15N-labeled TF1′ were obtained for this huge and asymmetric protein complex. The spectrum of the C-terminal domain of the β subunit revealed that the open form of the β subunit in the TF1′ complex is similar to that of the free β monomer. The open β subunit in the TF1′ complex does not exhibit high affinity for nucleotides unlike the monomer, but this is in agreement with the results of single-molecule analysis of TF1α3β3γ. On the other hand, the closed form of the β subunit in the TF1′ complex was shown to be distinct from that of the nucleotide-bound β monomer. This is consistent with a previous report that the closed form of the TF1β monomer could be a catalytically activated state. The loop between the N-terminal β-barrel and the central domain is highly flexible in the TF1′ complex, in contrast to that in the α3β3 hexamer, suggesting that it is affected by the presence of the γ subunit in this area. 相似文献
970.
Structure of the Flexible Amino-Terminal Domain of Prion Protein Bound to a Sulfated Glycan 总被引:1,自引:0,他引:1
Lara M. Taubner Ewa A. Bienkiewicz Byron Caughey 《Journal of molecular biology》2010,395(3):475-14017
The intrinsically disordered amino-proximal domain of hamster prion protein (PrP) contains four copies of a highly conserved octapeptide sequence, PHGGGWGQ, that is flanked by two polycationic residue clusters. This N-terminal domain mediates the binding of sulfated glycans, which can profoundly influence the conversion of PrP to pathological forms and the progression of prion disease. To investigate the structural consequences of sulfated glycan binding, we performed multidimensional heteronuclear (1H, 13C, 15N) NMR (nuclear magnetic resonance), circular dichroism (CD), and fluorescence studies on hamster PrP residues 23-106 (PrP 23-106) and fragments thereof when bound to pentosan polysulfate (PPS). While the majority of PrP 23-106 remain disordered upon PPS binding, the octarepeat region adopts a repeating loop-turn structure that we have determined by NMR. The β-like turns within the repeats are corroborated by CD data demonstrating that these turns are also present, although less pronounced, without PPS. Binding to PPS exposes a hydrophobic surface composed of aligned tryptophan side chains, the spacing and orientation of which are consistent with a self-association or ligand binding site. The unique tryptophan motif was probed by intrinsic tryptophan fluorescence, which displayed enhanced fluorescence of PrP 23-106 when bound to PPS, consistent with the alignment of tryptophan side chains. Chemical-shift mapping identified binding sites on PrP 23-106 for PPS, which include the octarepeat histidine and an N-terminal basic cluster previously linked to sulfated glycan binding. These data may in part explain how sulfated glycans modulate PrP conformational conversions and oligomerizations. 相似文献